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| sambrook molecular biology book: The Condensed Protocols from Molecular Cloning Joseph Sambrook, David William Russell, 2006 The Condensed Protocols From Molecular Cloning: A Laboratory Manualis a single–volume adaptation of the three–volume third edition of Molecular Cloning: A Laboratory Manual.This condensed book contains only the step–by–step portions of the protocols, accompanied by selected appendices from the world's best–selling manual of molecular biology techniques. Each protocol is cross–referenced to the appropriate pages in the original manual. This affordable companion volume, designed for bench use, offers individual investigators the opportunity to have their own personal collection of short protocols from the essential Molecular Cloning. |
| sambrook molecular biology book: Molecular Cloning Joseph Sambrook, E. F. Fritsch, Tom Maniatis, 1989-01-01 |
| sambrook molecular biology book: Molecular Cloning Joseph Sambrook, 2003 |
| sambrook molecular biology book: Guidebook to Molecular Chaperones and Protein-Folding Catalysts Mary-Jane Gething, 1997-11-27 The precise shape of a protein is a crucial factor in its function. How do proteins become folded into the right conformation? Molecular chaperones and protein folding catalysts bind to developing polypeptides in the cytoplasm and ensure correct folding and transport. This Guidebook catalogues the latest information on nearly 200 of these molecules, including the important class of heat shock proteins; each entry is written by leading researchers in the field. |
| sambrook molecular biology book: Nonmammalian Genomic Analysis Eric Hon Cheong Lai, 1996-01-01 Offering detailed protocols for those needing to construct a variety of maps and isolate genes, this unique book is intended to popularize the new techniques of genome analysis derived from the Human Genome Project. The power of these new methods is often most striking when applied to problems outside of human genetics, particularly the nonmammalian systems on which many researchers focus. Many of these organisms are economically important and biologically rich. Nonmammalian Genomic Analysis: A Practical Guide covers the how to aspects of preparation, handling, cloning, and analysis of large DNA and the creation of chromosome and genome maps. This lab manual facilitates the transfer of these technologies to small low tech environments and allows them to be used by those with no background in genome mapping or large-fragment cloning. Like having a local expert, this collection provides procedures for anyone, anywhere, and allows the replication of others' success. Key Features * Includes detailed and clearly-written step-by-step protocols * Evinces expected results and offers trouble shooting advice * Provides techniques appropriate for small laboratories as well as those with limited resources * Covers a broad variety of cloning systems, including single copy vectors * Discusses a diverse range of organisms, from prokaryotes to eukaryotes, from single-celled organisms to highly complex organisms |
| sambrook molecular biology book: Genomes T.A. Brown, 2006-06-08 Covering molecular genetics from the basics through to genome expression and molecular phylogenetics, Genomes 3 is the latest edition of this pioneering textbook. Newly updated to incorporate the recent major advances, Genomes 3 is an invaluable companion for any undergraduate throughout their studies in molecular genetics. Following extensive reviewing, the new edition has been significantly restructured. The single chapter on genome anatomies has been expanded into three chapters to incorporate the latest sequencing achievements. An additional chapter on understanding genome expression has also been included, while the chapters on studying genomes have been brought to the front of the book to align it more closely to the practical reality of molecular genetics tuition. The end-of-chapter exercises have been overhauled and extended to give students and lecturers a much wider range of tests and challenges. Multiple choice questions have been included for the first time and an innovative figure test has been introduced to test readers' visual understanding. |
| sambrook molecular biology book: Molecular Biology Techniques Heather B. Miller, D. Scott Witherow, Sue Carson, 2011-10-18 This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - Project approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions |
