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| yeast genetics book: Yeast Genetics Jeffrey S. Smith, Daniel J. Burke, 2016-09-17 Yeast Genetics: Methods and Protocols is a collection of methods to best study and manipulate Saccharomyces cerevisiae, a truly genetic powerhouse. The simple nature of a single cell eukaryotic organism, the relative ease of manipulating its genome and the ability to interchangeably exist in both haploid and diploid states have always made it an attractive model organism. Genes can be deleted, mutated, engineered and tagged at will. Saccharomyces cerevisiae has played a major role in the elucidation of multiple conserved cellular processes including MAP kinase signaling, splicing, transcription and many others. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Yeast Genetics: Methods and Protocols will provide a balanced blend of classic and more modern genetic methods relevant to a wide range of research areas and should be widely used as a reference in yeast labs. |
| yeast genetics book: Methods in Yeast Genetics Dan Burke, Dean Dawson, Tim Stearns, 2000 In the 2000 edition of this text, most of the experiments have been revised and several new experiments and methods have been added. These include: the 2-hybrid system, PCR-based methods and isolation of chromatin. |
| yeast genetics book: Molecular Genetics of Yeast John R. Johnston, 1994 The book covers comprehensively all current experimental procedures used in the research of the genetics and molecular biology of the yeast Saccharomyces cerevisiae. Featuring detailed protocols and practical tips, it guarantees easy access to a wide range of specialized topics within thisrapidly advancing field. Internationally-recognized experts present all methods currently in use, discussing topics such as DNA isolulation, cloning and expression vectors, cosmid cloning, construction and use of cDNA libraries, plasmid shuffling and mutant isolulation. Chapters on Ty insertionalmutagenesis, high efficiency transformation, cell-free translation of mRNAs, Ty virus-like particles, and applications to industrial strains of yeast are also included. Researchers in the fields of molecular biology, genetics, and biochemistry working with this yeast, as well as professionals of thebiotechnology industry will refer to this practical reference frequently. |
| yeast genetics book: Guide to Yeast Genetics and Molecular Biology , 2004-05-14 Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study Your Favorite Gene in yeast.Key Features* Basic Methods in Yeast Genetics* Physical and genetic mapping* Making and recovering mutants* Cloning and Recombinant DNA Methods* High-efficiency transformation* Preparation of yeast artificial chromosome vectors* Basic Methods of Cell Biology* Immunomicroscopy* Protein targeting assays* Biochemistry of Gene Expression* Vectors for regulated expression* Isolation of labeled and unlabeled DNA, RNA, and protein |
| yeast genetics book: Guide to Yeast Genetics: Functional Genomics, Proteomics, and Other Systems Analysis , 2010-02-27 This fully updated edition of the bestselling three-part Methods in Enzymology series, Guide to Yeast Genetics and Molecular Cell Biology is specifically designed to meet the needs of graduate students, postdoctoral students, and researchers by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable newcomers to set up a yeast laboratory and to master basic manipulations. This volume serves as an essential reference for any beginning or experienced researcher in the field. - Provides up-to-date methods necessary to study genes in yeast - Includes proceedures that enable newcomers to set up a yeast laboratory and to master basic manipulations - Serves as an essential reference for any beginning or experienced researcher in the field |
| yeast genetics book: Yeast Genetics J.F.T. Spencer, D.M. Spencer, A.R.W. Smith, 2012-12-06 During the past few decades we have witnessed an era of remarkable growth in the field of molecular biology. In 1950 very little was known of the chemical constitution of biological systems, the manner in which information was trans mitted from one organism to another, or the extent to which the chemical basis of life is unified. The picture today is dramatically different. We have an almost bewildering variety of information detailing many different aspects of life at the molecular level. There great advances have brought with them some breath-taking insights into the molecular mechanisms used by nature for rep licating, distributing and modifying biological information. We have learned a great deal about the chemical and physical nature of the macromolecular nucleic acids and proteins, and the manner in which carbohydrates, lipids and smaller molecules work together to provide the molecular setting of living sys tems. It might be said that these few decades have replaced a near vacuum of information with a very large surplus. It is in the context of this flood of information that this series of monographs on molecular biology has been organized. The idea is to bring together in one place, between the covers of one book, a concise assessment of the state of the subject in a well-defined field. This will enable the reader to get a sense of historical perspectiv(}-what is known about the field today-and a description of the frontiers of research where our knowledge is increasing steadily. |
