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| plant tissue culture lab layout: Introduction to Plant Tissue Culture M. K. Razdan, 2003 Introduction and techniques; Introductory history; Laboratory organisation; Media; Aseptic manipulation; Basic aspects; Cell culture; Cellular totipotency; Somatic embryogenesis; Applications to plant breeding; Haploid prodution; Triploid production; In vitro pollination and fertilization; Zygotic embryo culture; Somatic hybridisation and cybridisation; Genetic transformation; Somaclonal and gametoclonal variant selection; Application to horticulture and forestry; Production of disease-free plants; clonal propagation; General applications; Industrial applications: secondary metabolite production; Germplasm conservation. |
| plant tissue culture lab layout: Plant Cell Culture David E. Evans, Julian O. D. Coleman, Anne Kearns, 2003 Plant cell culture is an essential methodology in plant sciences, with numerous variant techniques depending on the cell type and organism. Plant Cell Culture provides the reader with a concise overview of these techniques, including basic plant biology for cell culture, basic sterile technique and media preparation, specific techniques for various plant cell and tissue types including applications, tissue culture in agriculture, horticulture and forestry and culture for genetic engineering and biotechnology. This book will be an essential addition to any plant science laboratory's bookshelf. |
| plant tissue culture lab layout: Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition Robert N. Trigiano, Dennis J. Gray, 1999-11-10 Alternating between topic discussions and hands-on laboratory experiments that range from the in vitro flowering of roses to tissue culture of ferns, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, addresses the most current principles and methods in plant tissue culture research. The editors use the expertise of some of the top researchers and educators in plant biotechnology to furnish students, instructors and researchers with a broad consideration of the field. Divided into eight major parts, the text covers everything from the history of plant tissue culture and basic methods to propagation techniques, crop improvement procedures, specialized applications and nutrition of callus cultures. New topic discussions and laboratory exercises in the Second Edition include Micropropagation of Dieffenbachia, Micropropagation and in vitro flowering of rose, Propagation from nonmeristematic tissue-organogenesis, Variation in culture and Tissue culture of ferns. It is the book's extensive laboratory exercises that provide a hands-on approach in illustrating various topics of discussion, featuring step-by-step procedures, anticipated results, and a list of materials needed. What's more, editors Trigiano and Gray go beyond mere basic principles of plant tissue culture by including chapters on genetic transformation techniques, and photographic methods and statistical analysis of data. In all, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, is a veritable harvest of information for the continued study and research in plant tissue culture science. |
| plant tissue culture lab layout: Plant Tissue Culture B. N. Sathyanarayana, Dalia B. Varghese, 2007 Plant Tissue Culture forms an integral basis of the present day biotechnology. Plant Tissue Culture: Practices and New Experimental Protocols is being brought out to fill the existing gap in the available literature on plant tissue culture, especially focusing on the aspects of practical procedures and protocols of tissue culture. This book contains important experimental techniques and gives guidance on carrying out hands-on experiences. It has been designed in a simple way, giving all the necessary procedures as a general guideline and also necessary tips to maneuver any problem encountered. These tips are based on the first hand experiences of the author while teaching and researching the techniques of plant tissue culture. A unique feature of this book is the inclusion of several techniques describing the actual protocols experimented and developed with different plant species by different scientists. A substantial number of original colored plates including fluorescence photographs standout the book. This pioneering work is valuable for the students who are looking for fresh outlook and search. |
| plant tissue culture lab layout: Tissue Culture in Forestry J. M. Bonga, D. J. Durzan, 1982 |
| plant tissue culture lab layout: Plant Tissue Culture Robert H. Smith, 2012-12-02 Plant Tissue Culture Techniques and Experiments is a manual that contains laboratory exercises about the demonstration of the methods and different plant materials used in plant tissue culture. It provides an overview on the plant cell culture techniques and plant material options in selecting the explant source. This book starts by discussing the proper setup of a tissue culture laboratory and the selection of the culture medium. It then explains the determination of an explant which is the ultimate goal of the cell culture project. The explant is a piece of plant tissue that is used in tissue culture. Furthermore, the book discusses topics about callus induction, regeneration and morphogenesis process, and haploid plants from anther and pollen culture. The meristem culture for virus-free plants and in vitro propagation for commercial propagation of ornamentals are also explained in this manual. The book also provides topics and exercises on the protoplast isolation and fusion and agrobacterium-mediated transformation of plants. This manual is intended for college students, both graduate and undergraduate, who study chemistry, plant anatomy, and plant physiology. |
