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| methods in yeast genetics a laboratory course manual: Methods in Yeast Genetics David C. Amberg, Dan Burke, Jeffrey N. Strathern, 2005 Methods in Yeast Genetics is a course that has been offered annually at Cold Spring Harbor for the last 30 years. This provides a set of teaching experiments along with the protocols and recipes for the standard techniques and reagents used in the study of yeast biology. |
| methods in yeast genetics a laboratory course manual: Methods in Yeast Genetics Chris Kaiser, Susan Michaelis, Aaron Mitchell, 1994 |
| methods in yeast genetics a laboratory course manual: Methods in Yeast Genetics , 1998 |
| methods in yeast genetics a laboratory course manual: Methods in Yeast Genetics Fred Sherman, Gerald Fink, James Hicks, 1987-04-01 |
| methods in yeast genetics a laboratory course manual: Methods in Yeast Genetics and Genomics Maitreya J. Dunham, Marc R. Gartenberg, Grant W. Brown, 2015 Methods in Yeast Genetics is a course that has been offered annually at Cold Spring Harbor Laboratory for the last 45 years. This is an updated edition of the course manual, which provides a set of teaching experiments, along with protocols and recipes for the standard techniques and reagents used in the study of yeast biology. Since the last edition of the manual was published (2005), revolutionary advances in genomics, proteomics, and imaging technologies have had a significant impact on the field. The 11 experiments included in this manual provide a foundation of methods for any modern-day yeast lab. These methods emphasize combinations of classical and modern genetic approaches, including isolation and characterization of mutants, two-hybrid analysis, tetrad analysis, complementation, and recombination. Also covered are molecular genetic techniques for genome engineering. Additional experiments introduce fundamental techniques in yeast genomics, including both performance and interpretation of Synthetic Genetic Array analysis, multiplexed whole genome and barcode sequencing, and comparative genomic hybridization to DNA arrays. Comparative genomics is introduced using different yeast strains to study natural variation, evolution, and quantitative traits. This manual covers the full repertoire of genetic approaches needed to dissect complex biological problems in the yeast Saccharomyces cerevisiae. |
| methods in yeast genetics a laboratory course manual: Methods in Yeast Genetics Mark David Rose, Fred Marshall Winston, Philip Hieter, 1990 An intensive course in yeast genetics has been held at Cold Spring Harbor Laboratory for some years, and the course manual reflects its content and scope. Since its last publication in 1987, this manual's sequence of experiments has been extensively updated and expanded, and the protocols and append |
| methods in yeast genetics a laboratory course manual: Methods in Yeast Genetics , 1972 |
| methods in yeast genetics a laboratory course manual: Methods in Yeast Genetics Chris Kaiser, Susan Michaelis, Aaron Mitchell, 1994-01-01 |
| methods in yeast genetics a laboratory course manual: Fission Yeast Iain Hagan, Antony M. Carr, Agnes Grallert, Paul Nurse, 2016 Fission yeast are unicellular, rod-shaped fungi that divide by medial fission. Studies using fission yeast were instrumental in identifying fundamental mechanisms that govern cell division, differentiation, and epigenetics, to name but a few. Their rapid growth rate, genetic malleability, and similarities to more complex eukaryotes continue to make them excellent subjects for many biochemical, molecular, and cell biological studies. This laboratory manual provides an authoritative collection of core experimental procedures that underpin modern fission yeast research. The contributors describe basic methods for culturing and genetically manipulating fission yeast, synchronization strategies for probing the cell cycle, technologies for assessing proteins, metabolites, and cell wall constituents, imaging methods to visualize subcellular structures and dynamics, and protocols for investigating chromatin and nucleic acid metabolism. Modifications to techniques commonly used in related species (e.g., budding yeast) are noted, as are useful resources for fission yeast researchers, including various databases and repositories. The well-studied fission yeast Schizosaccharomyces pombe is the focus throughout, but the emerging model S. japonicus-a larger, dimorphic species with several desirable characteristics-is also covered. This manual is an important reference for existing fission yeast laboratories and will serve as an essential start-up guide for those working with fission yeast for the first time. |
| methods in yeast genetics a laboratory course manual: Methods in Yeast Genetics Cold Spring Harbor Laboratory of Quantitative Biology, Fred Sherman, Gerald R. Fink, Christopher W. Lawrence, 1974 |
