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| principles of fluorescence spectroscopy lakowicz free download: Principles of Fluorescence Spectroscopy Joseph R. Lakowicz, 2007-12-05 The third edition of this established classic text reference builds upon the strengths of its very popular predecessors. Organized as a broadly useful textbook Principles of Fluorescence Spectroscopy, 3rd edition maintains its emphasis on basics, while updating the examples to include recent results from the scientific literature. The third edition includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes and radiative decay engineering. Includes a link to Springer Extras to download files reproducing all book artwork, for easy use in lecture slides. This is an essential volume for students, researchers, and industry professionals in biophysics, biochemistry, biotechnology, bioengineering, biology and medicine. |
| principles of fluorescence spectroscopy lakowicz free download: Fluorescence Studies of Polymer Containing Systems Karel Procházka, 2016-02-03 This volume describes the application of fluorescence spectroscopy in polymer research. The first chapters outline the basic principles of the conformational and dynamic behavior of polymers and review the problems of polymer self-assembly. Subsequent chapters introduce the theoretical principles of advanced fluorescence methods and typical examples of their application in polymer science. The book closes with several reviews of various fluorescence applications for studying specific aspects of polymer-solution behavior. It is a useful resource for polymer scientists and experts in fluorescence spectroscopy alike, facilitating their communication and cooperation. |
| principles of fluorescence spectroscopy lakowicz free download: Principles and Applications of Fluorescence Spectroscopy Jihad Rene Albani, 2007-10-08 Fluorescence spectroscopy is an important investigational tool in many areas of analytical science, due to its extremely high sensitivity and selectivity. With many uses across a broad range of chemical, biochemical and medical research, it has become an essential investigational technique allowing detailed, real-time observation of the structure and dynamics of intact biological systems with extremely high resolution. It is particularly heavily used in the pharmaceutical industry where it has almost completely replaced radiochemical labelling. Principles and Applications of Fluorescence Spectroscopy gives the student and new user the essential information to help them to understand and use the technique confidently in their research. By integrating the treatment of absorption and fluorescence, the student is shown how fluorescence phenomena arise and how these can be used to probe a range of analytical problems. A key element of the book is the inclusion of practical laboratory experiments that illustrate the fundamental points and applications of the technique. |
| principles of fluorescence spectroscopy lakowicz free download: New Trends in Fluorescence Spectroscopy Bernard Valeur, Jean-Claude Brochon, 2012-12-06 Fluorescence is more and more widely used as a tool of investigation, analysis, control and diagnosis in many fields relevant to physical, chemical, biological and medical sciences. New technologies con tinuously emerge thanks to the progress in the design of light sources (e.g. laser diodes), detectors (3D, 4D) and compact ultrafast elec tronic devices. In particular, much progress has been made in time resolved fluorescence microscopy (FUM: Fluorescence Lifetime Imaging Microscopy; FCS: Fluorescence Correlation Spectroscopy). Furthermore, the sensitivity now allows one to detect a single mole cule in the restricted field of a confocal microscope, which actually offers the possibility to study phenomena at a molecular level. The development of new fluorescent probes is still a necessity. In particular, the growing use of lasers implies high resistance to photo degradation. Fluorescence emission at long wavelengths is also a distinct advantage. Furthermore, in vivo inclusion of new fluorescent aromatic residues in proteins offer new potentialities in biology. of ions and molecules is Fluorescence-based selective detection still the object of special attention. Considerable effort is being made in the design of supramolecular systems in which the recognition event is converted into a fluorescence signal easily detected. New fluorescent sensors for clinical diagnosis and detection of pollutants in atmosphere and water are extensively developed. All these developments justify the regular publication of books giving the state-of-the-art of the methods and applications of fluo rescence spectroscopy. |
| principles of fluorescence spectroscopy lakowicz free download: Introduction to Fluorescence Spectroscopy Ashutosh Sharma, Stephen G. Schulman, 1999-05-21 This book introduces the uninitiated reader to the growing body of analytical methods based on molecular fluorescence. Geared to practitioners with no particular training or exposure to the field, it highlights fluorescence spectroscopy's tremendous appeal in present-day pharmaceutical, biomedical, and environmental analysis. |