| plant tissue culture lab layout: Modern Applications of Plant Biotechnology in Pharmaceutical Sciences Saurabh Bhatia, Kiran Sharma, Randhir Dahiya, Tanmoy Bera, 2015-07-22 Modern Applications of Plant Biotechnology in Pharmaceutical Sciences explores advanced techniques in plant biotechnology, their applications to pharmaceutical sciences, and how these methods can lead to more effective, safe, and affordable drugs. The book covers modern approaches in a practical, step-by-step manner, and includes illustrations, examples, and case studies to enhance understanding. Key topics include plant-made pharmaceuticals, classical and non-classical techniques for secondary metabolite production in plant cell culture and their relevance to pharmaceutical science, edible vaccines, novel delivery systems for plant-based products, international industry regulatory guidelines, and more. Readers will find the book to be a comprehensive and valuable resource for the study of modern plant biotechnology approaches and their pharmaceutical applications. - Builds upon the basic concepts of cell and plant tissue culture and recombinant DNA technology to better illustrate the modern and potential applications of plant biotechnology to the pharmaceutical sciences - Provides detailed yet practical coverage of complex techniques, such as micropropogation, gene transfer, and biosynthesis - Examines critical issues of international importance and offers real-life examples and potential solutions |
| plant tissue culture lab layout: Plant Tissue Culture Timir Baran Jha, 2005 Plant Tissue Culture In One Form Or Another Has Become One Of The Most Promising Branches Of Plant Science. Arising From The Totipotency Of Plant Cells, It Now Occupies A Key Position In Plant Breeding, Plant Propagation And Plant Biotechnology.Plant Tissue Culture - Basic And Applied Brings To The Student Accessible, Up-To-Date Information On This Subject. Basic Knowledge Of Tissue Culture Methods Such As Isolation Of Suitable Tissues From The Mother Plant, Maintenance Of The Tissues Under In Vitro Condition In An Undifferentiated Or De-Differentiated Stage, Methods Of Genetic Engineering And Gene Transfer, Chromosomal Studies And The Handling Of In Vitro Micro Plants Are Described In Detail In This Book. Similarly, Application Aspects Of Micropropagation, Haploid Cell Culture, Protoplast Culture, Embryo Culture, Somatic Embryogenesis And Artificial Seeds Are Also Discussed. |
| plant tissue culture lab layout: Plant Tissue and Cell Culture Herbert Edward Street, 1977 |
| plant tissue culture lab layout: Plant Cell, Tissue and Organ Culture Oluf L. Gamborg, Gregory Phillips, 2014-03-12 This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operation and management as well as a glossary and all the information needed to set up and carry out any of the procedures without having to use other resource books. It is especially designed for professionals and advanced students who wish to acquire practical skills and first-hand experience in plant biotechnology. |
| plant tissue culture lab layout: Low Cost Options for Tissue Culture Technology in Developing Countries , 2004 Describes options for reducing costs in the establishment and operation of plant tissue culture facilities and focuses primarily on plant micropropagation. The publication will be of particular value to the micropropagation industry in developing countries for the enhancement of agricultural productivity to enable sustainable food production. |
| plant tissue culture lab layout: Automation and environmental control in plant tissue culture Jenny Aitken-Christie, T. Kozai, M.A.L Smith, 1995 Automation in plant tissue culture; General introduction and overview; Economic analysis of automated micropropagation; Economic aspects of somati embryogenesis; Systems analysis and engineering; Engineering aspects of plant propagation in bioreactors; Mechanical engineering approaches to plant biotechnology; Image analysis for plant cell culture and micropropagation; Image analysis for embryogenesis; Automation of the bioreactor process for mass propagation and secondary metabolism; Delivery system for tissue culture by encapsulation; A delivery system for naked somatic embryos for interior spruce; Automated systems for organogenesis; Commercialisation of tissue culture and automated systems; Environmental control in plant tissue culture; General introduction; Physical microenvironmental adn its effects; Vessels, gels, liquid media, and support systems; The chemical mciroenvironment; Carbon nutrition in vitro; Regulation and manupulation of carbon assimilation in micropropagated systems; Ethylene; In vitro acclimatization; Low temperature storage of plant tissue cultures; Environmental measurement and control systems. |
