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| fanzor is a eukaryotic programmable rna-guided endonuclease: Shaping sustainable agrifood futures: pre-emerging and emerging technologies and innovations for impact Alexandrova-Stefanova, N.; Nosarzewski, K.; Mroczek, Z.K.; Audouin, S.; Djamen, P.; Kolos, N.; Wan, J., 2024-10-16 FAO’s Office of Innovation is working with CIRAD (International Cooperation Centre of Agricultural Research for Development) and other partners on an FAO initiative on foresight on pre-emerging and emerging agrifood technologies and innovations, aligned with UN 2.0 and The Future of Food and Agriculture 2022: engaging all key actors of agrifood innovation systems in the foresight on pre-emerging and emerging technologies and innovations (PETIAS) to better prepare for alternative futures, feeding it into anticipatory action, and convening the global community for constructive multilateral dialogue and knowledge exchange. The aim is to support policymakers, investors and innovation actors in their approaches and decision making.The study assesses a selection of technologies and innovations, which potentially could be of paramount importance in addressing agrifood challenges until 2050, as well as the most important trends and drivers that will influence the emergence of agrifood PETIAS and their triggers of change, including some regional aspects and stakeholders' perspectives. The goal is also to build plausible future scenarios for the evolvement of the PETIAS and innovation process governance in the future with the time horizon of 2050 to inform future-oriented policymaking.The report is built with inputs from a multistakeholder Delphi survey, online and in presence workshops with experts and stakeholders, the FAO FSN Forum, as well as regional foresight exercises (Latin America and Central Asia and Caucasus). |
| fanzor is a eukaryotic programmable rna-guided endonuclease: Transcriptional Regulation by Neuronal Activity Ramendra N. Saha, Serena M. Dudek, 2024-11-12 This book discusses the regulation of gene transcription by neuronal activity that is evident in a large number of neuronal processes ranging from neural development and refinement of neuronal connections to learning and response to injury. Transcriptional Regulation by Neuronal Activity: To the Nucleus and Back, 2nd edition illustrates how signals are transmitted to the nucleus in response to neuronal activity, which genes are regulated and how this is achieved, and how these changes in gene expression alter neuronal function. The aim of this second edition is to highlight key advances in the field since the first edition. The book is divided into four sections. The first highlights how signals get to the nucleus from the membrane in response to synaptic or neuronal activity. Included are chapters on the pathways that transmit signals from synapses to nuclei. The second section focuses on epigenetic regulatory processes of activity-induced gene transcription, an area that has exploded in the past few years. The third section navigates the role of activity-induced genes in physiological processes such as learning and memory, and human developmental disorders such as those associated with the autism spectrum. The fourth section highlights groundbreaking technological advances in the field, which have allowed activity-regulated transcription to be used as a tool to study learning and memory. |
| fanzor is a eukaryotic programmable rna-guided endonuclease: Introduction to CRISPR-Cas9 Techniques Michael J. Wolyniak, Donna L. Pattison, Jay N. Pieczynski, Maria S. Santisteban, 2025-02-17 This open-access textbook provides an in-depth introduction into the CRISPR-cas9 technology and explores its use across the gamut of biological model systems. As the subject has risen from a significant new discovery to a mainstream molecular biology practice, it is essential that students of molecular biology understand the fundamentals behind CRISPR-Cas9 technology and how it may be employed efficiently and ethically in research. This volume, edited by experts in both, molecular biology and undergraduate education, will teach not only the fundamentals of using CRISPR-Cas9, but also how to successfully employ this technology in classroom settings. The book is written for undergraduates and advanced high school classes in the area of molecular biology, genetics, genomics and biological engineering and will provide a perfect tool for undergraduate lecturers to prepare their classes. |