| sambrook molecular biology book: Molecular Neuroscience Rusty Lansford, 2014 A wide variety of powerful molecular techniques have been applied to biology in recent decades, ranging from recombinant DNA technologies to state-of-the-art imaging methods. But the plethora of techniques available combined with the complexities of neurobiological systems can make it difficult for neuroscientists to select and carry out an experimental procedure to effectively address the question at hand. This laboratory manual serves as a comprehensive practical guide to molecular and cellular methods for neuroscientists. It consists of five major sections: Working with Cells, Working with DNA, Working with RNA, Gene Transfer, and Imaging. Each includes step-by-step protocols and discussions of basic and cutting-edge procedures for working in that area. Fundamental techniques include maintaining a sterile working environment, purifying and culturing neural cells, isolating and manipulating DNA and RNA, and understanding and using a microscope. Advanced topics include single-neuron isolation and analysis, in vivo gene delivery and imaging, optogenetics, RNA interference, transgenic technologies, high-throughput analysis of gene expression (e.g., RNA-Seq), and constructing and imaging fluorescent proteins. The manual includes protocols developed in the Advanced Techniques in Molecular Neuroscience course offered annually at Cold Spring Harbor Laboratory, as well as protocols drawn from its best-selling lab manuals. It is an essential resource for all neuroscientists, from graduate students upward, who seek to use molecular techniques to probe the complexities of the nervous system. |
| sambrook molecular biology book: Techniques in Molecular Systematics and Evolution Rob DeSalle, Gonzalo Giribet, Ward Wheeler, 2013-12-01 The amount of information that can be obtained by using molecular techniques in evolution, systematics and ecology has increased exponentially over the last ten years. The need for more rapid and efficient methods of data acquisition and analysis is growing accordingly. This manual presents some of the most important techniques for data acquisition developed over the last years. The choice and justification of data analysis techniques is also an important and critical aspect of modern phylogenetic and evolutionary analysis and so a considerable part of this volume addresses this important subject. The book is mainly written for students and researchers from evolutionary biology in search for methods to acquire data, but also from molecular biology who might be looking for information on how data are analyzed in an evolutionary context. To aid the user, information on web-located sites is included wherever possible. Approaches that will push the amount of information which systematics will gather in the |
| sambrook molecular biology book: PCR Technology Henry Erlich, 2015-12-31 This is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
| sambrook molecular biology book: Basic Techniques in Molecular Biology Stefan Surzycki, 2012-12-06 This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here. |
| sambrook molecular biology book: Guide to Research Techniques in Neuroscience Matt Carter, Jennifer C. Shieh, 2015-02-27 Neuroscience is, by definition, a multidisciplinary field: some scientists study genes and proteins at the molecular level while others study neural circuitry using electrophysiology and high-resolution optics. A single topic can be studied using techniques from genetics, imaging, biochemistry, or electrophysiology. Therefore, it can be daunting for young scientists or anyone new to neuroscience to learn how to read the primary literature and develop their own experiments. This volume addresses that gap, gathering multidisciplinary knowledge and providing tools for understanding the neuroscience techniques that are essential to the field, and allowing the reader to design experiments in a variety of neuroscience disciplines. - Written to provide a hands-on approach for graduate students, postdocs, or anyone new to the neurosciences - Techniques within one field are compared, allowing readers to select the best techniques for their own work - Includes key articles, books, and protocols for additional detailed study - Data analysis boxes in each chapter help with data interpretation and offer guidelines on how best to represent results - Walk-through boxes guide readers step-by-step through experiments |
| sambrook molecular biology book: Gene Biotechnology William Wu, Helen H. Zhang, Michael J. Welsh, Peter B. Kaufman, 2016-04-19 Covering state-of-the-art technologies and a broad range of practical applications, the Third Edition of Gene Biotechnology presents tools that researchers and students need to understand and apply today's biotechnology techniques. Many of the currently available books in molecular biology contain only protocol recipes, failing to explain the princ |
| sambrook molecular biology book: Guidebook to the Cytoskeletal and Motor Proteins Thomas Kreis, Ronald Vale, 1999 Since the first edition, many new and important discoveries have been made in this expanding area of research. This updated edition details all that is currently known about the proteins involved in the microfilament, microtubule and intermediate filament systems in cells and their roles intension resistance and motor function. Biochemists will find this book particularly useful as will physiologists and biologists in fields in which these proteins are involved, such as neuromuscular research. |
| sambrook molecular biology book: Methods of Molecular Analysis in the Life Sciences Andreas Hofmann, Anne Simon, Tanja Grkovic, Malcolm Jones, 2014-06-19 An accessible overview of the most popular and cutting-edge methods for studying the properties of molecules and their interactions. |