| plant tissue culture lab layout: Plant Tissue Culture Roberta H. Smith, 2000 This manual provides laboratory exercises in plant tissue culture which demonstrate major educational concepts. It includes sections on scheduling and interrelationships of exercises, tissue culture setup, supplies and media. |
| plant tissue culture lab layout: Plant Tissue Culture: Theory and Practice S.S. Bhojwani, M.K. Razdan, 1996-11-08 Since the publication of the first edition in 1983, several new and exciting developments have taken place in the field of plant tissue culture, which forms a major component of what is now called plant biotechnology. The revised edition presents updated information on theoretical, practical and applied aspects of plant tissue culture. Each chapter has been thoroughly revised and, as before, is written in lucid language, includes relevant media protocols, and is profusely illustrated with self-explanatory diagrams and original photographs. This book includes three new chapters: Variant selection, Genetic Engineering and Production of Industrial Compounds and contains a complete bibliography and a glossary of terms commonly used in tissue culture literature.This updated version proves to be an excellent text for undergraduate, postgraduate students and teachers in various fields of plant sciences and a useful reference book for those interested in the application of any aspect of this aseptic technology. |
| plant tissue culture lab layout: Biotechnology Procedures and Experiments Handbook S. Harisha, 2008-12 Biotechnology Is One Of The Major New Technologies Of The Twenty-First Century That Covers Multi-Disciplinary Issues, Including Recombinant DNA Techniques, Cloning, Genetics, And The Application Of Microbiology To The Production Of Goods. It Continues To Revolutionize Treatments Of Many Diseases, And It Is Used To Deal With Environmental Solutions. The Biotechnology Procedures And Experiments Handbook Provides Practicing Professionals And Biotechnology Students Over 150 Applied, Up-To-Date Laboratory Techniques And Experiments Related To Modern Topics Such As Recombinant DNA, Electrophoresis, Stem Cell Research, Genetic Engineering, Microbiology, Tissue Culture, And More. Each Lab Technique Includes 1)A Principle, 2)The Necessary Reagents, 3)A Step By Step Procedure, And 4)A Final Result. Also Included Is A Section That Shows How To Avoid Potential Pitfalls Of A Specific Experiment. The Book Is Accompanied By A CD-ROM Containing Simulations, White Papers, And Other Relevant Material To Biotechnology. |
| plant tissue culture lab layout: Plant Propagation by Tissue Culture: In practice Edwin F. George, 1993 |
| plant tissue culture lab layout: Date Palm Biotechnology Shri Mohan Jain, Jameel M. Al-Khayri, Dennis V. Johnson, 2011-08-13 This important reference book is the first comprehensive resource worldwide that reflects research achievements in date palm biotechnology, documenting research events during the last four decades, current status, and future outlook. This book is essential for researchers, policy makers, and commercial entrepreneurs concerned with date palm. The book is invaluable for date palm biotechnology students and specialists. This monument is written by an international team of experienced researchers from both academia and industry. It consists of five sections covering all aspects of date palm biotechnology including A) Micropropagation, B) Somaclonal Variation, Mutation and Selection, C) Germplasm Biodiversity and Conservation, D) Genetics and Genetic Improvement, and E) Metabolites and Industrial Biotechnology. The book brings together the principles and practices of contemporary date palm biotechnology. Each chapter contains background knowledge related to the topic, followed by a comprehensive literature review of research methodology and results including the authors own experience including illustrative tables and photographs. |
| plant tissue culture lab layout: Plant Tissue Culture Edward E Johnson, 2020-12-23 Do you want to know how to tissue culture plants and grow more in less space? If so this how-to guide is for you. Plant tissue culture can be done at home without expensive lab grade gear. Inside, you will find easy and affordable alternatives to supplies and equipment that would otherwise be unobtainable to most. The return in numbers of plants for your investment is very lucrative and rewarding, not to mention easy.Anyone that can cook dinner can practice micropropagation of plants in a compact space and in incredible numbers. Anyone that has seen the exploding price of houseplants and recreational plants can see what a reward growing thousands of plants yourself can bring.What you need to start a successful lab at home in a compact spaceHow to use your equipment and supplies as easily as possibleWhat each stage does and how to easily perform the tasksHow to get your favorite plants into tissue cultureWhy you should be using plant tissue culture to grow to your potentialHow to grow out your tissue cultured plants for outside or saleAquarium plants, houseplants, garden plants, recreational plants, carnivorous plants, orchids, mosses, and more can quickly and easily be multiplied. Many plants you see at garden centers are propagated by plant tissue culture and you can do it too!Turn one plant into thousands quickly. In the amount of time it takes to grow a cutting to produce new shoots to make more cuttings you can have hundreds of plants in many species. Plant tissue culture allows the multiplication of your prized plants exponentially. It also allows you to use a kitchen corner or a small room as a lab area that will give you positive results.Keep up with the demand and changing tastes of the plant hobby. Propagate plants faster with tissue culture and keep up with your demand for more plants. |