| methods in yeast genetics a laboratory course manual: Guide to Yeast Genetics and Molecular and Cell Biology, Part C , 2002-06-14 This volume and its companion, Volume 350, are specifically designed to meet the needs of graduate students and postdoctoral students as well as researchers, by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable newcomers to set up a yeast laboratory and to master basic manipulations. Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines. Specific topics addressed in this book include cytology, biochemistry, cell fractionation, and cell biology. |
| methods in yeast genetics a laboratory course manual: Yeast Biotechnology: Diversity and Applications T. Satyanarayana, Gotthard Kunze, 2009-04-24 I belie ve that the book would provide an overview of the recent developments in the domain of yeast research with some new ideas, which could serve as an inspiration and challenge for researchers in this field. Ne w Delhi Prof. Asis Datta Dec. 24, 2007 F ormer Vice-chancellor, JNU Director, NCPGR (New Delhi) Pr eface Yeasts are eukaryotic unicellular microfungi that are widely distributed in the natural environments. Although yeasts are not as ubiquitous as bacteria in the na- ral environments, they have been isolated from terrestrial, aquatic and atmospheric environments. Yeast communities have been found in association with plants, a- mals and insects. Several species of yeasts have also been isolated from specialized or extreme environments like those with low water potential (e. g. high sugar/salt concentrations), low temperature (e. g. yeasts isolated from Antarctica), and low oxygen availability (e. g. intestinal tracts of animals). Around 1500 species of yeasts belonging to over 100 genera have been described so far. It is estimated that only 1% of the extant yeasts on earth have been described till date. Therefore, global efforts are underway to recover new yeast species from a variety of normal and extreme environments. Yeasts play an important role in food chains, and carbon, nitrogen and sulphur cycles. Yeasts can be genetically manipulated by hybridization, mutation, rare m- ing, cytoduction, spheroplast fusion, single chromosomal transfer and transfor- tion using recombinant technology. Yeasts (e. g. |
| methods in yeast genetics a laboratory course manual: Phage Display Carlos F. Barbas, 2001 Phage-display technology has begun to make critical contributions to the study of molecular recognition. DNA sequences are cloned into phage, which then present on their surface the proteins encoded by the DNA. Individual phage are rescued through interaction of the displayed protein with a ligand, and the specific phage is amplified by infection of bacteria. Phage-display technology is powerful but challenging and the aim of this manual is to provide comprehensive instruction in its theoretical and applied so that any scientist with even modest molecular biology experience can effectively employ it. The manual reflects nearly a decade of experience with students of greatly varying technical expertise andexperience who attended a course on the technology at Cold Spring Harbor Laboratory. Phage-display technology is growing in importance and power. This manual is an unrivalled source of expertise in its execution and application. |
| methods in yeast genetics a laboratory course manual: Methods in Yeast Genetics , 1983 |
| methods in yeast genetics a laboratory course manual: Molecular Biology Techniques Walt Ream, Katharine G. Field, 1998-11-17 This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. - Provides well-tested experimental protocols for each technique - Lists the reagents and preparation of each experiment separately - Contains a complete schedule of experiments and the preparation required - Includes study questions at the end of each chapter |
| methods in yeast genetics a laboratory course manual: Budding Yeast Brenda Andrews, Charles M. Boone, Trisha Davis, Stanley Fields, 2016 Over the past century, studies of the budding yeast Saccharomyces cerevisiae have helped to unravel principles of nearly every aspect of eukaryotic cell biologyfrom metabolism and molecular genetics to cell division and differentiation. Thanks to its short generation time, ease of genetic manipulation, and suitability for high-throughput studies, yeast remains the focus of research in a vast number of laboratories worldwide. This laboratory manual provides a comprehensive collection of experimental procedures that continue to make budding yeast an informative model. The contributors describe methods for culturing and genetically modifying yeast, strategies and tools (e.g., gene deletion collections) for functional analyses, approaches for characterizing cell structure and morphology, and techniques to probe the modifications and interactions of various cellular constituents (e.g., using one- and two-hybrid screens). Strategies for studying metabolomics, complex traits, and evolution in yeast are also covered, as are methods to isolate and investigate new strains of yeast from the wild. Several additional chapters are devoted to bioinformatics tools and resources for yeast biology (e.g., the Saccharomyces Genome Database). This manual is therefore an essential resource for all researchers, from graduate level upward, who use budding yeast to explore the intricate workings of cells. |
