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| elisa theory and practice: ELISA John R. Crowther, 2008-02-02 ELISA: Theory and Practice introduces to scientists at all levels of expertise the principles of the most commonly used assay technique known as the Enzyme Linked Immunosorbent Assay. The book provides readers with full descriptions of the basic systems that make ELISA one of the most powerful techniques in science today, and also examines in detail the data obtained by ELISA and their analysis and actual manipulation. ELISA: Theory and Practice is designed not only to train novices in the science of ELISA, but also to aid investigators experienced in any of the biological sciences in performing independently assays of antibodies and antigens. Mastery of the book's contents will allow readers to fully appreciate exactly how and why assays function, as well as permit the efficient development of individual assays that are both rapid and accurate. |
| elisa theory and practice: Elisa: Theory and Practice. Methods in Molecular Biology, Volume 42 John R. Crowther, 1995 ELISA: Theory and Practice introduces to scientists at all levels of expertise the principles of the most commonly used assay technique known as the Enzyme Linked Immunosorbent Assay. The book provides readers with full descriptions of the basic systems that make ELISA one of the most powerful techniques in science today, and also examines in detail the data obtained by ELISA and their analysis and actual manipulation. ELISA: Theory and Practice is designed not only to train novices in the science of ELISA, but also to aid investigators experienced in any of the biological sciences in performing independently assays of antibodies and antigens. Mastery of the book's contents will allow readers to fully appreciate exactly how and why assays function, as well as permit the efficient development of individual assays that are both rapid and accurate. |
| elisa theory and practice: Elisa: Theory And Practice Crowther, 2007-12-01 |
| elisa theory and practice: ELISA , 1995 ELISA: Theory and Practice introduces to scientists at all levels of expertise the principles of the most commonly used assay technique known as the Enzyme Linked Immunosorbent Assay. The book provides readers with full descriptions of the basic systems that make ELISA one of the most powerful techniques in science today, and also examines in detail the data obtained by ELISA and their analysis and actual manipulation. ELISA: Theory and Practice is designed not only to train novices in the science of ELISA, but also to aid investigators experienced in any of the biological sciences in performing independently assays of antibodies and antigens. Mastery of the book's contents will allow readers to fully appreciate exactly how and why assays function, as well as permit the efficient development of individual assays that are both rapid and accurate. |
| elisa theory and practice: Elisa Patricia Marques, 2017-11 ELISA (enzyme-linked immunosorbent assay) revolutionized the way to detect antibodies and antigens, by using an enzymatic system instead of radioactive labelling. This techniques has been widely use since its development for disease diagnostics and other purposes. A quick Pubmed search will give you more than 23000 manuscripts peer-reviewed and published, using this assay. The ELISA technique was conceptualized and developed by Peter Perlmann, and Eva Engvall at Stockholm University, Sweden. Engvall and Perlmann published their first paper on ELISA in 1971. Solid-phase techniques were used in the development of microtiter plates (96 wells) in which either an antigen or an antibody is non-covalently bound to a solid-phase support. Technical advances led to automated pipetting devices (Micromedics; Hamilton), multichannel pipettes (Lab Systems), and microtiter plate readers and washers, and in the 1980s fully automated test instruments were manufactured by Boehringer-Mannheim and Abbott, among others. Such automated systems have come to stay in medical laboratories.This book starts by introducing the ELISA history and the three different main forms of the assay, Direct, Indirect, and Sandwich ELISA. It continues with a general overview of different ELISA methods using LPS, peptides as antigens and urine the sample for diagnosis (Section 1). The next chapters focus on different forms of the ELISA assays, peptide ELISA (Section 2), microarrays, microfluidics CD-ELISA and live-cell ELISA (Section 3). The next Sections are dedicated to the application of ELISA to the diagnosis of Malaria (Sections 4), other infectious diseases (Human papillomavirus, Dengue, Prions, Cystic Fibrosis, Leptospirosis, Herpes simplex related to HIV and Hepatitis C related to HIV (Section 5), different types of Cancer (Section 6) and Alzheimer's disease looking at biomarkers in sera and Cerebrospinal fluid (Section 7). This book is only a short overview of the big field that is the ELISA technique world. With new methods being developed daily. New methods combining this method with RFLP, PCR, microarray, microfluidics, live-cell imaging, and so on.New Infectious diseases, neurologic diseases, cancer and auto immune diseases diagnostic methods based on ELISA techniques are being commercialized and made available daily. |
| elisa theory and practice: Methods in Molecular Biology: ELISA : theory and practice John M. Walker, 1984 |
| elisa theory and practice: The Immunoassay Handbook David Wild, 2013-01-21 The fourth edition of The Immunoassay Handbook provides an excellent, thoroughly updated guide to the science, technology and applications of ELISA and other immunoassays, including a wealth of practical advice. It encompasses a wide range of methods and gives an insight into the latest developments and applications in clinical and veterinary practice and in pharmaceutical and life science research. Highly illustrated and clearly written, this award-winning reference work provides an excellent guide to this fast-growing field. Revised and extensively updated, with over 30% new material and 77 chapters, it reveals the underlying common principles and simplifies an abundance of innovation. The Immunoassay Handbook reviews a wide range of topics, now including lateral flow, microsphere multiplex assays, immunohistochemistry, practical ELISA development, assay interferences, pharmaceutical applications, qualitative immunoassays, antibody detection and lab-on-a-chip. This handbook is a must-read for all who use immunoassay as a tool, including