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| plant cell and tissue culture: Plant Cell and Tissue Culture Indra K. Vasil, Trevor A. Thorpe, 2013-03-09 Plant Cell and Tissue Culture gives an exhaustive account of plant cell culture and genetic transformation, including detailed chapters on all major field and plantation crops. Part A presents a comprehensive coverage of all necessary laboratory techniques for the initiation, nutrition, maintenance and storage of plant cell and tissue cultures, including discussions on these topics, as well as on morphogenesis and regeneration, meristem and shoot tip culture, plant protoplasts, mutant cell lines, variation in tissue cultures, isogenic lines, fertilization control, cryopreservation, transformation, and the production of secondary metabolites. Part B then proceeds into detail on the specific in vitro culture of specific crops, including cereals, legumes, vegetables, potatoes, other roots and tubers, oilseeds, temperate fruits, tropical fruits, plantation crops, forest trees and ornamentals. Plant Cell and Tissue Culture is, and is likely to remain, the laboratory manual of choice, as well as a source of inspiration and a guide to all workers in the field. |
| plant cell and tissue culture: Plant Cell, Tissue and Organ Culture Oluf L. Gamborg, Gregory Phillips, 2014-03-12 This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operation and management as well as a glossary and all the information needed to set up and carry out any of the procedures without having to use other resource books. It is especially designed for professionals and advanced students who wish to acquire practical skills and first-hand experience in plant biotechnology. |
| plant cell and tissue culture: Plant Tissue Culture Robert H. Smith, 2012-12-02 Plant Tissue Culture Techniques and Experiments is a manual that contains laboratory exercises about the demonstration of the methods and different plant materials used in plant tissue culture. It provides an overview on the plant cell culture techniques and plant material options in selecting the explant source. This book starts by discussing the proper setup of a tissue culture laboratory and the selection of the culture medium. It then explains the determination of an explant which is the ultimate goal of the cell culture project. The explant is a piece of plant tissue that is used in tissue culture. Furthermore, the book discusses topics about callus induction, regeneration and morphogenesis process, and haploid plants from anther and pollen culture. The meristem culture for virus-free plants and in vitro propagation for commercial propagation of ornamentals are also explained in this manual. The book also provides topics and exercises on the protoplast isolation and fusion and agrobacterium-mediated transformation of plants. This manual is intended for college students, both graduate and undergraduate, who study chemistry, plant anatomy, and plant physiology. |
| plant cell and tissue culture: Introduction to Plant Tissue Culture M. K. Razdan, 2003 Introduction and techniques; Introductory history; Laboratory organisation; Media; Aseptic manipulation; Basic aspects; Cell culture; Cellular totipotency; Somatic embryogenesis; Applications to plant breeding; Haploid prodution; Triploid production; In vitro pollination and fertilization; Zygotic embryo culture; Somatic hybridisation and cybridisation; Genetic transformation; Somaclonal and gametoclonal variant selection; Application to horticulture and forestry; Production of disease-free plants; clonal propagation; General applications; Industrial applications: secondary metabolite production; Germplasm conservation. |
| plant cell and tissue culture: Plant Cell and Tissue Culture - A Tool in Biotechnology Karl-Hermann Neumann, Ashwani Kumar, Jafargholi Imani, 2009-04-28 This book provides a general introduction as well as a selected survey of key advances in the fascinating field of plant cell and tissue culture as a tool in biotechnology. After a detailed description of the various basic techniques employed in leading laboratories worldwide, follows an extended account of important applications in, for example, plant propagation, secondary metabolite production and gene technology. Additionally, some chapters are devoted to historical developments in this domain, metabolic aspects, nutrition, growth regulators, differentiation and the development of culture systems. The book will prove useful to both newcomers and specialists, and even “old hands” in tissue culture should find some challenging ideas to think about. |