| yeast genetics book: Yeast Horst Feldmann, 2010-01-11 Yeast is one of the oldest domesticated organisms and has both industrial and domestic applications. In addition, it is very widely used as a eukaryotic model organism in biological research and has offered valuable knowledge of genetics and basic cellular processes. In fact, studies in yeast have offered insight in mechanisms underlying ageing and diseases such as Alzheimers, Parkinsons and cancer. Yeast is also widely used in the lab as a tool for many technologies such as two-hybrid analysis, high throughput protein purification and localization and gene expression profiling. An up-to date resource providing a comprehensive account of yeast biology and its use as a tool and model organism for understanding cellular and molecular processes of eukaryotes. Topics covered range from the fundamentals of yeast biology such as cell structure, biochemistry, genetics and signaling, to current approaches and applications such as metabolomics, disease models and uses in biotechnology. |
| yeast genetics book: Yeast Horst Feldmann, 2012-09-06 Finally, a stand-alone, all-inclusive textbook on yeast biology. Based on the feedback resulting from his highly successful monograph, Horst Feldmann has totally rewritten he contents to produce a comprehensive, student-friendly textbook on the topic. The scope has been widened, with almost double the content so as to include all aspects of yeast biology, from genetics via cell biology right up to biotechnology applications. The cell and molecular biology sections have been vastly expanded, while information on other yeast species has been added, with contributions from additional authors. Naturally, the illustrations are in full color throughout, and the book is backed by a complimentary website. The resulting textbook caters to the needs of an increasing number of students in biomedical research, cell and molecular biology, microbiology and biotechnology who end up using yeast as an important tool or model organism. |
| yeast genetics book: Yeast Physiology and Biotechnology Graeme M. Walker, 1998-04-08 Yeasts are the world's premier industrial micro-organisms. In addition to their wide exploitation in the production of foods, beverages and pharmaceuticals, yeasts also play significant roles as model eukaryotic cells in furthering our knowledge in the biological and biomedical sciences. In order for modern biotechnology to fully exploit the activities of yeasts, it is essential to appreciate aspects of yeast cell physiology. In recent years, however, our knowledge of yeast physiological phenomena has lagged behind that of yeast genetics and molecular biology. Yeast Physiology and Biotechnology redresses the balance by linking key aspects of yeast physiology with yeast biotechnology. Individual chapters provide broad and timely coverage of yeast cytology, nutrition, growth and metabolism - important aspects of yeast cell physiology which are pertinent to the practical uses of yeasts in industry. The final chapter reviews traditional, modern and emerging biotechnologies in which roles of yeasts in the production of industrial commodities and their value in biomedical research are fully discussed. Relevant aspects of classical and modern yeast genetics and molecular biology are fully integrated into the appropriate chapters. This up-to-date and fully referenced book is aimed at advanced undergraduate and postgraduate bioscience students,but will also prove to be a valuable source of information for yeast researchers and technologists. |
| yeast genetics book: Yeast Genetic Engineering Philip J. Barr, Anthony J. Brake, Pablo Valenzuela, 1989 An illustration of the impressive development of yeast gene expression systems accomplished in recent years which demonstrates the power of these systems for the production of proteins in quantities useful as therapeutics, industrial enzymes and research agents. |
| yeast genetics book: Yeast Genetics John F.T. Spencer, Dorothy M. Spencer, I.J. Bruce, 2012-12-06 The manual consists of two main sections. The first includes the essential, sometimes laborious, procedures for handling yeasts, for inducing mating and isolation of hybrids, for inducing sporulation and isolation of single-spore clones, with some details of tetrad analysis, and including techniques and ancillary equipment for use of the micromanipulator. There are also proce dures for induction of mutants by physical and chemical agents, and for iso lation of particular types of mutants, such as to temperature sensitivity, for increased frequency of mutations, for mutations in the mitochondrial genome, both to the petite colonie form and to resistance to antibiotics, for mutations in that part