| fanzor is a eukaryotic programmable rna-guided endonuclease: Epigenetics in Biological Communication Guenther Witzany, 2024-06-19 Every cell, tissue, organ and organism is competent to use signs to exchange information reaching common coordinations and organisations of both single cell and group behavior. These sign-mediated interactions we term biological communication. The regulatory system that works in development, morphology, cell fate and identity, physiology, genetic instructions, immunity, memory/learning, physical and mental disease depends on epigenetic marks. The communication of cells, persistent viruses and their defectives such as mobile genetic elements and RNA networks ensures both the transport of regulatory instructions and the reprogramming of these instructions. But how are the different states of the epigenome orchestrated? The epigenetic pathways respond to various signaling cues such as DNA methylation, histone variants, histone modifications, chromatin structure, nucleosome remodeling, and epigenetic interactions. Epigenetic signals are responsible for the establishment, maintenance and reversal of transcriptional states that are fundamental for the cell's ability to memorize past events, such as changes in the external environment, socio-sphere or developmental cues. External signals trigger changes in the epigenome, allowing cells to respond dynamically. Internal signals direct activities that are necessary for body maintenance, and repairing damaged tissues and organs. With the emergence of epigenetic memory, organisms can fix historical and context dependent impressive experiences. Evolution from now on learnt to learn. Learning means organisms can avoid reproduction of always the same. This is key to adaptation. However, inheritance of acquired characteristics is only one of the many examples of the explanatory power of epigenetics. Behavioral epigenetics demonstrates the way in which environmental and social experiences produce individual differences in behaviour, cognition, personality, and mental health. This book assembles experts to outline the various motifs of all kinds of epigenetic regulation of cells throughout their lives. |
| fanzor is a eukaryotic programmable rna-guided endonuclease: CRISPR-Cas Systems for RNA and Genome Editing , 2025-03-25 CRISPR-Cas Systems for RNA and Genome Editing, Volume 711 in this ongoing series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as Massively parallel genomic perturbations with multi-target CRISPR interrogates Cas9 activity and DNA repair at endogenous sites, Genome oligopaint via local denaturation fluorescence in situ hybridization, Biochemical reconstitution of a type I-B CRISPR-associated transposon, Visualizing the conformational landscape of CRISPR-Cas9 through kinetics-informed structural studies, and much more.Additional sections cover Analysis of metal-dependent DNA nicking activities by Cas endonucleases, Biochemically characterizing the accessory proteins and ribonucleoprotein complexes of Type IV CRISPR systems, Prime editing in bacteria with BacPE, Preparation of high-purity CRISPR-based DNA base editor RNPs, Bacterial directed evolution of CRISPR base editors, Cloning and validating systems for high throughput molecular recording, Using CRISPR-Cas13 for viral nucleic acid detection, and more. - Provides the latest information on DNA/RNA research - Offers outstanding and original reviews on a range of DNA/RNA research topics - Serves as an indispensable reference for researchers and students alike |
| fanzor is a eukaryotic programmable rna-guided endonuclease: Flipons Alan Herbert, 2024-03-27 Alan Herbert has played a leading part in discovering the biological roles for a high-energy form of DNA twisted to the left rather than to the right. Both Z-DNA and the Z-RNA sensing proteins are critical for protecting hosts against both viruses and cancers. The proteins also play critical roles in the programmed cell death of aging cells. Other types of flipons exist and alter the readout of transcripts from the genome, encoding genetic information by their shape rather than by their sequence. Many of these flipons are within repeat elements that were previously considered to be just genomic junk. Instead these genetic elements increase the adaptability of cells by flipping DNA conformation. By acting as digital switches, the different flipon types can alter cellular responses without any change to their sequence or any damage to DNA. These highly dynamic structures enable the rapid evolution of multicellular organisms. The junk DNA in repeats also encode peptide patches that enable the assembly of cellular machines. The intransitive logic involved enhances the chance of an individual