| plant cell and tissue culture: Plant Cell Culture David E. Evans, Julian O. D. Coleman, Anne Kearns, 2003 Plant cell culture is an essential methodology in plant sciences, with numerous variant techniques depending on the cell type and organism. Plant Cell Culture provides the reader with a concise overview of these techniques, including basic plant biology for cell culture, basic sterile technique and media preparation, specific techniques for various plant cell and tissue types including applications, tissue culture in agriculture, horticulture and forestry and culture for genetic engineering and biotechnology. This book will be an essential addition to any plant science laboratory's bookshelf. |
| plant cell and tissue culture: Plant Cell Culture Michael R. Davey, Paul Anthony, 2010-05-20 The ability to culture cells is fundamental for mass propagation and as a baseline for the genetic manipulation of plant nuclei and organelles. The introduction to Plant Cell Culture: Essential Methods provides a general background to plant cell culture, including basic principles, technologies and laboratory practices that underpin the more detailed techniques described in subsequent chapters. Whilst each chapter provides a background to the topic area and methodology, a crucial aspect is the provision of detailed protocols with emphasis on trouble shooting, describing common problems and detailed advice for their avoidance. Plant Cell Culture: Essential Methods provides the reader with a concise overview of these techniques, including micropropagation, mutagenesis, cryopreservation, genetic and plastid transformation and somatic cell technologies. This book will be an essential addition to any plant science laboratory's bookshelf. Highlights the best and most up-to-date techniques for working on plant cell culture Explains clearly and precisely how to carry out selected techniques in addition to background information on the various approaches Chapters are written by leading international authorities in the field and cover both well-known and new, tried and tested, methods for working in plant cell culture An essential laboratory manual for students and early-career researchers. |
| plant cell and tissue culture: Plant Cell Culture Protocols Víctor M. Loyola-Vargas, Felipe Vázquez-Flota, 2008-02-04 A comprehensive state-of-the-art collection of the most frequently used techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods range from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation involving the laborious process of protoplast isolation and culture. Most of the protocols are currently used in the research programs of the authors or represent important parts of business projects aimed at the generation of improved plant materials. Two new appendices explain the principles for formulating culture media and the composition of the eight most commonly used media formulations, and list more than 100 very useful internet sites. |
| plant cell and tissue culture: Plant Cell Culture Protocols Víctor M. Loyola-Vargas, Neftalí Ochoa-Alejo, 2012-05-10 Cell culture methodologies have become standard procedures in most plant laboratories. Currently, facilities for in vitro cell cultures are found in practically every plant biology laboratory, serving different purposes since tissue culture has turned into a basic asset for modern biotechnology, from the fundamental biochemical aspects to the massive propagation of selected individuals. “Plant Cell Culture Protocols, Third Edition is divided into five convenient sections that cover topics from general methodologies, such as culture induction, growth and viability evaluation, statistical analysis and contamination control, to highly specialized techniques, such as clonal propagation, haploid production, somatic embryogenesis, organelle transformation. The volume concludes with a section on the laborious process of measuring the epigenetics changes in tissue cultures.”Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Plant Cell Culture Protocols, Third Edition seeks to serve both professionals and novices with its guide to the most common and applicable techniques and methods for plant tissue and cell culture. |
| plant cell and tissue culture: Plant Tissue Culture and Its Bio-technological Application W. Barz, E. Reinhard, M.H. Zenk, 2012-12-06 |