of the yeast genome controlling the glycolytic cycle, and numerous others. Mapping of mutations is discussed briefly, though this aspect of yeast genetics is probably one which should not be undertaken until the investigator has gained a certain amount of experience in the field. How ever, as is pointed out in the pertinent part of the manual, the task of mapping has been tremendouslysimplified by the availability from the Yeast Genetics Stock Center at the University of California at Berkeley of a set of auxo trophic strains designed to permit mapping of most unknown genes with a minimum number of crosses and tetrad analyses. The first section concludes with the description of methods for hybridization of yeasts by protoplast fusion, which has been described as the poor man's system for genetic engineering. |
| yeast genetics book: Methods in Yeast Genetics Chris Kaiser, Susan Michaelis, Aaron Mitchell, 1994 |
| yeast genetics book: Molecular Biology of the Fission Yeast , 2012-12-02 This highly researched yeast, which represents a system used by cell biologists, geneticists and molecular biologists, has been given only minimal coverage in the literature. Its properties make it an excellent organism for DNA and related biotechnology reseach. This book, which is the first attempt to collate existing information in one source, will be an invaluable aid to those initiating projects with this organism. |
| yeast genetics book: Yeast Biotechnology: Diversity and Applications T. Satyanarayana, Gotthard Kunze, 2009-04-24 I belie ve that the book would provide an overview of the recent developments in the domain of yeast research with some new ideas, which could serve as an inspiration and challenge for researchers in this field. Ne w Delhi Prof. Asis Datta Dec. 24, 2007 F ormer Vice-chancellor, JNU Director, NCPGR (New Delhi) Pr eface Yeasts are eukaryotic unicellular microfungi that are widely distributed in the natural environments. Although yeasts are not as ubiquitous as bacteria in the na- ral environments, they have been isolated from terrestrial, aquatic and atmospheric environments. Yeast communities have been found in association with plants, a- mals and insects. Several species of yeasts have also been isolated from specialized or extreme environments like those with low water potential (e. g. high sugar/salt concentrations), low temperature (e. g. yeasts isolated from Antarctica), and low oxygen availability (e. g. intestinal tracts of animals). Around 1500 species of yeasts belonging to over 100 genera have been described so far. It is estimated that only 1% of the extant yeasts on earth have been described till date. Therefore, global efforts are underway to recover new yeast species from a variety of normal and extreme environments. Yeasts play an important role in food chains, and carbon, nitrogen and sulphur cycles. Yeasts can be genetically manipulated by hybridization, mutation, rare m- ing, cytoduction, spheroplast fusion, single chromosomal transfer and transfor- tion using recombinant technology. Yeasts (e. g. |
| yeast genetics book: Yeast Sugar Metabolism Friedrich K. Zimmermann, Karl-Dieter Entian, 2024-11-01 Yeast Sugar Metabolism looks at the biomechanics, genetics, biotechnology and applications of yeast sugar. The yeast Saccharomyces cereisiae has played a central role in the evolution of microbiology biochemistry and genetics, in addition to its use of a technical microbe for the production of alcoholic beverages and leavening of dough. |
| yeast genetics book: Yeast technology Gerald Reed, 2012-12-06 Yeasts are the active agents responsible for three of our most important foods - bread, wine, and beer - and for the almost universally used mind/ personality-altering drug, ethanol. Anthropologists have suggested that it was the production of ethanol that motivated primitive people to settle down and become farmers. The Earth is thought to be about 4. 5 billion years old. Fossil microorganisms have been found in Earth rock 3. 3 to 3. 5 billion years old. Microbes have been on Earth for that length of time carrying out their principal task of recycling organic matter as they still do today. Yeasts have most likely been on Earth for at least 2 billion years before humans arrived, and they playa key role in the conversion of sugars to alcohol and carbon dioxide. Early humans had no concept of either microorganisms or fermentation, yet the earliest historical records indicate that by 6000 B. C. they knew how to make bread, beer, and wine. Earliest humans were foragers who col lected andate leaves, tubers, fruits, berries, nuts, and cereal seeds most of the day much as apes do today in the wild. Crushed fruits readily undergo natural fermentation by indigenous yeasts, and moist seeds germinate and develop amylases that produce fermentable sugars. Honey, the first con centrated sweet known to humans, also spontaneously ferments to alcohol if it is by chance diluted with rainwater. Thus, yeasts and other microbes have had a long history of 2 to 3. |
