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| methods in molecular biophysics: Methods in Molecular Biophysics Nathan R. Zaccai, Igor N. Serdyuk, Joseph Zaccai, 2017-05-18 Current techniques for studying biological macromolecules and their interactions are based on the application of physical methods, ranging from classical thermodynamics to more recently developed techniques for the detection and manipulation of single molecules. Reflecting the advances made in biophysics research over the past decade, and now including a new section on medical imaging, this new edition describes the physical methods used in modern biology. All key techniques are covered, including mass spectrometry, hydrodynamics, microscopy and imaging, diffraction and spectroscopy, electron microscopy, molecular dynamics simulations and nuclear magnetic resonance. Each method is explained in detail using examples of real-world applications. Short asides are provided throughout to ensure that explanations are accessible to life scientists, physicists and those with medical backgrounds. The book remains an unparalleled and comprehensive resource for graduate students of biophysics and medical physics in science and medical schools, as well as for research scientists looking for an introduction to techniques from across this interdisciplinary field. |
| methods in molecular biophysics: Methods in Molecular Biophysics Nathan R. Zaccai, Giuseppe Zaccai, Igor N. Serdyuk, 2017 |
| methods in molecular biophysics: Methods in Molecular Biophysics Igor N. Serdyuk, 2007 Advanced textbook describing the key physical methods used in molecular biophysics. |
| methods in molecular biophysics: Introduction to Experimental Biophysics Jay L. Nadeau, 2016-04-19 Increasing numbers of physicists, chemists, and mathematicians are moving into biology, reading literature across disciplines, and mastering novel biochemical concepts. To succeed in this transition, researchers must understand on a practical level what is experimentally feasible. The number of experimental techniques in biology is vast and often s |
| methods in molecular biophysics: Methods in Molecular Biophysics Igor N. Serdyuk, Nathan R. Zaccai, Joseph Zaccai, 2007-03-29 Our knowledge of biological macromolecules and their interactions is based on the application of physical methods, ranging from classical thermodynamics to recently developed techniques for the detection and manipulation of single molecules. These methods, which include mass spectrometry, hydrodynamics, microscopy, diffraction and crystallography, electron microscopy, molecular dynamics simulations, and nuclear magnetic resonance, are complementary; each has its specific advantages and limitations. Organised by method, this textbook provides descriptions and examples of applications for the key physical methods in modern biology. It is an invaluable resource for undergraduate and graduate students of molecular biophysics in science and medical schools, as well as research scientists looking for an introduction to techniques beyond their specialty. As appropriate for this interdisciplinary field, the book includes short asides to explain physics aspects to biologists and biology aspects to physicists. |
| methods in molecular biophysics: Methods in Molecular biophysics Mr. Rohit Manglik, 2024-07-08 EduGorilla Publication is a trusted name in the education sector, committed to empowering learners with high-quality study materials and resources. Specializing in competitive exams and academic support, EduGorilla provides comprehensive and well-structured content tailored to meet the needs of students across various streams and levels. |
| methods in molecular biophysics: Molecular Biophysics for the Life Sciences Norma Allewell, Linda O. Narhi, Ivan Rayment, 2013-09-28 This volume provides an overview of the development and scope of molecular biophysics and in-depth discussions of the major experimental methods that enable biological macromolecules to be studied at atomic resolution. It also reviews the physical chemical concepts that are needed to interpret the experimental results and to understand how the structure, dynamics, and physical properties of biological macromolecules enable them to perform their biological functions. Reviews of research on three disparate biomolecular machines—DNA helicases, ATP synthases, and myosin--illustrate how the combination of theory and experiment leads to new insights and new questions. |
| methods in molecular biophysics: Methods in Modern Biophysics Bengt Nölting, 2013-03-09 Incorporating dramatic recent advances, this textbook presents a fresh and timely introduction to modern biophysical methods. An array of new, faster and structurally higher-resolving power biophysical methods now enables scientists to examine the examination of the mysteries of life at a molecular level. So students and researchers alike need to know the technological details behind the latest methods so they can choose appropriate tools and make optimal use of them. This innovative text surveys and explains the ten key biophysical methods, including those related to biophysical nanotechnology, scanning probe microscopy, X-ray crystallography, ion mobility spectrometry, mass spectrometry, and proteomics. Containing much information previously unavailable in tutorial form, Methods in Modern Biophysics employs worked examples and more than 260 illustrations to fully detail the techniques and their underlying mechanisms. The book was written for advanced undergraduateand graduate students, postdocs, researchers, lecturers and professors in biophysics, biochemistry, general biology and related fields. |