| plant cell and tissue culture: Modern Applications of Plant Biotechnology in Pharmaceutical Sciences Saurabh Bhatia, Kiran Sharma, Randhir Dahiya, Tanmoy Bera, 2015-07-22 Modern Applications of Plant Biotechnology in Pharmaceutical Sciences explores advanced techniques in plant biotechnology, their applications to pharmaceutical sciences, and how these methods can lead to more effective, safe, and affordable drugs. The book covers modern approaches in a practical, step-by-step manner, and includes illustrations, examples, and case studies to enhance understanding. Key topics include plant-made pharmaceuticals, classical and non-classical techniques for secondary metabolite production in plant cell culture and their relevance to pharmaceutical science, edible vaccines, novel delivery systems for plant-based products, international industry regulatory guidelines, and more. Readers will find the book to be a comprehensive and valuable resource for the study of modern plant biotechnology approaches and their pharmaceutical applications. - Builds upon the basic concepts of cell and plant tissue culture and recombinant DNA technology to better illustrate the modern and potential applications of plant biotechnology to the pharmaceutical sciences - Provides detailed yet practical coverage of complex techniques, such as micropropogation, gene transfer, and biosynthesis - Examines critical issues of international importance and offers real-life examples and potential solutions |
| plant cell and tissue culture: Plant Cell Culture Hamish A. Collin, Sue Edwards, 1998 All the information necessary to set up and run a tissue culture facility is provided in this introductory book.; ; Includes an overview of all the basic tissue culture techniques and describes in detail both the theoretical background and the practical a |
| plant cell and tissue culture: Plant Cell and Tissue Culture J. Reinert, M.M. Yeoman, 2012-12-06 The techniques of plant organ, tissue, and cell culture concentrated on reproducibility, simplicity and accu are now established in many research laboratories racy with sufficient illustration to make all mani throughout the world and are being used in numerous pulations clear. areas of plant science. Methods have been developed The drawings of items used in the bench layout to propagate plants and free them from viruses using diagrams are symbolic and are 'keyed in' by number to shoot tip culture. The regeneration of plants from callus the list of materials and equipment. A line around an culture has also proved useful commercially. Elegant item indicates that is sterile. techniques have been used to synthesise somatic The adoption of an integrated text in which diagrams hybrids by the fusion of protoplasts and to transform are related spatially to the methods will, we hope, help cells. These and many other techniques have been the student to grasp the techniques quickly and effec and can be used to investigate a variety of botanical tively. This is first and foremost a manual which has its phenomena as well as to improve crop plants and now place on the laboratory bench open in front of the provide an important part of the basic experimental student, a book to be used! skills required by a majority of experimental botanists. |
| plant cell and tissue culture: Plant Tissue Culture: An Introductory Text Sant Saran Bhojwani, Prem Kumar Dantu, 2013-04-06 Plant tissue culture (PTC) is basic to all plant biotechnologies and is an exciting area of basic and applied sciences with considerable scope for further research. PTC is also the best approach to demonstrate the totipotency of plant cells, and to exploit it for numerous practical applications. It offers technologies for crop improvement (Haploid and Triploid production, In Vitro Fertilization, Hybrid Embryo Rescue, Variant Selection), clonal propagation (Micropropagation), virus elimination (Shoot Tip Culture), germplasm conservation, production of industrial phytochemicals, and regeneration of plants from genetically manipulated cells by recombinant DNA technology (Genetic Engineering) or cell fusion (Somatic Hybridization and Cybridization). Considerable work is being done to understand the physiology and genetics of in vitro embryogenesis and organogenesis using model systems, especially Arabidopsis and carrot, which is likely to enhance the efficiency of in vitro regeneration protocols. All these aspects are covered extensively in the present book. Since the first book on Plant Tissue Culture by Prof. P.R. White in 1943, several volumes describing different aspects of PTC have been published. Most of these are compilation of invited articles by different experts or proceedings of conferences. More recently, a number of books describing the Methods and Protocols for one or more techniques of PTC have been published which should serve as useful laboratory manuals. The impetus for writing this book was to make available a complete and up-to-date text covering all basic and applied aspects of PTC for the students and early-career researchers of plant sciences and plant / agricultural biotechnology. The book comprises of nineteen chapters profusely illustrated with self-explanatory illustrations. Most of the chapters include well-tested protocols and relevant media compositions that should be helpful in conducting laboratory experiments. For those interested in further details, Suggested Further Reading is given at the end of each chapter, and a Subject and Plant Index is provided at the end of the book. |