| methods in yeast genetics a laboratory course manual: Laboratory Course Manual for Methods in Yeast Genetics Fred Sherman, Gerald R. Fink, James B. Hicks, 1986 |
| methods in yeast genetics a laboratory course manual: Early Development of Xenopus Laevis Hazel L. Sive, Robert M. Grainger, Richard M. Harland, 2000 Amphibian embryos are supremely valuable in studies of early vertebrate development because they are large, handle easily, and can be obtained at many interesting stages. And of all the amphibians available for study, the most valuable is Xenopus laevis,which is easy to keep and ovulates at any time of year in response to simple hormone injections. Xenopusembryos have been studied for years but this is a particularly exciting time for the field. Techniques have become available very recently that permit a previously impossible degree of manipulation of gene expression in intact embryos, as well as the ability to visualize the results of such manipulation. As a result, a sophisticated new understanding of Xenopusdevelopment has emerged, which ensures the species’ continued prominent position among the organisms favored for biological investigation. This manual contains a comprehensive collection of protocols for the study of early development in Xenopusembryos. It is written by several of the field’s most prominent investigators in the light of the experience they gained as instructors in an intensive laboratory course taught at Cold Spring Harbor Laboratory since 1991. As a result it contains pointers, hints, and other technical knowledge not readily available elsewhere. This volume is essential reading for all investigators interested in the developmental and cell biology of Xenopusand vertebrates generally. Many of the techniques described here are illustrated in an accompanying set of videotapeswhich are cross-referenced to the appropriate section of the manual. |
| methods in yeast genetics a laboratory course manual: Methods in Yeast Genetics. A Laboratory Course Manual. Instructors , 1990 |
| methods in yeast genetics a laboratory course manual: Methods in Yeast Genetics Gerald R. Fink, James B. Hicks, 1981 |
| methods in yeast genetics a laboratory course manual: Experiments in Molecular Genetics Jeffrey H. Miller, Cold Spring Harbor Laboratory, 1974 |
| methods in yeast genetics a laboratory course manual: DNA Science David A. Micklos, Greg A. Freyer, David A. Crotty, 2003 This is the second edition of a highly successful textbook (over 50,000 copies sold) in which a highly illustrated, narrative text is combined with easy–to–use thoroughly reliable laboratory protocols. It contains a fully up–to–date collection of 12 rigorously tested and reliable lab experiments in molecular biology, developed at the internationally renowned Dolan DNA Learning Center of Cold Spring Harbor Laboratory, which culminate in the construction and cloning of a recombinant DNA molecule. Proven through more than 10 years of teaching at research and nonresearch colleges and universities, junior colleges, community colleges, and advanced biology programs in high school, this book has been successfully integrated into introductory biology, general biology, genetics, microbiology, cell biology, molecular genetics, and molecular biology courses. The first eight chapters have been completely revised, extensively rewritten, and updated. The new coverage extends to the completion of the draft sequence of the human genome and the enormous impact these and other sequence data are having on medicine, research, and our view of human evolution. All sections on the concepts and techniques of molecular biology have been updated to reflect the current state of laboratory research. The laboratory experiments cover basic techniques of gene isolation and analysis, honed by over 10 years of classroom use to be thoroughly reliable, even in the hands of teachers and students with no prior experience. Extensive prelab notes at the beginning of each experiment explain how to schedule and prepare, while flow charts and icons make the protocols easy to follow. As in the first edition of this book, the laboratory course is completely supported by quality–assured products from the Carolina Biological Supply Company, from bulk reagents, to useable reagent systems, to single–use kits, thus satisfying a broad range of teaching applications. |
| methods in yeast genetics a laboratory course manual: Proteomics Andrew J. Link, Joshua LaBaer, 2009 Based on a popular course at Cold Spring Harbor Laboratory, this new manual assembles cutting-edge protocols, helpful hints, and lecture notes to teach researchers from a wide variety of disciplines the essential methods of proteomics using state-of-the-art instrumentation. Detailed protocols involving protein microarrays, liquid chromatography, high-throughput cloning of expression constructs, IMAC, mass spectrometry, MALDI–TOF, and MudPIT are provided, along with well-illustrated descriptions of experimental procedures and lists of recommended Web sites and reading material. Proteomics: A Cold Spring Harbor Laboratory Course Manualcan be used both as the basis for a course and as a detailed bench manual for those performing indispensable proteomic experiments. It is authored by Andrew J. Link and Joshua LaBaer, both leaders in their fields, who bring complementary expertise to the manual. |