| principles of fluorescence spectroscopy lakowicz free download: Applied Fluorescence in Chemistry, Biology and Medicine Wolfgang Rettig, 1999 This interdisciplinary book gives a comprehensive survey of the state-of-the-art: from applications and trends in fluorescence techniques in science to medicine and engineering. Written for practitioners and researchers in industry and academia, it covers fields like environmental and materials science, biology, medicine, physics and chemistry. Moreover, it reports on such new and breathtaking methods as ultra-fast time-resolved or single molecule spectroscopy, gives examples of applications in the fields of electroluminescent polymers, visualization of membrane potentials in neurons and fluorescence imaging of the brain. |
| principles of fluorescence spectroscopy lakowicz free download: Introduction to Fluorescence David M. Jameson, 2025-03-26 The phenomenon known as ‘fluorescence’ is now widely used in the chemical, physical and life sciences largely due to the development of highly sophisticated fluorescent probe chemistries and the commercial availability of these probes as well as the development of novel microscopy approaches. This Second Edition of Introduction to Fluorescence helps readers acquire a thorough understanding of basic fluorescence theory and practice. It describes the general principles in a direct way and uses examples from a variety of disciplines to demonstrate them. Since the First Edition, significant advances in the field have appeared. For example, phasors, both lifetime and spectral phasors, have become very popular, and so a new chapter dedicated to this topic has been added in this edition. Furthermore, significant advances have been made in fluorescence microscopy methods, including super-resolution and single-molecule techniques. In color throughout, the book takes readers through the history of important discoveries to the most current advances. It introduces the fundamentals of the fluorescence phenomenon and gives detailed examples of fluorescence applications in the molecular life sciences, including biochemistry, biophysics, clinical chemistry and diagnostics, pharmaceutical science, and cell and molecular biology. The author presents the basic theories underlying the applications and offers in-depth information on practical aspects. Numerous references are given in each chapter, along with a list of particularly important references at the end of each chapter. The text incorporates more than 340 figures that clearly illustrate the concepts and gives the chemical structures of the most widely used fluorescent molecules. In addition, Chapter 13, the Appendix, provides a Rogue’s Gallery of the most common errors and pitfalls to avoid. |
| principles of fluorescence spectroscopy lakowicz free download: Electron Crystallography Xiaodong Zou, Sven Hovmöller, Peter Oleynikov, 2011-08-18 In the modern world of ever smaller devices and nanotechnology, electron crystallography emerges as the most important method capable of determining the structure of minute objects down to the size of individual atoms. Crystals of only a few millionths of a millimetre are studied. This is the first textbook explaining how this is done. Great attention is given to symmetry in crystals and how it manifests itself in electron microscopy and electron diffraction, and how this symmetry can be determined and taken advantage of in achieving improved electron microscopy images and solving crystal structures from electron diffraction patterns. Theory and practice are combined; experimental images, diffraction patterns, formulae and numerical data are discussed in parallel, giving the reader a complete understanding of what goes on inside the black boxes of computer programs. This up-to-date textbook contains the newest techniques in electron crystallography, including detailed descriptions and explanations of the recent remarkable successes in determining the very complex structures of zeolites and intermetallics. The controversial issue of whether there is phase information present in electron micrsocopy images or not is also resolved once and for all. The extensive appendices include computer labs which have been used at various courses at Stockholm University and international schools in electron crystallography, with applications to the textbook. Students can download image processing programs and follow these lab instructions to get a hands-on experience of electron crystallography. |
