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| plant tissue culture: techniques and experiments: Plant Tissue Culture Robert H. Smith, 2012-12-02 Plant Tissue Culture Techniques and Experiments is a manual that contains laboratory exercises about the demonstration of the methods and different plant materials used in plant tissue culture. It provides an overview on the plant cell culture techniques and plant material options in selecting the explant source. This book starts by discussing the proper setup of a tissue culture laboratory and the selection of the culture medium. It then explains the determination of an explant which is the ultimate goal of the cell culture project. The explant is a piece of plant tissue that is used in tissue culture. Furthermore, the book discusses topics about callus induction, regeneration and morphogenesis process, and haploid plants from anther and pollen culture. The meristem culture for virus-free plants and in vitro propagation for commercial propagation of ornamentals are also explained in this manual. The book also provides topics and exercises on the protoplast isolation and fusion and agrobacterium-mediated transformation of plants. This manual is intended for college students, both graduate and undergraduate, who study chemistry, plant anatomy, and plant physiology. |
| plant tissue culture: techniques and experiments: Plant Tissue Culture Roberta H. Smith, 2000 This manual provides laboratory exercises in plant tissue culture which demonstrate major educational concepts. It includes sections on scheduling and interrelationships of exercises, tissue culture setup, supplies and media. |
| plant tissue culture: techniques and experiments: Plant Tissue Culture-Techniques & Experiments, 2E Roberta H. Smith, 2005-01-01 |
| plant tissue culture: techniques and experiments: Plant Tissue Culture Roberta H. Smith, 2012-10-22 Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples. The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists. - Provides new exercises demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation for other experimental systems - Includes detailed drawings that complement both introductions and experiments - Guides reader from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement - Provides the latest techniques and media information, including A. tumefaciens mediated transformation and infusion technology - Fully updated literature |
| plant tissue culture: techniques and experiments: Experiments in Plant Tissue Culture John H. Dodds, Lorin W. Roberts, 1985-10-31 The second edition of Experiments in Plant Tissue Culture makes available new information that has resulted from recent advances in the applications of plant tissue culture techniques to agriculture and industry. This comprehensive laboratory text takes the reader through a graded series of experimental protocols and also provides an introductory review of each topic. Topics include: a plant tissue culture laboratory, aseptic techniques, nutritional components of media, callus induction, organ formation, xylem cell differentiation, root cultures, cell suspensions, micropropagation, embryogenesis, isolation and fusion of protoplasts, haploid cultures, storage of plant genetic resources, secondary metabolite production, and quantification of procedures. This volume offers all of the basic experimental methods for the major research areas of plant tissue culture, and it will be invaluable to undergraduates and research investigators in the plant sciences. |
| plant tissue culture: techniques and experiments: Plant Tissue Culture Sunghun Park, 2021-02-19 Plant Tissue Culture: Techniques and Experiments, Fourth Edition, builds on the classroom tested, audience proven manual that has guided users through successful plant culturing for almost 30 years. The book's experiments demonstrate major concepts and can be conducted with a variety of plant materials readily available throughout the year. This fully updated edition describes the principles of the newest technologies, including CRISPR/Cas9 gene editing and RNAi technology with plant cell and tissue cultures and their applications. Bridging the gap between theory and practice, this book contains detailed methodology supported by comprehensive illustrations, giving users a diverse learning experience for both university students and plant scientists. |
| plant tissue culture: techniques and experiments: Introduction to Plant Tissue Culture M. K. Razdan, 2003 Introduction and techniques; Introductory history; Laboratory organisation; Media; Aseptic manipulation; Basic aspects; Cell culture; Cellular totipotency; Somatic embryogenesis; Applications to plant breeding; Haploid prodution; Triploid production; In vitro pollination and fertilization; Zygotic embryo culture; Somatic hybridisation and cybridisation; Genetic transformation; Somaclonal and gametoclonal variant selection; Application to horticulture and forestry; Production of disease-free plants; clonal propagation; General applications; Industrial applications: secondary metabolite production; Germplasm conservation. |