| methods in yeast genetics a laboratory course manual: Techniques in Genetic Engineering Isil Aksan Kurnaz, 2015-05-08 Although designed for undergraduates with an interest in molecular biology, biotechnology, and bioengineering, this book-Techniques in Genetic Engineering-IS NOT: a laboratory manual; nor is it a textbook on molecular biology or biochemistry. There is some basic information in the appendices about core concepts such as DNA, RNA, protein, genes, and |
| methods in yeast genetics a laboratory course manual: Drosophila Neurobiology , 2024-10 |
| methods in yeast genetics a laboratory course manual: Handbook of Statistical Genetics David J. Balding, Martin Bishop, Chris Cannings, 2008-06-10 The Handbook for Statistical Genetics is widely regarded as the reference work in the field. However, the field has developed considerably over the past three years. In particular the modeling of genetic networks has advanced considerably via the evolution of microarray analysis. As a consequence the 3rd edition of the handbook contains a much expanded section on Network Modeling, including 5 new chapters covering metabolic networks, graphical modeling and inference and simulation of pedigrees and genealogies. Other chapters new to the 3rd edition include Human Population Genetics, Genome-wide Association Studies, Family-based Association Studies, Pharmacogenetics, Epigenetics, Ethic and Insurance. As with the second Edition, the Handbook includes a glossary of terms, acronyms and abbreviations, and features extensive cross-referencing between the chapters, tying the different areas together. With heavy use of up-to-date examples, real-life case studies and references to web-based resources, this continues to be must-have reference in a vital area of research. Edited by the leading international authorities in the field. David Balding - Department of Epidemiology & Public Health, Imperial College An advisor for our Probability & Statistics series, Professor Balding is also a previous Wiley author, having written Weight-of-Evidence for Forensic DNA Profiles, as well as having edited the two previous editions of HSG. With over 20 years teaching experience, he’s also had dozens of articles published in numerous international journals. Martin Bishop – Head of the Bioinformatics Division at the HGMP Resource Centre As well as the first two editions of HSG, Dr Bishop has edited a number of introductory books on the application of informatics to molecular biology and genetics. He is the Associate Editor of the journal Bioinformatics and Managing Editor of Briefings in Bioinformatics. Chris Cannings – Division of Genomic Medicine, University of Sheffield With over 40 years teaching in the area, Professor Cannings has published over 100 papers and is on the editorial board of many related journals. Co-editor of the two previous editions of HSG, he also authored a book on this topic. |
| methods in yeast genetics a laboratory course manual: Nonconventional Yeasts in Biotechnology Klaus Wolf, 2012-12-06 Nonconventional yeasts - all yeasts other than S. cerevisiae and S. pombe - are attracting increasing attention in basic research and biotechnological applications. Due to their exceptional metabolic pathways, they have been used in various biotechnological processes for producing foods or food additives, drugs or a variety of biochemicals. This book is the first to extensively cover nonconventional yeasts. In addition to useful background information detailed protocols are included, allowing investigation of basic and applied aspects of a wide range of nonconventional yeast species. |
| methods in yeast genetics a laboratory course manual: Laboratory Manual for a course Methods in yeast genetics , 1972 |
| methods in yeast genetics a laboratory course manual: Yeast technology Gerald Reed, 2012-12-06 Yeasts are the active agents responsible for three of our most important foods - bread, wine, and beer - and for the almost universally used mind/ personality-altering drug, ethanol. Anthropologists have suggested that it was the production of ethanol that motivated primitive people to settle down and become farmers. The Earth is thought to be about 4. 5 billion years old. Fossil microorganisms have been found in Earth rock 3. 3 to 3. 5 billion years old. Microbes have been on Earth for that length of time carrying out their principal task of recycling organic matter as they still do today. Yeasts have most likely been on Earth for at least 2 billion years before humans arrived, and they playa key role in the conversion of sugars to alcohol and carbon dioxide. Early humans had no concept of either microorganisms or fermentation, yet the earliest historical records indicate that by 6000 B. C. they knew how to make bread, beer, and wine. Earliest humans were foragers who col lected andate leaves, tubers, fruits, berries, nuts, and cereal seeds most of the day much as apes do today in the wild. Crushed fruits readily undergo natural fermentation by indigenous yeasts, and moist seeds germinate and develop amylases that produce fermentable sugars. Honey, the first con centrated sweet known to humans, also spontaneously ferments to alcohol if it is by chance diluted with rainwater. Thus, yeasts and other microbes have had a long history of 2 to 3. |