| principles of fluorescence spectroscopy lakowicz free download: Time-correlated single photon counting Desmond O'Connor, 2012-12-02 Time-correlated Single Photon Counting has been written in the hope that by relating the authors' experiences with a variety of different single photon counting systems, they may provide a useful service to users and potential users of this formidably sensitive technique. Of all the techniques available to obtain information on the rates of depopulation of excited electronic singlet states of molecular species, monitoring of fluorescence provides, in principle, the simplest and most direct measure of concentration. This volume comprises eight chapters, with the first focusing on the time dependence and applications of fluorescence. Succeeding chapters go on to discuss basic principles of the single photon counting lifetime measurement; light sources; photomultipliers; electronics; data analysis; nanosecond time-resolved emission spectroscopy; time dependence of fluorescence anisotropy. This book will be of interest to practitioners in the field of chemistry. |
| principles of fluorescence spectroscopy lakowicz free download: Fluorescent Analogs of Biomolecular Building Blocks Marcus Wilhelmsson, Yitzhak Tor, 2016-04-04 Fluorescent Analogs of Biomolecular Building Blocks focuses on the design of fluorescent probes for the four major families of macromolecular building blocks. Compiling the expertise of multiple authors, this book moves from introductory chapters to an exploration of the design, synthesis, and implementation of new fluorescent analogues of biomolecular building blocks, including examples of small-molecule fluorophores and sensors that are part of biomolecular assemblies. |
| principles of fluorescence spectroscopy lakowicz free download: Fluorescence Microscopy Ulrich Kubitscheck, 2017-03-27 Zu dem Thema gibt es viele Publikationen, die von Experten für Experten geschrieben wurden. Dieses Buch wendet sich insbesondere an Studenten höherer Semester und Forscher, denen das Hintergrundwissen der Physik fehlt, um neuartige Verfahren der Fluoreszenzmikroskopie zu verstehen. Die zweite Auflage wartet mit neuen Kapiteln und einer erweiterten Einführung auf. Der Schwerpunkt liegt auf der hochauflösenden und Einzelmolekül-Mikroskopie. Jedes Kapitel wurde von einem anerkannten Experten des Fachgebiets geschrieben und sorgfältig überarbeitet, um so die Entwicklungen der letzten Jahre wiederzugeben. |
| principles of fluorescence spectroscopy lakowicz free download: Optical Measurements for Scientists and Engineers Arthur McClelland, Max Mankin, 2018-04-19 An accessible, introductory text explaining how to select, set up and use optical spectroscopy and optical microscopy techniques. |
| principles of fluorescence spectroscopy lakowicz free download: Fluorescence Spectroscopy and Microscopy Yves Engelborghs, Antonie J.W.G. Visser, 2016-08-23 Reflecting the expanding field’s need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject. The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS). Written as a part of the popular Methods in Molecular Biology series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results. Comprehensive and practical, Fluorescence Spectroscopy and Microscopy: Methods and Protocols aims to guide both ‘novice’ and established scientists toward furthering their research with these invaluable techniques. |
| principles of fluorescence spectroscopy lakowicz free download: Fluorescence Spectroscopy , 2011-09-06 Fluorescence spectroscopy is a type of electromagnetic spectroscopy, using a beam of light, which analyzes fluorescence from a sample. Given its extremely high sensitivity and selectivity, it is an important investigational tool in many areas including material sciences, analytical sciences, and across a broad range of chemical, biochemical and medical research. It has become an essential investigational technique allowing detailed, real-time observation of the structure and dynamics of intact biological systems. The pharmaceutical industry uses it heavily and it has become a dominating technique in biochemistry and molecular genetics. - Keeps MIE buyers and online subscribers up-to-date with the latest research with this highly used technique - Provides tried and tested techniques which eliminate searching through many different sources |
| principles of fluorescence spectroscopy lakowicz free download: Biomolecular NMR Spectroscopy Jeremy N. S. Evans, 1995 The technique of nuclear magnetic resonance (NMR) spectroscopy is an important tool in biochemistry and biophysics for the understanding of the structure and ultimately, the function of biomolecules. This textbook explains the salient features of biological NMR spectroscopy to undergraduates and postgraduates taking courses in NMR, biological NMR, physical biochemistry, and biophysics. Unlike other books in the general field of NMR (except the advanced treatises), the approach here is tointroduce and make use of quantum mechanical product operators as well as the classical vector method of explaining the bewildering array of pulse sequences available today. The book covers two- dimensional, three- dimensional, and four- dimensional NMR and their application to protein and DNA structure determination. A unique feature is the coverage of the biological aspects of solid- state NMR spectroscopy. The author provides many selected examples from the research literature, illustratingthe applications of NMR spectroscopy to biological proteins. |