surviving a constantly changing environment. Key Features Causes us to rethink how information is encoded in the genome Changes our understanding of how our genome evolved and how we protect ourselves against viruses and cancers while sparing normal cells Shows that high energy forms of DNA, such as left-handed DNA do exist inside the cell Accessible to those in academia and the general public, and speaks to the next generation, encouraging them to find their own path in scientific discovery The Open Access version of this book, available at http://www.taylorfrancis.com, has been made available under a Creative Commons Attribution-Non-Commercial-No Derivative License (CC-BY-NC-ND) 4.0 license. |
| fanzor is a eukaryotic programmable rna-guided endonuclease: Art and Biotechnology Claire Correo Nettleton, Louise Mackenzie, 2024-06-13 This interdisciplinary anthology examines the relationship between developments in biotechnology and both artistic and literary innovation, focussing in particular on how newfound molecular technologies and knowledge regimes, such as CRISPR gene editing, alter conceptions of what it means to be human. The book presents 21 essays, split across four parts, from a coterie of artists, theorists, historians and scientists which examine the symbiotic relationship between humans, animals, and viruses as well as the impossibility of germ-free existence. The essays in this volume are urgent in their topicality, embodying the exhilarating yet alarming zeitgeist of contemporary nonhuman-to-human viral transmission and gene editing technologies. Ultimately, Art and Biotechnology reveals how art and biotechnology influence each other and how art has shaped the discussion around gene editing and the socio-cultural aspects of the Covid-19 pandemic. It is essential reading for students and researchers focussing on science and art, environmental humanities, and ethics. |
| fanzor is a eukaryotic programmable rna-guided endonuclease: Frontiers in global regulatory landscape of CRISPR-edited plants Aftab Ahmad, Neil E. Hoffman, Michael George Kepler Jones, Baohong Zhang, 2024-02-07 |
| fanzor is a eukaryotic programmable rna-guided endonuclease: CRISPR for Climate-Smart and Sustainable Agriculture Jen-Tsung Chen, 2025-03-28 CRISPR for Climate-Smart and Sustainable Agriculture summarizes the current genome editing technology being used in food and energy crops. It presents methods and applications of CRISPR tools for breeding climate-smart and stress-tolerant crops that are better able to resist biotic and abiotic stressors due to climate change.Gene editing tools are increasingly seen as options for improving plant health and productivity. CRISPR is one such emerging and quickly advancing crucial technology for the research field of plant physiology, plant molecular biology, and crop breeding. CRISPR for Climate-Smart and Sustainable Agriculture focuses on genetic engineering using CRISPR technology for precision breeding of crops to achieve climate resilience, stress tolerance, and higher yield. Organized by specific plant challenges, this book presents expert insights from around the globe, providing updated knowledge of CRISPR with an emphasis on facing climate change and supporting sustainable agriculture.This volume in the Genome Modified Plants and Microbes in Food and Agriculture series will be valuable for researchers, academics, and advanced-level students seeking to understand and advance the use of CRISPR technologies toward the UN Sustainable Development Goals and the Paris Climate Agreement. - Focuses on the precision breeding of crops to achieve stress resilience, high quality, and high yield - Includes genome editing technologies to support climate-smart and sustainable agriculture - Summarizes the latest findings of plant-CRISPR approaches |
| fanzor is a eukaryotic programmable rna-guided endonuclease: Innovations in Development, Translational Research and Manufacturing of CAR T cells Michael Hudecek, Qasim Rafiq, Stephen Goldrick, Ulrike Koehl, Helene Negre, Carmen Sanges, 2024-10-03 Immunotherapy with genetically engineered immune cell products is a transformative treatment modality with potential applications in various fields of medicine. A prime example is chimeric antigen receptor (CAR)-modified T cells in hematology and oncology, and the advent of CAR T cell therapies to treat infectious diseases, autoimmune disorders, and cardiovascular diseases. The medical need and demand from patients and caregivers require radical innovations to accelerate and improve pre-clinical development and clinical translation, provision of gene-transfer