| methods in molecular biophysics: Methods in Molecular Biophysics Igor N. Serdyuk, Nathan R. Zaccai, Joseph Zaccai, 2007-03-29 Our knowledge of biological macromolecules and their interactions is based on the application of physical methods, ranging from classical thermodynamics to recently developed techniques for the detection and manipulation of single molecules. These methods, which include mass spectrometry, hydrodynamics, microscopy, diffraction and crystallography, electron microscopy, molecular dynamics simulations, and nuclear magnetic resonance, are complementary; each has its specific advantages and limitations. Organised by method, this textbook provides descriptions and examples of applications for the key physical methods in modern biology. It is an invaluable resource for undergraduate and graduate students of molecular biophysics in science and medical schools, as well as research scientists looking for an introduction to techniques beyond their specialty. As appropriate for this interdisciplinary field, the book includes short asides to explain physics aspects to biologists and biology aspects to physicists. |
| methods in molecular biophysics: Integrated Molecular and Cellular Biophysics Valerica Raicu, Aurel Popescu, 2008-06-17 Biophysics represents perhaps one of the best examples of interdisciplinary research areas, where concepts and methods from disciplines such as physics, biology, b- chemistry, colloid chemistry, and physiology are integrated. It is by no means a new ?eld of study and has actually been around, initially as quantitative physiology and partly as colloid science, for over a hundred years. For a long time, biophysics has been taught and practiced as a research discipline mostly in medical schools and life sciences departments, and excellent biophysics textbooks have been published that are targeted at a biologically literate audience. With a few exceptions, it is only relatively recently that biophysics has started to be recognized as a physical science and integrated into physics departments’ curr- ula, sometimes under the new name of biological physics. In this period of cryst- lization and possible rede?nition of biophysics, there still exists some uncertainty as to what biophysics might actually represent. A particular tendency among phy- cists is to associate biophysics research with the development of powerful new te- niques that should eventually be used not by physicists to study physical processes in living matter, but by biologists in their biological investigations. There is value in that judgment, and excellent books have been published that introduce the int- ested reader to the use of physical principles for the development of new methods of investigation in life sciences. |
| methods in molecular biophysics: Comprehensive Biophysics , 2012-04-12 Biophysics is a rapidly-evolving interdisciplinary science that applies theories and methods of the physical sciences to questions of biology. Biophysics encompasses many disciplines, including physics, chemistry, mathematics, biology, biochemistry, medicine, pharmacology, physiology, and neuroscience, and it is essential that scientists working in these varied fields are able to understand each other's research. Comprehensive Biophysics, Nine Volume Set will help bridge that communication gap. Written by a team of researchers at the forefront of their respective fields, under the guidance of Chief Editor Edward Egelman, Comprehensive Biophysics, Nine Volume Set provides definitive introductions to a broad array of topics, uniting different areas of biophysics research - from the physical techniques for studying macromolecular structure to protein folding, muscle and molecular motors, cell biophysics, bioenergetics and more. The result is this comprehensive scientific resource - a valuable tool both for helping researchers come to grips quickly with material from related biophysics fields outside their areas of expertise, and for reinforcing their existing knowledge. Biophysical research today encompasses many areas of biology. These studies do not necessarily share a unique identifying factor. This work unites the different areas of research and allows users, regardless of their background, to navigate through the most essential concepts with ease, saving them time and vastly improving their understanding The field of biophysics counts several journals that are directly and indirectly concerned with the field. There is no reference work that encompasses the entire field and unites the different areas of research through deep foundational reviews. Comprehensive Biophysics fills this vacuum, being a definitive work on biophysics. It will help users apply context to the diverse journal literature offering, and aid them in identifying areas for further research Chief Editor Edward Egelman (E-I-C, Biophysical Journal) has assembled an impressive, world-class team of Volume Editors and Contributing Authors. Each chapter has been painstakingly reviewed and checked for consistent high quality. The result is an authoritative overview which ties the literature together and provides the user with a reliable background information and citation resource |
| methods in molecular biophysics: Biophysical Techniques Iain Campbell, 2012-02-16 Biophysical Techniques explains in a readily-accessible way the basics of the various biophysical methods available so students can understand the principles behind the different methods used, and begin to appreciate which tools can be used to probe different biological questions, and the pros and cons of each. |