| principles of fluorescence spectroscopy lakowicz free download: Molecular Beacons Chaoyong James Yang, Weihong Tan, 2013-08-15 Molecular Beacons explains working principle of molecular beacons, discusses their design, synthesis, purification and characterization, explores their thermodynamic and kinetic properties, and more importantly, reviews their in vivo and in vitro applications with the emphasis on the design and modification of molecular beacons for in vivo mRNA imaging applications. This book is designed to bring together in a single resource an organized and comprehensive view of molecular beacons and will be a valuable resource for academic, clinical and industrial scientists and graduate students who may consider exploring molecular beacons in their research or practice. Chaoyong James Yang is the Lu Jiaxi Professor of Chemistry at Xiamen University, China. Weihong Tan is a Distinguished Professor of Chemistry and Biomedical Engineering at Hunan University, China and also a University of Florida Distinguished Professor and V. T. and Louis Jackson Professor of Chemistry at the University of Florida, USA. |
| principles of fluorescence spectroscopy lakowicz free download: Applied Nanophotonics Sergey V. Gaponenko, Hilmi Volkan Demir, 2018-11-22 With full color throughout, this unique text provides an accessible yet rigorous introduction to the basic principles, technology, and applications of nanophotonics. It explains key physical concepts such as quantum confinement in semiconductors, light confinement in metal and dielectric nanostructures, and wave coupling in nanostructures, and describes how they can be applied in lighting sources, lasers, photonic circuitry, and photovoltaic systems. Readers will gain an intuitive insight into the commercial implementation of nanophotonic components, in both current and potential future devices, as well as challenges facing the field. The fundamentals of semiconductor optics, optical material properties, and light propagation are included, and new and emerging fields such as colloidal photonics, Si-based photonics, nanoplasmonics, and bioinspired photonics are all discussed. This is the 'go-to' guide for graduate students and researchers in electrical engineering who are interested in nanophotonics, and students taking nanophotonics courses. |
| principles of fluorescence spectroscopy lakowicz free download: Molecular Fluorescence Bernard Valeur, Mário Nuno Berberan-Santos, 2013-03-25 Molecular Fluorescence This second edition of the well-established bestseller is completely updated and revised with approximately 30 % additional material, including two new chapters on applications, which has seen the most significant developments. The comprehensive overview written at an introductory level covers fundamental aspects, principles of instrumentation and practical applications, while providing many valuable tips. For photochemists and photophysicists, physical chemists, molecular physicists, biophysicists, biochemists and biologists, lecturers and students of chemistry, physics, and biology. |
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| principles of fluorescence spectroscopy lakowicz free download: Molecular Spectroscopy Jeanne L. McHale, 2017-07-06 This textbook offers an introduction to the foundations of spectroscopic methods and provides a bridge between basic concepts and experimental applications in fields as diverse as materials science, biology, solar energy conversion, and environmental science. The author emphasizes the use of time-dependent theory to link the spectral response in the frequency domain to the behavior of molecules in the time domain, strengthened by two brand new chapters on nonlinear optical spectroscopy and time-resolved spectroscopy. Theoretical underpinnings are presented to the extent necessary for readers to understand how to apply spectroscopic tools to their own interests. |
| principles of fluorescence spectroscopy lakowicz free download: Metal-Enhanced Fluorescence Chris D. Geddes, 2010-06-22 Discover how metal-enhanced fluorescence is changing traditional concepts of fluorescence This book collects and analyzes all the current trends, opinions, and emerging hot topics in the field of metal-enhanced fluorescence (MEF). Readers learn how this emerging technology enhances the utility of current fluorescence-based approaches. For example, MEF can be used to better detect and track specific molecules that may be present in very low quantities in either clinical samples or biological systems. Author Chris Geddes, a noted pioneer in the field, not only explains the fundamentals of metal-enhanced fluorescence, but also the significance of all the most recent findings and models in the field. Metal-enhanced fluorescence refers to the use of metal colloids and nanoscale metallic particles in fluorescence systems. It offers researchers the opportunity to modify the basic properties of fluorophores in both near- and far-field fluorescence formats. Benefits of metal-enhanced fluorescence compared to traditional fluorescence include: Increased efficiency of fluorescence emission Increased detection sensitivity Protect against fluorophore photobleaching Applicability to almost any molecule, including both intrinsic and extrinsic chromophores Following a discussion of the principles and fundamentals, the author examines the process and applications of metal-enhanced fluorescence. Throughout the book, references lead to the primary literature, facilitating in-depth investigations into particular topics. Guiding readers from the basics to state-of-the-technology applications, this book is recommended for all chemists, physicists, and biomedical engineers working in the field of fluorescence. |