| plant cell and tissue culture: Plant Cell Culture R. A. Dixon, Robert A. Gonzales, 1994 Plant cell culture techniques are used increasingly in basic research for plant exploitation in industry, including for example, genetic engineering and micropropagation. The rapidly developing role of plant cell culture has necessitated this new edition of a widely acclaimed book. It covers a wide range of methods central to the exploitation of plant cell cultures in fundamental and applied research. This thoroughly revised work retains the combination of giving and explaining the general principles involved with the concise description of specific protocols, with appeal to a broad readership, that made the first edition so successful. Internationally recognized experts describe the techniques used for isolating and manipulating cell cultures, and the central importance in plant biotechnology. The book will be of major interest to researchers in plant sciences in general, and specifically to botany, plant physiology, and biotechnology students. |
| plant cell and tissue culture: Applications of Plant Cell and Tissue Culture Gregory R. Bock, Joan Marsh, 2008-04-30 This work deals with basic plant physiology and cytology, and addresses the practical exploitation of plants, both as crops and as sources of useful compounds produced as secondary metabolites. Covers problems of commercial exploitation, socio-legal aspects of genetic engineering of crop plants, and of the difficulties of marketing natural compunds produced by cells under artificial conditions. |
| plant cell and tissue culture: Plant Tissue Culture Timir Baran Jha, 2005 Plant Tissue Culture In One Form Or Another Has Become One Of The Most Promising Branches Of Plant Science. Arising From The Totipotency Of Plant Cells, It Now Occupies A Key Position In Plant Breeding, Plant Propagation And Plant Biotechnology.Plant Tissue Culture - Basic And Applied Brings To The Student Accessible, Up-To-Date Information On This Subject. Basic Knowledge Of Tissue Culture Methods Such As Isolation Of Suitable Tissues From The Mother Plant, Maintenance Of The Tissues Under In Vitro Condition In An Undifferentiated Or De-Differentiated Stage, Methods Of Genetic Engineering And Gene Transfer, Chromosomal Studies And The Handling Of In Vitro Micro Plants Are Described In Detail In This Book. Similarly, Application Aspects Of Micropropagation, Haploid Cell Culture, Protoplast Culture, Embryo Culture, Somatic Embryogenesis And Artificial Seeds Are Also Discussed. |
| plant cell and tissue culture: Plant Cell Culture Secondary MetabolismToward Industrial Application Frank DiCosmo, Masanaru Misawa, 1996-07-10 Plant cell cultures are used extensively in studies of secondary metabolism, for the biosynthesis of pharmaceuticals, flavors, essences, and pigments. This book highlights recent developments in the in vitro growth of cultured plant cells and in the production of valuable secondary metabolites. Plant Cell Culture Secondary Metabolism details research on many exciting areas including: |
| plant cell and tissue culture: Plant Tissue Culture Margit Laimer, Waltraud Rücker, 2012-12-06 In 2002 the 100th anniversary of the publication on Culturversuche mit isolierten Pflanzenzellen by Gottlieb Haberlandt was celebrated. Haberlandt ́s vision of the totipotency of plant cells represents the actual beginning of tissue culture. This book pays homage to a great Austrian scientist and the further development of his ideas. The first part of the book contains a facsimile of the original paper which is a true artistic masterpiece and its first translation into English from 1969. The second and third parts describe Haberlandt ́s life and work and early historical aspects of the development of plant tissue culture. The fourth part of the book contains an overview of important topics of plant tissue culture with the most promising areas of application to date and an outlook into the future. Areas range from micropropagation, production of pharmaceutically interesting compounds, plant breeding, genetic engineering of crop plants, including trees, and cryopreservation of valuable germplasm. |
| plant cell and tissue culture: Experiments in Plant Tissue Culture John H. Dodds, Lorin W. Roberts, 1985-10-31 The second edition of Experiments in Plant Tissue Culture makes available new information that has resulted from recent advances in the applications of plant tissue culture techniques to agriculture and industry. This comprehensive laboratory text takes the reader through a graded series of experimental protocols and also provides an introductory review of each topic. Topics include: a plant tissue culture laboratory, aseptic techniques, nutritional components of media, callus induction, organ formation, xylem cell differentiation, root cultures, cell suspensions, micropropagation, embryogenesis, isolation and fusion of protoplasts, haploid cultures, storage of plant genetic resources, secondary metabolite production, and quantification of procedures. This volume offers all of the basic experimental methods for the major research areas of plant tissue culture, and it will be invaluable to undergraduates and research investigators in the plant sciences. |