vectors, and immune cell product manufacturing as well as a critical reflection and discussion on ethical and socioeconomic aspects. The goal of this special issue of Frontiers in Immunology is to provide a comprehensive and multi-faceted view on the current state-of-the-art, imminent and future directions the field is taking in order to accelerate the pre-clinical development, clinical translation, and manufacturing of CAR T cells, increase access and sustainability of CAR T cell therapy for health care systems (in developed and in developing countries). This special issue will focus on the medical and scientific dimension incl. approved and emerging indications, new areas in medicine, advanced gene-transfer and gene-editing technologies, innovations in pre-clinical assessment (efficacy, toxicology, genomic safety), innovations in scalable automated manufacturing (bioprocessing), the implementation of high content data acquisition, machine learning and artificial intelligence, innovations in clinical trial design; and consider the ethical, socioeconomic and societal dimension of CAR T cells in particular and gene-engineered immune cell therapy in general. |
| fanzor is a eukaryotic programmable rna-guided endonuclease: Gene-Edited Crops Aftab Ahmad, Nayla Munawar, Baohong Zhang, 2025-06-30 CRISPR-based genome editing has revolutionized the precise genetic improvement of agriculturally important crops, offering unprecedented precision and efficiency in crop improvement. This powerful technology holds immense potential to reshape the future of food security, enabling scientists and agriculturalists to develop resilient, high yield crops resistant to diseases, pests, and environmental stresses. Gene-Edited Crops: The CRISPR Solution for Global Food Security offers a comprehensive guide to the latest research, advancements, and real-world applications of CRISPR in crop genetic improvement for sustainable agriculture and food security. Through in-depth chapters, this book provides an accessible yet rigorous exploration of CRISPR technology, CRISPR reagents and their potential delivery methods, recent developments in CRISPR, and its transformative role in agriculture and food security. With a focus on practical implementation and future potential, this book examines the science behind genome editing and the strategic pathways to address key challenges in crop development—from enhancing nutritional profiles to improving environmental adaptability. Features include: Comprehensive overview: An introduction to the fundamentals of CRISPR technology and its application in crop science. Computational tools and CRISPR reagents: Bioinformatic tools for designing precise gRNA, CRISPR reagents and their effective delivery methods. Recent developments in CRISPR: Emerging developments in CRISPR for precise gene editing in plants such as base editing, prime editing, Fanzor, and multiplex gene editing. Real-world applications: Application of CRISPR for developing future crops, including projects targeting enhanced disease resistance, drought tolerance, and improved nutritional content. Regulatory landscape: Insightful discussion on the evolving global regulatory framework governing gene-edited crops, including the transformative role of artificial intelligence in navigating these regulations. Future directions: Exploration of ongoing advances and the outlook for CRISPR technology in addressing food security. With detailed examples, data, and resources, this book serves as an essential reference for scientists, agriculturalists, and policymakers invested in the future of food security. It also doubles as an educational resource for advanced courses in genetics, plant sciences, and agricultural biotechnology, making it invaluable to both seasoned researchers and those newly interested in the potential of CRISPR. |
| fanzor is a eukaryotic programmable rna-guided endonuclease: CRISPR-Cas Systems Rodolphe Barrangou, John van der Oost, 2012-12-13 CRISPR/Cas is a recently described defense system that protects bacteria and archaea against invasion by mobile genetic elements such as viruses and plasmids. A wide spectrum of distinct CRISPR/Cas systems has been identified in at least half of the available prokaryotic genomes. On-going structural and functional analyses have resulted in a far greater insight into the functions and possible applications of these systems, although many secrets remain to be discovered. In this book, experts summarize the state of the art in this exciting field. |