| principles of fluorescence spectroscopy lakowicz free download: Topics in Fluorescence Spectroscopy Joseph R. Lakowicz, 1991 This third volume details the applications of fluorescence spectroscopy in biochemistry and biophysics. |
| principles of fluorescence spectroscopy lakowicz free download: Fluorescence and Phosphorescence Spectroscopy Stephen G Schulman, 2017-05-17 Fluorescence and Phosphorescence Spectroscopy: Physicochemical Principles and Practice deals with the physicochemical principles and applications of fluorescence and phosphorescence spectroscopy in experimental biology and chemistry. Topics covered include the absorption of light by molecules; instrumentation for the measurement of fluorescence and phosphorescence; solvent and acidity effects on electronic spectra; and polarization of fluorescence and phosphorescence. Comprised of four chapters, this book begins with a discussion on photophysical processes in isolated molecules and molecules in solution, paying particular attention to thermal equilibration of electronically excited molecules, phototautomerism, and coordination by metal ions. The next chapter describes the instrumentation for measuring fluorescence and phosphorescence, which consists essentially of a light source to electronically excite the sample; a monochromator to separate the light of desired energy from the source; a sample compartment; a second monochromator to isolate the sample's fluorescence energy from the excitation energy; a photodetector to translate the fluorescent light into an electrical signal; and a readout system such as a galvanometer or a recorder, coupled with an amplifier to determine the intensity of fluorescent light that is emitted. The final chapter is devoted to various applications of fluorescence and phosphorescence spectroscopy, including the analysis of organic and inorganic compounds. This monograph is written primarily for analytical chemists and biological scientists. |
| principles of fluorescence spectroscopy lakowicz free download: Spectrophotometry & Spectrofluorimetry D. A. Harris, C. L. Bashford, David A. Harris, 1987 Using this book biochemists can determine how spectrophotometry can contribute to laboratory analyses. Emphasis is placed on the capabilities and limitations of the instrument in use--how to select a machine, how to check if it is working satisfactorily, and what to do if it fails to produce the data expected. |
| principles of fluorescence spectroscopy lakowicz free download: Introduction to Fluorescence Sensing Alexander P. Demchenko, 2015-10-06 Fluorescence is the most popular technique in chemical and biological sensing and this book provides systematic knowledge of basic principles in the design of fluorescence sensing and imaging techniques together with critical analysis of recent developments. Its ultimate sensitivity, high temporal and spatial resolution and versatility enables high resolution imaging within living cells. It develops rapidly in the directions of constructing new molecular recognition units, new fluorescence reporters and in improving sensitivity of response, up to the detection of single molecules. Its application areas range from the control of industrial processes to environmental monitoring and clinical diagnostics. Being a guide for students and young researchers, it also addresses professionals involved in basic and applied research. Making a strong link between education, research and product development, this book discusses prospects for future progress. |
| principles of fluorescence spectroscopy lakowicz free download: Advanced Time-Correlated Single Photon Counting Techniques Wolfgang Becker, 2005-12-19 In 1984 Desmond O’Connor and David Phillips published their comprehensive book „Time-correlated Single Photon Counting“. At that time time-correlated s- gle photon counting, or TCSPC, was used primarily to record fluorescence decay functions of dye solutions in cuvettes. From the beginning, TCSPC was an am- ingly sensitive and accurate technique with excellent time-resolution. However, acquisition times were relatively slow due to the low repetition rate of the light sources and the limited speed of the electronics of the 70s and early 80s. Moreover, TCSPC was intrinsically one-dimensional, i.e. limited to the recording of the wa- form of a periodic light signal. Even with these limitations, it was a wonderful te- nique. More than 20 years have elapsed, and electronics and laser techniques have made impressive progress. The number of transistors on a single chip has approximately doubled every 18 months, resulting in a more than 1,000-fold increase in compl- ity and speed. The repetition rate and power of pulsed light sources have increased by about the same factor. |