| yeast genetics book: Yeast Genetics J. F. T. Spencer, D. M. Spencer, A. R. W. Smith, 1983-08-23 |
| yeast genetics book: The Early Days of Yeast Genetics Michael N. Hall, Patrick Linder, 2008 Yeast genetics began with Winge's 1935 studies of S. cerevisiae in Copenhagen, and afterwards was pursued by Lindegren in the U.S. and Ephrussi in France. Genetic studies in S. pombe were pioneered by Leupold in the 1940s in Switzerland. Within four decades, not without controversies, both yeast species were recognized as essential models in eukaryotic molecular cell biology. In this remarkable volume, Hall and Linder have assembled the reminiscences of many early investigators whose pioneering studies in the years before 1975 brought yeast biology to its current maturity. These illustrated essays about the science, the events and the personalities involved capture a fascinating era, in the informal style made famous by Phage and the Origins of Molecular Biology. This is a book that all scientists interested in the development of modern genetics and molecular biology should have on their shelves. |
| yeast genetics book: Guide to Yeast Genetics and Molecular and Cell Biology, Part C , 2002-06-14 This volume and its companion, Volume 350, are specifically designed to meet the needs of graduate students and postdoctoral students as well as researchers, by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable newcomers to set up a yeast laboratory and to master basic manipulations. Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines. Specific topics addressed in this book include cytology, biochemistry, cell fractionation, and cell biology. |
| yeast genetics book: Landmark Papers in Yeast Biology Patrick Linder, David Shore, Michael N. Hall, 2006 The yeasts have been important experimental organisms for more than 50 years. This volume contains over 100 selected papers, in sections with introductions that describe the process of discovery and the context and significance of the research. The selections include early classics as well as recent advances in areas such as signal transduction, membrane trafficking, protein turnover, and genomics. This book is designed as a guide for a literature-based course. |
| yeast genetics book: Guide to Yeast Genetics and Molecular and Cell Biology Christine Guthrie, Gerald R. Fink, 2004 |
| yeast genetics book: Yeast Stress Responses Stefan Hohmann, Willem H. Mager, 2007-10-23 Every cell has developed mechanisms to respond to changes in its environment and to adapt its growth and metabolism to unfavorable conditions. The unicellular eukaryote yeast has long proven as a particularly useful model system for the analysis of cellular stress responses, and the completion of the yeast genome sequence has only added to its power This volume comprehensively reviews both the basic features of the yeast genral stress response and the specific adapations to different stress types (nutrient depletion, osmotic and heat shock as well as salt and oxidative stress). It includes the latest findings in the field and discusses the implications for the analysis of stress response mechanisms in higher eukaryotes as well. |
| yeast genetics book: The Yeast Two-hybrid System Paul L. Bartel, Stanley Fields, 1997 Biologists have already identified thousands of new gene sequences, and genome sequencing efforts are speeding up the discovery process even further. With this explosion of sequence information comes the need to understand how genes work in concert in order to fulfill the cells functions. The yeast two-hybrid system--used to identify protein-protein interactions--is one of the most powerful and versatile methods for characterizing a protein's function. It has become an essential tool for both academic researchers and those in biotechnology and pharmaceutical companies. This volume presents work by pioneers in the field and is the first publication devoted solely to the yeast two-hybrid system. It includes detailed protocols, practical advice on troubleshooting, and suggestions for future development. In addition, it explains how to construct an activation domain hybrid library, how to identify mutations that disrupt an interaction, and how to use the system in mammalian cells. Many of the contributors have developed new applications and variations of the technique. Chapter topics include characterizing hormone/receptor complexes, identifying peptide ligands, analyzing interactions mediated by protein modifications, and dissecting the cell cycle and other complex genetic networks. The Yeast Two-Hybrid System is the single complete resource for scientists interested in this powerful research method. |
| yeast genetics book: Metabolism and Molecular Physiology of Saccharomyces Cerevisiae J Richardson Dickinson, 1998-12-09 This text emphasises the importance of staying informed about Saccharomyces cerevisiae as it provides the intellectual basis for much of the molecular and cellular biology of eukaryotes. It offers yeast users a concise account of the metabolism and physiology of this organism. Chapters include: life cycle and morphogenesis; carbon metabolism, nitro |