| methods in molecular biophysics: Handbook of Single-Molecule Biophysics Peter Hinterdorfer, Antoine van Oijen, 2009-12-24 This handbook describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping. It includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells. |
| methods in molecular biophysics: Methods of Molecular Analysis in the Life Sciences Andreas Hofmann, Anne Simon, Tanja Grkovic, Malcolm Jones, 2014-06-19 An accessible overview of the most popular and cutting-edge methods for studying the properties of molecules and their interactions. |
| methods in molecular biophysics: Biophysics of Membrane Proteins Vincent L. G. Postis, Adrian Goldman, 2021-02-14 This volume provides recent advances in the field of biophysics of membrane proteins. Chapters are divided into several parts: detailing biochemistry and functional analysis, experimental and theoretical structural determinations, membrane protein dynamics, and conformation studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Biophysics of Membrane Proteins: Methods and Protocols aims to provide comprehensive protocols with notes to help further the understanding of key membrane protein structure and function for students, academics, and industrial researchers. |
| methods in molecular biophysics: Fundamentals of Polymer Physics and Molecular Biophysics Himadri B. Bohidar, 2015-01-05 Provides a physical interpretation of the data obtained in macromolecular transport phenomena in a given system and also addresses some important issues and concepts related to biopolymers such as proteins and nucleic acids-- |
| methods in molecular biophysics: Computer Simulation and Data Analysis in Molecular Biology and Biophysics Victor Bloomfield, 2009-06-05 This book provides an introduction to two important aspects of modern bioch- istry, molecular biology, and biophysics: computer simulation and data analysis. My aim is to introduce the tools that will enable students to learn and use some f- damental methods to construct quantitative models of biological mechanisms, both deterministicandwithsomeelementsofrandomness;tolearnhowconceptsofpr- ability can help to understand important features of DNA sequences; and to apply a useful set of statistical methods to analysis of experimental data. The availability of very capable but inexpensive personal computers and software makes it possible to do such work at a much higher level, but in a much easier way, than ever before. TheExecutiveSummaryofthein?uential2003reportfromtheNationalAcademy of Sciences, “BIO 2010: Transforming Undergraduate Education for Future - search Biologists” [12], begins The interplay of the recombinant DNA, instrumentation, and digital revolutions has p- foundly transformed biological research. The con?uence of these three innovations has led to important discoveries, such as the mapping of the human genome. How biologists design, perform, and analyze experiments is changing swiftly. Biological concepts and models are becoming more quantitative, and biological research has become critically dependent on concepts and methods drawn from other scienti?c disciplines. The connections between the biological sciences and the physical sciences, mathematics, and computer science are rapidly becoming deeper and more extensive. |
| methods in molecular biophysics: Biophysics for Beginners Helmut Schiessel, 2013-12-20 Biophysics is a new way of looking at living matter. It uses quantitative experimental and theoretical methods to open a new window for studying and understanding life processes. This textbook gives compact introductions to the basics of the field, including molecular cell biology and statistical physics. It then presents in-depth discussions of more advanced biophysics subjects, progressing to state-of-the-art experiments and their theoretical interpretations. The book is unique by offering a general introduction to biophysics, yet at the same time restricting itself to processes that occur inside the cell nucleus and that involve biopolymers (DNA, RNA, and proteins). This allows for an accessible read for beginners and a springboard for specialists who wish to continue their study in more detail. |
| methods in molecular biophysics: Biophysics W. Hoppe, W. Lohmann, H. Markl, H. Ziegler, 2012-12-06 What is biophysics? As with all subjects which straddle traditional boundaries between fields, it eludes a precise definition. Furthermore, it is impossible to do biophysics without having a certain foundation of knowledge in biology, physics, physical chemistry, chemistry and biochemistry. One approach to a biophysics textbook would be to refer the student to the literature of these neighboring fields, and to leave the selection of the appropriate supplementary material up to the student. The editors of this volume are of the opinion that it is more useful and less time-consuming to present a selection of the supplementary knowledge, in concentrated form, together with the subject matter specific to biophysics. The reader will thus find in this book introductions to such subjects as the structure and function of the cell, the chemical structure of biogenic macromolecules, and even theoretical chemistry. What, indeed, is biophysics? Must we consider it to include physiology, electromedicine, radiation medicine, etc. ? The field has evolved continuously in recent years. Molecular understanding oflife processes has come more and more to the fore. Just as the field of molecular physics has developed to describe structures and processes in the realm of non-living systems, there has been a corresponding development of molecular biophysics. |