| plant tissue culture lab layout: Plant Cell and Tissue Culture J. Reinert, M.M. Yeoman, 2012-12-06 The techniques of plant organ, tissue, and cell culture concentrated on reproducibility, simplicity and accu are now established in many research laboratories racy with sufficient illustration to make all mani throughout the world and are being used in numerous pulations clear. areas of plant science. Methods have been developed The drawings of items used in the bench layout to propagate plants and free them from viruses using diagrams are symbolic and are 'keyed in' by number to shoot tip culture. The regeneration of plants from callus the list of materials and equipment. A line around an culture has also proved useful commercially. Elegant item indicates that is sterile. techniques have been used to synthesise somatic The adoption of an integrated text in which diagrams hybrids by the fusion of protoplasts and to transform are related spatially to the methods will, we hope, help cells. These and many other techniques have been the student to grasp the techniques quickly and effec and can be used to investigate a variety of botanical tively. This is first and foremost a manual which has its phenomena as well as to improve crop plants and now place on the laboratory bench open in front of the provide an important part of the basic experimental student, a book to be used! skills required by a majority of experimental botanists. |
| plant tissue culture lab layout: INTRODUCTION TO PLANT CELL TISSUE AND ORGAN CULTURE SUNIL D. PUROHIT, 2012-10-30 Designed primarily as a text for undergraduate and postgraduate students of Botany and Plant Biotechnology, the book discusses the theoretical aspects and modern applications of plant cell, tissue and organ culture. Written with the aim of providing up-to-date information on the subject, and focused on the concept of commercialization of plant cell culture, the contents have been presented with clarity. The book not only discusses the theoretical aspects of plant tissue culture but also emphasizes the art of its practice. It also provides a systematic explanation of asepsis and methods of sterilization, plant tissue culture techniques, culture of reproductive structures, plant tissue culture in germplasm conservation, its applications in the industry and plant pathology and operation and management of greenhouse hardening unit. In addition, it discusses in vitro propagation of plants (micropropagation) with a series of case studies pertaining to tree species and horticultural crops. Besides students, the book will also prove to be useful for researchers, scholars and teachers. |
| plant tissue culture lab layout: Biotechnology of Plant Tissues P. R. Yadav, Rajiv Tyagi, 2006 Contents: Introduction, Fundamental Clues, Culture Media, Apparatus for Changing Tissue Culture Media, Sterile Technique, Callus Cultures, Protoplast Culture, Haploid Tissue Culture, Organ and Embryo Culture, In Vitro Tissue Culture, In Situ Subculture, Micropropagation, Cell Suspension Culture, Bacterial Culture, Somaclonal Variation, Tissue Culture in Agriculture, Tissue Culture in Genetic Engineeringm Culture of industrially Important Plant Cells, Germplasm Conservation, Prospects and Future Challenges. |
| plant tissue culture lab layout: Cells and Tissues in Culture E. N. Willmer, 2015-12-04 Cells and Tissues in Culture: Methods, Biology and Physiology, Volume 1 covers the general fields of tissue culture, including an evaluation of its technique, effects, and contributions to biology. This book focuses on the three methods of culture—tissue culture, cell culture, and organ culture.Other topics include the design of complete synthetic media, possible evolution of the cell types, and energy relationships in growing and stationary cells. The RNA synthesis in cell cultures, culture of amphibian embryonic anlage, action of corticosteroids and adrenaline, and effects of parathyroid hormone on bone are also elaborated. This volume is recommended for biologists and specialists interested in the culture of cells and tissues. |
