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| zb50 clone: The Journal of Cell Biology , 1996 No. 2, pt. 2 of November issue each year from v. 19 (1963)-47 (1970) and v. 55 (1972)- contain the Abstracts of papers presented at the Annual Meeting of the American Society for Cell Biology, 3d (1963)-10th (1970) and 12th (1972)- |
| zb50 clone: Comparative Medicine , 2007 |
| zb50 clone: A Concise Dictionary of the Assyrian Language William Muss-Arnolt, 1905 |
| zb50 clone: Audio Cyclopedia Howard M. Tremaine, 1977 |
| zb50 clone: Yeast Metabolic Engineering Valeria Mapelli, 2016-08-23 Yeast Metabolic Engineering: Methods and Protocols provides the widely established basic tools used in yeast metabolic engineering, while describing in deeper detail novel and innovative methods that have valuable potential to improve metabolic engineering strategies in industrial biotechnology applications. Beginning with an extensive section on molecular tools and technology for yeast engineering, this detailed volume is not limited to methods for Saccharomyces cerevisiae, but describes tools and protocols for engineering other yeasts of biotechnological interest, such as Pichia pastoris, Hansenula polymorpha and Zygosaccharomyces bailii. Tools and technologies for the investigation and determination of yeast metabolic features are described in detail as well as metabolic models and their application for yeast metabolic engineering, while a chapter describing patenting and regulations with a special glance at yeast biotechnology closes the volume. Written in the highly successful Methods in Molecular Biology series format, most chapters include an introduction to their respective topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Yeast Metabolic Engineering: Methods and Protocols aims to familiarize researchers with the current state of these vital and increasingly useful technologies. |
| zb50 clone: Star Wars George Lucas, Laurent Bouzereau, 1998 This volume contains all three full-length Star Wars screenplays, presented with the secrets that led to their creation. Through interviews with such people as George Lucas and Lawrence Kasdan, the author uncovers the complex process through which the Star Wars saga came to the screen. |
| zb50 clone: Capillary Electrophoresis of Proteins and Peptides Mark A. Strege, Avinash L. Lagu, 2008-02-04 Throughout the more than 20 years that have followed the beginnings of capillary electrophoresis (CE), its application to the analysis of proteins and peptides has continued to be reliable, versatile, and productive. Over time, CE has matured to become a superb complement to HLPC, and in many cases has also evolved as an automated and quantitative replacement for conventional slab gel electrophoresis methods such as SDS-PAGE and isoelectric focusing. Within Capillary Electrophoresis of Proteins and Peptides, we have assembled contributions from researchers who are applying state-of-the-art CE for protein and peptide analysis, including topics that we believe are of great potential both in the present and for the future. In comparison to traditional separation methods, CE represents a miniaturized analysis technique (especially in its microchip-based format) that is highly dependent upon the basic fundamentals of effective sample recovery and high sensitivity detection. With these issues in mind, Chapters 1–4 describe recently developed approaches for both capillary coatings and analyte detection via laser-induced fluorescence. Since the discipline of biotechnology has established itself as a primary platform for the application of CE to the analysis of proteins and peptides, Chapters 5–7 demonstrate a variety of examples of the specific techniques that have been applied for the development of biopharmaceuticals and their commercialization. The methods covered here include also the analysis of oligosaccharides from glycoproteins. |
| zb50 clone: Men and Saints Charles Péguy, 1944 |
| zb50 clone: Mr Noon D. H. Lawrence, 1984-09-13 Mr Noon is a sardonic tale about the amorous adventures of Gilbert Noon, a young schoolmaster in Lawrence's home county of Nottinghamshire who gets entangled with a girl, loses his job, and decides to leave the country to escape the narrow provincial middle-class morality. It was first known as a long story posthumously published in A Modern Lover (1934) and collected in the volume called Phoenix II (1968). Lawrence in fact wrote a long continuation of the novel, but the manuscript disappeared for many years. The Cambridge edition brought the two parts together for the first time. It is like a sequel to Sons and Lovers, but much more straightforwardly autobiographical. The publication of the complete work added a new work of major importance to the canon of a great writer, and was widely hailed as a major literary event. |