| sambrook molecular biology book: RNA Donald Charles Rio, 2011 So much has been learned about RNA in the past ten years that the ability to purify, analyze, and manipulate RNA molecules is now essential in all kinds of bioscience. Originating in thereee of the field's most prominent laboratories, this manual provieds the necessary background and strategies for approaching any RNA investigation, as well as detailed protocols and extensive tips adn troubleshoothing information. It is required reading for every research laboratory in the life sciences. |
| sambrook molecular biology book: CRISPR-Cas Jennifer A. Doudna, Prashant Mali, 2016 CRISPR/Cas-based techniques are revolutionizing the way geneticists and molecular biologists modify DNA sequences and modulate gene expression in cells and organisms. This laboratory manual presents step-by-step protocols for applying this cutting-edge technology to any system of interest. Contributors describe approaches for de. |
| sambrook molecular biology book: Chromosome Structure and Function Rudi Appels, J. Perry Gustafson, 2012-12-06 A Historical Perspective on the Study of Chromosome Structure and Function R. Appels Division of Plant Industry CSIRO P.O. Box 1600 A.C.T. AUSTRALIA Modern physical science gives us no model to explain the re duplication of the gene-string in each cell generation, or to ex plain the production of effective quantities of specific enzymes or other agents by specific genes. The precise pairing and inter change of segments by homologous gene-strings at meiosis also suggest novel physical properties of this form of matter. Stadler (1954) The very strong influence of reductionism in the history of understanding chromosome structure and function is evident in the above quotation from Stadler's 1954 paper, The gene. Earlyob servations on the constancy of the cytological appearance of chromo somes and their regular behaviour in cell division led to specula tion on their biological importance. As genetics became more refined in the early decades of the 20th century the genes-on-a string model of chromosomes developed and greater emphasis was placed on the further dissection of these structures. As a result, in the 1980's the reductionist approach is reaching a crest as extensive regions of the genetic material are being sequenced. |
| sambrook molecular biology book: Fundamental Molecular Biology Lizabeth A. Allison, 2011-10-18 Unique in in its focus on eukaryotic molecular biology, this textbook provides a distillation of the essential concepts of molecular biology, supported by current examples, experimental evidence, and boxes that address related diseases, methods, and techniques. End-of-chapter analytical questions are well designed and will enable students to apply the information they learned in the chapter. A supplementary website include self-tests for students, resources for instructors, as well as figures and animations for classroom use. |
| sambrook molecular biology book: Techniques in Genetic Engineering Isil Aksan Kurnaz, 2015-05-08 Although designed for undergraduates with an interest in molecular biology, biotechnology, and bioengineering, this book-Techniques in Genetic Engineering-IS NOT: a laboratory manual; nor is it a textbook on molecular biology or biochemistry. There is some basic information in the appendices about core concepts such as DNA, RNA, protein, genes, and |
| sambrook molecular biology book: Functional Nucleic Acids Detection in Food Safety Wentao Xu, 2016-09-08 This book focuses on the development and applications of functional nucleic acid-based detection methods in the context of food safety. Offering a comprehensive overview of nucleic acids detection method in food safety for professionals and members of the public interested in this area, the book is divided into two parts. Part I addresses the basic principle of nucleic acid detection, while Part II presents novel applications of detection methods in genetically modified organisms, the identification of dead-alive microorganisms, microbial diversity, heavy metal detection, gene toxicity and non-coding RNA identification. As such, it provides readers a wealth of knowledge on the use of nucleic acids as targets and media in food safety. It offers a valuable resource for clinicians and basic scientists in the areas of food science and microbiology, and for all those who are interested in the concrete applications of molecular biological techniques. p> |
| sambrook molecular biology book: Molecular Cloning Joseph Sambrook, 2001 |
| sambrook molecular biology book: Plant Molecular Biology — A Laboratory Manual Melody S. Clark, 2013-11-27 The aim of this manual is to encompass a broad range of the latest plant molecular biology techniques. However, it is acknowledged that any manual will be read (and hopefully) used by a wide range of people with different levels of experience. Hence the remit of the manual was widened to include a full range of basic molecular tech niques, so that novices do not have to consult several texts to enable the execution of each major experiment. The manual is divided into three main parts: Part I: Basic Molecular Techniques The raison d'etre behind this part is to provide a background knowledge of molecular techniques, but also to reduce duplication in later chapters (this is particularly true of the methods contained in Chap. 1). All authors provided very detailed methods and often forgot that so me of these would be covered earlier. A particular favourite was DNA extraction methods, wherc everyone managed to provide a slightly different variant! My view was that it is far less confusing for the reader to be presented with one standard protocol and accom panying troubleshooting tips, than to read a different version in each chapter. In this way the basic techniques are addressed more in depth (and my apologies to all authors for judicious use of the delete key!). RNA methodology is covered in Chapter 3. This proceeds from the fundamentals of extraction, northern blotting etc. , to cDNA libraries. |