| fanzor is a eukaryotic programmable rna-guided endonuclease: Mobile DNA III Michael Chandler, Martin Gellert, Alan M. Lambowitz, Phoebe A. Rice, Suzanne B. Sandmeyer, 2020-07-24 An exploration of the raw power of genetic material to refashion itself to any purpose... Virtually all organisms contain multiple mobile DNAs that can move from place to place, and in some organisms, mobile DNA elements make up a significant portion of the genome. Mobile DNA III provides a comprehensive review of recent research, including findings suggesting the important role that mobile elements play in genome evolution and stability. Editor-in-Chief Nancy L. Craig assembled a team of multidisciplinary experts to develop this cutting-edge resource that covers the specific molecular mechanisms involved in recombination, including a detailed structural analysis of the enzymes responsible presents a detailed account of the many different recombination systems that can rearrange genomes examines the tremendous impact of mobile DNA in virtually all organisms Mobile DNA III is valuable as an in-depth supplemental reading for upper level life sciences students and as a reference for investigators exploring new biological systems. Biomedical researchers will find documentation of recent advances in understanding immune-antigen conflict between host and pathogen. It introduces biotechnicians to amazing tools for in vivo control of designer DNAs. It allows specialists to pick and choose advanced reviews of specific elements and to be drawn in by unexpected parallels and contrasts among the elements in diverse organisms. Mobile DNA III provides the most lucid reviews of these complex topics available anywhere. |
| fanzor is a eukaryotic programmable rna-guided endonuclease: CRISPR-Cas Enzymes , 2019-01-25 CRISPR-Cas Enzymes, Volume 616, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Topics covered in this release include CRISPR bioinformatics, A method for one-step assembly of Class 2 CRISPR arrays, Biochemical reconstitution and structural analysis of ribonucleoprotein complexes in Type I-E CRISPR-Cas systems, Mechanistic dissection of the CRISPR interference pathway in Type I-E CRISPR-Cas system, Site-specific fluorescent labeling of individual proteins within CRISPR complexes, Fluorescence-based methods for measuring target interference by CRISPR-Cas systems, Native State Structural Characterization of CRISRP Associated Complexes using Mass Spectrometry, and more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Enzymology series - Updated release includes the latest information on the CRISPR-Cas Enzymes |
| fanzor is a eukaryotic programmable rna-guided endonuclease: Prokaryotic Toxin-Antitoxins Kenn Gerdes, 2012-10-19 Prokaryotic Toxins – Antitoxins gives the first overview of an exciting and rapidly expanding research field. Toxin – antitoxin (TA) genes were discovered on plasmids 30 years ago. Since then it has become evident that TA genes are highly abundant in bacterial and archaeal chromosomes. TA genes code for an antitoxin that combine with and neutralize a cognate toxin. When activated, the toxins inhibit protein synthesis and cell growth and thereby induce dormancy and multidrug tolerance (persistence). Remarkably, in some species, the TA gene families have undergone dramatic expansions. For example, the highly persistent major human pathogen Mycobacterium tuberculosis has »100 TA loci. The large expansion of TA genes by some organisms is a biological mystery. However, recent observations indicate that TA genes contribute cumulatively to the persistence of bacteria. This medically important phenomenon may thus for the first time become experimentally tractable at the molecular level. |
| fanzor is a eukaryotic programmable rna-guided endonuclease: CRISPR Rodolphe Barrangou, Erik J. Sontheimer, Luciano A. Marraffini, 2022-09-19 “An excellent compendium of all things CRISPR from some of the leading minds in the field. With thorough coverage from every angle and beautifully detailed illustrations, this book is not to be missed!” Jennifer A. Doudna, Professor of Chemistry, Biochemistry & Molecular Biology, UC Berkeley; Founder, Innovative Genomics Institute; Nobel Laureate and coinventor of CRISPR technology “This journey through CRISPR biology and several of its breakthrough applications offers an exciting glimpse into one of the most beautiful and compelling fields in the life sciences.” David R. Liu, Director of the Merkin Institute at the Broad Institute of MIT and Harvard; Professor of Chemistry and Chemical Biology at Harvard University; coinventor of base editing and prime editing “A must read! The CRISPR topics, written by world-leading experts, span from the fascinating mechanistic