| zb50 clone: Expression Systems Michael R. Dyson, Yves Durocher, 2007 Protein expression is an increasingly important tool for research on gene function. What is needed is not just a lab manual providing established methods as well as the latest state-of-the-art protocols, but also clear advice on what expression system to choose when. Expression Systems: Methods Expressuniquely fills this need. It covers expression across a broad range of systems, including the following. *Baculovirus expression vectors *CHO cells *E. coli *HEK293-EBNA1 cells *Lactococcus lactis and other gram positive bacteria *S. cerevisiae *transfected insect cells *Pichia pastoris *mammalian cells using BacMam viruses *lentiviral vectors *wheat germ cell-free system The book takes the reader through how to make an informed choice of appropriate system, taking into account the protein target, the time involved, the ultimate use of the expressed protein, and the laboratory equipment required. It also provides step-by-step methods for each system. In addition, the book describes the optimisation of expression strategies, expression engineering using ribosome display, and how to select protein variants with improved expression. Every chapter discusses the merits and limitations of the approaches available, describes the key techniques in full practical detail, and provides sensible advice for immediate use at the bench. In summary, Expression Systems: Methods Expressis a comprehensive laboratory manual and information resource for researchers at all levels, from postgraduate student to principal investigator. |
| zb50 clone: Metabolic Pathway Engineering Michael E. Himmel, Yannick J. Bomble, 2020-07-28 This book illustrates experimental and computational methodologies used to achieve cost effective biological processes for the production of fuels and biochemicals through multiple approaches to increasing yield, titers, and productivity in a robust host. The volume includes the most recent and cutting-edge aspects of pathway engineering, flux analysis, and metabolic enzyme engineering. Each chapter highlights the complexity and challenges of the problem as well as the methods used to solve this problem or changes needed in current methods. As a part of the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice that gives researchers a much needed boost. Authoritative and practical, Metabolic Pathway Engineering benefits not only scientists working on more fundamental aspects of this endeavor but also those in the biochemical industry working on strain engineering for robust industrial processes. |
| zb50 clone: Animal Cell Culture Mohamed Al-Rubeai, 2014-11-28 Animal cells are the preferred “cell factories” for the production of complex molecules and antibodies for use as prophylactics, therapeutics or diagnostics. Animal cells are required for the correct post-translational processing (including glycosylation) of biopharmaceutical protein products. They are used for the production of viral vectors for gene therapy. Major targets for this therapy include cancer, HIV, arthritis, cardiovascular and CNS diseases and cystic fibrosis. Animal cells are used as in vitro substrates in pharmacological and toxicological studies. This book is designed to serve as a comprehensive review of animal cell culture, covering the current status of both research and applications. For the student or R&D scientist or new researcher the protocols are central to the performance of cell culture work, yet a broad understanding is essential for translation of laboratory findings into the industrial production. Within the broad scope of the book, each topic is reviewed authoritatively by experts in the field to produce state-of-the-art collection of current research. A major reference volume on cell culture research and how it impacts on production of biopharmaceutical proteins worldwide, the book is essential reading for everyone working in cell culture and is a recommended volume for all biotechnology libraries. |
| zb50 clone: Microbial Metabolic Engineering Christine Nicole S. Santos, Parayil Kumaran Ajikumar, 2019-02-21 This volume covers a wide array of topics that will aid researchers in the task of engineering complex biological systems. This book is divided into three parts: Part One discusses the discovery and identification of relevant biosynthetic pathways for engineering; Part Two looks at the development of genetic tools for manipulating enzymes, biosynthetic pathways, and whole genomes; and Part Three covers the characterization of engineered microbes using targeted and global systems biology tools, as well as in silico models. Chapters explore topics such as leveraging enzyme promiscuity to construct novel biosynthetic pathways; assembling combinatorial multigene pathways for rapid strain optimization; applying 'omics technologies for identifying bottlenecks; and engineering nontraditional host organisms like cyanobacterium and Yarrowia lipolytica. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and authoritative, Microbial Metabolic Engineering: Methods and Protocols is a valuable resource for researchers and scientists interested in engineering and optimizing microbes for a variety of biotechnological applications. |
| zb50 clone: The phagocytic cell in host resistance Joseph A. Bellanti, 1975 |