| plant tissue culture: techniques and experiments: Plant Tissue Culture Roberta H. Smith, 2012-08-03 Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples. The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists. Provides new exercises demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation for other experimental systems Includes detailed drawings that complement both introductions and experiments Guides reader from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement Provides the latest techniques and media information, including A. tumefaciens mediated transformation and infusion technology Fully updated literature. |
| plant tissue culture: techniques and experiments: Plants from Test Tubes Lydiane Kyte, 1983 |
| plant tissue culture: techniques and experiments: Plant Pathology Mark T. Windham, Robert N. Trigiano, Alan S. Windham, 2003-07-28 Instructors, students and researchers in plant pathology have been searching for a primary text that combines an informal, easy-to-read style with a thorough introduction to the concepts and terminology of plant pathology. Plant Pathology Concepts and Laboratory Exercises answers their demand by presenting pathology principles, protocols and procedures, serving as a valuable resource tool for both students and researchers. This guide explains definitions of disease, characteristics of organisms that cause disease, and how diseases interact with hosts and the environment. Each topic is addressed by an expert in the field, and is supported by one or more lab exercises. The structure of the text allows for easy reading, with references minimized and major concepts highlighted at the beginning of each chapter. The laboratory exercises give added flexibility to instructors. There are experiments for both beginning and advanced students, and a broad choice of exercise topics that can be selected based upon the focus within each individual class. Step-by-step instructions are provided for each laboratory exercise. |
| plant tissue culture: techniques and experiments: Plant Tissue Culture: An Introductory Text Sant Saran Bhojwani, Prem Kumar Dantu, 2013-04-06 Plant tissue culture (PTC) is basic to all plant biotechnologies and is an exciting area of basic and applied sciences with considerable scope for further research. PTC is also the best approach to demonstrate the totipotency of plant cells, and to exploit it for numerous practical applications. It offers technologies for crop improvement (Haploid and Triploid production, In Vitro Fertilization, Hybrid Embryo Rescue, Variant Selection), clonal propagation (Micropropagation), virus elimination (Shoot Tip Culture), germplasm conservation, production of industrial phytochemicals, and regeneration of plants from genetically manipulated cells by recombinant DNA technology (Genetic Engineering) or cell fusion (Somatic Hybridization and Cybridization). Considerable work is being done to understand the physiology and genetics of in vitro embryogenesis and organogenesis using model systems, especially Arabidopsis and carrot, which is likely to enhance the efficiency of in vitro regeneration protocols. All these aspects are covered extensively in the present book. Since the first book on Plant Tissue Culture by Prof. P.R. White in 1943, several volumes describing different aspects of PTC have been published. Most of these are compilation of invited articles by different experts or proceedings of conferences. More recently, a number of books describing the Methods and Protocols for one or more techniques of PTC have been published which should serve as useful laboratory manuals. The impetus for writing this book was to make available a complete and up-to-date text covering all basic and applied aspects of PTC for the students and early-career researchers of plant sciences and plant / agricultural biotechnology. The book comprises of nineteen chapters profusely illustrated with self-explanatory illustrations. Most of the chapters include well-tested protocols and relevant media compositions that should be helpful in conducting laboratory experiments. For those interested in further details, Suggested Further Reading is given at the end of each chapter, and a Subject and Plant Index is provided at the end of the book. |