| yeast genetics book: The Marriage Plot Jeffrey Eugenides, 2011-10-11 A New York Times Notable Book of 2011 A Publisher's Weekly Top 10 Book of 2011 A Kirkus Reviews Top 25 Best Fiction of 2011 Title One of Library Journal's Best Books of 2011 A Salon Best Fiction of 2011 title One of The Telegraph's Best Fiction Books of the Year 2011 It's the early 1980s—the country is in a deep recession, and life after college is harder than ever. In the cafés on College Hill, the wised-up kids are inhaling Derrida and listening to Talking Heads. But Madeleine Hanna, dutiful English major, is writing her senior thesis on Jane Austen and George Eliot, purveyors of the marriage plot that lies at the heart of the greatest English novels. As Madeleine tries to understand why it became laughable to read writers like Cheever and Updike, who wrote about the suburbia Madeleine and most of her friends had grown up in, in favor of reading the Marquis de Sade, who wrote about deflowering virgins in eighteenth-century France, real life, in the form of two very different guys, intervenes. Leonard Bankhead—charismatic loner, college Darwinist, and lost Portland boy—suddenly turns up in a semiotics seminar, and soon Madeleine finds herself in a highly charged erotic and intellectual relationship with him. At the same time, her old friend Mitchell Grammaticus—who's been reading Christian mysticism and generally acting strange—resurfaces, obsessed with the idea that Madeleine is destined to be his mate. Over the next year, as the members of the triangle in this amazing, spellbinding novel graduate from college and enter the real world, events force them to reevaluate everything they learned in school. Leonard and Madeleine move to a biology Laboratory on Cape Cod, but can't escape the secret responsible for Leonard's seemingly inexhaustible energy and plunging moods. And Mitchell, traveling around the world to get Madeleine out of his mind, finds himself face-to-face with ultimate questions about the meaning of life, the existence of God, and the true nature of love. Are the great love stories of the nineteenth century dead? Or can there be a new story, written for today and alive to the realities of feminism, sexual freedom, prenups, and divorce? With devastating wit and an abiding understanding of and affection for his characters, Jeffrey Eugenides revives the motivating energies of the Novel, while creating a story so contemporary and fresh that it reads like the intimate journal of our own lives. |
| yeast genetics book: From a to [alpha] Hiten D. Madhani, 2007 & From a to alphais a short supplemental textbook that uses control of yeast mating type as a model for many aspects of cell determination in general. Topics covered include gene silencing; genetic recombination; differentiation; combinatorial gene regulation; mRNA transport to establish asymmetric cell division; signal transduction; evolution of genetic networks; and various aspects of cell biology, including action of cytoskeleton and bud site selection. The book includes a foreword by Mark Ptashne, author of A Genetic Switch. |
| yeast genetics book: Non-conventional Yeasts: from Basic Research to Application Andriy Sibirny, 2019-08-12 This volume scopes several aspects of non-conventional yeast research prepared by the leading specialists in the field. An introduction on taxonomy and systematics enhances the reader’s knowledge on yeasts beyond established ones such as Saccharomyces cerevisiae. Biotechnological approaches that involve fungal utilization of unusual substrates, production of biofuels and useful chemicals as citric acid, glutathione or erythritol are discussed. Further, strategies for metabolic engineering based on knowledge on regulation of gene expression as well as sensing and signaling pathways are presented. The book targets researchers and advanced students working in Microbiology, Microbial Biotechnology and Biochemistry. |
| yeast genetics book: Methods in Yeast Genetics , 1981 |
| yeast genetics book: Genetic Techniques for Biological Research Corinne A. Michels, 2002-06-10 Genetic Techniques for Biological Research ist ein Lehrbuch für fortgeschrittene Studenten und Doktoranden der Genetik, Molekularbiologie und Zellbiologie. Es basiert auf Fallstudien zur Hefe Saccharomyces als genetischem Modellorganismus, an dem anschaulich Theorie und Praxis der molekulargenetischen Analyse demonstriert wird. Darüber hinaus bietet es dem Leser umfassende Informationen, damit er diesen Ansatz in seine eigenen Forschungsprojekte einbauen kann. Autorin Corinne Michels - eine Expertin auf dem Gebiet der Hefegenetik und Molekularbiologie - erklärt hier genau, wie man praktische genetische Studien mit Hilfe von durchgearbeiteten Beispielen kritisch bewertet. Auf diese Weise soll der Leser die Fähigkeit zu kritischem Denken entwickeln, um das Material in eigenen Forschungsarbeiten anwenden zu können. Ein idealer Studienbegleiter zu Theorie und Praxis der molekulargenetischen Analyse! |