| zb50 clone: Protein Hydrolysates in Biotechnology Vijai K. Pasupuleti, Arnold L. Demain, 2010-08-28 Protein hydrolysates, otherwise commonly known as peptones or peptides, are used in a wide variety of products in fermentation and biotechnology industries. The term “peptone” was first introduced in 1880 by Nagelli for growing bacterial cultures. However, later it was discovered that peptones derived from the partial digestion of proteins would furnish organic nitrogen in readily available form. Ever since, p- tones, which are commonly known as protein hydrolysates, have been used not only for growth of microbial cultures, but also as nitrogen source in commercial fermen- tions using animal cells and recombinant microorganisms for the production of value added products such as therapeutic proteins, hormones, vaccines, etc. Today, the characterization, screening and manufacturing of protein hyd- lysates has become more sophisticated, with the introduction of reliable analytical instrumentation, high throughput screening techniques coupled with statistical design approaches, novel enzymes and efficient downstream processing equipment. This has enabled the introduction of custom-built products for specialized appli- tions in diverse fields of fermentation and biotechnology, such as the following. 1. Protein hydrolysates are used as much more than a simple nitrogen source. For example, the productivities of several therapeutic drugs made by animal cells and recombinant microorganisms have been markedly increased by use of p- tein hydrolysates. This is extremely important when capacities are limited. 2. Protein hydrolysates are employed in the manufacturing of vaccines by ferm- tation processes and also used as vaccine stabilizers. |
| zb50 clone: Checkpoint Controls and Cancer Axel H. Schönthal, 2004-06-24 Intracellular checkpoint controls constitute a network of signal transd- tion pathways that protect cells from external stresses and internal errors. Ext- nal stresses can be generated by the continuous assault of DNA-damaging agents, such as environmental mutagens, ultraviolet (UV) light, ionizing radiation, or the reactive oxygen species that can arise during normal cellular metabolism. In response to any of these assaults on the integrity of the genome, the activation of the network of checkpoint control pathways can lead to diverse cellular responses, such as cell cycle arrest, DNA repair, or elimination of the cell by cell death (apoptosis) if the damage cannot be repaired. Moreover, internal errors can occur during the highly orchestrated replication of the cellular genome and its distribution into daughter cells. Here, the temporal order of these cell cycle events must be strictly enforced—for example, to ensure that DNA replication is c- plete and occurs only once before cell division, or to monitor mitotic spindle assembly, and to prevent exit from mitosis until chromosome segregation has been completed. Thus, well functioning checkpoint mechanisms are central to the maintenance of genomic integrity and the basic viability of cells and, the- fore, are essential for proper development and survival. The importance of proper functioning of checkpoints becomes plainly obvious under conditions in which this control network malfunctions and fails. Depending on the severity and timing, failure of this machinery can lead to embryonic lethality, genetic diseases, and cancer. |
| zb50 clone: Animal Cell Technology Manuel J.T. Carrondo, Bryan Griffiths, José L.P. Moreira, 2012-12-06 Animal cell technology has undergone a rapid transformation over the last decade from a research tool and highly specialised technology to a central resource for innovation in pharmaceutical research and development. These proceedings of the 14th Meeting of the European Society for Animal Cell Technology (Vilamoura, Portugal, May 1996) bring up to date the historical perspective of animal cell technology for the benefit of society, `From Vaccines to Genetic Medicine', and will charter this vital technology for the years to come. Strong contributions are grouped in the traditional ESACT areas of 'Cell and Physiology Engineering' dealing with cell state, including genetics, and its environment, and 'Animal Cell Process Engineering' covering integration of bioreaction with bioseparation coupled with on-line monitoring to improve protein production and consistency. Extensive coverage of metabolic engineering on synthesis, folding, assembly, transiting and secretion is dealt with in the session on 'Recombinant Proteins: Biosynthesis and Bioprocessing'. Two traditional but expanding areas of animal cell technology relevance are highlighted in the broad sessions of 'Animal Cells as Tools for Discovery and Testing' and 'Animal Cell Vaccines: Present and Future'. Two sessions finally cover the more recent domains of animal cell technology work - 'Tissue Engineering and Biomedical Devices' and 'Cells and Vectors for Genetic Medicine' - where one can foresee a very bright future. |
| zb50 clone: Animal Cell Biotechnology Raymond Spier, J. B. Griffiths, 1985 |
| zb50 clone: Texts in the Babylonian Wedge-writing Theophilus Goldridge Pinches, 1880 |
| zb50 clone: Babylonian Magic and Sorcery Leonard W. King, 2000-09-15 Originally published in 1896, this text contains the cunieform text of 60 clay tablets written between 669-625 BC. These tablets were inscribed with prayers and religious compositions of a devotional and magical character and there is little doubt that they were compiled from Babylonian sources. |