| principles of fluorescence spectroscopy lakowicz free download: Handbook of Neurochemistry Abel Lajtha, 1982 |
| principles of fluorescence spectroscopy lakowicz free download: Analytical Techniques in the Pharmaceutical Sciences Anette Müllertz, Yvonne Perrie, Thomas Rades, 2016-08-30 The aim of this book is to present a range of analytical methods that can be used in formulation design and development and focus on how these systems can be applied to understand formulation components and the dosage form these build. To effectively design and exploit drug delivery systems, the underlying characteristic of a dosage form must be understood--from the characteristics of the individual formulation components, to how they act and interact within the formulation, and finally, to how this formulation responds in different biological environments. To achieve this, there is a wide range of analytical techniques that can be adopted to understand and elucidate the mechanics of drug delivery and drug formulation. Such methods include e.g. spectroscopic analysis, diffractometric analysis, thermal investigations, surface analytical techniques, particle size analysis, rheological techniques, methods to characterize drug stability and release, and biological analysis in appropriate cell and animal models. Whilst each of these methods can encompass a full research area in their own right, formulation scientists must be able to effectively apply these methods to the delivery system they are considering. The information in this book is designed to support researchers in their ability to fully characterize and analyze a range of delivery systems, using an appropriate selection of analytical techniques. Due to its consideration of regulatory approval, this book will also be suitable for industrial researchers both at early stage up to pre-clinical research. |
| principles of fluorescence spectroscopy lakowicz free download: Spectroscopic Analyses Eram Sharmin, Fahmina Zafar, 2017-12-06 The book presents developments and applications of these methods, such as NMR, mass, and others, including their applications in pharmaceutical and biomedical analyses. The book is divided into two sections. The first section covers spectroscopic methods, their applications, and their significance as characterization tools; the second section is dedicated to the applications of spectrophotometric methods in pharmaceutical and biomedical analyses. This book would be useful for students, scholars, and scientists engaged in synthesis, analyses, and applications of materials/polymers. |
| principles of fluorescence spectroscopy lakowicz free download: Physical Chemistry of Macromolecules S. F. Sun, 2004-01-28 Integrating coverage of polymers and biological macromolecules into a single text, Physical Chemistry of Macromolecules is carefully structured to provide a clear and consistent resource for beginners and professionals alike. The basic knowledge of both biophysical and physical polymer chemistry is covered, along with important terms, basic structural properties and relationships. This book includes end of chapter problems and references, and also: Enables users to improve basic knowledge of biophysical chemistry and physical polymer chemistry. Explores fully the principles of macromolecular chemistry, methods for determining molecular weight and configuration of molecules, the structure of macromolecules, and their separations. |
| principles of fluorescence spectroscopy lakowicz free download: Chlorophyll a Fluorescence G.C. Papageorgiou, Govindjee, 2007-11-12 Chlorophyll a Fluorescence: A Signature of Photosynthesis highlights chlorophyll (Chl) a fluorescence as a convenient, non-invasive, highly sensitive, rapid and quantitative probe of oxygenic photosynthesis. Thirty-one chapters, authored by 58 international experts, provide a solid foundation of the basic theory, as well as of the application of the rich information contained in the Chl a fluorescence signal as it relates to photosynthesis and plant productivity. Although the primary photochemical reactions of photosynthesis are highly efficient, a small fraction of absorbed photons escapes as Chl fluorescence, and this fraction varies with metabolic state, providing a basis for monitoring quantitatively various processes of photosynthesis. The book explains the mechanisms with which plants defend themselves against environmental stresses (excessive light, extreme temperatures, drought, hyper-osmolarity, heavy metals and UV). It also includes discussion on fluorescence imaging of leaves and cells and the remote sensing of Chl fluorescence from terrestrial, airborne, and satellite bases. The book is intended for use by graduate students, beginning researchers and advanced undergraduates in the areas of integrative plant biology, cellular and molecular biology, plant biology, biochemistry, biophysics, plant physiology, global ecology and agriculture. |