| methods in molecular biophysics: Advanced Techniques in Biophysics José Luis R. Arrondo, Alicia Alonso, 2007-04-21 Technical advancements are basic elements in our life. In biophysical studies, new applications and improvements in well-established techniques are being implemented every day. This book deals with advancements produced not only from a technical point of view, but also from new approaches that are being taken in the study of biophysical samples, such as nanotechniques or single-cell measurements. This book constitutes a privileged observatory for reviewing novel applications of biophysical techniques that can help the reader enter an area where the technology is progressing quickly and where a comprehensive explanation is not always to be found. |
| methods in molecular biophysics: Applied Biophysics Thomas Andrew Waigh, 2007-09-11 This book presents the fundamentals of molecular biophysics, and highlights the connection between molecules and biological phenomena, making it an important text across a variety of science disciplines. The topics covered in the book include: Phase transitions that occur in biosystems (protein crystallisation, globule-coil transition etc) Liquid crystallinity as an example of the delicate range of partially ordered phases found with biological molecules How molecules move and propel themselves at the cellular level The general features of self-assembly with examples from proteins The phase behaviour of DNA The physical toolbox presented within this text will form a basis for students to enter into a wide range of pure and applied bioengineering fields in medical, food and pharmaceutical areas. |
| methods in molecular biophysics: Advances in Protein Molecular and Structural Biology Methods Timir Tripathi, Vikash Kumar Dubey, 2022-01-14 Advances in Protein Molecular and Structural Biology Methods offers a complete overview of the latest tools and methods applicable to the study of proteins at the molecular and structural level. The book begins with sections exploring tools to optimize recombinant protein expression and biophysical techniques such as fluorescence spectroscopy, NMR, mass spectrometry, cryo-electron microscopy, and X-ray crystallography. It then moves towards computational approaches, considering structural bioinformatics, molecular dynamics simulations, and deep machine learning technologies. The book also covers methods applied to intrinsically disordered proteins (IDPs)followed by chapters on protein interaction networks, protein function, and protein design and engineering. It provides researchers with an extensive toolkit of methods and techniques to draw from when conducting their own experimental work, taking them from foundational concepts to practical application. - Presents a thorough overview of the latest and emerging methods and technologies for protein study - Explores biophysical techniques, including nuclear magnetic resonance, X-ray crystallography, and cryo-electron microscopy - Includes computational and machine learning methods - Features a section dedicated to tools and techniques specific to studying intrinsically disordered proteins |
| methods in molecular biophysics: Methods in Molecular Biophysics , 2015 |
| methods in molecular biophysics: Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology Keith Wilson, Andreas Hofmann, John M. Walker, Samuel Clokie, 2018-04-19 A major update of a best-selling textbook that introduces students to the key experimental and analytical techniques underpinning life science research. |
| methods in molecular biophysics: Molecular and Cellular Biophysics Meyer B. Jackson, 2006-01-12 Molecular and Cellular Biophysics provides advanced undergraduate and graduate students with a foundation in the basic concepts of biophysics. Students who have taken physical chemistry and calculus courses will find this book an accessible and valuable aid in learning how these concepts can be used in biological research. The text provides a rigorous treatment of the fundamental theories in biophysics and illustrates their application with examples. Conformational transitions of proteins are studied first using thermodynamics, and subsequently with kinetics. Allosteric theory is developed as the synthesis of conformational transitions and association reactions. Basic ideas of thermodynamics and kinetics are applied to topics such as protein folding, enzyme catalysis and ion channel permeation. These concepts are then used as the building blocks in a treatment of membrane excitability. Through these examples, students will gain an understanding of the general importance and broad applicability of biophysical principles to biological problems. |
| methods in molecular biophysics: Computational Biochemistry and Biophysics Oren M. Becker, Alexander D. MacKerell Jr., Benoit Roux, Masakatsu Watanabe, 2001-02-09 Covering theoretical methods and computational techniques in biomolecular research, this book focuses on approaches for the treatment of macromolecules, including proteins, nucleic acids, and bilayer membranes. It uses concepts in free energy calculations, conformational analysis, reaction rates, and transition pathways to calculate and interpret b |