| zb50 clone: Assyrian and Babylonian Letters Belonging to the Kouyunjik Collections of the British Museum Robert Francis Harper, 1892 |
| zb50 clone: Assyrische Lesestücke Friedrich Delitzsch, 1900 |
| zb50 clone: An Assyrian Manual D. G. Lyon, 1886 |
| zb50 clone: The Code of Hammurabi Robert Francis Harper, 2007-11-01 The intention of Ancient Texts and Translations (ATT) is to make available a variety of ancient documents and document collections to a broad range of readers. The series will include reprints of long out-of- print volumes, revisions of earlier editions, and completely new volumes. The understanding of ancient societies depends upon our close reading of the documents, however fragmentary, that have survived. --K. C. Hanson Series Editor |
| zb50 clone: The Babylonian and Oriental Record , 1894 |
| zb50 clone: Proceedings of the American Oriental Society American Oriental Society, 1866 |
| zb50 clone: Clone #1 David Schulner, 2012-11-21 From ROBERT KIRKMAN's Skybound imprint, comes a sci-fi story like you've never seen before! Dr. Luke Taylor's perfect life comes to a dramatic halt when an identical, bloodied version of himself arrives at his doorstep with news that he is one of many clones and they're all after his pregnant wife and their unborn child! |
| zb50 clone: The Clone Apocalypse Steven L. Kent, 2014-11-25 Earth, A.D. 2519. The clone soldiers of the Enlisted Man’s Empire, formerly members of the Unified Authority’s powerful military, believe they have finally secured their freedom. They may not live to learn how wrong they are… After launching an unsuccessful invasion of Washington, D.C., the Unified Authority is on the verge of defeat. Then the clones intercept a message detailing the U.A.’s last ditch plan for survival: a super weapon, a virus designed to attack the clones’ internal architecture. Only one clone was created without the fatal flaw—Wayson Harris, an outlaw model with independent thoughts and an addiction to violence. As his empire collapses and his comrades die around him, Harris begins a one-man war against the government that created, betrayed and ultimately destroyed his brothers. Fighting the war becomes more difficult, however, as the rush from the constant combat has reached its peak—and is driving Harris slowly insane… |
| zb50 clone: A Classified List of All Simple and Compound Cuneiform Ideographs Occurring in the Texts Hitherto Published with Their Assyro-Babylonian Equivalents, Phonetic Values Etc Brünnow, 1889 |
| zb50 clone: The Clone Assassin Steven L. Kent, 2013-10-29 Earth, A.D. 2519. The clone soldiers of the Enlisted Man’s Empire, formerly members of the Unified Authority’s powerful military, maintain a tenuous grasp on the power they fought so hard to gain. But the U.A. will not be so easy to suppress as they had hoped… A provocateur attacks the Pentagon. Gunships converge on the penitentiary where Unified Authority war criminals are held. And a clone assassin murders Admiral Don Cutter, commander in chief of the Enlisted Man's Empire... It all happens at once—and five minutes later, more assassins attack Wayson Harris as he prepares for a summit with delegates of Olympus Kri. With Harris missing and their most deadly enemies on the loose, the remaining officers of the Empire must uncover a plot to overthrow their government while preparing for war... |
| zb50 clone: Clone #4 David Schulner, 2013-02-13 Luke Taylor is about to be a father any day now, but who is his father? The scientist behind the cloning project is the most wanted man in America, but where is he? And what will the rebels do when they get their hands on him? |
| zb50 clone: The Clone Sedition Steven L. Kent, 2012-10-30 Earth, A.D. 2519. Less than a year has passed since the clone military of the Enlisted Man’s Empire toppled the government of the Unified Authority. Now the clones rule Earth, but a new enemy has emerged—and set off civil war… Formerly trained to fight for the U.A., clone Marine Wayson Harris had led the Enlisted Man’s Empire invasion of Earth and wrested control away from the old regime. He’s now ready to do what it takes to ensure the new balance of power isn’t jeopardized. When a trio of religious fanatics from Mars attempts to attack Harris, he fears there is more unrest among the colony’s residents. Hoping to stave off an uprising, he leads a troop of Marines to Mars. But once there, they learn the situation is much graver than they first feared. The red planet’s refugees have decided the clones are their number one enemy, and measures to eradicate them are underway. And when Harris is kidnapped and drugged, he discovers something disturbing about himself. He can be reprogrammed… |