clinicians, clinical and veterinary chemists, biochemists, food technologists, environmental scientists, and students and researchers in medicine, immunology and proteomics. It is an essential reference for the immunoassay industry. Provides an excellent revised guide to this commercially highly successful technology in diagnostics and research, from consumer home pregnancy kits to AIDS testing.www.immunoassayhandbook.com is a great resource that we put a lot of effort into. The content is designed to encourage purchases of single chapters or the entire book. David Wild is a healthcare industry veteran, with experience in biotechnology, pharmaceuticals, medical devices and immunodiagnostics, which remains his passion. He worked for Amersham, Eastman-Kodak, Johnson & Johnson, and Bristol-Myers Squibb, and consulted for diagnostics and biotechnology companies. He led research and development programs, design and construction of chemical and biotechnology plants, and integration of acquired companies. Director-level positions included Research and Development, Design Engineering, Operations and Strategy, for billion dollar businesses. He retired from full-time work in 2012 to focus on his role as Editor of The Immunoassay Handbook, and advises on product development, manufacturing and marketing. - Provides a unique mix of theory, practical advice and applications, with numerous examples - Offers explanations of technologies under development and practical insider tips that are sometimes omitted from scientific papers - Includes a comprehensive troubleshooting guide, useful for solving problems and improving assay performancee - Provides valuable chapter updates, now available on www.immunoassayhandbook.com |
| elisa theory and practice: The ELISA Guidebook J. R. Crowther, 2009-01-01 This text presents the fundamental aspects of the heterogeneous enzyme linked immunosorbent assay (ELISA). Designed for the newcomer as well as the experienced practitioner, it gives numerous examples of the use of ELISA. |
| elisa theory and practice: Molecular Biomethods Handbook Ralph Rapley, John M. Walker, 2007-10-09 An authoritative team of investigators illuminate the core bioanalytical techniques used every day in their own laboratories, and laboratories throughout the world. These highly experienced scientists fully explain both the theory behind, and the application of, these key techniques, and include extensive references for those seeking detailed laboratory protocols. The techniques covered range from the extraction, separation, detection, and characterization of nucleic acids to gene cloning and library production, mapping, expression, transgenesis, differential display, and DNA profiling, to name a few. Numerous key protein methods, as well as support and related techniques, are also included. The goal is to provide established scientists and novices who are new to these techniques with a deeper understanding of the widest variety of biotechniques and their applications. |
| elisa theory and practice: Antibody Engineering Protocols Sudhir Paul, 2008-02-02 This comprehensive collection of recently developed methods for producing new antibody reagents by immunization and recombinant DNA techniques contains ready-to-use protocols that illuminate current areas of research on antibody structure, functions, and applications. The methods can be applied in basic immunological studies involving antibody specificity, catalysis, and evolution, and in the isolation of rare antibodies by phage display technology and the engineering of new antibodies by mutagenesis. They offer insight into new ways of developing clinically useful antibody reagents. Antibody Engineering Protocols constitutes a single-source volume for laboratory investigators who want to minimize extensive literature and methodology searches and to work productively in their fields with reproducible step-by-step protocols. |
| elisa theory and practice: Basic Cell Culture Protocols Jeffrey W. Pollard, John M. Walker, 1997 Now completely revised and updated from the original, much-acclaimed and bestselling first edition, Basic Cell Culture Protocols, 2nd ed. offers today's most comprehensive collection of easy-to-follow, cutting-edge protocols for the culture of a wide range of animal cells. Its authoritative contributors provide explicit, step-by-step instructions, along with extensive notes and tips that allow both experts and beginners to successfully achieve their desired results. Topics range from basic culture methodology to strategies for culturing previously uncultured cell types and hard-to-culture differentiated cells. Methods are also provided for the analysis of living cells by FACS, video microscopy, and confocal microscopy. Like the first edition, this book should be in every cell culture laboratory and be of use to all who use cell cultures in research. |
| elisa theory and practice: Fundamentals of BioMEMS and Medical Microdevices Steven Saliterman, 2006 The world is on the threshold of a revolution that will change medicine and how patients are treated forever. Bringing together the creative talents of electrical, mechanical, optical and chemical engineers, materials specialists, clinical-laboratory scientists, and physicians, the science of biomedical microelectromechanical systems (bioMEMS) promises to deliver sensitive, selective, fast, low cost, less invasive, and more robust methods for diagnostics, individualized treatment, and novel drug delivery. This book is an introduction to this multidisciplinary technology and the current state of micromedical devices in use today. The first text of its kind dedicated to bioMEMS training. Fundamentals of BioMEMS and Medical Microdevices is Suitable for a single semester course for senior and graduate-level students, or as an introduction to others interested or already working in the field. |
| elisa theory and practice: Gene Isolation and Mapping Protocols Jacqueline Boultwood, 2008-02-02 An unprecedented collection of all the most up-to-date techniques for gene isolation and mapping, including the latest methods for gene characterization using database analyses. This collection of thoroughly tested recipes also includes chapters for the computational analysis of novel cDNA sequences with up-to-the-minute information on basic sequence analysis, sequence similarity searches, exon detection and similarity searches, and the prediction of gene function. Its state-of-the-art methods constitute indispensable tools for all scientists engaged in the search for specific disease genes, or in the general advancement of the human genome project. |