| plant cell and tissue culture: Phytochemicals in Plant Cell Cultures Indra K. Vasil, 2012-12-02 Cell Culture and Somatic Cell Genetics of Plants, Volume 5: Phytochemicals in Plant Cell Cultures provides comprehensive coverage of the wide variety of laboratory procedures used in plant cell culture, fundamental aspects of cell growth and nutrition, and plant regeneration and variability. This book consists of five main topics—phenylpropanoids, naphthoquinones, and anthraquinones; mevalonates; alkaloids; glucosinolates, polyacetylenes, and lipids; and biologically active compounds. This publication specifically discusses the coumarins in crown gall tumors, natural occurrence of bufadienolides, and accumulation of protoberberine alkaloids. The flavor production in tissue cultures of allium species and callus cultures derived from carrot root explants is also reviewed. This volume is valuable to experienced researchers and those newly entering the field of plant cell and tissue culture. |
| plant cell and tissue culture: Plant Tissue Culture Sant Saran Bhojwani, Vibha Dhawan, Renu Arora, 1990 The first volume of this Plant Tissue Culture (PTC) bibliography, published in 1986, covered the literature from 1900 to 1985. This supplement to the PTC bibliography covers the papers and books published in the period 1986-1989, with some references of 1985 and before which were not available when the original volume was compiled. The detailed Introduction to this volume includes a table with a graphic presentation of the trend of research in the field of PTC during this 5-year period. It is interesting to note that during the past five years alone about 6000 papers have been published, which is almost half of the literature published from 1900 to 1985. Another table lists the periodicals and their respective volumes surveyed for this book. Appended is an Index to Plant Names, which facilitates the search of the literature either subject-wise or species/crop-wise. |
| plant cell and tissue culture: Plant Tissue Culture Roberta H. Smith, 2013 Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples. The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists. Provides new exercises demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation for other experimental systems Includes detailed drawings that complement both introductions and experiments Guides reader from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement Provides the latest techniques and media information, including A. tumefaciens mediated transformation and infusion technology Fully updated literature |
| plant cell and tissue culture: Dictionary of Plant Tissue Culture Alan Cassells, Peter B. Gahan, 2006-09-25 Concise definitions and descriptions to more than 1,000 terms—illustrated and fully referenced The Dictionary of Plant Tissue Culture is a user-friendly A-to-Z resource for the technical terms that apply to plant cell, tissue, and organ culture. Filled with illustrations of key concepts and references to up-to-date textbooks, papers, and reviews, this handy guidebook defines both the terms that come from plant tissue culture, and those that originate from plant anatomy, genetics, stress physiology, growth regular research, microbiology, and plant pathology. It’s an essential resource for anyone involved in tissue culture or using plant tissue culture systems for plant cloning, secondary metabolic production, plant pathology, and genetic manipulations. The Dictionary of Plant Tissue Culture is an invaluable reference tool for teachers, students, and researchers working in basic and applied plant tissue culture. The book’s entries are cross-referenced where appropriate, with references mainly to general textbooks on plant anatomy, biochemistry and histochemistry, developmental biology, genetics, microbiology, micropropagation, plant breeding, plant biotechnology, plant pathology and plant tissue culture. From ABA (abscisic acid) to zygotic embryo, this concise glossary will save you valuable time in your efforts to find concise definitions and descriptions to more than 1,000 terms. Entries to the Dictionary of Plant Tissue Culture include: acclimatization caulogenesis feeder cells hyperhydricity indexing micro-grafting phytosanitary certificate re-invigoration synseed totipotency and much more! The Dictionary of Plant Tissue Culture is an essential reference resource for undergraduate and postgraduate students, teachers, researchers, and technicians working in plant tissue culture and genetic engineering. |