underpinnings to the ingenious applications. One can read from start to finish or pick and choose themes. Either way, the book delivers utterly enjoyable learning!” Bonnie Bassler, Squibb Professor and Chair, Princeton University Department of Molecular Biology; Howard Hughes Medical Institute Investigator CRISPR-Cas systems have revolutionized the science of gene editing and their possible applications continue to expand, from basic research to potentially groundbreaking medical and commercial uses. Led by a distinguished team of editors, CRISPR: Biology and Applications explores the subject matter needed to delve into this fascinating area. Topics covered include: Classification and molecular mechanisms of CRISPR-Cas systems CRISPR-Cas evolution, regulation, expression, and function Uses for gene editing and modulation of gene expression CRISPR-based antimicrobials and phage resistance for medical and industrial purposes Written by internationally renowned authors, CRISPR: Biology and Applications serves as both an introductory guide for those new to the field and an authoritative reference for seasoned researchers whose work touches this evolving and headline- making science. |
| fanzor is a eukaryotic programmable rna-guided endonuclease: Regulating with RNA in Bacteria and Archaea Gisela Storz, Kai Papenfort, 2018-11-01 Revealing the many roles of RNA in regulating gene expression For decades after the discoveries of messenger RNA, transfer RNA, and ribosomal RNA, it was largely assumed that the role of RNA in the cell was limited to shuttling the genomic message, chaperoning amino acids, and toiling in the ribosomes. Eventually, hints that RNA molecules might have regulatory roles began to appear. With the advent of genomics and bioinformatics, it became evident that numerous other RNA forms exist and have specific functions, including small RNAs (sRNA), RNA thermometers, and riboswitches to regulate core metabolic pathways, bacterial pathogenesis, iron homeostasis, quorum sensing, and biofilm formation. All of these functions, and more, are presented in Regulating with RNA in Bacteria and Archaea, written by RNA biologists from around the globe. Divided into eight sections-RNases and Helicases, Cis-Acting RNAs, Cis Encoded Base Pairing RNAs, Trans-Encoded Base Pairing RNAs, Protein Titration and Scaffolding, General Considerations, Emerging Topics, and Resources-this book serves as an excellent resource for established RNA biologists and for the many scientists who are studying regulated cellular systems. It is no longer a fair assumption that gene expression regulation is the provenance of proteins only or that control is exerted primarily at the level of transcription. This book makes clear that regulatory RNAs are key partners along with proteins in controlling the complex interactions and pathways found within prokaryotes. |
| fanzor is a eukaryotic programmable rna-guided endonuclease: Regulation of Bacterial Virulence Michael L. Vasil, Andrew J. Darwin, 2012-12-05 A comprehensive compendium of scholarly contributions relating to bacterial virulence gene regulation. • Provides insights into global control and the switch between distinct infectious states (e.g., acute vs. chronic). • Considers key issues about the mechanisms of gene regulation relating to: surface factors, exported toxins and export mechanisms. • Reflects on how the regulation of intracellular lifestyles and the response to stress can ultimately have an impact on the outcome of an infection. • Highlights and examines some emerging regulatory mechanisms of special significance. • Serves as an ideal compendium of valuable topics for students, researchers and faculty with interests in how the mechanisms of gene regulation ultimately affect the outcome of an array of bacterial infectious diseases. |
| fanzor is a eukaryotic programmable rna-guided endonuclease: Regulatory RNAs in Prokaryotes Anita Marchfelder, Wolfgang Hess, 2012-12-23 This book provides a comprehensive and up-to-date collection of review articles focusing on RNA-mediated regulation in prokaryotes. The various modes of action include the direct interaction with proteins, direct sensing of metabolites or of physical parameters, and the interaction with RNAs to stimulate or prevent binding of ribosomes or to stimulate degradation. Written by leading experts in the field, the book covers small RNA functions, RNA thermometers, riboswitches, the diversity of small RNA-guided CRISPR-Cas defense systems and selected RNA chaperons in both prokaryotic domains, bacteria and archaea. Recent advances towards the computational identification of regulatory RNAs and their targets are included and particular attention is paid to small RNA in pathogenic bacteria. This volume is the only one exclusively covering regulatory RNAs in the prokaryotic domains to date, making it essential literature for anyone interested in RNA function and gene regulation and a valuable resource for teaching these concepts. |