| plant tissue culture: techniques and experiments: Plant Tissue Culture Edward E Johnson, 2020-12-23 Do you want to know how to tissue culture plants and grow more in less space? If so this how-to guide is for you. Plant tissue culture can be done at home without expensive lab grade gear. Inside, you will find easy and affordable alternatives to supplies and equipment that would otherwise be unobtainable to most. The return in numbers of plants for your investment is very lucrative and rewarding, not to mention easy.Anyone that can cook dinner can practice micropropagation of plants in a compact space and in incredible numbers. Anyone that has seen the exploding price of houseplants and recreational plants can see what a reward growing thousands of plants yourself can bring.What you need to start a successful lab at home in a compact spaceHow to use your equipment and supplies as easily as possibleWhat each stage does and how to easily perform the tasksHow to get your favorite plants into tissue cultureWhy you should be using plant tissue culture to grow to your potentialHow to grow out your tissue cultured plants for outside or saleAquarium plants, houseplants, garden plants, recreational plants, carnivorous plants, orchids, mosses, and more can quickly and easily be multiplied. Many plants you see at garden centers are propagated by plant tissue culture and you can do it too!Turn one plant into thousands quickly. In the amount of time it takes to grow a cutting to produce new shoots to make more cuttings you can have hundreds of plants in many species. Plant tissue culture allows the multiplication of your prized plants exponentially. It also allows you to use a kitchen corner or a small room as a lab area that will give you positive results.Keep up with the demand and changing tastes of the plant hobby. Propagate plants faster with tissue culture and keep up with your demand for more plants. |
| plant tissue culture: techniques and experiments: Automation and environmental control in plant tissue culture Jenny Aitken-Christie, T. Kozai, M.A.L Smith, 1995 Automation in plant tissue culture; General introduction and overview; Economic analysis of automated micropropagation; Economic aspects of somati embryogenesis; Systems analysis and engineering; Engineering aspects of plant propagation in bioreactors; Mechanical engineering approaches to plant biotechnology; Image analysis for plant cell culture and micropropagation; Image analysis for embryogenesis; Automation of the bioreactor process for mass propagation and secondary metabolism; Delivery system for tissue culture by encapsulation; A delivery system for naked somatic embryos for interior spruce; Automated systems for organogenesis; Commercialisation of tissue culture and automated systems; Environmental control in plant tissue culture; General introduction; Physical microenvironmental adn its effects; Vessels, gels, liquid media, and support systems; The chemical mciroenvironment; Carbon nutrition in vitro; Regulation and manupulation of carbon assimilation in micropropagated systems; Ethylene; In vitro acclimatization; Low temperature storage of plant tissue cultures; Environmental measurement and control systems. |
| plant tissue culture: techniques and experiments: Modern Applications of Plant Biotechnology in Pharmaceutical Sciences Saurabh Bhatia, Kiran Sharma, Randhir Dahiya, Tanmoy Bera, 2015-07-22 Modern Applications of Plant Biotechnology in Pharmaceutical Sciences explores advanced techniques in plant biotechnology, their applications to pharmaceutical sciences, and how these methods can lead to more effective, safe, and affordable drugs. The book covers modern approaches in a practical, step-by-step manner, and includes illustrations, examples, and case studies to enhance understanding. Key topics include plant-made pharmaceuticals, classical and non-classical techniques for secondary metabolite production in plant cell culture and their relevance to pharmaceutical science, edible vaccines, novel delivery systems for plant-based products, international industry regulatory guidelines, and more. Readers will find the book to be a comprehensive and valuable resource for the study of modern plant biotechnology approaches and their pharmaceutical applications. - Builds upon the basic concepts of cell and plant tissue culture and recombinant DNA technology to better illustrate the modern and potential applications of plant biotechnology to the pharmaceutical sciences - Provides detailed yet practical coverage of complex techniques, such as micropropogation, gene transfer, and biosynthesis - Examines critical issues of international importance and offers real-life examples and potential solutions |