| yeast genetics book: Guide to Yeast Genetics and Molecular Cell Biology, Part B , 2002-06-12 This volume and its companion, Volume 351, are specifically designed to meet the needs of graduate students and postdoctoral students as well as researchers, by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable newcomers to set up a yeast laboratory and to master basic manipulations. Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines. Specific topics addressed in this book include basic techniques, making mutants, genomics, and proteomics. |
| yeast genetics book: Yeast Systems Biology Juan I. Castrillo, Stephen G. Oliver, 2011-08-23 Systems Biology aims at deciphering the genotype-phenotype relationships at the levels of genes, transcripts (RNAs), peptides, proteins, metabolites, and environmental factors participating in complex cellular networks in order to reveal the mechanisms and principles governing the behavior of complex biological systems. Yeast Systems Biology: Methods and Protocols presents an up-to-date view of the optimal characteristics of the yeast Saccharomyces cerevisiae as a model eukaryote, perspective on the latest experimental and computational techniques for systems biology studies, most of which were first designed for and validated in yeast, and selected examples of yeast systems biology studies and their applications in biotechnology and medicine. These experiments under controlled conditions can uncover the complexity and interplay of biological networks with their dynamics, basic principles of internal organization, and balanced orchestrated functions between organelles in direct interaction with the environment as well as the characterization of short and long-term effects of perturbations and dysregulation of networks that may illuminate the origin of complex human diseases. Written for the highly successful Methods in Molecular BiologyTM series, this volume contains the kind of detailed description and implementation advice that is crucial for getting optimal results. Practical and cutting-edge, Yeast Systems Biology: Methods and Protocols serves researchers interested in comprehensive systems biology strategies in well-defined model systems with specific objectives as well as a better knowledge of the latest post-genomic strategies at all ‘omic levels and computational approaches towards analysis, integration, and modeling of biological systems, from single-celled organisms to higher eukaryotes. |
| yeast genetics book: Budding Yeast Brenda Andrews, Charles M. Boone, Trisha Davis, Stanley Fields, 2016 Over the past century, studies of the budding yeast Saccharomyces cerevisiae have helped to unravel principles of nearly every aspect of eukaryotic cell biologyfrom metabolism and molecular genetics to cell division and differentiation. Thanks to its short generation time, ease of genetic manipulation, and suitability for high-throughput studies, yeast remains the focus of research in a vast number of laboratories worldwide. This laboratory manual provides a comprehensive collection of experimental procedures that continue to make budding yeast an informative model. The contributors describe methods for culturing and genetically modifying yeast, strategies and tools (e.g., gene deletion collections) for functional analyses, approaches for characterizing cell structure and morphology, and techniques to probe the modifications and interactions of various cellular constituents (e.g., using one- and two-hybrid screens). Strategies for studying metabolomics, complex traits, and evolution in yeast are also covered, as are methods to isolate and investigate new strains of yeast from the wild. Several additional chapters are devoted to bioinformatics tools and resources for yeast biology (e.g., the Saccharomyces Genome Database). This manual is therefore an essential resource for all researchers, from graduate level upward, who use budding yeast to explore the intricate workings of cells. |
| yeast genetics book: Methods in Yeast Genetics Fred Sherman, 1979 |
| yeast genetics book: Molecular Mechanisms in Yeast Carbon Metabolism Jure Piškur, Concetta Compagno, 2014-05-06 Yeast is one of the most studied laboratory organisms and represents one of the most central models to understand how any eukaryote cell works. On the other hand, yeast fermentations have for millennia provided us with a variety of biotech products, like wine, beer, vitamins, and recently also with pharmaceutically active heterologous products and biofuels. A central biochemical activity in the yeast cell is the metabolism of carbon compounds, providing energy for the whole cell, and precursors for any of the final fermentation products. A complex set of genes and regulatory pathways controls the metabolism of carbon compounds, from nutrient sensing, signal transduction, transcription regulation and post-transcriptional events. Recent advances in comparative genomics and development of post-genomic tools have provided further insights into the network of genes and enzymes, and molecular mechanisms which are responsible for a balanced metabolism of carbon compounds in the yeast cell, and which could be manipulated in the laboratory to increase the yield and quality of yeast biotech products. This book provides a dozen of most comprehensive reviews on the recent developments and achievements in the field of yeast carbon metabolism, from academic studies on gene expression to biotechnology relevant topics. |