| elisa theory and practice: Labs on Chip Eugenio Iannone, 2018-09-03 Labs on Chip: Principles, Design and Technology provides a complete reference for the complex field of labs on chip in biotechnology. Merging three main areas— fluid dynamics, monolithic micro- and nanotechnology, and out-of-equilibrium biochemistry—this text integrates coverage of technology issues with strong theoretical explanations of design techniques. Analyzing each subject from basic principles to relevant applications, this book: Describes the biochemical elements required to work on labs on chip Discusses fabrication, microfluidic, and electronic and optical detection techniques Addresses planar technologies, polymer microfabrication, and process scalability to huge volumes Presents a global view of current lab-on-chip research and development Devotes an entire chapter to labs on chip for genetics Summarizing in one source the different technical competencies required, Labs on Chip: Principles, Design and Technology offers valuable guidance for the lab-on-chip design decision-making process, while exploring essential elements of labs on chip useful both to the professional who wants to approach a new field and to the specialist who wants to gain a broader perspective. |
| elisa theory and practice: Protein Purification Protocols Shawn Doonan, 2008-02-02 Hans Neurath has written that this is the second golden era of enzymology {Protein Science [1994], vol. 3, pp. 1734—1739); he could with justice have been more general and referred to the second golden age of protein chemistry. The last two decades have seen enormous advances in our understanding of the structures and functions of pro teins arising on the one hand from improvements and developments in analytical techniques {see the companion volume, Basic Protein and Peptide Protocols, in this series) and on the other hand from the tech nologies of molecular genetics. Far from turning the focus away from protein science, the ability to isolate, analyze, and express genes has increased interest in proteins as gene products. Hence, many laborato ries are now getting involved in protein isolation for the first time, either as an essential adjunct to their work in molecular genetics or because of a curiosity to know more about the products of the genes that they have been studying. Protein Purification Protocols is aimed mainly at these newcom ers to protein purification, but it is hoped that it will also be of value to established practitioners who may find here techniques that they have not tried, but which might well be most applicable in their work. With the exception mainly of the first and last chapters, the format of the contributions to the present book conform to the established format of the Methods in Molecular Biology series. |
| elisa theory and practice: Glycobiology Protocols Inka Brockhausen, 2008-02-04 Glycobiology involves studies of complex carbohydrates and posttrans- tional modifications of proteins, and has become an important interdiscip- nary field encompassing chemistry, biochemistry, biology, physiology, and pathology. Although initial research was directed toward elucidation of the different carbohydrate structures and the enzymes synthesizing them, the field has now moved toward identifying the functions of carbohydrates. The pro- cols described in Glycobiology Protocols form a solid basis for investigations of glycan functions in health and disease. The cloning of many of the genes participating in glycosylation processes has helped to enhance our knowledge of how glycosylation is controlled, but has also added another dimension of complexity to the great heterogeneous variety of the structures of the oligos- charides of glycoproteins, proteoglycans, and glycolipids. A family of similar enzyme proteins exists for each glycosylation step. Glycosyltransferases are extremely specific for both the nucleotide sugar donor and the acceptor s- strate, but many other factors control sugar transfer, including the locali- tion and topology of enzymes, cofactors, possible chaperone proteins, and the availability of sugar acceptor substrates. The analysis of the intracellular organization of glycosylation and of the factors controlling the activities of the participating enzymes in the cell are important areas that need more research efforts. Another challenge for future research is to understand the glycodynamics of a cell, that is, how the cell responds to stimuli leading to biological and pathological changes in terms of alterations in glycosylation, and how this affects the biology of the cell. |
| elisa theory and practice: Activity Theory in Practice Harry Daniels, Anne Edwards, Yrjo Engeström, Tony Gallagher, Sten R. Ludvigsen, 2013-05-13 This ground-breaking book brings together cutting-edge researchers who study the transformation of practice through the enhancement and transformation of expertise. This is an important moment for such a contribution because expertise is in transition - moving toward collaboration in inter-organizational fields and continuous shaping of transformations. To understand and master this transition, powerful new conceptual tools are needed and are provided here. The theoretical framework which has shaped these studies is Cultural Historical Activity Theory (CHAT). CHAT analyses how people and organisations learn to do something new, and how both individuals and organisations change. The theoretical and methodological tools used have their origins in the work of Lev Vygotsky and A.N. Leont’ev. In recent years this body of work has aroused significant interest across the social sciences, management and communication studies. Working as part of an integrated international team, the authors identify specific findings which are of direct interest to the academic community, such as: the analysis of vertical learning between operational and strategic levels within complex organizations; the refinement of notions of identity and subject position within CHAT; the introduction of the concept of ‘labour power’ into CHAT; the development of a method of analysing discourse which theoretically coheres with CHAT and the design of projects. Activity Theory in Practice will be highly useful to practitioners, researchers, students and policy-makers who are interested in conceptual and empirical issues in all aspects of ‘activity-based’ research. |
