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| enzyme kinetics textbook: Enzyme Kinetics Hans Bisswanger, 2017-06-22 Now in full color for a more intuitive learning experience, this new edition of the long-selling reference also features a number of new developments in methodology and the application of enzyme kinetics. Starting with a description of ligand binding equilibria, the experienced author goes on to discuss simple and complex enzyme reactions in kinetic terms. Special cases such as membrane-bound and immobilized enzymes are considered, as is the influence of external conditions, such as temperature and pH value. The final part of the book then covers a range of widely used measurement methods and compares their performance and scope of application. With its unique mix of theory and practical advice, this is an invaluable aid for teaching as well as for experimental work. |
| enzyme kinetics textbook: Fundamentals of Enzyme Kinetics Athel Cornish-Bowden, 2013-02-22 Now in its fourth edition, this textbook is one of the few titles worldwide to cover enzyme kinetics in its entire scope and the only one to include its implications for bioinformatics and systems biology. Multi-enzyme complexes and cooperativity are therefore treated in more detail than in any other textbook on the market. The respected and well known author is one of the most experienced researchers into the topic and writes with outstanding style and didactic clarity. As with the previous editions, he presents here steady-state kinetics and fast reactions, supplementing each chapter with problems and solutions. For the first time, this edition features a companion website providing all figures in colour www.wiley-vch.de/home/fundenzykinet |
| enzyme kinetics textbook: Enzyme Kinetics: Catalysis and Control Daniel L. Purich, 2010-06-16 Far more than a comprehensive treatise on initial-rate and fast-reaction kinetics, this one-of-a-kind desk reference places enzyme science in the fuller context of the organic, inorganic, and physical chemical processes occurring within enzyme active sites. Drawing on 2600 references, Enzyme Kinetics: Catalysis & Control develops all the kinetic tools needed to define enzyme catalysis, spanning the entire spectrum (from the basics of chemical kinetics and practical advice on rate measurement, to the very latest work on single-molecule kinetics and mechanoenzyme force generation), while also focusing on the persuasive power of kinetic isotope effects, the design of high-potency drugs, and the behavior of regulatory enzymes. - Historical analysis of kinetic principles including advanced enzyme science - Provides both theoretical and practical measurements tools - Coverage of single molecular kinetics - Examination of force generation mechanisms - Discussion of organic and inorganic enzyme reactions |
| enzyme kinetics textbook: Enzyme Kinetics Alejandro G. Marangoni, 2003-04-23 Practical Enzyme Kinetics provides a practical how-to guide for beginning students, technicians, and non-specialists for evaluating enzyme kinetics using common software packages to perform easy enzymatic analyses. |
| enzyme kinetics textbook: Comprehensive Enzyme Kinetics Vladimir Leskovac, 2007-05-08 Welcome to your study of enzyme kinetics, the subject that underlies all enzymology, which in turn underlies all aspects of biochemistry. This text will give you an introduction to a wide range of topics that constitute the modern enzyme kinetics. This textbook is directed at graduate students in biochemistry, chemistry, and life sciences, for advanced courses in enzyme kinetics, enzymology, and enzyme chemistry. For this reason, the whole book is organized in a systematic and scholarly fashion. It is unlikely that the student will be expected to cover everything in the text, but in a later career she or he may find it an invaluable reference for topics that are needed in practice. The concepts, definitions and detailed algebra of enzyme kinetics are laid out in accurate detail. For that reason, this textbook can also serve as a handbook for enzyme kinetics for research workers in the field. The research worker will find it a useful source, which can be used for solving the daily experimental problems in the laboratory. The preparation of the manuscript for this book was under the constant surveillance of W. Wallace Cleland, Professor of Chemical Science at the University of Wisconsin in Madison, and one of the founders of modern enzyme kinetics. Without his help and advice, this bookwould not be possible. Several versions of the manuscript were constantly corrected and improved by Svetlana Professor of Biochemistry at the University of Novi Sad. |