| plant cell and tissue culture: Plant Propagation by Tissue Culture: In practice Edwin F. George, 1993 |
| plant cell and tissue culture: In Vitro Cultivation of Plant Cells Open Universiteit (Heerlen, Netherlands), 1993 This text aims to provide the essential knowledge of the core processes involved in the cultivation of plant cells and tissues in vitro thereby enabling readers to understand and contribute to the practical application of these techniques. The text begins by introducing the key terms used in plant tissue culture and provides an overview of the range and objectives of plant tissue culture studies. Subsequent chapters provide knowledge of the processes of the major techniques developed within the framework of the perspectives described in the introductory chapter. The important issues of media formulation and preparation are described together with discussion of the influence of plant growth regulators on the growth and development of plant cell systems in vitro. |
| plant cell and tissue culture: Plant Tissue Culture Engineering S. Dutta Gupta, Yasuomi Ibaraki, 2006-07-10 It is my privilege to contribute the foreword for this unique volume entitled: “Plant Tissue Culture Engineering,” edited by S. Dutta Gupta and Y. Ibaraki. While there have been a number of volumes published regarding the basic methods and applications of plant tissue and cell culture technologies, and even considerable attention provided to bioreactor design, relatively little attention has been afforded to the engineering principles that have emerged as critical contributions to the commercial applications of plant biotechnologies. This volume, “Plant Tissue Culture Engineering,” signals a turning point: the recognition that this specialized field of plant science must be integrated with engineering principles in order to develop efficient, cost effective, and large scale applications of these technologies. I am most impressed with the organization of this volume, and the extensive list of chapters contributed by expert authors from around the world who are leading the emergence of this interdisciplinary enterprise. The editors are to be commended for their skilful crafting of this important volume. The first two parts provide the basic information that is relevant to the field as a whole, the following two parts elaborate on these principles, and the last part elaborates on specific technologies or applications. |
| plant cell and tissue culture: Plant Tissue Culture, Development, and Biotechnology Robert N. Trigiano, Dennis J. Gray, 2011-06-30 Under the vast umbrella of Plant Sciences resides a plethora of highly specialized fields. Botanists, agronomists, horticulturists, geneticists, and physiologists each employ a different approach to the study of plants and each for a different end goal. Yet all will find themselves in the laboratory engaging in what can broadly be termed biotechnology. Addressing a wide variety of related topics, Plant Tissue Culture, Development, and Biotechnology gives the practical and technical knowledge needed to train the next generation of plant scientists regardless of their ultimate specialization. With the detailed perspectives and hands-on training signature to the authors’ previous bestselling books, Plant Development and Biotechnology and Plant Tissue Culture Concepts and Laboratory Exercises, this book discusses relevant concepts supported by demonstrative laboratory experiments. It provides critical thinking questions, concept boxes highlighting important ideas, and procedure boxes giving precise instruction for experiments, including step-by-step procedures, such as the proper microscope use with digital photography, along with anticipated results, and a list of materials needed to perform them. Integrating traditional plant sciences with recent advances in plant tissue culture, development, and biotechnology, chapters address germplasm preservation, plant growth regulators, embryo rescue, micropropagation of roses, haploid cultures, and transformation of meristems. Going beyond the scope of a simple laboratory manual, this book also considers special topics such as copyrights, patents, legalities, trade secrets, and the business of biotechnology. Focusing on plant culture development and its applications in biotechnology across a myriad of plant science specialties, this text uses a broad range of species and practical laboratory exercises to make it useful for anyone engaged in the plant sciences. |