| methods in molecular biophysics: Biophysics William Bialek, 2012-10-28 A physicist's guide to the phenomena of life Interactions between the fields of physics and biology reach back over a century, and some of the most significant developments in biology—from the discovery of DNA's structure to imaging of the human brain—have involved collaboration across this disciplinary boundary. For a new generation of physicists, the phenomena of life pose exciting challenges to physics itself, and biophysics has emerged as an important subfield of this discipline. Here, William Bialek provides the first graduate-level introduction to biophysics aimed at physics students. Bialek begins by exploring how photon counting in vision offers important lessons about the opportunities for quantitative, physics-style experiments on diverse biological phenomena. He draws from these lessons three general physical principles—the importance of noise, the need to understand the extraordinary performance of living systems without appealing to finely tuned parameters, and the critical role of the representation and flow of information in the business of life. Bialek then applies these principles to a broad range of phenomena, including the control of gene expression, perception and memory, protein folding, the mechanics of the inner ear, the dynamics of biochemical reactions, and pattern formation in developing embryos. Featuring numerous problems and exercises throughout, Biophysics emphasizes the unifying power of abstract physical principles to motivate new and novel experiments on biological systems. Covers a range of biological phenomena from the physicist's perspective Features 200 problems Draws on statistical mechanics, quantum mechanics, and related mathematical concepts Includes an annotated bibliography and detailed appendixes |
| methods in molecular biophysics: Biochemistry, Biophysics, and Molecular Chemistry Francisco Torrens, Debarshi Kar Mahapatra, A. K. Haghi, 2020-04-07 Biochemistry, Biophysics, and Molecular Chemistry: Applied Research and Interactions provides the background needed in biophysics and molecular chemistry and offers a great deal of advanced biophysical knowledge. It emphasizes the growing interrelatedness of molecular chemistry and biochemistry, and acquaints one with experimental methods of both disciplines. This book addresses some of the enormous advances in biochemistry, particularly in the areas of structural biology and bioinformatics, by providing a solid biochemical foundation that is rooted in chemistry. Topics include scientific integrity and ethics in the field; clinical translational research in cancer, diabetes, and cardiovascular disease; emerging drugs to treat neurodegenerative diseases; swine, avian, and human flu; the use of big data in artificial knowledge in the field; bioinformatic insights on molecular chemistry; and much more. |
| methods in molecular biophysics: Computational Biophysics of Membrane Proteins Carmen Domene, 2016-11-30 Exploring current themes in modern computational and membrane protein biophysics, this book presents a comprehensive account of the fundamental principles underlying different methods and techniques used to describe the intriguing mechanisms by which membrane proteins function. The book discusses the experimental approaches employed to study these proteins, with chapters reviewing recent crucial structural advances that have allowed computational biophysicists to discern how these molecular machines work. The book then explores what computational methods are available to researchers and what these have taught us about three key families of membrane proteins: ion channels, transporters and receptors. The book is ideal for researchers in computational chemistry and computational biophysics. |
| methods in molecular biophysics: Biophysical Techniques in Drug Discovery Angeles Canales, 2017-11-14 Biophysical techniques are used in many key stages of the drug discovery process including in screening for new receptor ligands, in characterising drug mechanisms, and in validating data from biochemical and cellular assays. This book provides an overview of the biophysical methods applied in drug discovery today, including traditional techniques and newer developments. Perspectives from academia and industry across a spectrum of techniques are brought together in a single volume. Small and biotherapeutic approaches are covered and strengths and limitations of each technique are presented. Case studies illustrate the application of each technique in real applied examples. Finally, the book covers recent developments in areas such as electron microscopy with discussions of their possible impact on future drug discovery. This is a go-to volume for biophysicists, analytical chemists and medicinal chemists providing a broad overview of techniques of contemporary interest in drug discovery. |
| methods in molecular biophysics: The Physics of Proteins Hans Frauenfelder, 2010-05-30 Provides an introduction to the structure and function of biomolecules --- especially proteins --- and the physical tools used to investigate them The discussion concentrates on physical tools and properties, emphasizing techniques that are contributing to new developments and avoiding those that are already well established and whose results have already been exploited fully New tools appear regularly - synchrotron radiation, proton radiology, holography, optical tweezers, and muon radiography, for example, have all been used to open new areas of understanding |