| plant tissue culture lab layout: The Cannabis Genome Angel Fernandez i Marti, Jaime Prohens, 2025-06-17 Cannabis is undergoing a remarkable transformation—from a misunderstood plant to a cornerstone of modern medicine, industry, and agriculture. This book bridges the gap between tradition and innovation, providing a holistic exploration of cannabis genetics, breeding, genomics, and its wide-ranging applications. Authored by leading researchers, industry pioneers, and legacy breeders, it offers a unique blend of academic rigor and practical insights. Unlike other resources, this book integrates perspectives from public and private sectors, honoring those who have advanced cannabis cultivation despite legal and social barriers. It recognizes the pivotal role of startups like MyFloraDNA, which invest heavily in groundbreaking research, shaping the future of this extraordinary plant. Key Features: Comprehensive insights into cannabis origins, taxonomy, and genetic diversity. Advanced breeding techniques and applications of tissue culture, CRISPR, and omics technologies. Practical strategies for pest management, crop improvement, and intellectual property protection. Forward-looking perspectives on cannabis in personalized medicine, sustainability, and global agriculture. Written for scientists, growers, policymakers, and enthusiasts, this book addresses the economic, social, and scientific dimensions of cannabis. It offers actionable knowledge to drive progress in cultivation, industry, and research. |
| plant tissue culture lab layout: Plant Tissue Culture A. S. Islam, 1996 The volume contains the Proceedings of the first International Plant Tissue Culture Conference held in Dhaka from December 17-19, 1993. It has two parts: part one comprises articles of a FAO-sponsored session. Assessment and commercialization of in vitro culture techniques for increased and sustained agricultural production; part two comprises papers of the remaining six sessions including a RAPA sponsored one on neem - its multifarious uses and its in vitro micropropagation. |
| plant tissue culture lab layout: Plant Tissue Culture Jayarama Reddy, 2024-02-25 This book is a comprehensive text on plant tissue culture, with its past, present, and future prospects and techniques discussed in detail. In the first three chapters, the history, terminologies, and applications are given in detail. The fourth chapter is dedicated to the instrumentation of plant tissue culture. The basic techniques used in PTC are described in the sixth chapter. The details of the constituents and types of different nutrient media are discussed in the eighth chapter. In chapter number 9, methods of haploid production have been described. Bioreactors are the instruments that are used for the large-scale production of plantlets and plant products. This book is useful for all the students, researchers, teachers, and industrialists interested in plant tissue culture. |
| plant tissue culture lab layout: In Vitro Production of Plant Secondary Metabolites M. Anuradha, S. Balasubramanya, 2025-03-21 This book addresses the literature gap by providing both theoretical foundations and practical insights into in vitro plant secondary metabolite production. It emphasizes the urgency to address the strategies and scientific methods for producing the secondary metabolites in the laboratory conditions. This book includes chapters on various in vitro strategies and laboratory methods, which are crucial for understanding and implementing techniques in this applied field. It equips the readers with the knowledge essential for contributions to pharmaceuticals, agriculture and various industries, thus filling a critical void currently present in the literature and practical applications in this field. This book is structured to cater to a diverse audience, including graduate students and researchers. It is specifically tailored to address the key aspects of plant cell, tissue and organ culture, and aligns seamlessly with the curriculum requirements for the courses offered in plant biotechnology. The modules on cell cultures, secondary metabolite production, and metabolic engineering are also integral components for these courses. |
| plant tissue culture lab layout: Plants from Test Tubes Lydiane Kyte, 1983 |
| plant tissue culture lab layout: Plant Tissue Culture: An Introductory Text Sant Saran Bhojwani, Prem Kumar Dantu, 2013-03-20 Plant tissue culture (PTC) is basic to all plant biotechnologies and is an exciting area of basic and applied sciences with considerable scope for further research. PTC is also the best approach to demonstrate the totipotency of plant cells, and to exploit it for numerous practical applications. It offers technologies for crop improvement (Haploid and Triploid production, In Vitro Fertilization, Hybrid Embryo Rescue, Variant Selection), clonal propagation (Micropropagation), virus elimination (Shoot Tip Culture), germplasm conservation, production of industrial phytochemicals, and regeneration of plants from genetically manipulated cells by recombinant DNA technology (Genetic Engineering) or cell fusion (Somatic Hybridization and Cybridization). Considerable work is being done to understand the physiology and genetics of in vitro embryogenesis and organogenesis using model systems, especially Arabidopsis and carrot, which is likely to enhance the efficiency of in vitro regeneration protocols. All these aspects are covered extensively in the present book. Since the first book on Plant Tissue Culture by Prof. P.R. White in 1943, several volumes describing different aspects of PTC have been published. Most of these are compilation of invited articles by different experts or proceedings of conferences. More recently, a number of books describing the Methods and Protocols for one or more techniques of PTC have been published which should serve as useful laboratory manuals. The impetus for writing this book was to make available a complete and up-to-date text covering all basic and applied aspects of PTC for the students and early-career researchers of plant sciences and plant / agricultural biotechnology. The book comprises of nineteen chapters profusely illustrated with self-explanatory illustrations. Most of the chapters include well-tested protocols and relevant media compositions that should be helpful in conducting laboratory experiments. For those interested in further details, Suggested Further Reading is given at the end of each chapter, and a Subject and Plant Index is provided at the end of the book. |