| principles of fluorescence spectroscopy lakowicz free download: Fluorescence Methods for Investigation of Living Cells and Microorganisms Natalia Grigoryeva, 2020-09 Fluorescence methods play a leading role in the investigation of biological objects. They are the only non-destructive methods for investigating living cells and microorganisms in vivo. Using intrinsic and artificial fluorescence methods provides deep insight into mechanisms underlying physiological and biochemical processes. This book covers a wide range of modern methods involved in experimental biology. It illustrates the use of fluorescence microscopy and spectroscopy, confocal laser scanning microscopy, flow cytometry, delayed fluorescence, pulse-amplitude-modulation fluorometry, and fluorescent dye staining protocols. This book provides an overview of practical and theoretical aspects of fluorescence methods and their successful application in the investigation of static and dynamic processes in living cells and microorganisms. |
| principles of fluorescence spectroscopy lakowicz free download: An Introduction to Fluorescence Correlation Spectroscopy Thorsten Wohland, 2020-11-05 An Introduction to Fluorescence Correlation Spectroscopy represents a comprehensive introduction to fluorescence correlation spectroscopy (FCS), a biophysical experimental technique increasingly used to study and quantify molecular mobility, concentrations and interactions in vitro, as well as in living cells and multicellular organisms. Students and researchers who are new to FCS can use the book as the first introduction to the technique, while those who are already using FCS regularly in their research may find it useful to deepen their understanding of the technique, its possibilities, limitations, and potential pitfalls as well as ways to avoid them. This book introduces the reader to all aspects of FCS needed for practical usage of the technique in their research. In the beginning the concept of fluorescence intensity fluctuations and their auto- and cross-correlation functions are explained to give readers an understanding of the underlying principles. This is followed by an overview of instrumental FCS setups and various ways of data collection and processing, the derivations of theoretical models relating the experimentally obtained correlation functions to the underlying molecular processes, and the description of the fitting of experimental data with those models. Mathematically more involved portions are separated from the rest of the text and can be easily skipped by readers more interested in the conceptual and practical aspects of FCS. The book contains interactive graphics and is accompanied by an interactive computable document file allowing the reader to test the dependence of FCS results on a variety of experimental parameters, and to gain practical insights into FCS data fitting. Key Features Introduces the concepts of FCS in an accessible way, supported by animations and graphics in the ebook. Includes a supplementary interactive computable document file that allows the reader to experiment with various FCS setup and fit parameters, allowing readers to test their understanding and simulate experimental outcomes. Provides rigorous mathematical derivations of fundamental FCS equations and models. Pedagogical features include questions, short reviews and critical discussions of literature relevant to the particular chapter that include applications and fundamental developments in the field of FCS. |
| principles of fluorescence spectroscopy lakowicz free download: Topics in Fluorescence Spectroscopy Joseph R. Lakowicz, 1991 |
| principles of fluorescence spectroscopy lakowicz free download: Fluorescence Lifetime Imaging Ophthalmoscopy Martin Zinkernagel, Chantal Dysli, 2019-07-29 This book focuses on the emerging non-invasive imaging technique of Fluorescence Lifetime Imaging Ophthalmoscopy (FLIO). FLIO reveals unique information on retinal diseases, ranging from age-related macular degeneration and vascular diseases to hereditary retinal dystrophies. Fluorescence lifetimes enable the evaluation of disease progression before irreversible structural changes occur. The image acquisition is suitable for diagnostic purposes and follow-up examinations to investigate the natural course of disease, and to monitor the effects of possible therapies. This book fills the gap between available literature and gives state-of-the-art guidance on the principles of the FLIO technique, image acquisition, and data analysis. Written by a team of expert leaders within this field, this book will be relevant for scientists and clinicians with an interest in ophthalmoscopy. |