| elisa theory and practice: Capillary Electrophoresis Guidebook Kevin D. Altria, 2008-02-02 This book is intended to be a working guide to the operation of capillary electrophoresis (CE) instrumentation. Since CE is still a rap idly maturing technique, detailed validated protocols are not widely established. Therefore, extensive experimental procedures are not pro vided for individual analyses. The intention is to provide general guide lines on the principles and practice of CE and to give an overview of the specific technologies and important application areas. Part I provides operating instructions for standard commercially available instruments. Guidelines are included for activities such as changing capillaries, method development, quantitative procedures, optimization of precision and sensitivity, and the validation of meth ods, fraction collection, and troubleshooting, as well as a quick guide to running a separation. The application range of CE is possibly the most diverse of all analytical techniques and ranges from large, complex macromolecules, such as proteins and nucleic acids, to small solutes, such as organic drugs and inorganic anions and cations. |
| elisa theory and practice: The Liberal Project and Human Rights John Charvet, Elisa Kaczynska-Nay, 2010-06-04 The 'Liberal Project' aims to transform society in accordance with liberal values and practices. This volume argues that the United Nations regime on human rights is an attempt to realise this project on an international level. The authors provide an engaging theoretical and historical context for this argument, defining the concept of liberalism, its origins and evolution, and identify it as a universal value that constitutes the very essence of the international human rights regime. The book explores the possibility of a cross-cultural consensus on the issue being reached, but problems of sovereignty and nationalism are also discussed as potential obstacles to the Liberal Project's completion. This penetrating and insightful work will appeal to a wide range of scholars and students interested in liberalism and human rights from the fields of international relations, law, political theory and political philosophy. |
| elisa theory and practice: Rapid Immunotests for Clinical, Food and Environmental Applications , 2016-07-01 Irina Goryacheva authors this volume titled Rapid Immunotests for Clinical, Food and Environmental Applications that is devoted to the latest research in the area of the construction and application of rapid immunotests with plasmonic and luminescent detection, with special attention paid to the achievements of nanotechnology in the areas of labels and solid supports creation.With close attention to the basic principles and the specific issues, considering the breadth of the field that the rapid tests may offer, the coverage of this book is by no means complete, keeping open space for challenge and research - Provides comprehensive coverage of rapid immunotests for clinical, food, and environmental applications - Explores a variety of specialized techniques - Presents a general overview of imaging techniques in diverse fields |
| elisa theory and practice: Smartphones for Chemical Analysis: From Proof-of-concept to Analytical Applications , 2023-04-21 Smartphones for Chemical Analysis: From Proof-of-Concept to Analytical Applications, Volume 101 in the Comprehensive Analytical Chemistry series, highlights new advances in this broadening field, with chaters that cover Smartphone-based assay benchmarking using traditional instrumental analysis, Smartphone-based water analysis, Sample preparation in smartphone-based analysis: Current status and challenges, Application of smartphone-based analysis in the medical field, Smartphone-based biosensors in the food analysis field, Bioreceptors for smartphone detection, Smartphone anatomy: Features used for ubiquitous sensing, and much more. Additional chapters cover End-user integration for at-home analysis, Hyphenating paper-based biosensors with smartphones, Smartphone based Fluorescent and chemiluminescent sensing, Smartphones for portable surface enhanced raman spectroscopy, Towards a universal applicable photonics approach and sustainable spectral data, Integrating blockchain and image analyses on smartphones to create a secure food export pipeline, Smartphone-based electrochemical (bio)sensors: state of the art and perspective, Micro-and nanoplastic detection through a point of site platform platform, and Benchmarking using ambient mass spectrometry. - Provides up-to-date, expert opinion on this emerging topic - Covers a wider range of the chemical analysis field, from food to medical analysis - Highlights current challenges with solutions proposed |
| elisa theory and practice: Development and Application of Biomarkers Roger L. Lundblad, 2016-04-19 First introduced to biomedical research in 1980, the term biomarker has taken on a life of its own in recent years and has come to mean a number of things. A comprehensive assessment of biomarkers, this book covers the history and current status of the application of biomarkers in diagnostics and prognostics. It explores the technology used for the study of biomarkers, and the validation of biomarkers including a comparison of the various technologies used to identify and measure biomarkers. The editors emphasize the technology underlying biomarkers and the translation of basic science to clinical laboratory technology, including the commercial development of biomarkers. The book also covers proteomics and proteomic technologies and their applications in the identification of biomarkers. |
| elisa theory and practice: Protein Targeting Protocols Roger A. Clegg, 2008-02-04 It is by no means a revelation that proteins are not uniformly distributed throughout the cell. As a result, the idea that protein molecules, because of the specificity with which they can engage in interactions with other proteins, may be aimed—via these interactions—at a restricted target, is a fundamental one in contemporary molecular life sciences. The target may be variously c- ceived as a specific molecule, a group of molecules, a structure, or a more generic type of intracellular environment. Because the concept of protein targeting is intuitive rather than expl- itly defined, it has been variously used by different groups of researchers in cell biology, biochemistry, and molecular biology. For those working in the field of intracellular signaling, an influential introduction to the topic was the seminal article by Hubbard & Cohen (TIBS [1993] 18, 172–177), which was based on the work of Cohen’s laboratory on protein phosphatases. Sub- quently, the ideas that they discussed have been further developed and extended by many workers to other key intermediaries in intracellular sign- ing, including protein kinases and a great variety of modulator and adaptor proteins. |