| methods in molecular biophysics: Protein Physics Alexei V. Finkelstein, Oleg Ptitsyn, 2016-06-22 Protein Physics: A Course of Lectures covers the most general problems of protein structure, folding and function. It describes key experimental facts and introduces concepts and theories, dealing with fibrous, membrane, and water-soluble globular proteins, in both their native and denatured states. The book systematically summarizes and presents the results of several decades of worldwide fundamental research on protein physics, structure, and folding, describing many physical models that help readers make estimates and predictions of physical processes that occur in proteins. New to this revised edition is the inclusion of novel information on amyloid aggregation, natively disordered proteins, protein folding in vivo, protein motors, misfolding, chameleon proteins, advances in protein engineering & design, and advances in the modeling of protein folding. Further, the book provides problems with solutions, many new and updated references, and physical and mathematical appendices. In addition, new figures (including stereo drawings, with a special appendix showing how to use them) are added, making this an ideal resource for graduate and advanced undergraduate students and researchers in academia in the fields of biophysics, physics, biochemistry, biologists, biotechnology, and chemistry. - Fully revised and expanded new edition based on the latest research developments in protein physics - Written by the world's top expert in the field - Deals with fibrous, membrane, and water-soluble globular proteins, in both their native and denatured states - Summarizes, in a systematic form, the results of several decades of worldwide fundamental research on protein physics and their structure and folding - Examines experimental data on protein structure in the post-genome era |
| methods in molecular biophysics: Molecular Biophysics M Volkenstein, 1977 Molecular Biophysics presents the fundamental principles of biophysics and their application to the study of the physical properties of biological macromolecules. The merger of biology and physics involves the development of sophisticated instrumentation and the molecular approach to the study of life phenomena. This book is composed of nine chapters and begins with an overview of the thermodynamical aspects and chemical foundations of biophysics. These topics are followed by the physical aspects of macromolecules, with a particular emphasis on the biological functions, conformation, and hydrophobic interactions of proteins. The subsequent chapter describes the structural and electro-optical properties of biopolymers based on X-ray, optical, and spectroscopic analysis. The discussion then shifts to enzymes, their chemical kinetics, catalytic potential, and conformational and cooperative properties. The remaining chapters explore the physical aspects of nucleic acids and the biosynthesis of proteins. This book will prove useful to molecular biophysicists, biologists, physicists, and researchers in the fields of life sciences. |
| methods in molecular biophysics: Essentials of Biophysics P. Narayanan, 2010 Biophysics is the bridge between the physical and natural sciences. There is a direct correlation and equivalence between physical and medical/biological science. The aim of this book is to present all the topics that can be classified as essential to biophysics: - chemical bonding (quantum physics), cellular, molecular and membrane biophysics, biophysical chemistry of biomolecules, physiochemical techniques (spectroscopies, microscopies, NMR, X-Ray diffraction), bioenergetics, biomechanics, photo-chemo- and radiation biophysics, and bioinformatics. The contents are organised into 4 sections containing 18 sub-sections. An overview is given for each section that comprises several chapters with a common theme. Each chapter is summarised in advance with a handy synopsis to assist the reader's understanding. Further reading is suggested at the end of every chapter. And the appendices and glossary at the end of the book provide an easy access to the more technical concepts, phrases and terminology. Essentials of Biophysics can therefore by definition be used on a wide range of cross disciplinary science courses, and will also serve as a valuable reference work in physical and natural science libraries. |
| methods in molecular biophysics: Methods in Plant Molecular Biology Mary A. Schuler, Raymond E. Zielinski, 2012-12-02 Methods in Plant Molecular Biology is a lab manual that introduces students to a diversity of molecular techniques needed for experiments with plant cells. Those included have been perfected and are now presented for the first time in a usable and teachable form. Because the manual integrates protein, RNA, and DNA techniques, it will serve students, teachers, and researchers in plant physiology, biophysics, and animal molecular biology who have no previous experience handling recombinant DNA or purified proteins. It can also be used by the established molecular biologist who wishes to utilize the powerful techniques of recombinant DNA to explore the mysteries of the plant kingdom. - Eight basic experiments which can be used collectively or individually cover - Recombinant Cloning and Screening in E. coli; DNA Sequencing - Plant RNA Isolation and in Vitro Translations - Plant DNA Isolations and Genomic DNA Southern AnalysisChloroplast Isolation and Protein SynthesisPlant Tissue Culture and Agrobacterium Transformations - Experiments that have been student tested for three years - Blueprints for setting up gel rigs - Comprehensive course schedule outlining individual procedures to be finished in each lab segment - Course can be tailored to suit the needs of the individual instructor |