| plant tissue culture: techniques and experiments: Plant Tissue Culture Margit Laimer, Waltraud Rücker, 2012-12-06 In 2002 the 100th anniversary of the publication on Culturversuche mit isolierten Pflanzenzellen by Gottlieb Haberlandt was celebrated. Haberlandt ́s vision of the totipotency of plant cells represents the actual beginning of tissue culture. This book pays homage to a great Austrian scientist and the further development of his ideas. The first part of the book contains a facsimile of the original paper which is a true artistic masterpiece and its first translation into English from 1969. The second and third parts describe Haberlandt ́s life and work and early historical aspects of the development of plant tissue culture. The fourth part of the book contains an overview of important topics of plant tissue culture with the most promising areas of application to date and an outlook into the future. Areas range from micropropagation, production of pharmaceutically interesting compounds, plant breeding, genetic engineering of crop plants, including trees, and cryopreservation of valuable germplasm. |
| plant tissue culture: techniques and experiments: Plant Propagation by Tissue Culture: In practice Edwin F. George, 1993 |
| plant tissue culture: techniques and experiments: Practical manual for Plant Tissue Culture Hirenkumar Sherathiya, 2013-11-27 Document from the year 2012 in the subject Agrarian Studies, , course: Carrier Oriented Program, language: English, abstract: Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition. Different techniques in plant tissue culture may offer certain advantages over traditional methods of propagation. This practical manual has been prepared in response to the necessities of the graduate students as an introduction to the in vitro tissue culture techniques and some molecular aspects. |
| plant tissue culture: techniques and experiments: Industrial Biotechnology Mukesh Yadav, Vikas Kumar, Nirmala Sehrawat, 2019-10-08 Industrial Biotechnology summarizes different aspects of plant biotechnology such as using plants as sustainable resources, phytomedical applications, phytoremedation and genetic engineering of plant systems. These topics are discussed from an academic as well industrial perspective and thus highlight recent developments but also practical aspects of modern biotechnology. |
| plant tissue culture: techniques and experiments: Practical Biotechnology and Plant Tissue Culture Madhavi Adhav, 2009-07 The book starts with an introduction to basic knowledge of instruments which deals with principle, working, uses, limitations and precautions of about ten instruments.Basic Knowledge of precaution of;Culture Media for Bacterial Growth, Plant Tissue Culture and Standard Solutions has been given in simple and easy-to-follow language.The biotechnology exercises such as Plasmid and DNA isolation, DNA size determination, Restriction digestion, PCR, Gus gene assay, RFLP, RAPD, Isolation of bacteria by streak and Pour plate method, Growth characteristics of E.Coli by Plating and Turbidimetric method and the plant tissues culture exercises such as Cell suspension culture, Androgenesis, Somatic embryogenesis, Preparation of plantlet to greenhouse field, have been given in a student friendly manner. Matter for Viva-voce has also been included. |
| plant tissue culture: techniques and experiments: Recent Advances in Plant in vitro Culture Annarita Leva, Laura Rinaldi, 2012-10-17 The purpose of this book is to provide the advances in plant in vitro culture as related to perennial fruit crops and medicinal plants. Basic principles and new techniques, now available, are presented in detail. The book will be of use to researchers, teachers in biotechnology and for individuals interested to the commercial application of plant in vitro culture. |
| plant tissue culture: techniques and experiments: Biotechnology Procedures and Experiments Handbook S. Harisha, 2008-12 Biotechnology Is One Of The Major New Technologies Of The Twenty-First Century That Covers Multi-Disciplinary Issues, Including Recombinant DNA Techniques, Cloning, Genetics, And The Application Of Microbiology To The Production Of Goods. It Continues To Revolutionize Treatments Of Many Diseases, And It Is Used To Deal With Environmental Solutions. The Biotechnology Procedures And Experiments Handbook Provides Practicing Professionals And Biotechnology Students Over 150 Applied, Up-To-Date Laboratory Techniques And Experiments Related To Modern Topics Such As Recombinant DNA, Electrophoresis, Stem Cell Research, Genetic Engineering, Microbiology, Tissue Culture, And More. Each Lab Technique Includes 1)A Principle, 2)The Necessary Reagents, 3)A Step By Step Procedure, And 4)A Final Result. Also Included Is A Section That Shows How To Avoid Potential Pitfalls Of A Specific Experiment. The Book Is Accompanied By A CD-ROM Containing Simulations, White Papers, And Other Relevant Material To Biotechnology. |