| principles of fluorescence spectroscopy lakowicz free download: Luminescence Jagannathan Thirumalai, 2016-11-10 The aim of this book is to give readers a broad review of topical worldwide advancements in theoretical and experimental facts, instrumentation and practical applications erudite by luminescent materials and their prospects in dealing with different types of luminescence like photoluminescence, electroluminescence, thermo-luminescence, triboluminescence, bioluminescence design and applications. The additional part of this book deals with the dynamics, rare-earth ions, photon down-/up-converting materials, luminescence dating, lifetime, bioluminescence microscopical perspectives and prospects towards the basic research or for more advanced applications. This book is divided into four main sections: luminescent materials and their associated phenomena; photo-physical properties and their emerging applications; thermoluminescence dating: from theory to applications, and bioluminescence perspectives and prospects. Individual chapters should serve the broad spectrum of common readers of diverse expertise, layman, students and researchers, who may in this book find easily elucidated fundamentals as well as progressive principles of specific subjects associated with these phenomena. This book was created by 14 contributions from experts in different fields of luminescence and technology from over 20 research institutes worldwide. |
| principles of fluorescence spectroscopy lakowicz free download: Fluorescence Sensors and Biosensors Richard B. Thompson, 2019-08-30 Fluorescence-based sensing is a significant technique used in prominent fields such as fluorescence-activated cell sorting, DNA sequencing, high-throughput screening, and clinical diagnostics. Fluorescence Sensors and Biosensors emphasizes the most recent developments and emerging technologies with the broadest impacts. The text begins with the development of aptamers (oligoribonucleotides) and biorecognition techniques based on periplasmic binding proteins. The following chapters review the molecular beacon approach for DNA recognition, describe resonance energy transfer (FRET) in sensing, and present the use of carbonic anhydrase recognition platform for metal ion determination and imaging. The book explores the advantages of fluorophores, fluorescent labels, sensor and assay construction, metal-enhanced fluorescence, phosphorescent labels, and lab-on-a-chip applications. It also describes new anion-selective fluorescent probes used as analytes in clinical determinations. The final chapters highlight the application of fluorescence sensing technology to several practical problems, such as the development of planar waveguide biosensors for clinical diagnostics and the adaptation of fluorescence-based sensing approaches for biochemical production by fermentation. The book also discusses the measurement of analytes, such as free zinc ions, at ultratrace levels in biological specimens. Written by internationally renowned authors in their fields, Fluorescence Sensors and Biosensors provides an up-to-date account of fluorescence-based sensors focused on practical applications in biotechnology, analytical chemistry, and biomedicine. |
| principles of fluorescence spectroscopy lakowicz free download: Biochemical Fluorescence Raymond F. Chen, Harold Edelhoch, 1975 |
| principles of fluorescence spectroscopy lakowicz free download: Introduction to the Statistical Dynamics of Automatic Control Systems Vladimir Viktorovich Solodovnikov, 1960 |
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Ray Dalio, one of the world’s most successful investors and entrepreneurs, shares the unconventional principles that helped him create unique results in life and business.
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A principle is a kind of rule, belief, or idea that guides you. You can also say a good, ethical person has a lot of principles.
PRINCIPLE | English meaning - Cambridge Dictionary
Principal as an adjective means ‘most important’: … [ C ] His guiding principle is that everyone should have equal access to high-quality health care. [ C ] He refused to compromise his …
Principle - Wikipedia
There are many principles observed in physics, notably in cosmology which observes the mediocrity principle, the anthropic principle, the principle of relativity and the cosmological …
PRINCIPLE Definition & Meaning | Dictionary.com
Principle, canon, rule imply something established as a standard or test, for measuring, regulating, or guiding conduct or practice. A principle is a general and fundamental truth that …
PRINCIPLE definition and meaning | Collins English Dictionary
A principle is a general belief that you have about the way you should behave, which influences your behaviour. Buck never allowed himself to be bullied into doing anything that went against …
principle noun - Definition, pictures, pronunciation and usage …
Definition of principle noun from the Oxford Advanced Learner's Dictionary. [countable, usually plural, uncountable] a moral rule or a strong belief that influences your actions. He has high …
Principle Definition & Meaning | Britannica Dictionary
In principle, making the changes should be a simple matter, but there may be problems we haven't thought of. They accepted the offer in principle. Do not confuse principle with principal.
Principle Definition & Meaning - YourDictionary
Principle definition: A basic truth, law, or assumption.