| methods in molecular biophysics: Molecular Biology Nancy Craig, Rachel Green, Orna Cohen-Fix, Carol Greider, Gisela Storz, Cynthia Wolberger, 2014-05 The biological world operates on a multitude of scales - from molecules to tissues to organisms to ecosystems. Throughout these myriad levels runs a common thread: the communication and onward passage of information, from cell to cell, from organism to organism and ultimately, from generation to generation. But how does this information come alive to govern the processes that constitute life? The answer lies in the molecular components that cooperate through a series of carefully-regulated processes to bring the information in our genome to life. These components and processes lie at the heart of one of the most fascinating subjects to engage the minds of scientists today: molecular biology. Molecular Biology: Principles of Genome Function, Second Edition, offers a fresh approach to the teaching of molecular biology by focusing on the commonalities that exist between the three kingdoms of life, and discussing the differences between the three kingdoms to offer instructive insights into molecular processes and components. This gives students an accurate depiction of our current understanding of the conserved nature of molecular biology, and the differences that underpin biological diversity. Additionally, an integrated approach demonstrates how certain molecular phenomena have diverse impacts on genome function by presenting them as themes that recur throughout the book, rather than as artificially separated topics As an experimental science, molecular biology requires an appreciation for the approaches taken to yield the information from which concepts and principles are deduced. Experimental Approach panels throughout the text describe research that has been particularly valuable in elucidating difference aspects of molecular biology. Each panel is carefully cross-referenced to the discussion of key molecular biology tools and techniques, which are presented in a dedicated chapter at the end of the book. Molecular Biology further enriches the learning experience with full-color artwork, end-of-chapter questions and summaries, suggested further readings grouped by topic, and an extensive glossary of key terms. Features: A focus on the underlying principles of molecular biology equips students with a robust conceptual framework on which to build their knowledge An emphasis on their commonalities reflects the processes and components that exist between bacteria, archae, and eukaryotes Experimental Approach panels demonstrate the importance of experimental evidence by describing research that has been particularly valuable in the field |
| methods in molecular biophysics: Chemical Biophysics Daniel A. Beard, Hong Qian, 2010-11-25 Chemical Biophysics provides an engineering-based approach to biochemical system analysis for graduate-level courses on systems biology, computational bioengineering and molecular biophysics. It is the first textbook to apply rigorous physical chemistry principles to mathematical and computational modeling of biochemical systems for an interdisciplinary audience. The book is structured to show the student the basic biophysical concepts before applying this theory to computational modeling and analysis, building up to advanced topics and research. Topics explored include the kinetics of nonequilibrium open biological systems, enzyme mediated reactions, metabolic networks, biological transport processes, large-scale biochemical networks and stochastic processes in biochemical systems. End-of-chapter exercises range from confidence-building calculations to computational simulation projects. |
| methods in molecular biophysics: Introduction to Experimental Biophysics - A Laboratory Guide Jay L. Nadeau, 2015-03-25 Easily Get Started with Biological ExperimentsIntroduction to Experimental Biophysics - A Laboratory Guide presents wet lab methods for courses in biophysics or molecular biology. A companion to the author's highly praised An Introduction to Experimental Biophysics: Biological Methods for Physical Scientists, this manual offers a flexible course pl |
| methods in molecular biophysics: Biophysics of RNA-Protein Interactions Chirlmin Joo, David Rueda, 2019-09-19 RNA molecules play key roles in all aspects of cellular life, but to do so efficiently, they must work in synergism with proteins. This book addresses how proteins and RNA interact to carry out biological functions such as protein synthesis, regulation of gene expression, genome defense, liquid phase separation and more. The topics addressed in this volume will appeal to researchers in biophysics, biochemistry and structural biology. The book is a useful resource for anybody interested in elucidating the molecular mechanisms and discrete properties of RNA-protein complexes. Included are reviews of key systems such as microRNA and CRISPR/Cas that exemplify how RNA and proteins work together to perform their biological function. Also covered are techniques ranging from single molecule fluorescence and force spectroscopy to crystallography, cryo-EM microscopy, and kinetic modeling. |
Methods - University of Nebraska Medical Center
Methods Mol. Biol. 416:103-116. Fey PD, Endres JL, Yajjala VK, Widhelm TJ, Boissy RJ, Bose JL, and Bayles KW. 2013. A genetic resource for rapid and comprehensive phenotype …
Tn Insertion Map
Please note that because the NTML website has been updated to the S. aureus JE2 genome, the transposon insertion site location numbers now reflect the JE2 genome numbering.