| methods in yeast genetics a laboratory course manual: The AGT Cytogenetics Laboratory Manual Marilyn S. Arsham, Margaret J. Barch, Helen J. Lawce, 2017-04-24 Cytogenetics is the study of chromosome morphology, structure, pathology, function, and behavior. The field has evolved to embrace molecular cytogenetic changes, now termed cytogenomics. Cytogeneticists utilize an assortment of procedures to investigate the full complement of chromosomes and/or a targeted region within a specific chromosome in metaphase or interphase. Tools include routine analysis of G-banded chromosomes, specialized stains that address specific chromosomal structures, and molecular probes, such as fluorescence in situ hybridization (FISH) and chromosome microarray analysis, which employ a variety of methods to highlight a region as small as a single, specific genetic sequence under investigation. The AGT Cytogenetics Laboratory Manual, Fourth Edition offers a comprehensive description of the diagnostic tests offered by the clinical laboratory and explains the science behind them. One of the most valuable assets is its rich compilation of laboratory-tested protocols currently being used in leading laboratories, along with practical advice for nearly every area of interest to cytogeneticists. In addition to covering essential topics that have been the backbone of cytogenetics for over 60 years, such as the basic components of a cell, use of a microscope, human tissue processing for cytogenetic analysis (prenatal, constitutional, and neoplastic), laboratory safety, and the mechanisms behind chromosome rearrangement and aneuploidy, this edition introduces new and expanded chapters by experts in the field. Some of these new topics include a unique collection of chromosome heteromorphisms; clinical examples of genomic imprinting; an example-driven overview of chromosomal microarray; mathematics specifically geared for the cytogeneticist; usage of ISCN’s cytogenetic language to describe chromosome changes; tips for laboratory management; examples of laboratory information systems; a collection of internet and library resources; and a special chapter on animal chromosomes for the research and zoo cytogeneticist. The range of topics is thus broad yet comprehensive, offering the student a resource that teaches the procedures performed in the cytogenetics laboratory environment, and the laboratory professional with a peer-reviewed reference that explores the basis of each of these procedures. This makes it a useful resource for researchers, clinicians, and lab professionals, as well as students in a university or medical school setting. |
| methods in yeast genetics a laboratory course manual: An Introduction to Genetic Engineering Desmond S. T. Nicholl, 2002-02-07 The author presents a basic introduction to the world of genetic engineering. Copyright © Libri GmbH. All rights reserved. |
| methods in yeast genetics a laboratory course manual: Manual of Medical Laboratory Techniques S Ramakrishnan, KN Sulochana, 2012-12-15 This is the 1st edition of the book Manual of Medical Laboratory Techniques. The text is comprehensive, updated and fully revised as per the present day requirements in the subject of medical laboratory technique. In this book principles, methodologies, results norms, interpretations diseases concerned and bibliography are included for each test. The book has 5 chapters. The first chapter deals with biochemical tests. Chapter two provides a comprehensive description of tests done for genetic analysis. A sound foundation of understanding of test in hematology, microbiology and serology is provided in next 2 chapters. Chapter 5th, deals with ophthalmic histopathology. A comprehensive index is given at last. |
| methods in yeast genetics a laboratory course manual: Manipulating the Mouse Embryo Brigid Hogan, Frank Costantini, Elizabeth Lacy, 1986 Of mouse development -- Setting up a colony for the production of transgenic mice -- Recovery, culture, and transfer of embryos -- Introduction of new genetic information into the developing mouse embryo -- Iolation of pluripotential stem cell lines -- Techhniques for visualizing genes and gene products -- In vitro culture of eggs, embryos, and teratocarcinoma cels -- Chemicals, supplies, and solutions. |