| zb50 clone: Cloning of America John M. Carlo, 2016-01-22 When Nazi Germany surrendered in 1945, the world was jubilantand largely unaware of the technological capabilities that the Nazis had developed. Along with untold wealth and ambitions to create a master race, Nazi scientists had perfected human cloning, dispersing their facilities to remote islands that remained hidden after the end of the war. Many of the inner circle who escaped decided that victory in an open war was not achievablebut that it might be possible to conquer the United States in time and with patience. Over the years, the Nazis work to install their clones in the US government, hoping eventually to get one elected president. But forces are at work to prevent the Nazi clones from taking over the nation. When auto mechanic Jon Donner is shot by clones, he is accidentally injected with a special gel developed by the Nazis that gives him superhuman powers. With these new abilities, he and the Speaker of the House of Representatives conspire through numerous clandestine missions to try to keep the United States safe and free. In this science fiction thriller, as Nazi scientists work to install clones in the US government, one man inadvertently gains superhuman powers and works to stop their nefarious plans. |
| zb50 clone: Cyborg Pat McKissack, Fredrick McKissack, John Patrick McKissack, 2011 In the next Clone Codes installment, Houston, a cyborg, tells his story. Houston must fight to overcome the rules of his strange universe, where the government uses laws to torment its citizens, especially cyborgs. |
| zb50 clone: The Clones Gloria Skurzynski, 2007-05-03 Clones are supposed to be identical... aren't they? Corgan, hero of the Virtual War, has been living a blissful, if placid, life on the Isles of Hiva, his reward for winning the War with Sharla and Brig. But what he doesn't know is that Brig died soon after the War, and yet is not truly gone. Sharla had saved some of Brig's DNA and has created clone-twins with it. Corgan's world is disrupted when Sharla brings one of the clone-twins, Seabrig, to him to raise on the island, while she keeps the other, Brigand, with her in the Domed City. However, when circumstances force Sharla to bring Brigand to the island, they find that while the boys may look identical, their temperaments are not. Brigand is haughty, willful, power hungry, and despises Corgan because of his relationship with Sharla. And, as a result of the cloning process, both boys are growing at an astonishing rate. In what may or may not have been an accident with his clone-twin, Seabrig is badly injured and must be airlifted from the island to receive medical treatment in the Domed City. This leaves Corgan alone with an increasingly dangerous and unstable Brigand, who is now his size, and looking to get rid of Corgan once and for all. A gripping sequel to Virtual War that could be ripped straight from the headlines -- in eighty years.... |
| zb50 clone: SuperZero and the Clone Crisis Jane de Souza, 2016-10-26 With great trouble come great laughs! Everyone including BigaByte is in a lousy mood because someone’s stealing their laughs? Whaaa? Plus SuperZero’s mom has a bewildering surprise for him. Plus, plus, plus there is a cunning clone in school who is turning himself into everyone else and creating full-on chaos. (Pssst, that’s your cue, SuperZero. Do your thing!) |
| zb50 clone: The Clone Republic Steven L. Kent, 2006-03-28 Earth, 2508 A.D. Humans have spread across the six arms of the Milky Way Galaxy. The Unified Authority controls Earth’s colonies with an iron fist and a powerful military—a military made up almost entirely of clones… Private first-class Wayson Harris was raised in a U.A. orphanage among thousands of clones born and bred to be the ultimate soldiers. But Harris isn’t like the other Marines: he has a mind of his own. He figures he’s paying for that independent streak when his first assignment out of boot camp is the smallest Marine outpost in the whole U.A. When a rogue general surfaces, the remote desert world Harris thought was a dead-end posting becomes anything but. Fighting off the general’s raid gains Harris a promotion. But it also brings him to the attention of some unfriendly U.A. leaders. They have their own plans for the military—plans Harris disrupts by his very existence. For in an army of clones, the one unforgivable sin is to be different… |
| zb50 clone: The Clone Republic , 2006 |
| zb50 clone: Clone Designer Till Symon, 2019-07-01 2984 Eintausend Jahre später überwachen Großkonzerne den Genpool der Menschen, ihre Wiedergeburt, und Lebensverlängerung gibt es nur über Bonuspunkte. Doch es herrscht Konsummüdigkeit. In der größten Weltwirtschaftskrise züchten Allsa Unternehmer mit einem Gen Cocktail den Supermanager Castello heran, der jedoch entgleist und sich selbst zum Herrscher des Universums machen möchte. Sein Erfolgskonzept liegt in einem Geheimprojekt namens Multirecon Plus. Dafür verschwinden 100 Top Clone Designer auf mysteriöse Weise. Till Symon beschreibt in seinem Debüt Roman beängstigend nachvollziehbar den Kontrollwahn machtgieriger Geschäftemacher, wenn ihnen die technischen Möglichkeiten der Zukunft in die Hand gegeben werden. Mit skurrilen und witzigen Einlagen, in einer rapiden Handlung, lässt dieser Roman nicht nur nachdenklich werden, sondern auch schmunzeln. |
| zb50 clone: Clone #18 David Schulner, Aaron Ginsburg, Wade Mcintyre, 2014-07-02 How far would you go to protect your family? |
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