| enzyme kinetics textbook: Enzyme Kinetics Paul C. Engel, 2013-11-21 The student of biological science in his final years as an undergraduate and his first years as a graduate is expected to gain some familiarity with current research at the fron tiers of his discipline. New research work is published in a perplexing diversity of publications and is inevitably concerned with the minutiae of the subject. The sheer number of research journals and papers also causes confusion and difficulties of assimilation. Review articles usually presuppose a background knowledge of the field and are inevitably rather restricted in scope. There is thus a need for short but authoritative introductions to those areas of modern biological research which are either not dealt with in standard introductory text books or are not dealt with in sufficient detail to enable the student to go on from them to read scholarly reviews with profit. This series of books is designed to satisfy this need. The authors have been asked to produce abrief outline of their subject assuming that their readers will have read and remembered much of a standard introductory textbook of biology. |
| enzyme kinetics textbook: Contemporary Enzyme Kinetics and Mechanism Daniel L. Purich, 1983-01-01 Selected Methods in Enzymology: Contemporary Enzyme Kinetics and Mechanism provides an introduction to enzyme kinetics and mechanism at an intermediate level. This book covers a variety of topics, including temperature effects in enzyme kinetics, cryoenzymology, substrate inhibition, enol intermediates enzymology, and heavy-atom isotope effects. Organized into 19 chapters, this book begins with an overview of derivation of rate equations as an integral part of the effective usage of kinetics as a tool. This text then examines the practical aspects of initial rate enzyme assay. Other chapters consider the basic procedures used in making decisions concerning kinetic mechanisms from initial-rate data. This book discusses as well the various aspects of both the theoretical background and the applications. The final chapter deals with the importance of achieving proficiency in formulating quantitative relationships describing enzyme behavior. This book is a valuable resource for students and research workers. Enzymologists and chemists will also find this book useful. |
| enzyme kinetics textbook: Enzyme Kinetics and Mechanism Paul F. Cook, W. W. Cleland, 2007-03-06 Enzyme Kinetics and Mechanism is a comprehensive textbook on steady-state enzyme kinetics. Organized according to the experimental process, the text covers kinetic mechanism, relative rates of steps along the reaction pathway, and chemical mechanism—including acid-base chemistry and transition state structure. Practical examples taken from the literature demonstrate theory throughout. The book also features numerous general experimental protocols and how-to explanations for interpreting kinetic data. Written in clear, accessible language, the book will enable graduate students well-versed in biochemistry to understand and describe data at the fundamental level. Enzymologists and molecular biologists will find the text a useful reference. |
| enzyme kinetics textbook: Enzyme Kinetics Irwin H. Segel, 1993-05-06 Covers enzyme kinetics from its most elementary aspects to such modern subjects as steady-state, multi-reactant kinetics and isotope exchange. Offers an understanding of the behavior of enzyme systems and the diagnostic tools used to characterize them and determine kinetic mechanisms. Illustrates and explains current subjects such as cumulative, concerted and cooperative feedback inhibition and metal ion activation. |
| enzyme kinetics textbook: Enzyme Kinetics for Systems Biology Herbert M. Sauro, 2011 This book introduces fundamental concepts in kinetics that relate to system biology. Topics include basic kinetics, enzyme kinetics, generalized rate laws, gene regulation and introduction to elasticities. The text is suitable for junior/senior undergraduates and graduates who need access to information relevant to modeling biochemical pathways. |
| enzyme kinetics textbook: Enzyme Kinetics in Drug Metabolism Swati Nagar, Upendra A. Argikar, Donald J. Tweedie, 2014-02-13 Drug metabolism and transport are very important facets within the discipline of pharmaceutical sciences, with enzyme kinetic concepts utilized regularly in characterizing and modeling the disposition and elimination of drugs. Enzyme Kinetics in Drug Metabolism: Fundamentals and Applications focuses on very practical aspects of applying kinetic principles to drug metabolizing enzymes and transporters. Divided into five convenient sections, topics include the fundamental principles of enzyme kinetics, the kinetics of oxidative and conjugative drug metabolizing enzymes and drug transporters, modeling approaches for both drug metabolizing enzymes and transporters including novel systems biology approaches, understanding of variability both experimental and interindividual (pharmacogenomic), and case studies that provide real life examples of applying these principles. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics especially suitable for the novice, in some cases step-by-step, readily reproducible protocols, and insights to help with troubleshooting and avoiding known pitfalls with extensive cross referencing to assist in learning. Authoritative and easily accessible, Enzyme Kinetics in Drug Metabolism: Fundamentals and Applications serves as a very practical teaching tool for novice, non-mathematically trained scientists interested in these fundamental concepts and as an aid for their supervisors in teaching these principles. |