| plant tissue culture: techniques and experiments: Inorganic Plant Nutrition A. Läuchli, R.L. Bieleski, 2012-12-06 The first book bearing the title of this volume, Inorganic Plant Nutrition, was written by D. R. HOAGLAND of the University of California at Berkeley. As indicated by its extended title, Lectures on the Inorganic Nutrition of Plants, it is a collection of lectures - the JOHN M. PRATHER lectures, which he was invited in 1942 to give. at Harvard University and presented there between April 10 and 23 of that year - 41 years before the publication of the present volume. They were not originally intended for publication but fortunately HOAGLAND was persuaded to publish them; the book appeared in 1944. It might at first blush seem inappropriate to draw comparisons between a book embodying a set of lectures by a single author and an encyclopedic volume with no less than 37 contributors. But HOAGLAND'S book was a compre hensive account of the state of this science in his time, as the present volume is for ours. It was then still possible for one person, at least for a person of HOAGLAND'S intellectual breadth and catholicity of interests, to encompass many major areas of the entire field, from the soil substrate to the metabolic roles of nitrogen, potassium, and other nutrients, and from basic scientific topics to the application of plant nutritional research in solving problems encountered in the field. |
| plant tissue culture: techniques and experiments: Tissue Culture Techniques for Horticultural Crops Kenneth C. Torres, 2012-12-06 This book was written for those individuals who are concerned about the techniques and practices of plant cell cultures for horticultural crops. It was designed to serve as a text and reference for students and professionals in ornamental horticulture, fruit and vegetable crop pro duction, botany, forestry, and other areas of plant science. Research during the last twenty-five years in the area of plant tissue culture has led to many developments and changes in this field. Al though the techniques involved in the manipulation of plant tissue culture are now relatively straightforward, the presentation of these techniques in a short volume for the beginner in the field is generally unavailable. In addition to describing the techniques for establishment and manipulation of specific species, several chapters in this book also provide a brief, general review of important cultural parameters. Spe cific protocols and laboratory procedures may also be found in the appendix. I hope that this presentation of information will be helpful to those individuals wanting to apply plant tissue culture techniques for horticultural crops. |
| plant tissue culture: techniques and experiments: Physiology, Growth and Development of Plants in Culture P.J. Lumsden, J.R. Nicholas, W.J. Davies, 2012-12-06 Over recent years, progress in micropropagation has not been as rapid as many expected and, even now, relatively few crops are produced commercially. One reason for this is that the biology of material growing in vitro has been insufficiently understood for modifications to standard methods to be made based on sound physiological principles. However, during the past decade, tissue culture companies and others have invested considerable effort to reduce the empirical nature of the production process. The idea of the conference `Physiology, Growth and Development of Plants and Cells in Culture' (Lancaster, 1992) was to introduce specialists in different areas of plant physiology to micropropagators, with the express aims of disseminating as wide a range of information to as large a number of participants as possible, and beginning new discussions on the constraints and potentials affecting the development of in vitro plant production methods. This book is based on presentations from the conference and has been divided into two main sections, dealing with either aspects of the in vitro environment -- light, nutrients, water, gas -- or with applied aspects of the culture process -- morphogenesis, acclimation, rejuvenation, contamination. |
| plant tissue culture: techniques and experiments: Liquid Culture Systems for in vitro Plant Propagation A.K. Hvoslef-Eide, W. Preil, 2005-06-15 High-efficiency micropropagation, with relatively low labour costs, has been demonstrated in this unique book detailing liquid media systems for plant tissue culture. World authorities (e.g. von Arnold, Curtis, Takayama, Ziv) contribute seminal papers together with papers from researchers across Europe that are members of the EU COST Action 843 Advanced micropropagation systems. First-hand practical applications are detailed for crops – including ornamentals and trees – using a wide range of techniques, from thin-film temporary immersion systems to more traditional aerated bioreactors with many types of explant – shoots to somatic embryos. The accounts are realistic, balanced and provide a contemporary account of this important aspect of mass propagation. This book is essential reading for all those in commercial micropropagation labs, as well as researchers worldwide who are keen to improve propagation techniques and lower economic costs of production. Undergraduate and postgraduate students in the applied plant sciences and horticulture will find the book an enlightened treatise. |