| elisa theory and practice: Rethinking College Student Development Theory Using Critical Frameworks Elisa S. Abes, Susan R. Jones, D-L Stewart, 2023-07-03 A major new contribution to college student development theory, this book brings third wave theories to bear on this vitally important topic. The first section includes a chapter that provides an overview of the evolution of student development theories as well as chapters describing the critical and poststructural theories most relevant to the next iteration of student development theory. These theories include critical race theory, queer theory, feminist theories, intersectionality, decolonizing/indigenous theories, and crip theories. These chapters also include a discussion of how each theory is relevant to the central questions of student development theory. The second section provides critical interpretations of the primary constructs associated with student development theory. These constructs and their related ideas include resilience, dissonance, socially constructed identities, authenticity, agency, context, development (consistency/coherence/stability), and knowledge (sources of truth and belief systems). Each chapter begins with brief personal narratives on a particular construct; the chapter authors then re-envision the narrative’s highlighted construct using one or more critical theories. The third section will focus on implications for practice. Specifically, these chapters will consider possibilities for how student development constructs re-envisioned through critical perspectives can be utilized in practice. The primary audience for the book is faculty members who teach in graduate programs in higher education and student affairs and their students. The book will also be useful to practitioners seeking guidance in working effectively with students across the convergence of multiple aspects of identity and development. |
| elisa theory and practice: Bioconjugation Protocols Christof M. Niemeyer, 2008-02-04 There are a number of outstanding volumes that provide a comprehensive overview of bioconjugation techniques. However, many of the conventional approaches to the synthesis of chemically modified protein conjugates lack efficient means to control the stoichiometry of conjugation, as well as the s- cific site of attachment of the conjugated moiety. Moreover, the recent dev- opments in microarray technologies as well as in nanobiotechnology—a novel field of research rapidly evolving at the crossroads of physics, chemistry, b- technology, and materials science—call for a summary of modern bioconjugation strategies to overcome the limitations of the classical approaches. Bioconjugation Protocols: Methods and Strategies is intended to provide an update of many of the classic techniques and also to introduce and summarize newer approaches that go beyond the pure biomedical applications of bioconjugation. The purpose of Bioconjugation Protocols: Methods and Str- egies is therefore to provide instruction and inspiration for all those scientists confronting the challenges of semisynthesizing functional biomolecular reagents for a wide variety of applications ranging from novel biomedical diagnostics, to therapeutics, to biomaterials. Part I contains seven protocols for the preparation of protein conjugates. |
| elisa theory and practice: Interleukin Protocols Luke A. J. O’Neill, Andrew Bowie, 2008-02-02 Interleukins are a family of proteins that regulate the maturation, diff- entiation, or activation of cells involved in immunity and inflammation, and belong to a broader family termed cytokines. Collectively these proteins are the key orchestrators of host defense and the response to tissue injury. There are currently 23 different interleukins (numbered from IL-1 to IL-23), although the full extent of the interleukin family will only become clear upon analysis of the human genome sequence. Most important, interleukins are central to the pathogenesis of a wide range of diseases that involve an immune com- nent, including such conditions as rheumatoid arthritis, multiple sclerosis, ulcerative colitis, psoriasis, and asthma. Interleukins have also been imp- cated in other conditions, including cancer, migraine, myocardial infarction, and depression. In essence, when cells are activated by interleukins, a program of gene expression is initiated in the target cell that alters the cell’s phenotype, leading to enhanced immune reactivity, inflammation, and/or proliferation. Interleukins are therefore at the core of the cellular basis for many diseases. They are the subject of intense investigation by biomedical researchers and the targeting or use of interleukins in the clinic is proceeding apace. Approaches such as t- geting IL-4 in asthma or IL-1 in joint disease are being pursued, and it is likely that in the next 5–10 years a number of new therapies based on either inhib- ing or administering interleukins will be available. |
| elisa theory and practice: Animal Cell Electroporation and Electrofusion Protocols Jac A. Nickoloff, 2008-02-03 The ability to introduce macromolecules into animal cells, includ ing DNA, RNA, proteins, and other bioactive compounds has facili tated a broad range of biological studies, from biochemistry and biophysics to molecular biology, cell biology, and whole animal stud ies. Gene transfer technology in particular will continue to play an essential role in studies aimed at improving our understanding of the relationships between the gene structure and function, and it has impor tant practical applications in both biotechnology and biomedicine, as evidenced by the current intense interest in gene therapy. Although DNA and other macromolecules may be introduced into cells by a variety of methods, including chemical treatments and microinjection, el- troporation has proven to be simpler to perform, more efficient, and effective with a wider variety of cell types than other techniques. The early and broad success of electric field-mediated DNA transfer soon prompted researchers to investigate electroporation for transferring other types of molecules into cells, including RNA, enzymes, antibodies, and analytic dyes. Animal Cell Electroporation and Electrofusion Protocols begins with three chapters that describe the theoretical and practical aspects of electroporation, including a review of the commercially available instrumentation. These introductory chapters will be of particular inter est to those new to electric field technologies and to those developing protocols for as yet untested species or cell types. Nineteen chapters follow that present well-tested protocols for electroporation of proteins and DNA into insect, fish, and mammalian cells. |