| fanzor is a eukaryotic programmable rna-guided endonuclease: Handbook of RNA Biochemistry Roland K. Hartmann, Albrecht Bindereif, Astrid Sch¿n, Eric Westhof, 2015-06-22 The second edition of a highly acclaimed handbook and ready reference. Unmatched in its breadth and quality, around 100 specialists from all over the world share their up-to-date expertise and experiences, including hundreds of protocols, complete with explanations, and hitherto unpublished troubleshooting hints. They cover all modern techniques for the handling, analysis and modification of RNAs and their complexes with proteins. Throughout, they bear the practising bench scientist in mind, providing quick and reliable access to a plethora of solutions for practical questions of RNA research, ranging from simple to highly complex. This broad scope allows the treatment of specialized methods side by side with basic biochemical techniques, making the book a real treasure trove for every researcher experimenting with RNA. |
| fanzor is a eukaryotic programmable rna-guided endonuclease: Artificial Riboswitches Atsushi Ogawa, 2016-09-03 Artificial riboswitches and other ligand-responsive gene regulators make it possible to switch protein synthesis ON or OFF with arbitrary ligand molecules. Artificial Riboswitches: Methods and Protocols focuses on the state-of-the-art methods developed in recent years for creating artificial riboswitches, therefore this volume could be regarded as a collection of recipes for the gene circuit elements in synthetic biology and metabolic engineering. Chapters cover topics such as screening or rational design methods for obtaining artificial riboswitches that function in either bacterial or eukaryotic translational systems, protocols for evaluating the activities of the resultant riboswitches, as well as protocols for construction of ligand-dependent, trans-acting gene regulators. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Artificial Riboswitches: Methods and Protocols seeks to serve not only bioengineers who aim to reprogram cell behaviors and molecular biologists who leverage these regulators for genetic studies, but to all researchers interested in this fascinating field. |
| fanzor is a eukaryotic programmable rna-guided endonuclease: RNA Sequence, Structure, and Function: Computational and Bioinformatic Methods Jan Gorodkin, Walter L. Ruzzo, 2014-03-18 The existence of genes for RNA molecules not coding for proteins (ncRNAs) has been recognized since the 1950's, but until recently, aside from the critically important ribosomal and transfer RNA genes, most focus has been on protein coding genes. However, a long series of striking discoveries, from RNA's ability to carry out catalytic function, to discovery of riboswitches, microRNAs and other ribo-regulators performing critical tasks in essentially all living organisms, has created a burgeoning interest in this primordial component of the biosphere. However, the structural characteristics and evolutionary constraints on RNA molecules are very different from those of proteins, necessitating development of a completely new suite of informatic tools to address these challenges. In RNA Sequence, Structure, Function: Computational and Bioinformatic Methods, expert researchers in the field describe a substantial and relevant fraction of these methodologies from both practical and computational/algorithmic perspectives. Focusing on both of these directions addresses both the biologist interested in knowing more about RNA bioinformatics as well as the bioinformaticist interested in more detailed aspects of the algorithms. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results. Thorough and intuitive, RNA Sequence, Structure, Function: Computational and Bioinformatic Methods aids scientists in continuing to study key methods and principles of RNA bioinformatics. |
| fanzor is a eukaryotic programmable rna-guided endonuclease: CRISPR Magnus Lundgren, Emmanuelle Charpentier, Peter C. Fineran, 2016-10-09 This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels. CRISPR: Methods and Protocols guides readers through techniques that have been developed specifically for the analysis of CRISPR-Cas and techniques adapted from standard protocols of DNA, RNA and protein biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, CRISPR: Methods and Protocols provides a broad list of tools and techniques to study the interdisciplinary aspects of the prokaryotic CRISPR-Cas defense systems. |