| plant tissue culture: techniques and experiments: Plant Cell and Tissue Culture - A Tool in Biotechnology Karl-Hermann Neumann, Ashwani Kumar, Jafargholi Imani, 2009-04-28 This book provides a general introduction as well as a selected survey of key advances in the fascinating field of plant cell and tissue culture as a tool in biotechnology. After a detailed description of the various basic techniques employed in leading laboratories worldwide, follows an extended account of important applications in, for example, plant propagation, secondary metabolite production and gene technology. Additionally, some chapters are devoted to historical developments in this domain, metabolic aspects, nutrition, growth regulators, differentiation and the development of culture systems. The book will prove useful to both newcomers and specialists, and even “old hands” in tissue culture should find some challenging ideas to think about. |
| plant tissue culture: techniques and experiments: Plant Tissue Culture Techniques and Experiments Josie Ho (ed), 2016 |
| plant tissue culture: techniques and experiments: Plant Tissue Culture and Its Bio-technological Application W. Barz, E. Reinhard, M.H. Zenk, 2012-12-06 |
| plant tissue culture: techniques and experiments: Pathogen and Microbial Contamination Management in Micropropagation Alan C. Cassells, 1997-10-31 This book is based mainly on invited and offered papers presented at the Second International Symposium on Bacterial and Bacteria-like Contaminants of Plant Tissue Cultures held at University College, Cork, Ireland in September 1996, with additional invited papers. The First International Symposium on Bacterial and Bacteria-like Contaminants of Plant Tissue Cultures was held at the same venue in 1987 and was published as Acta Horticulturae volume 225, 1988. In the intervening years there have been considerable advances in both plant disease diagnostics and in the development of structured approaches to the management of disease and microbial contamination in micropropagation. These approaches have centred on attempts to separate, spatially, the problems of disease transmission and laboratory contamination. Disease-control is best achieved by establishing pathogen-free cultures while laboratory contamination is based on subsequent good working practice. Control of losses due to pathogens and microbial contamination in vitro addresses, arguably, the most importance causes of losses in the industry; nevertheless, losses at and post establishment can also be considerable due to poor quality microplants or micro-shoots. In this symposium, a holistic approach to pathogen and microbial contamination control is evident with the recognition that micropropagators must address pathogen and microbial contamination in vitro, and diseases and microplant failure at establishment. There is increasing interest in establishing beneficial bacterial and mycorrhizal association with microplants in vitro and in vivo. |
| plant tissue culture: techniques and experiments: Tissue Culture as a Plant Production System for Horticultural Crops Richard H. Zimmerman, 1986-07-31 Conference on Tissue Culture as a Plant Production System for Horticultural Crops, Beltsville, MD, October 20-23, 1985 |
| plant tissue culture: techniques and experiments: Plant Propagation Concepts and Laboratory Exercises Caula A. Beyl, Robert N. Trigiano, 2016-01-06 Includes a DVD Containing All Figures and Supplemental Images in PowerPointThis new edition of Plant Propagation Concepts and Laboratory Exercises presents a robust view of modern plant propagation practices such as vegetable grafting and micropropagation. Along with foundation knowledge in anatomy and plant physiology, the book takes a look into t |
| plant tissue culture: techniques and experiments: Plant Tissue Culture Manual K. Lindsey, 1991 Basic techniques - cells tissue culture of model species. Tissue culture & transformation of crop species. Propagation & conservation of germplasm. Direct gene transfer & protoplast fusion. Reproductive tissues. Mutant selection. |