| sambrook molecular biology book: Who Has Seen a Blood Sugar? Frank Davidoff, Susan Deutsch, Kathleen L. Egan, Jack Ende, 1996 Medicine is a powerful way of shaping the invisible world, and in that power lie both medicine's benefit and its limitation. Teaching and learning medicine are unusually complex, and present subtle changes. The 41 essays in Who Has Seen a Blood Sugar? are not directly concerned with teaching methods and techniques. Rather, each takes as its starting point some particularly critical or problematic element of medical education, develops new and different ways of thinking about it, and explores better ways to approach it. Many of these insights come from sources outside medicine, from fields as diverse as mathematics, linguistics, poetry, music, philosophy, and literature. All essays are referenced, pointing readers to additional sources of background material and detail. In fact, the nearly 250 references may be of special interest and use to readers. |
| sambrook molecular biology book: Biopharmaceutical Drug Design and Development Susanna Wu-Pong, Yongyut Rojanasakul, 1999 A unique comprehensive survey and review of the status of the rapidly increasing array of biopharmaceuticals derived from the molecular biology approaches now so widely available. Designed as an introduction for the novice-or even the professional already acquainted with molecular therapeutics-who wishes to become familiar with both the basics and the range of biopharmaceutical initiatives now underway, this informative book illuminates and explains macromolecular and biological drug design, as well as the development, delivery, clinical trials, and government regulation of the new therapeutics. Its eminently practical approach takes the reader from basic biotechnology, through drug conception and design, to state-of-the-art clinical application of both investigational and currently approved biologicals. Biopharmaceutical Drug Design and Development will help medicinal chemists, molecular modelers, pharmacologists, pharmaceutical scientists, and clinical scientists involved in pharmaceutical biotechnology to develop the new therapeutic agents that lie so close ahead. |
| sambrook molecular biology book: Gene Cloning and Manipulation Christopher Howe, 2007-07-12 Updated to reflect advances in the field, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams. |
| sambrook molecular biology book: DNA Microarrays David Bowtell, Joseph Sambrook, 2003 DNA microarray technology is a new and powerful means to analyze genomes and characterize patterns of gene expression. Its applications are widespread across the many fields of plant and animal biological and biomedical research. This manual, designed to extend and to complement the information in the best–selling Molecular Cloning,is a synthesis of the expertise and experience of more than 30 contributors—all innovators in a fast–moving field. DNA Microarraysprovides authoritative, detailed instruction on the design, construction, and applications of microarrays, as well as comprehensive descriptions of the software tools and strategies required for analysis of images and data. |
| sambrook molecular biology book: Comparative Animal Biochemistry Klaus Urich, 2013-04-17 tribute greatly to understanding the origins of The plan for this book goes back almost 20 years. Already, at that time, it was possible to recognize organisms. an extraordinary variation in metabolites and To provide the biochemist with a ready over processes superimposed upon the basic biochem view of the structural diversity of animals, the book includes a simplified version of animal sys ical system of animals. Each species, each indi tematics; for further information on the classifica vidual, in fact each type of cell of the multicellu lar organism possesses its own biochemical char tion, structure and life of particular animal spe acter, and this molecular variety, its biological sig cies, the reader should consult the relevant text nificance, and its evolutionary development books. It is assumed that the zoologist reader has throw up many interesting questions. The com a basic knowledge of biochemistry; important general biochemical facts are in any case given for parative approach that has been so productive at many of the subjects covered. the higher levels of complexity of morphology and physiology can also be used to great effect at I had already completed several chapters of the molecular level. this book by the beginning of the 1970s. |