| plant tissue culture lab layout: Introduction to Plant Biotechnology H. S. Chawla, 2002 Plant biotechnology has created unprecedented opportunities for the manipulation of biological systems of plants. To understand biotechnology, it is essential to know the basic aspects of genes and their organization in the genome of plant cells. This text on the subject is aimed at students. |
| plant tissue culture lab layout: Recent Advances in Plant in vitro Culture Annarita Leva, Laura Rinaldi, 2012-10-17 The purpose of this book is to provide the advances in plant in vitro culture as related to perennial fruit crops and medicinal plants. Basic principles and new techniques, now available, are presented in detail. The book will be of use to researchers, teachers in biotechnology and for individuals interested to the commercial application of plant in vitro culture. |
| plant tissue culture lab layout: Introduction to Cell and Tissue Culture Jennie P. Mather, Penelope E. Roberts, 2007-08-20 It is a pleasure to contribute the foreword to Introduction to Cell and Tissue Culture: The ory and Techniques by Mather and Roberts. Despite the occasional appearance of thought ful works devoted to elementary or advanced cell culture methodology, a place remains for a comprehensive and definitive volume that can be used to advantage by both the novice and the expert in the field. In this book, Mather and Roberts present the relevant method ology within a conceptual framework of cell biology, genetics, nutrition, endocrinology, and physiology that renders technical cell culture information in a comprehensive, logical for mat. This allows topics to be presented with an emphasis on troubleshooting problems from a basis of understanding the underlying theory. The material is presented in a way that is adaptable to student use in formal courses; it also should be functional when used on a daily basis by professional cell culturists in a- demia and industry. The volume includes references to relevant Internet sites and other use ful sources of information. In addition to the fundamentals, attention is also given to mod ern applications and approaches to cell culture derivation, medium formulation, culture scale-up, and biotechnology, presented by scientists who are pioneers in these areas. With this volume, it should be possible to establish and maintain a cell culture laboratory devot ed to any of the many disciplines to which cell culture methodology is applicable. |
| plant tissue culture lab layout: Bridger Plant Materials Center Annual Technical Report , 1993 |
| plant tissue culture lab layout: Pathogen and Microbial Contamination Management in Micropropagation Alan C. Cassells, 1997-10-31 This book is based mainly on invited and offered papers presented at the Second International Symposium on Bacterial and Bacteria-like Contaminants of Plant Tissue Cultures held at University College, Cork, Ireland in September 1996, with additional invited papers. The First International Symposium on Bacterial and Bacteria-like Contaminants of Plant Tissue Cultures was held at the same venue in 1987 and was published as Acta Horticulturae volume 225, 1988. In the intervening years there have been considerable advances in both plant disease diagnostics and in the development of structured approaches to the management of disease and microbial contamination in micropropagation. These approaches have centred on attempts to separate, spatially, the problems of disease transmission and laboratory contamination. Disease-control is best achieved by establishing pathogen-free cultures while laboratory contamination is based on subsequent good working practice. Control of losses due to pathogens and microbial contamination in vitro addresses, arguably, the most importance causes of losses in the industry; nevertheless, losses at and post establishment can also be considerable due to poor quality microplants or micro-shoots. In this symposium, a holistic approach to pathogen and microbial contamination control is evident with the recognition that micropropagators must address pathogen and microbial contamination in vitro, and diseases and microplant failure at establishment. There is increasing interest in establishing beneficial bacterial and mycorrhizal association with microplants in vitro and in vivo. |