| plant cell and tissue culture: Recent Advances in Plant in vitro Culture Annarita Leva, Laura Rinaldi, 2012-10-17 The purpose of this book is to provide the advances in plant in vitro culture as related to perennial fruit crops and medicinal plants. Basic principles and new techniques, now available, are presented in detail. The book will be of use to researchers, teachers in biotechnology and for individuals interested to the commercial application of plant in vitro culture. |
| plant cell and tissue culture: Plant Tissue Culture Manual K. Lindsey, 1991 Basic techniques - cells tissue culture of model species. Tissue culture & transformation of crop species. Propagation & conservation of germplasm. Direct gene transfer & protoplast fusion. Reproductive tissues. Mutant selection. |
| plant cell and tissue culture: Plant Cell Culture Protocols Robert D. Hall, 2008-02-02 Robert Hall and a panel of expert researchers present a comprehensive collection of the most frequently used and broadly applicable techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods cover culture initiation, maintenance, manipulation, application, and long-term storage, with emphasis on techniques for genetic modification and micropropagation. Many of these protocols are currently used in major projects designed to produce improved varieties of important crop plants. Plant Cell Culture Protocols's state-of-the-art techniques are certain to make the book today's reference of choice, an indispensable tool in the development of new transgenic plants and full-scale commercial applications. |
| plant cell and tissue culture: Cells and Tissues in Culture E. N. Willmer, 2015-12-04 Cells and Tissues in Culture: Methods, Biology and Physiology, Volume 1 covers the general fields of tissue culture, including an evaluation of its technique, effects, and contributions to biology. This book focuses on the three methods of culture—tissue culture, cell culture, and organ culture.Other topics include the design of complete synthetic media, possible evolution of the cell types, and energy relationships in growing and stationary cells. The RNA synthesis in cell cultures, culture of amphibian embryonic anlage, action of corticosteroids and adrenaline, and effects of parathyroid hormone on bone are also elaborated. This volume is recommended for biologists and specialists interested in the culture of cells and tissues. |
| plant cell and tissue culture: Plant Tissue Culture Masanaru Misawa, 1994-01-01 |
| plant cell and tissue culture: Plant Pathology Mark T. Windham, Robert N. Trigiano, Alan S. Windham, 2003-07-28 Instructors, students and researchers in plant pathology have been searching for a primary text that combines an informal, easy-to-read style with a thorough introduction to the concepts and terminology of plant pathology. Plant Pathology Concepts and Laboratory Exercises answers their demand by presenting pathology principles, protocols and procedures, serving as a valuable resource tool for both students and researchers. This guide explains definitions of disease, characteristics of organisms that cause disease, and how diseases interact with hosts and the environment. Each topic is addressed by an expert in the field, and is supported by one or more lab exercises. The structure of the text allows for easy reading, with references minimized and major concepts highlighted at the beginning of each chapter. The laboratory exercises give added flexibility to instructors. There are experiments for both beginning and advanced students, and a broad choice of exercise topics that can be selected based upon the focus within each individual class. Step-by-step instructions are provided for each laboratory exercise. |
| plant cell and tissue culture: Industrial Biotechnology Mukesh Yadav, Vikas Kumar, Nirmala Sehrawat, 2019-10-08 Industrial Biotechnology summarizes different aspects of plant biotechnology such as using plants as sustainable resources, phytomedical applications, phytoremedation and genetic engineering of plant systems. These topics are discussed from an academic as well industrial perspective and thus highlight recent developments but also practical aspects of modern biotechnology. |
| plant cell and tissue culture: Plant Tissue and Cell Culture Herbert Edward Street, 1973 |
| plant cell and tissue culture: Plant Tissue Culture Roberta H. Smith, 2012-10-22 Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples. The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists. - Provides new exercises demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation for other experimental systems - Includes detailed drawings that complement both introductions and experiments - Guides reader from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement - Provides the latest techniques and media information, including A. tumefaciens mediated transformation and infusion technology - Fully updated literature |