| sambrook molecular biology book: Breeding For Ornamentals: Classical and Molecular Approaches A. Vainstein, 2013-04-17 In this book we bring together the most up-to-date information on developments, both basic and applied, that already have or are expected to impact the field of ornamental breeding. These include classical and molecular techniques, traditional and high-throughput approaches and future trends. Since not only professional scientists, but also thousands of future scientists/students as well as amateur breeders around the world contribute heavily to the field of ornamental breeding, an introductory section dealing with the basics of molecular and classical genetics and the evolution of floral diversity is included. This should enable the reader to bridge the gap between traditional and molecular genetics. Classical approaches to the creation/selection of genetic variability, including mutation and tissue culture-aided breeding, are presented. Processes affecting ornamental and agronomic traits at the molecular level are delineated, along with an in-depth analysis of developments in the protection of intellectual property rights. The thoughts and strategies of molecular and classical geneticists, which are not always complementary or even compatible, are presented side by side in this book, and will serve to spark the imaginations of breeders as well as students entering the exciting world of state-of-the-art ornamentals. |
| sambrook molecular biology book: Mouse Genetics , 2001 |
| sambrook molecular biology book: Plant Biotechnology and Genetics C. Neal Stewart, Jr., 2012-12-13 Designed to inform and inspire the next generation of plant biotechnologists Plant Biotechnology and Genetics explores contemporary techniques and applications of plant biotechnology, illustrating the tremendous potential this technology has to change our world by improving the food supply. As an introductory text, its focus is on basic science and processes. It guides students from plant biology and genetics to breeding to principles and applications of plant biotechnology. Next, the text examines the critical issues of patents and intellectual property and then tackles the many controversies and consumer concerns over transgenic plants. The final chapter of the book provides an expert forecast of the future of plant biotechnology. Each chapter has been written by one or more leading practitioners in the field and then carefully edited to ensure thoroughness and consistency. The chapters are organized so that each one progressively builds upon the previous chapters. Questions set forth in each chapter help students deepen their understanding and facilitate classroom discussions. Inspirational autobiographical essays, written by pioneers and eminent scientists in the field today, are interspersed throughout the text. Authors explain how they became involved in the field and offer a personal perspective on their contributions and the future of the field. The text's accompanying CD-ROM offers full-color figures that can be used in classroom presentations with other teaching aids available online. This text is recommended for junior- and senior-level courses in plant biotechnology or plant genetics and for courses devoted to special topics at both the undergraduate and graduate levels. It is also an ideal reference for practitioners. |
| sambrook molecular biology book: Real-time PCR M Dorak, 2007-01-24 With a variety of detection chemistries, an increasing number of platforms, multiple choices for analytical methods and the jargon emerging along with these developments, real-time PCR is facing the risk of becoming an intimidating method, especially for beginners. Real-time PCR provides the basics, explains how they are exploited to run a real-time PCR assay, how the assays are run and where these assays are informative in real life. It addresses the most practical aspects of the techniques with the emphasis on 'how to do it in the laboratory'. Keeping with the spirit of the Advanced Methods Series, most chapters provide an experimental protocol as an example of a specific assay. |
| sambrook molecular biology book: Cell Biology Julio E. Celis, 1998 V. 1: cell and tissue culture and associated techniques; Primary cultures from embyonic and newborn tissues; Culture of specific cell types; Cell separation techniques; Model systems to study differentiation; cell cycle analysis; Assays of tumorigenicity, invasion, and others; Cytotoxic and cell growth assays; Senescence and apoptosis; Electrophysiological methods; Histocultures and organ cultures; Other cell types and organisms; Viruses; Appendices; v. 2: Organelles and cellular structures; Assays; Antibodies; Immunocytochemistry; Vital staining of cells; v. 3: Light microscopy and contrast generation; Electron microscopy; Intracellular measurments; Cytogenetics and in situ hybridization; transgenic and gene knockouts; v. 4: Transfer of macromelcules and small molecules; Expression systems; Differential gene expression; Proteins; Appendix; List of suppliers; Subject index. |