| enzyme kinetics textbook: Enzyme Catalysis and Regulation Gorden Hammes, 2012-12-02 Enzyme Catalysis and Regulation is an introduction to enzyme catalysis and regulation and covers topics ranging from protein structure and dynamics to steady-state enzyme kinetics, multienzyme complexes, and membrane-bound enzymes. Case studies of selected enzyme mechanisms are also presented. This book consists of 11 chapters and begins with a brief overview of enzyme structure, followed by a discussion on methods of probing enzyme structure such as X-ray crystallography and optical spectroscopy. Kinetic methods are then described, with emphasis on the general principles of steady-state and transient kinetics. The chemical principles involved in enzyme catalysis are also discussed, and case studies of a few well-documented enzymes are presented. The regulation of enzyme activity is analyzed from a nongenetic viewpoint, with particular reference to binding isotherms and models for allosterism. Two particular enzymes, aspartate transcarbamoylase and phosphofructokinase, are used as examples of well-studied regulatory enzymes. The last two chapters focus on multienzyme complexes and membrane-bound enzymes. This monograph is intended for graduate students, advanced undergraduates, and research workers in molecular biology and biochemistry. |
| enzyme kinetics textbook: Steady-state Enzyme Kinetics Stanley Ainsworth, 1977 |
| enzyme kinetics textbook: Catalytic Kinetics Dmitry Yu Murzin, Tapio Salmi, 2016-06-04 Catalytic Kinetics: Chemistry and Engineering, Second Edition offers a unified view that homogeneous, heterogeneous, and enzymatic catalysis form the cornerstone of practical catalysis. The book has an integrated, cross-disciplinary approach to kinetics and transport phenomena in catalysis, but still recognizes the fundamental differences between different types of catalysis. In addition, the book focuses on a quantitative chemical understanding and links the mathematical approach to kinetics with chemistry. A diverse group of catalysts is covered, including catalysis by acids, organometallic complexes, solid inorganic materials, and enzymes, and this fully updated second edition contains a new chapter on the concepts of cascade catalysis. Finally, expanded content in this edition provides more in-depth discussion, including topics such as organocatalysis, enzymatic kinetics, nonlinear dynamics, solvent effects, nanokinetics, and kinetic isotope effects. - Fully revised and expanded, providing the latest developments in catalytic kinetics - Bridges the gaps that exist between hetero-, homo- and enzymatic-catalysis - Provides necessary tools and new concepts for researchers already working in the field of catalytic kinetics - Written by internationally-renowned experts in the field - Examples and exercises following each chapter make it suitable as an advanced course book |
| enzyme kinetics textbook: Biomolecular Kinetics Clive R. Bagshaw, 2017-10-04 a gem of a textbook which manages to produce a genuinely fresh, concise yet comprehensive guide –Mark Leake, University of York destined to become a standard reference.... Not just a ‘how to’ handbook but also an accessible primer in the essentials of kinetic theory and practice. –Michael Geeves, University of Kent covers the entire spectrum of approaches, from the traditional steady state methods to a thorough account of transient kinetics and rapid reaction techniques, and then on to the new single molecule techniques –Stephen Halford, University of Bristol This illustrated treatment explains the methods used for measuring how much a reaction gets speeded up, as well as the framework for solving problems such as ligand binding and macromolecular folding, using the step-by-step approach of numerical integration. It is a thoroughly modern text, reflecting the recent ability to observe reactions at the single-molecule level, as well as advances in microfluidics which have given rise to femtoscale studies. Kinetics is more important now than ever, and this book is a vibrant and approachable entry for anyone who wants to understand mechanism using transient or single molecule kinetics without getting bogged down in advanced mathematics. Clive R. Bagshaw is Emeritus Professor at the University of Leicester, U.K., and Research Associate at the University of California at Santa Cruz, U.S.A. |
| enzyme kinetics textbook: Enzymes Robert A. Copeland, 2004-04-07 Fully updated and expanded-a solid foundation for understandingexperimental enzymology. This practical, up-to-date survey is designed for a broadspectrum of biological and chemical scientists who are beginning todelve into modern enzymology. Enzymes, Second Editionexplains the structural complexities of proteins and enzymes andthe mechanisms by which enzymes perform their catalytic functions.The book provides illustrative examples from the contemporaryliterature to guide the reader through concepts and data analysisprocedures. Clear, well-written descriptions simplify the complexmathematical treatment of enzyme kinetic data, and numerouscitations at the end of each chapter enable the reader to accessthe primary literature and more in-depth treatments of specifictopics. This Second Edition of Enzymes: A Practical Introductionto Structure, Mechanism, and Data Analysis features