Plant - Wikipedia
Plants are the eukaryotes that form the kingdom Plantae; they are predominantly photosynthetic. This means that they obtain their energy from sunlight, using chloroplasts derived from …
Plant | Definition, Evolution, Ecology, & Taxonomy | Britannica
May 26, 2025 · Plant, any multicellular, eukaryotic, usually photosynthetic life-form in the kingdom Plantae. There are an estimated 390,900 different species of plants known to science. Learn …
Plant - Definition, Characteristics and Types | Biology Dictionary
Apr 28, 2017 · Plant Definition. Plants are multicellular organisms in the kingdom Plantae that use photosynthesis to make their own food. There are over 300,000 species of plants; common …
Plant - Simple English Wikipedia, the free encyclopedia
Plants include familiar types such as trees, herbs, bushes, grasses, vines, ferns, mosses, and green algae. The scientific study of plants, known as botany, has identified about 391,000 …
NYFA: New York Flora Atlas
May 23, 2025 · The New York Flora Atlas is a source of information for the distribution of plants within the state, as well as information on plant habitats, associated ecological communities, …
Plants - NYSDEC - New York State Department of Environmental …
New York State's many habitats, both on land and water, shelter rich and diverse plant life. From the beloved sugar maple to the common milkweed, both humans and wildlife depend on the …
Plants: The Ultimate Guide To The Plant Kingdom, With Pictures …
Apr 9, 2020 · On this page you’ll find a complete guide to plants and the plant kingdom. You’ll discover what makes a plant a plant, why plants are important, the different types of plant and …
What is a plant? — Science Learning Hub
The word ‘plant’ encompasses a wide range of living organisms, all of which belong to the kingdom Plantae and share a range of characteristics. In this article, we examine the key …
Plant Definition and Examples - Biology Online Dictionary
May 14, 2024 · Plants are eukaryotic organisms that are photosynthetic and with a rigid cell wall. Learn the definition of a plant, its structure, taxonomic groups, characteristics, evolution, and …
Types of Plants: Different Kinds of Plants and Their Classification
Jun 12, 2020 · Discover different types of plants and plant groups, such as flowering plants, ornamental grasses, ferns and more.
Plant - Wikipedia
Plants are the eukaryotes that form the kingdom Plantae; they are predominantly photosynthetic. This means that they obtain their energy from sunlight, using chloroplasts derived from …
Plant | Definition, Evolution, Ecology, & Taxonomy | Britannica
May 26, 2025 · Plant, any multicellular, eukaryotic, usually photosynthetic life-form in the kingdom Plantae. There are an estimated 390,900 different species of plants known to science. Learn …
Plant - Definition, Characteristics and Types | Biology Dictionary
Apr 28, 2017 · Plant Definition. Plants are multicellular organisms in the kingdom Plantae that use photosynthesis to make their own food. There are over 300,000 species of plants; common …
Plant - Simple English Wikipedia, the free encyclopedia
Plants include familiar types such as trees, herbs, bushes, grasses, vines, ferns, mosses, and green algae. The scientific study of plants, known as botany, has identified about 391,000 …
NYFA: New York Flora Atlas
May 23, 2025 · The New York Flora Atlas is a source of information for the distribution of plants within the state, as well as information on plant habitats, associated ecological communities, …
Plants - NYSDEC - New York State Department of Environmental …
New York State's many habitats, both on land and water, shelter rich and diverse plant life. From the beloved sugar maple to the common milkweed, both humans and wildlife depend on the …
Plants: The Ultimate Guide To The Plant Kingdom, With …
Apr 9, 2020 · On this page you’ll find a complete guide to plants and the plant kingdom. You’ll discover what makes a plant a plant, why plants are important, the different types of plant and …
What is a plant? — Science Learning Hub
The word ‘plant’ encompasses a wide range of living organisms, all of which belong to the kingdom Plantae and share a range of characteristics. In this article, we examine the key …
Plant Definition and Examples - Biology Online Dictionary
May 14, 2024 · Plants are eukaryotic organisms that are photosynthetic and with a rigid cell wall. Learn the definition of a plant, its structure, taxonomic groups, characteristics, evolution, and …
Types of Plants: Different Kinds of Plants and Their Classification
Jun 12, 2020 · Discover different types of plants and plant groups, such as flowering plants, ornamental grasses, ferns and more.