| elisa theory and practice: Application of Solution Protein Chemistry to Biotechnology Roger L. Lundblad, 2009-05-12 Reflecting the versatility of the author's science and the depth of his experience, Application of Solution Protein Chemistry to Biotechnology explores key contributions that protein scientists can make in the development of products that are both important and commercially viable, and provides them with tools and information required for successfu |
| elisa theory and practice: PCR in Bioanalysis Stephen J Meltzer, 2008-02-03 PCR in Bioanalysis offers powerful PCR-based protocols and assays in actual use or potential use in clinical medicine and commercial biology. The main focus of the book is on the commercial applications of PCR, as opposed to basic research uses. Topics covered include the measurement of hormone levels using PCR, transcription factor isolation, detection of viruses using PCR, detection of tumor contamination of stem cells, evaluation of grafts for tumor cells, and more. |
| elisa theory and practice: cDNA Library Protocols Ian G. Cowell, Caroline A. Austin, 2008-02-02 The first libraries of complementary DNA (cDNA) clones were con structed in the mid-to-late 1970s using RNA-dependent DNA polymerase (reverse transcriptase) to convert poly A* mRNA into double-stranded cDNA suitable for insertion into prokaryotic vectors. Since then cDNA technology has become a fundamental tool for the molecular biologist and at the same time some very significant advances have occurred in the methods for con structing and screening cDNA libraries. It is not the aim of cDNA Library Protocols to give a comprehensive review of all cDNA library-based methodologies; instead we present a series of up-to-date protocols that together should give a good grounding of proce dures associated with the construction and use of cDNA libraries. In deciding what to include, we endeavored to combine up-to-date versions of some of the most widely used protocols with some very usefiil newer techniques. cDNA Library Protocols should therefore be especially useful to the investigator who is new to the use of cDNA libraries, but should also be of value to the more experienced worker. Chapters 1—5 concentrate on cDNA library construction and manipula tion, Chapters 6 and 7 describe means of cloning difficult-to-obtain ends of cDNAs, Chapters 8-18 give various approaches to the screening of cDNA libraries, and the remaining chapters present methods of analysis of cDNA clones including details of how to analyze cDNA sequence data and how to make use of the wealth of cDNA data emerging from the human genome project. |
| elisa theory and practice: Biochemicals and Reagents , |
| elisa theory and practice: Preclinical Development Handbook Shayne Cox Gad, 2008-03-14 A clear, straightforward resource to guide you through preclinical drug development Following this book's step-by-step guidance, you can successfully initiate and complete critical phases of preclinical drug development. The book serves as a basic, comprehensive reference to prioritizing and optimizing leads, dose formulation, ADME, pharmacokinetics, modeling, and regulations. This authoritative, easy-to-use resource covers all the issues that need to be considered and provides detailed instructions for current methods and techniques. Each chapter is written by one or more leading experts in the field. These authors, representing the many disciplines involved in preclinical toxicology screening and testing, give you the tools needed to apply an effective multidisciplinary approach. The editor has carefully reviewed all the chapters to ensure that each one is thorough, accurate, and clear. Among the key topics covered are: * Modeling and informatics in drug design * Bioanalytical chemistry * Absorption of drugs after oral administration * Transporter interactions in the ADME pathway of drugs * Metabolism kinetics * Mechanisms and consequences of drug-drug interactions Each chapter offers a full exploration of problems that may be encountered and their solutions. The authors also set forth the limitations of various methods and techniques used in determining the safety and efficacy of a drug during the preclinical stage. This publication should be readily accessible to all pharmaceutical scientists involved in preclinical testing, enabling them to perform and document preclinical safety tests to meet all FDA requirements before clinical trials may begin. |
| elisa theory and practice: Transmembrane Signaling Protocols Dafna Bar-Sagi, 1998 This collection of practical, cutting-edge techniques for the study of cell signaling provides detailed, step-by-step instructions, helpful notes, and troubleshooting tips that make even the most powerful of the newest techniques readily reproducible. The protocols presented include the use of peptide libraries to study transmembrane signaling; the use of single-cell assays to analyze signal transduction pathways; the reconstitution of signaling complexes; methods for analyzing protein-protein interactions, and more. Introductory reviews explain the basic theory and enable researchers new to the area to rapidly gain understanding, as well as command of the practical knowledge and expertise afforded by the protocols. Transmembrane Signaling Protocols makes available to all researchers the many state-of-the-art techniques that have recently led to landmark discoveries in transmembrane signaling. |