| sambrook molecular biology book: Molecular Pharmacology John Dickenson, Fiona Freeman, Chris Lloyd Mills, Christian Thode, Shiva Sivasubramaniam, 2013-01-22 This textbook provides a fresh, comprehensive and accessible introduction to the rapidly expanding field of molecular pharmacology. Adopting a drug target-based, rather than the traditional organ/system based, approach this innovative guide reflects the current advances and research trend towards molecular based drug design, derived from a detailed understanding of chemical responses in the body. Drugs are then tailored to fit a treatment profile, rather than the traditional method of ‘trial and error’ drug discovery which focuses on testing chemicals on animals or cell cultures and matching their effects to treatments. Providing an invaluable resource for advanced under-graduate and MSc/PhD students, new researchers to the field and practitioners for continuing professional development, Molecular Pharmacology explores; recent advances and developments in the four major human drug target families (G-protein coupled receptors, ion channels, nuclear receptors and transporters), cloning of drug targets, transgenic animal technology, gene therapy, pharmacogenomics and looks at the role of calcium in the cell. Current - focuses on cutting edge techniques and approaches, including new methods to quantify biological activities in different systems and ways to interpret and understand pharmacological data. Cutting Edge - highlights advances in pharmacogenomics and explores how an individual’s genetic makeup influences their response to therapeutic drugs and the potential for harmful side effects. Applied - includes numerous, real-world examples and a detailed case-study based chapter which looks at current and possible future treatment strategies for cystic fibrosis. This case study considers the relative merits of both drug therapy for specific classes of mutation and gene therapy to correct the underlying defect. Accessible - contains a comprehensive glossary, suggestions for further reading at the end of each chapter and an associated website that provides a complete set of figures from within the book. |
| sambrook molecular biology book: Plant Biotechnology H. S. Chawla, 2003 Basics; Laboratory organization; Sterilization techniques; Nutrition medium; Choice of the explant; Plant tissue culture; Seed culture; Micropropagation- meristem culture; Micropropagation- axillary bud proliferation; Micropropagation- adventitious regeneration; Micropropagation- organogenesis; Micropropagation- embryogenesis; Cell suspension; Secondary metabolite production in a cell suspension culture; Anther culture; Protoplast isolation and fusion; Biotechnology; SDS-PAGE electrophoresis of proteins; Isolation of DNA from plant tissues; Isolation an purification of plasmid DNA; Restriction enzyme digestion of DNA; Agarose gel electrophoresis; Preparation of competent cells, transformation of E. coil with plasmid DNA and ligation of insert DNA to a vector; Agrobacterium-mediated gene transfer; Biolistic method of transformation in plants; In vitro amplification of DNA by PCR: detection of transgenes; RAPD analysis; Microsatellite marker analysis; Southerm biotting; Southerm hybridization. |
| sambrook molecular biology book: Biochemistry of Cell Membranes S. Papa, J.M. Tager, 2012-12-06 This book consists of a series of reviews on selected topics within the rapidly and vastly expanding field of membrane biology. Its aim is to highlight the most significant and important advances that have been made in recent years in understanding the structure, dynamics and functions of cell membranes. Areas covered in this monograph include: • Signal Transduction • Membrane Traffic: Protein and Lipids • Bioenergetics: Energy Transfer and Membrane Transport • Cellular Ion Homeostasis • Growth Factors and Adhesion Molecules • Structural Analysis of Membrane Proteins • Membranes and Disease. Biochemistry of Cell Membranes should serve as a benchmark for indicating the most important lines for future research in these areas. |
| sambrook molecular biology book: Introduction to Pharmaceutical Biotechnology, Volume 1 (Second Edition): Basic Techniques and Concepts , 2024-11 |
| sambrook molecular biology book: Molecular Biology And Biotechnology J M Walker, E B Gingold, 1999 |
| sambrook molecular biology book: PCR Protocols John M.S. Bartlett, David Stirling, 2003-08 Drawing on the proven qualities of the much praised and widely used first edition, John M. S. Bartlett and David Stirling have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These successful methods include real-time PCR, SNP analysis, nested PCR, direct PCR, and long-range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. In situ PCR methods and their application in parallel with other methods, such as immunohistochemistry, are also included. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on troubleshooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results. Cutting-edge and highly practical, PCR Protocols, Second Edition provides both novice and experienced investigators with an up-to-date compendium of powerful PCR methods for easy reference and consultation in the day-to-day performance of PCR-based experimentation, one that will enhance understanding of PCR, satisfy current needs, and point to powerful future applications. |
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