Tools - University of Nebraska Medical Center
Methods Tools Contact The NTML Screening Array The Nebraska Transposon Mutant Library (NTML) Screening Array for phenotype screens consists of 1,920 transposon (Tn) mutants of …
Contact - ntml.unmc.edu
Methods Tools Contact Center for Staphylococcal Research (CSR) Department of Pathology & Microbiology University of Nebraska Medical Center 985900 Nebraska Medical Center …
Stats - University of Nebraska Medical Center
Staphylococcus aureus subsp. aureus USA300_FPR3757 GenBank RefSeq features: Feature type Count Count % Length (bp, total) Length % [Genome] 2,872,769
EcoRI (2) TN up eqFP650 TIR oriV TN down
pFP650-R 8773 bp Ampr Cmr eqFP650 Counter selection TN up TN down TIR pE194ts oriV EcoRI (2) SalI(1793) E. coli Features -oriV: high copy origin -Ampr: Ampicillin resistance (100 …
EcoRI (2) TN up TIR r eqFP650 oriV TN down - University of …
pFP650-F 8773 bp Ampr Cmr eqFP650 Counter selection TN up TN down TIR pE194ts oriV EcoRI (2) SalI(1793) E. coli Features -oriV: high copy origin -Ampr: Ampicillin resistance (100 …
Methods - University of Nebraska Medical Center
Methods Mol. Biol. 416:103-116. Fey PD, Endres JL, Yajjala VK, Widhelm TJ, Boissy RJ, Bose JL, and Bayles KW. 2013. A genetic resource for rapid and comprehensive phenotype …
Tn Insertion Map
Please note that because the NTML website has been updated to the S. aureus JE2 genome, the transposon insertion site location numbers now reflect the JE2 genome numbering.
Tools - University of Nebraska Medical Center
Methods Tools Contact The NTML Screening Array The Nebraska Transposon Mutant Library (NTML) Screening Array for phenotype screens consists of 1,920 transposon (Tn) mutants of …
Contact - ntml.unmc.edu
Methods Tools Contact Center for Staphylococcal Research (CSR) Department of Pathology & Microbiology University of Nebraska Medical Center 985900 Nebraska Medical Center …
Stats - University of Nebraska Medical Center
Staphylococcus aureus subsp. aureus USA300_FPR3757 GenBank RefSeq features: Feature type Count Count % Length (bp, total) Length % [Genome] 2,872,769
EcoRI (2) TN up eqFP650 TIR oriV TN down
pFP650-R 8773 bp Ampr Cmr eqFP650 Counter selection TN up TN down TIR pE194ts oriV EcoRI (2) SalI(1793) E. coli Features -oriV: high copy origin -Ampr: Ampicillin resistance (100 …
EcoRI (2) TN up TIR r eqFP650 oriV TN down - University of …
pFP650-F 8773 bp Ampr Cmr eqFP650 Counter selection TN up TN down TIR pE194ts oriV EcoRI (2) SalI(1793) E. coli Features -oriV: high copy origin -Ampr: Ampicillin resistance (100 …