| plant tissue culture: techniques and experiments: Plant Cell and Tissue Culture Indra K. Vasil, Trevor A. Thorpe, 2013-03-09 Plant Cell and Tissue Culture gives an exhaustive account of plant cell culture and genetic transformation, including detailed chapters on all major field and plantation crops. Part A presents a comprehensive coverage of all necessary laboratory techniques for the initiation, nutrition, maintenance and storage of plant cell and tissue cultures, including discussions on these topics, as well as on morphogenesis and regeneration, meristem and shoot tip culture, plant protoplasts, mutant cell lines, variation in tissue cultures, isogenic lines, fertilization control, cryopreservation, transformation, and the production of secondary metabolites. Part B then proceeds into detail on the specific in vitro culture of specific crops, including cereals, legumes, vegetables, potatoes, other roots and tubers, oilseeds, temperate fruits, tropical fruits, plantation crops, forest trees and ornamentals. Plant Cell and Tissue Culture is, and is likely to remain, the laboratory manual of choice, as well as a source of inspiration and a guide to all workers in the field. |
| plant tissue culture: techniques and experiments: Plant Tissue Culture: Propagation, Conservation and Crop Improvement Mohammad Anis, Naseem Ahmad, 2018-07-05 This book presents basic concepts, methodologies and applications of biotechnology for the conservation and propagation of aromatic, medicinal and other economic plants. It caters to the needs and challenges of researchers in plant biology, biotechnology, the medical sciences, pharmaceutical biotechnology and pharmacology areas by providing an accessible and cost-effective practical approach to micro-propagation and conservation strategies for plant species. It also includes illustrations describing a complete documentation of the results and research into particular plant species conducted by the authors over the past 5 years. Plant Biotechnology has been a subject of academic interest for a considerable time. In recent years, it has also become a useful tool in agriculture and medicine, as well as a popular area of biological research. Current economic growth is globally projected in a highly positive manner, but the challenges many countries face with regard to food, feed, malnutrition, infectious diseases, the newly identified life-style diseases, and energy shortages, all of which are worsened by an ever-deteriorating environment, continue to pull the growth digits back. The common thread that connects all of the above challenges is biotechnology, which could provide many answers. Molecular biology and biotechnology have now become an integral part of tissue culture research. The tremendous impact generated by genetic engineering and consequently of transgenics now allows us to manipulate plant genomes at will. There has indeed been a rapid development in this area with major successes in both developed and developing countries. The book introduces several new and exciting areas to researchers who are unfamiliar with plant biotechnology and also serves as a review of ongoing research and future directions for scholars. The book highlights numerous methods for in vitro propagation and utilization of techniques in raising transgenics to help readers reproduce the experiments discussed. |
| plant tissue culture: techniques and experiments: Cotton Physiology Jack R. Mauney, James McD. Stewart, 1986 |
| plant tissue culture: techniques and experiments: Experiments in Microbiology, Plant Pathology, Tissue Culture and Mushroom Production Technology K. R. Aneja, 2001 |
| plant tissue culture: techniques and experiments: Mulberry Maharaj Krishen Razdan, Dennis T. Thomas, 2021-07-26 Mulberry (Morus spp.) is an important horticultural plant in the sericulture industry. It belongs to the family Moraceae. The leaf of mulberry is used to feed the silkworm Bombyx mori L. It is also used as a fodder. Due to its economic and agricultural importance, mulberry is cultivated in many parts of the world. An estimated 60% of the total cost of silk cocoon production is for production and maintenance of mulberry plants. Therefore, much attention is needed to improve the quality and quantity of mulberry leaves. It is vital to increase the production of superior quality mulberry leaves with high nutritive value for the sericulture industry. Although a lot of research is going on in mulberry, very little effort has been made to compile the results of this research in a single book. This book provides an update of recent research works going on in this plant. It describes the taxonomy, conservation of germplasm, genetic diversity of various mulberry species, application of breeding techniques to improve the quality of mulberry, in vitro conservation, application of tissue culture techniques to improve mulberry species, production of haploids and triploids in mulberry and improvement of abiotic stress adaptive traits in mulberry with relevance to adaptiveness to global warming. |
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