| fanzor is a eukaryotic programmable rna-guided endonuclease: High-Density Sequencing Applications in Microbial Molecular Genetics , 2018-12-06 High-Density Sequencing Applications in Microbial Molecular Genetics, Volume 612 in the Methods of Enzymology series provides the latest on the high-density sequencing of DNA and cDNA libraries and how they have revolutionized contemporary research in biology. Methods permitting tens of millions of sequence reads in a single experiment have paved the way to genome-wide studies that are contributing to our understanding of the complexity of living systems. Chapters in this updated volume include Characterizing the role of exoribonucleases in the control of microbial gene expression: Differential RNA seq., Conformational studies of bacterial chromosomes by high-throughput sequencing methods, Measuring mRNA degradation, and more. Addition sections cover Global recognition patterns of bacterial RNA-binding proteins, High-resolution profiling of NMD targets, and the Generation of a metagenomic 3C/Hi-C library of human gut microbiota, Genome-wide mapping of yeast retrotransposons integration target sites, Measuring protein synthesis rates, Finding unsuspected partners of small RNAs with new screening approaches, Use of multiplexed transcriptomics to define the relationship between promoter sequence and transcription output, RNA-based control of quorum sensing in Vibrio cholerae, amongst other highly regarded topics. |
| fanzor is a eukaryotic programmable rna-guided endonuclease: Riboswitches Alexander Serganov, 2009-04-17 The transfer of hereditary information from genes to proteins is one of the essential pr- esses in all living organisms on our planet. Some genes are expressed without modu- tion throughout the life of a cell, while many others require various degrees of control to precisely balance cellular metabolism with environmental conditions. For many years, researchers attributed this regulatory function to protein molecules, which can direct gene expression at multiple levels, in response to various input signals, and with different degrees of selectivity. Even when the control of gene expression was achieved via direct interactions between proteins and mRNAs, the active role was routinely assigned to p- teins, while RNAs were considered merely as recipient molecules. The discovery of RNA interference and multiple bacterial regulatory RNAs caused a shift from the perception of proteins as the predominant regulators of gene expression to the acknowledgement of the importance of RNAs in many regulatory circuits. Such a viewpoint received strong support several years ago after the discovery of riboswitches and related RNA sensors – mRNA regions capable of alternating their conformations in response to the presence of cellular metabolites and other physical or chemical cues. These classes of RNA pass on cellular and environmental information directly to transcription or translation machinery without the assistance of proteins. The riboswitches are commonly defined as evolutionarily conserved mRNA regions capable of specific binding to metabolite molecules, and, as a result, adopting a particular RNA conformation that modulates gene expression. |
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GitHub - MNprojects/DokkanAPI
This API is designed to allow the user to query for character data for the mobile game Dragon Ball Z Dokkan Battle. Data is restricted to only LR and UR characters. This API is in open beta, so …
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An API actually made for dokkan correctly. Huge project but I plan to make it right. - Bouncer011/DokkanBattleAPI
Useful Dokkan sites for teambuilding, links etc : r ... - Reddit
Nov 29, 2021 · trueNew users? I've been playing for like 4.5 years and I find these extremely useful for team building 😂 I suppose it comes down to time consumption, I'd rather use these …
Looking for a Dokkan Battle database or API : r/DBZDokkanBattle
Sep 8, 2020 · Everything about Dragon Ball Z: Dokkan Battle! This subreddit is for both the Global and Japanese versions of the game. Please feel free to share information, guides, tips, news, …
r/DBZDokkanBattle on Reddit: Build teams, check linking partners …
Mar 9, 2023 · Build teams, check linking partners and more, DokkanTools.com on your phone is here! Big update, asking for feedback to make it better!