| yeast genetics book: Yeast Gene Analysis , 2007-07-09 Focusing on Saccharomyces cerevisiae, the second edition of Yeast Gene Analysis represents a major reworking of the original edition, with many completely new chapters and major revisions to all previous chapters. Originally published shortly after completion of the yeast genome sequence, the new edition covers many of the major genome-wide strategies that have been developed since then such as microarray analysis of transcription, synthetic gene array studies, protein microarrays and chemical genetic approaches. It represents a valuable resource for any research laboratory using budding yeast as their experimental system in which to identify new yeast gene functions. The chapters are written in a readable style with useful background information, technical tips and specific experimental protocols included as appropriate, enabling both the novice and the experienced yeast researcher to adopt new procedures with confidence. |
| yeast genetics book: Guide to Yeast Genetics and Molecular Biology Christine Guthrie, Gerald R. Fink, 1991-01-28 Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study Your Favorite Gene in yeast. Basic Methods in Yeast Genetics**Physical and genetic mapping**Making and recovering mutants**Cloning and Recombinant DNA Methods**High-efficiency transformation**Preparation of yeast artificial chromosome vectors**Basic Methods of Cell Biology**Immunomicroscopy**Protein targeting assays**Biochemistry of Gene Expression**Vectors for regulated expression**Isolation of labeled and unlabeled DNA, RNA, and protein |
| yeast genetics book: Yeast Diversity in Human Welfare Tulasi Satyanarayana, Gotthard Kunze, 2017-05-13 This book brings together and updates the latest information on the diversity of yeasts, their molecular features and their applications in the welfare of mankind. Yeasts are eukaryotic microfungi widely found in natural environments, including those with extreme conditions such as low temperatures, low oxygen levels and low water availability. To date, approximately 2,000 of the estimated 30,000 to 45,000 species of yeast on Earth, belonging to around 200 genera have been described. Although there are a few that are opportunistic human and animal pathogens, the vast majority of yeasts are beneficial, playing an important role in the food chain and in the carbon, nitrogen and sulphur cycles. In addition, yeasts such as Saccharomyces cerevisiae, Hansenula polymorpha and Pichia pastoris are used in expressing foreign genes to produce proteins of pharmaceutical interest. A landmark in biotechnology was reached in 1996 with the completion of sequencing of the entire S. cerevisiae genome, and it has now become a central player in the development of an entirely new approach to biological research and synthetic biology. The sequencing of genomes of several yeasts including Schizosaccharomyces pombe, Candida albicans and Cryptococcus neofromans has also recently been completed. candida albicans= and= p/pp |
| yeast genetics book: Yeast Protocols Ivor Howell Evans, 1996 Yeast Protocols contains many key techniques for studying the biology of yeasts at both the cellular and molecular levels. Working primarily from Saccharomyces cerevisiae, the expert contributors explain step-by-step how to successfully isolate, identify, and culture yeasts; the secrets of meiotic mapping; how to use PFGE in karyotyping and gene localization; the methods for purification and analysis of various cell components; and the construction and exploitation of genomic DNA clone banks. They also cover the latest methods for chromosome engineering, insertional mutagenesis by Ty elements, mRNA abundance and half-life measurements, the use of reporter gene systems, genotoxicity testing, and more. Yeast Protocols follows the widely applauded Humana Methods in Molecular Biology style: brief introductions putting the particular method in context, comprehensive lists of materials, cookbook style instructions, and troubleshooting notes to avoid common pitfalls and solve problems. The techniques can be used with confidence and success by both inexperienced newcomers and established researchers. |
Homemade Natural Yeast Nutrient Recipe - Homebrew Talk
Sep 22, 2013 · Craft A Brew - Safale WB-06 Dry Yeast - German Wheat Beer Dry Yeast - for Wheat Beers - Ingredients for Home Brewing - Beer Making Supplies - 1 Pack Craft a Brew …
Process to add yeast nutrient later in fermentation?
Mar 21, 2013 · The basics are that yeast need YAN (Yeast Assimilable Nitrogen) for fermentation and most apples inherently have enough YAN for the yeast to complete fermentation. …
How Many Cells in a Yeast Slurry. Anyone Use This Method?