| elisa theory and practice: Plant Cell Electroporation And Electrofusion Protocols Jac A. Nickoloff, 2008-02-02 Gene transfer is an essential technology for improving our under standing of gene structure and function. Although there are many meth ods by which DNA may be introduced into cells—including heat and chemical treatments, and microinjection—electroporation has been found to be the most versatile gene transfer technique. Electroporation is effective with a wide variety of cell types, including those that are difficult to transform by other means. For many cell types, electroporation is either the most efficient or the only means known to effect gene transfer. The early and broad success of electric field-medi ated DNA transfer soon prompted researchers to investigate electroporation for transferring other types of molecules into cells, in cluding RNA, enzymes, antibodies, and analytic dyes. The first section of Plant Cell Electroporation and Electrofusion Protocols includes two chapters that serve as a guide to theoretical and practical aspects of electroporation, and will be of particular interest to those developing protocols for as yet untested species or cell types, and a third chapter that describes commercially available electroporation instruments. The remaining chapters describe well-tested protocols for DNA electrotransfection, electroporation of other biomolecules, or cell electrofusion. These chapters also include brief discussions of alterna tives to electric field-based methods, citing the advantages and limita tions of the various methods for achieving specific goals. |
| elisa theory and practice: No One Home Daniel Touro Linger, 2001 This is an ethnographic study, based on fieldwork and extensive personal interviews, of Brazilians of Japanese descent who have migrated to Japan in response to the government's call for ethnically acceptable unskilled workers. These people of Toyota City are among 200,000 Brazilians of Japanese descent who live in Japan today, forming Japan's third-largest minority group. |
| elisa theory and practice: Becoming a Translator Douglas Robinson, 2019-12-06 Fusing theory with advice and information about the practicalities of translating, Becoming a Translator is the essential resource for novice and practicing translators. The book explains how the market works, helps translators learn how to translate faster and more accurately, as well as providing invaluable advice and tips about how to deal with potential problems, such as stress. The fourth edition has been revised and updated throughout, offering: a whole new chapter on multimedia translation, with a discussion of the move from intersemiotic translation to audiovisual translation, media access and accessibility studies new sections on cognitive translation studies, translation technology, online translator communities, crowd-sourced translation, and online ethnography tweetstorms capturing the best advice from top industry professionals on Twitter student voices, especially from Greater China Including suggestions for discussion, activities, and hints for the teaching of translation, and drawing on detailed advice from top translation professionals, the fourth edition of Becoming a Translator remains invaluable for students and teachers of Translation Studies, as well as those working in the field of translation. |
| elisa theory and practice: Towards Livestock Disease Diagnosis and Control in the 21st Century International Atomic Energy Agency, 1998 Proceedings of a symposium on Diagnosis and Control of Livestock Diseases Using Nuclear and Related Techniques jointly organized by the IAEA and FAO, Vienna, 7-11 April 1997. The purpose of the symposium was to consider the application of science to livestock production as a complex of socioeconomic problems. It dealt not only with the impact of developments in serology and molecular biology, but also with questions of epidemiology, vaccines, information networks, geographical information systems and socioeconomic factors. |
| elisa theory and practice: Molecular Data Analysis Using R Csaba Ortutay, Zsuzsanna Ortutay, 2016-12-29 This book addresses the difficulties experienced by wet lab researchers with the statistical analysis of molecular biology related data. The authors explain how to use R and Bioconductor for the analysis of experimental data in the field of molecular biology. The content is based upon two university courses for bioinformatics and experimental biology students (Biological Data Analysis with R and High-throughput Data Analysis with R). The material is divided into chapters based upon the experimental methods used in the laboratories. Key features include: • Broad appeal--the authors target their material to researchers in several levels, ensuring that the basics are always covered. • First book to explain how to use R and Bioconductor for the analysis of several types of experimental data in the field of molecular biology. • Focuses on R and Bioconductor, which are widely used for data analysis. One great benefit of R and Bioconductor is that there is a vast user community and very active discussion in place, in addition to the practice of sharing codes. Further, R is the platform for implementing new analysis approaches, therefore novel methods are available early for R users. |
| elisa theory and practice: AutoImmune Blistering Disease Part I, An Issue of Dermatologic Clinics Dédée F. Murrell, 2011-04-28 In the first volume of this two part issue devoted to Autoimmune Blistering Diseases (AIBD), diagnosis and clinical features of these diseases are covered by an internationally recognized group of authors. Topics include Pemphigus Vulgaris, Pemphigus Foliaceus, Linear IgA Bullous Disease, and Hair and Nail Loss in AIBD. |
| elisa theory and practice: The Role of New Technologies in Medical Microbiological Research and Diagnosis John P. Hays, W. B. Van Leeuwen, 2012 This e-book provides a comprehensive overview of state of the art applications of biomolecular techniques that are currently used, or are in development in the field of microbiological diagnostics research. In this respect, the topics covered include, genomics, proteomics, immunologics, biosensors, microarrays and nano-culture technologies. The broad range of techniques covered by the book will be invaluable to readers from professions allied to (but not exclusive to) microbiology analysts and researchers, laboratory technicians, (medical) microbiologists, molecular biologists, analytical phys. |
ELISA - Wikipedia
The enzyme-linked immunosorbent assay (ELISA) (/ ɪ ˈ l aɪ z ə /, / ˌ iː ˈ l aɪ z ə /) is a commonly used analytical biochemistry assay, first described by Eva Engvall and Peter Perlmann in …
ELISA: What It Is, Purpose, Procedure & Results - Cleveland Clinic
ELISA is a laboratory technique that detects certain antibodies, antigens, proteins and hormones in bodily fluids. Tests for HIV and Lyme disease use ELISA. Learn the science behind several …
Overview of ELISA | Thermo Fisher Scientific - US
Learn about the different methods for performing an ELISA assay for protein quantitation, including assay design strategies and reagents.