| methods in yeast genetics a laboratory course manual: Yeast Genetics Jeffrey S. Smith, Daniel J. Burke, 2016-09-17 Yeast Genetics: Methods and Protocols is a collection of methods to best study and manipulate Saccharomyces cerevisiae, a truly genetic powerhouse. The simple nature of a single cell eukaryotic organism, the relative ease of manipulating its genome and the ability to interchangeably exist in both haploid and diploid states have always made it an attractive model organism. Genes can be deleted, mutated, engineered and tagged at will. Saccharomyces cerevisiae has played a major role in the elucidation of multiple conserved cellular processes including MAP kinase signaling, splicing, transcription and many others. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Yeast Genetics: Methods and Protocols will provide a balanced blend of classic and more modern genetic methods relevant to a wide range of research areas and should be widely used as a reference in yeast labs. |
| methods in yeast genetics a laboratory course manual: Methods in Yeast Genetics Fred Sherman, 1979 |
| methods in yeast genetics a laboratory course manual: Basic Techniques in Molecular Biology Stefan Surzycki, 2012-12-06 This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here. |
| methods in yeast genetics a laboratory course manual: Gene Synthesis Jean Peccoud, 2012-02-10 The de novo fabrication of custom DNA molecules is a transformative technology that significantly affects the biotechnology industry. Basic genetic engineering techniques for manipulating DNA in vitro opened an incredible field of opportunity in the life sciences. In, Gene Synthesis: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to fabricate DNA . These include methods and techniques for the assembly of oligonucleotide, cloning of synthons into larger fragments, protocols and software applications, and educational and biosecurity impacts of gene synthesis. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Thorough and intuitive, Gene Synthese: Methods and Protocols aids scientists in understanding all the different stages of a complex gene synthesis process, while refining their understanding of gene synthesis and determine what part of the process they can or should do in their laboratory and what parts should be contracted to a specialized service provider. |
| methods in yeast genetics a laboratory course manual: Methods in Yeast Genetics Fred Sherman, 1981 |
| methods in yeast genetics a laboratory course manual: Genetics and Biology of Alcoholism C. Robert Cloninger, Henri Begleiter, 1990 These proceedings from the conference of Fall 1989, evaluate the obstacles and challenges of searching for the specific genes influencing risk of alcoholism. Coverage includes genetic and environmental risk factors, neurobiological markers of risk, animal models and candidate genes for alcoholism, a |
| methods in yeast genetics a laboratory course manual: Methods in Yeast Genetics. (Laboratory Manual). Course Instructors , 1981 |
Methods - University of Nebraska Medical Center
Methods Mol. Biol. 416:103-116. Fey PD, Endres JL, Yajjala VK, Widhelm TJ, Boissy RJ, Bose JL, and Bayles KW. 2013. A …
Tn Insertion Map
Please note that because the NTML website has been updated to the S. aureus JE2 genome, the transposon …
Tools - University of Nebraska Medical Center
Methods Tools Contact The NTML Screening Array The Nebraska Transposon Mutant Library (NTML) …
Contact - ntml.unmc.edu
Methods Tools Contact Center for Staphylococcal Research (CSR) Department of Pathology & …
Stats - University of Nebraska Medical Center
Staphylococcus aureus subsp. aureus USA300_FPR3757 GenBank RefSeq features: Feature type Count Count …
Methods - University of Nebraska Medical Center
Methods Mol. Biol. 416:103-116. Fey PD, Endres JL, Yajjala VK, Widhelm TJ, Boissy RJ, Bose JL, and Bayles KW. 2013. A genetic resource for rapid and comprehensive phenotype screening of …
Tn Insertion Map
Please note that because the NTML website has been updated to the S. aureus JE2 genome, the transposon insertion site location numbers now reflect the JE2 genome numbering.
Tools - University of Nebraska Medical Center
Methods Tools Contact The NTML Screening Array The Nebraska Transposon Mutant Library (NTML) Screening Array for phenotype screens consists of 1,920 transposon (Tn) mutants of the …
Contact - ntml.unmc.edu
Methods Tools Contact Center for Staphylococcal Research (CSR) Department of Pathology & Microbiology University of Nebraska Medical Center 985900 Nebraska Medical Center Omaha, …
Stats - University of Nebraska Medical Center
Staphylococcus aureus subsp. aureus USA300_FPR3757 GenBank RefSeq features: Feature type Count Count % Length (bp, total) Length % [Genome] 2,872,769
EcoRI (2) TN up eqFP650 TIR oriV TN down
pFP650-R 8773 bp Ampr Cmr eqFP650 Counter selection TN up TN down TIR pE194ts oriV EcoRI (2) SalI(1793) E. coli Features -oriV: high copy origin -Ampr: Ampicillin resistance (100 μg ml-1) …
EcoRI (2) TN up TIR r eqFP650 oriV TN down - University of …
pFP650-F 8773 bp Ampr Cmr eqFP650 Counter selection TN up TN down TIR pE194ts oriV EcoRI (2) SalI(1793) E. coli Features -oriV: high copy origin -Ampr: Ampicillin resistance (100 μg ml-1) …