Feb 16, 2012 · There also is a finite limit to total viable yeast cells that can exist in a ml of yeast slurry. At some point the only way to increase the number of viable yeast cells is to increase …
Belgian yeast strains | Homebrew Talk - Beer, Wine, Mead,
Oct 24, 2024 · I'm just skeptical, I've read that Fermentis don't sell yeast to repackers and I think MJ yeasts generally fit the Lalbrew range. And BE-256 is a pale coloured yeast. As a …
ESTIMATING YEAST DENSITY - Homebrew Talk
Feb 10, 2019 · From the book "Yeast" by White and Zainasheff, a 'pure' yeast slurry that has been refrigerated and the yeast has compacted in bottom of the vessel, the slurry is around 8 billion …
Dry Yeast, Liquid Yeast Equivalents - Homebrew Talk
Jul 22, 2012 · Danstar Munich Wheat Beer yeast originated in Bavaria, Germany, the home of many of the world s great wheat beer breweries. The propagation and drying processes for …
Dry Kolsch Yeast Recommendations - Homebrew Talk
Jun 29, 2022 · After switching to LalBrew Premium Kolsch Style Ale dry yeast from White Labs - WLP029 - German/Kölsch liquid yeast, I will never go back. LalBrew ferments clean and, …
Japanese yeast for lager - Homebrew Talk
Dec 17, 2014 · ive always thought you need actual japanese yeast. so to that end i wonder if anyone has been able to get a hand on some actual japanese lager yeast? i cant find a damn …
Higher temp yeast's - Homebrew Talk
Oct 18, 2024 · Many come in dry form, for ease of use. Stay away from liquid yeast if they need to be shipped, which is best done during much cool(er) weather. Some form of temperature …
CellarScience Nectar yeast - Homebrew Talk
Jun 1, 2022 · I have used Nectar yeast twice now. First batch i did a pale ale with centential citra and amarillo. First time i fermented low end of the spectrum and did not get any belgian type …
Homemade Natural Yeast Nutrient Recipe - Homebrew Talk
Sep 22, 2013 · Craft A Brew - Safale WB-06 Dry Yeast - German Wheat Beer Dry Yeast - for Wheat Beers - Ingredients for Home Brewing - Beer Making Supplies - 1 Pack Craft a Brew …
Process to add yeast nutrient later in fermentation?
Mar 21, 2013 · The basics are that yeast need YAN (Yeast Assimilable Nitrogen) for fermentation and most apples inherently have enough YAN for the yeast to complete fermentation. …
How Many Cells in a Yeast Slurry. Anyone Use This Method?
Feb 16, 2012 · There also is a finite limit to total viable yeast cells that can exist in a ml of yeast slurry. At some point the only way to increase the number of viable yeast cells is to increase …
Belgian yeast strains | Homebrew Talk - Beer, Wine, Mead, & Cider ...
Oct 24, 2024 · I'm just skeptical, I've read that Fermentis don't sell yeast to repackers and I think MJ yeasts generally fit the Lalbrew range. And BE-256 is a pale coloured yeast. As a …
ESTIMATING YEAST DENSITY - Homebrew Talk
Feb 10, 2019 · From the book "Yeast" by White and Zainasheff, a 'pure' yeast slurry that has been refrigerated and the yeast has compacted in bottom of the vessel, the slurry is around 8 billion …
Dry Yeast, Liquid Yeast Equivalents - Homebrew Talk
Jul 22, 2012 · Danstar Munich Wheat Beer yeast originated in Bavaria, Germany, the home of many of the world s great wheat beer breweries. The propagation and drying processes for …
Dry Kolsch Yeast Recommendations - Homebrew Talk
Jun 29, 2022 · After switching to LalBrew Premium Kolsch Style Ale dry yeast from White Labs - WLP029 - German/Kölsch liquid yeast, I will never go back. LalBrew ferments clean and, …
Japanese yeast for lager - Homebrew Talk
Dec 17, 2014 · ive always thought you need actual japanese yeast. so to that end i wonder if anyone has been able to get a hand on some actual japanese lager yeast? i cant find a damn …
Higher temp yeast's - Homebrew Talk
Oct 18, 2024 · Many come in dry form, for ease of use. Stay away from liquid yeast if they need to be shipped, which is best done during much cool(er) weather. Some form of temperature …
CellarScience Nectar yeast - Homebrew Talk
Jun 1, 2022 · I have used Nectar yeast twice now. First batch i did a pale ale with centential citra and amarillo. First time i fermented low end of the spectrum and did not get any belgian type …