| plant tissue culture lab layout: Cell and Tissue Culture Alan Doyle, J. Bryan Griffiths, 1998-11-18 Cell and Tissue Culture: Laboratory Procedures in BiotechnologyEdited by Alan Doyle Centre for Applied Microbiology &Research, Porton Down, Salisbury, UK. and J. Bryan GriffithsScientific Consultancy & Publishing, Porton, Salisbury, UK.Cell and Tissue Culture: Laboratory Procedures in Biotechnologyintroduces the reader to animal cell culture methods describing thekey cells, core techniques, how to scale up the culture forcommercial production, and regulatory aspects. This book provideseasy to follow, step-by-step protocols, with trouble-shooting tipsand notes on time considerations. Alternative procedures,background information and references supplement the mainprocedures described. Other features include: * Experimental examples to indicate expected results; * Quick reference symbols such as safety icons with warning notes;and, * A list of suppliers is provided to allow easy access tolaboratory products. Written by a team of international scientists, Cell and TissueCulture: Laboratory Procedures in Biotechnology will be of interestto researchers, technicians and process engineers using cellculture within the biotechnology, biomedicine and pharmaceuticalindustries. |
| plant tissue culture lab layout: Pharmacognosy and Phytochemistry Uchenna E. Odoh, Shailendra S. Gurav, Michael O. Chukwuma, 2025-04-08 Key information on plant-based chemical and pharmacology research, from basics and principles through recent technological advances Pharmacognosy and Phytochemistry provides an overview of the basics of pharmacognosy and phytochemistry from early principles through contemporary advances like molecular pharmacognosy. The book covers the classification of crude drugs, complementary and alternative medical (CAM) systems, adulteration and evaluation of drugs, extraction methods of plant drugs, and ethnobotany and ethnopharmacology. The book also reviews the historical overview, therapeutic application, cultural and ecological dimensions of plant-based medicines. Other key chapters discuss biotechnology and clinical pharmacognosy. Written by a group of expert contributors, Pharmacognosy and Phytochemistry reviews sample topics including: Methodologies for extracting bioactive compounds and techniques to perform qualitative and quantitative phytochemical analysis Therapeutic potential of plant secondary metabolites and the processes of isolation, purification, and characterization of herbal drugs Biological screening methods and biosynthetic pathways of phytopharmaceuticals, pharmaceutical aids, nutraceuticals, cosmeceuticals, pesticides, and allergens Comparative phytochemistry, chemotaxonomy, and the emerging field of marine pharmacognosy Combining traditional knowledge with modern advancements to provide a holistic understanding of two important fields, Pharmacognosy and Phytochemistry serves as an excellent resource for students, researchers, and practitioners. |
| plant tissue culture lab layout: Laboratory Design Handbook E. Crawley Cooper, 2014-07-22 Laboratory Design Handbook describes the process, motivation, constraints, challenges, opportunities, and specific design data related to the creation of a modern research laboratory. The information presented is based on a large pool of experience in the development of new and renovated laboratory buildings for universities, teaching hospitals, ph |
| plant tissue culture lab layout: In Vitro Culture of Higher Plants R.L.M Pierik, 1997-06-30 In Vitro Culture of Higher Plants presents an up-to-date and wide- ranging account of the techniques and applications, and has primarily been written in response to practical problems. Special attention has been paid to the educational aspects. Typical methodological aspects are given in the first part: laboratory set-up, composition and preparation of media, sterilization of media and plant material, isolation and (sub)culture, mechanization, the influence of plant and environmental factors on growth and development, the transfer from test-tube to soil, aids to study. The question of why in vitro culture is practised is covered in the second part: embryo culture, germination of orchid seeds, mericloning of orchids, production of disease-free plants, vegetative propagation, somaclonal variation, test-tube fertilization, haploids, genetic manipulation, other applications in phytopathology and plant breeding, secondary metabolites. |
| plant tissue culture lab layout: Tissue Culture in Forestry J.M. Bonga, D.J. Durzan, 2013-04-17 2. IMPORTANCE OF NITROGEN METABOLISM 2. 1. Range of naturally occurring nitrogenous components in forest trees 2. 2. Gene expression and mapping 2. 3. Metabolic changes in organized and unorganized systems 2. 4. Nitrogen and nutrition 2. 5. Aspects of intermediary nitrogen metabolism 3. NITROGEN METABOLISM IN GROWTH AND DEVELOPMENT 3. 1. Precultural factors 3. 2. Callus formation 3. 3. Cell suspensions 3. 3. 1. Conifers 3. 3. 2. Acer 3. 4. Morphogenesis 3. 4. 1. Nitrogen metabolism of natural embryos 3. 4. 2. Somatic embryogenesis 3. 4. 2. 1. Sweetgum (Liquidambar styraciflua) 3. 4. 2. 2. Douglar-fir and loblolly pine 3. 4. 3. Organogenesis 4. OUTLOOK 11. CARBOHYDRATE UTILIZATION AND METABOLISM - T. A. Thorpe 325 1. INTRODUCTION 2. NUTRITIONAL ASPECTS 3. CARBOHYDRATE UPTAKE 4. CARBOHYDRATE METABOLISM 4. 1. Sucrose degradation 4. 2. Metabolism of other carbon sources 4. 3. Hexose mobilization and metabolism 4. 3. 1. Cell cycle studies 4. 3. 2. Growth studies 4. 3. 3. Organized development 4. 4. Cell wall biogenesis 4. 4. 1. Primary cell walls 4. 4. 2. Cell wall turnover 4. 4. 3. Secondary cell walls 4. 5. Carbon skeleton utilization 5. OSMOTIC ROLE 6. CONCLUDING THOUGHTS 369 12. THE USE OF IN VITRO TECHNIQUES FOR GENETIC MODIFICATIO~FOREST TREES - E. G. Kirby 1. INTRODUCTION 2. IN VITRO SELECTION 2. 1. Natural variation 2. 2. Induction of variation 2. 3. Selection techniques 2. 4. Plant regeneration 2 . • 5. Applications x 3. SOMATIC HYBRIDIZATION 3. 1. |