An overview of ELISA: a review and update on best laboratory …
The enzyme-linked immunosorbent assay (ELISA) detects antigen-antibody interactions by using enzyme-labelled conjugates and enzyme substrates that generate colour changes. This …
ELISA : Principle, Procedure, Types, Applications and Animation
Mar 7, 2022 · Enzyme Linked Immunosorbent Assay (ELISA) is a very sensitive immunochemical technique which is used to access the presence of specific protein (antigen or antibody) in the …
Enzyme-linked immunosorbent assay (ELISA) - Microbe Notes
May 17, 2022 · Enzyme-Linked Immunosorbent Assay (ELISA) is a modern molecular technique for the detection of antigen-antibody interaction with the help of an enzyme. It is one of the …
What is an ELISA? - Abcam
ELISA is a type of immunological assay used to detect and quantify specific biomolecules, such as proteins, antigens, or antibodies, in biological samples. An ELISA, like other types of …
Enzyme-linked immunosorbent assay (ELISA) | British Society …
The enzyme-linked immunosorbent assay (ELISA) is an immunological assay commonly used to measure antibodies, antigens, proteins and glycoproteins in biological samples. Some …
ELISA- Definition, Principle, Procedure, Types, Steps, Applications
Aug 7, 2022 · ELISA (enzyme-linked immunosorbent assay) is a technique for detecting the presence of antigens in biological materials. An ELISA, like other types of immunoassays, …
What Is ELISA? - BYJU'S
ELISA is the basic assay technique, known as enzyme-linked immunosorbent assay (also referred to as EIA: Enzyme Immunoassay) that is carried out to detect and measure antibodies, …
ELISA - Wikipedia
The enzyme-linked immunosorbent assay (ELISA) (/ ɪ ˈ l aɪ z ə /, / ˌ iː ˈ l aɪ z ə /) is a commonly used analytical biochemistry assay, first described by Eva Engvall and Peter Perlmann in …
ELISA: What It Is, Purpose, Procedure & Results - Cleveland Clinic
ELISA is a laboratory technique that detects certain antibodies, antigens, proteins and hormones in bodily fluids. Tests for HIV and Lyme disease use ELISA. Learn the science behind several …
Overview of ELISA | Thermo Fisher Scientific - US
Learn about the different methods for performing an ELISA assay for protein quantitation, including assay design strategies and reagents.
An overview of ELISA: a review and update on best laboratory …
The enzyme-linked immunosorbent assay (ELISA) detects antigen-antibody interactions by using enzyme-labelled conjugates and enzyme substrates that generate colour changes. This …
ELISA : Principle, Procedure, Types, Applications and Animation
Mar 7, 2022 · Enzyme Linked Immunosorbent Assay (ELISA) is a very sensitive immunochemical technique which is used to access the presence of specific protein (antigen or antibody) in the …
Enzyme-linked immunosorbent assay (ELISA) - Microbe Notes
May 17, 2022 · Enzyme-Linked Immunosorbent Assay (ELISA) is a modern molecular technique for the detection of antigen-antibody interaction with the help of an enzyme. It is one of the …
What is an ELISA? - Abcam
ELISA is a type of immunological assay used to detect and quantify specific biomolecules, such as proteins, antigens, or antibodies, in biological samples. An ELISA, like other types of …
Enzyme-linked immunosorbent assay (ELISA) | British Society …
The enzyme-linked immunosorbent assay (ELISA) is an immunological assay commonly used to measure antibodies, antigens, proteins and glycoproteins in biological samples. Some …
ELISA- Definition, Principle, Procedure, Types, Steps, Applications
Aug 7, 2022 · ELISA (enzyme-linked immunosorbent assay) is a technique for detecting the presence of antigens in biological materials. An ELISA, like other types of immunoassays, …
What Is ELISA? - BYJU'S
ELISA is the basic assay technique, known as enzyme-linked immunosorbent assay (also referred to as EIA: Enzyme Immunoassay) that is carried out to detect and measure antibodies, …