| plant tissue culture lab layout: In Vitro Culture of Trees J.M. Bonga, Patrick Aderkas, 1992-05-31 Laboratory organization and equipment. Media preparation. Collection, sterilization, excision and culture. Clonal propagation. Special cultures. Secondary metabolite production. Nitrogen fixation. Storage of cultures. Genetic modification. |
| plant tissue culture lab layout: Experiments in Plant Tissue Culture John H. Dodds, Lorin W. Roberts, 1985-10-31 The second edition of Experiments in Plant Tissue Culture makes available new information that has resulted from recent advances in the applications of plant tissue culture techniques to agriculture and industry. This comprehensive laboratory text takes the reader through a graded series of experimental protocols and also provides an introductory review of each topic. Topics include: a plant tissue culture laboratory, aseptic techniques, nutritional components of media, callus induction, organ formation, xylem cell differentiation, root cultures, cell suspensions, micropropagation, embryogenesis, isolation and fusion of protoplasts, haploid cultures, storage of plant genetic resources, secondary metabolite production, and quantification of procedures. This volume offers all of the basic experimental methods for the major research areas of plant tissue culture, and it will be invaluable to undergraduates and research investigators in the plant sciences. |
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Apr 9, 2020 · On this page you’ll find a complete guide to plants and the plant kingdom. You’ll discover what makes a plant a plant, why plants are important, the different types of plant and …
What is a plant? — Science Learning Hub
The word ‘plant’ encompasses a wide range of living organisms, all of which belong to the kingdom Plantae and share a range of characteristics. In this article, we examine the key …
Plant Definition and Examples - Biology Online Dictionary
May 14, 2024 · Plants are eukaryotic organisms that are photosynthetic and with a rigid cell wall. Learn the definition of a plant, its structure, taxonomic groups, characteristics, evolution, and …
Types of Plants: Different Kinds of Plants and Their Classification
Jun 12, 2020 · Discover different types of plants and plant groups, such as flowering plants, ornamental grasses, ferns and more.
Plant - Wikipedia
Plants are the eukaryotes that form the kingdom Plantae; they are predominantly photosynthetic. This means that they obtain their energy from sunlight, using chloroplasts derived from …
Plant | Definition, Evolution, Ecology, & Taxonomy | Britannica
May 26, 2025 · Plant, any multicellular, eukaryotic, usually photosynthetic life-form in the kingdom Plantae. There are an estimated 390,900 different species of plants known to science. Learn …
Plant - Definition, Characteristics and Types | Biology Dictionary
Apr 28, 2017 · Plant Definition. Plants are multicellular organisms in the kingdom Plantae that use photosynthesis to make their own food. There are over 300,000 species of plants; common …
Plant - Simple English Wikipedia, the free encyclopedia
Plants include familiar types such as trees, herbs, bushes, grasses, vines, ferns, mosses, and green algae. The scientific study of plants, known as botany, has identified about 391,000 extant …
NYFA: New York Flora Atlas
May 23, 2025 · The New York Flora Atlas is a source of information for the distribution of plants within the state, as well as information on plant habitats, associated ecological communities, and …
Plants - NYSDEC - New York State Department of Environmental …
New York State's many habitats, both on land and water, shelter rich and diverse plant life. From the beloved sugar maple to the common milkweed, both humans and wildlife depend on the wide …
Plants: The Ultimate Guide To The Plant Kingdom, With Pictures …
Apr 9, 2020 · On this page you’ll find a complete guide to plants and the plant kingdom. You’ll discover what makes a plant a plant, why plants are important, the different types of plant and …
What is a plant? — Science Learning Hub
The word ‘plant’ encompasses a wide range of living organisms, all of which belong to the kingdom Plantae and share a range of characteristics. In this article, we examine the key characteristics of …
Plant Definition and Examples - Biology Online Dictionary
May 14, 2024 · Plants are eukaryotic organisms that are photosynthetic and with a rigid cell wall. Learn the definition of a plant, its structure, taxonomic groups, characteristics, evolution, and …
Types of Plants: Different Kinds of Plants and Their Classification
Jun 12, 2020 · Discover different types of plants and plant groups, such as flowering plants, ornamental grasses, ferns and more.