refinedand expanded coverage of many concepts, while retaining theintroductory nature of the book. Important new featuresinclude: A new chapter on protein-ligand binding equilibria Expanded coverage of chemical mechanisms in enzyme catalysisand experimental measurements of enzyme activity Updated and refined discussions of enzyme inhibitors andmultiple substrate reactions Coverage of current practical applications to the study ofenzymology Supplemented with appendices providing contact information forsuppliers of reagents and equipment for enzyme studies, as well asa survey of useful Internet sites and computer software forenzymatic data analysis, Enzymes, Second Edition isthe ultimate practical guide for scientists and students inbiochemical, pharmaceutical, biotechnical, medicinal, andagricultural/food-related research. |
| enzyme kinetics textbook: Enzyme Kinetics Arthur R. Schulz, 1995-01-26 This textbook for advanced courses in enzyme chemistry and enzyme kinetics covers the field of steady-state enzyme kinetics from the basic principles inherent in the Henri equation to the expressions that describe the control of multi-enzyme pathways. Steady-state kinetic equations are derived with the use of the connection matrix method, and an algorithm is developed that can be implemented easily for computer-based derivation of the equations. Throughout the book emphasis is placed on a proper interpretation of the kinetic behavior of multi-reactant enzymic reactions and on the interpretation of control coefficients in terms of metabolic control. Problems are included at the end of each chapter and their solutions are found at the end of the book. |
| enzyme kinetics textbook: Principles of Enzyme Kinetics Athel Cornish-Bowden, 2014-05-20 Principles of Enzyme Kinetics discusses the principles of enzyme kinetics at an intermediate level. It is primarily written for first-year research students in enzyme kinetics. The book is composed of 10 chapters. Chapter 1 provides the basic principles of enzyme kinetics with a brief discussion of dimensional analysis. Subsequent chapters cover topics on the essential characteristics of steady-state kinetics, temperature dependence, methods for deriving steady-state rate equations, and control of enzyme activity. Integrated rate equations, and introductions to the study of fast reactions and the statistical aspects of enzyme kinetics are provided as well. Chemists and biochemists will find the book invaluable. |
| enzyme kinetics textbook: Organic Chemistry of Enzyme-Catalyzed Reactions, Revised Edition Richard B. Silverman, 2002-03-07 The Organic Chemistry of Enzyme-Catalyzed Reactions is not a book on enzymes, but rather a book on the general mechanisms involved in chemical reactions involving enzymes. An enzyme is a protein molecule in a plant or animal that causes specific reactions without itself being permanently altered or destroyed. This is a revised edition of a very successful book, which appeals to both academic and industrial markets. - Illustrates the organic mechanism associated with each enzyme-catalyzed reaction - Makes the connection between organic reaction mechanisms and enzyme mechanisms - Compiles the latest information about molecular mechanisms of enzyme reactions - Accompanied by clearly drawn structures, schemes, and figures - Includes an extensive bibliography on enzyme mechanisms covering the last 30 years - Explains how enzymes can accelerate the rates of chemical reactions with high specificity - Provides approaches to the design of inhibitors of enzyme-catalyzed reactions - Categorizes the cofactors that are appropriate for catalyzing different classes of reactions - Shows how chemical enzyme models are used for mechanistic studies - Describes catalytic antibody design and mechanism - Includes problem sets and solutions for each chapter - Written in an informal and didactic style |
| enzyme kinetics textbook: Enzyme Biocatalysis Andrés Illanes, 2008-06-19 This book was written with the purpose of providing a sound basis for the design of enzymatic reactions based on kinetic principles, but also to give an updated vision of the potentials and limitations of biocatalysis, especially with respect to recent app- cations in processes of organic synthesis. The ?rst ?ve chapters are structured in the form of a textbook, going from the basic principles of enzyme structure and fu- tion to reactor design for homogeneous systems with soluble enzymes and hete- geneous systems with immobilized enzymes. The last chapter of the book is divided into six sections that represent illustrative case studies of biocatalytic processes of industrial relevance or potential, written by experts in the respective ?elds. We sincerely hope that this book will represent an element in the toolbox of gr- uate students in applied biology and chemical and biochemical engineering and also of undergraduate students with formal training in organic chemistry, biochemistry, thermodynamics and chemical reaction kinetics. Beyond that, the book pretends also to illustrate the potential of biocatalytic processes with case studies in the ?eld of organic synthesis, which we hope will be of interest for the academia and prof- sionals involved in R&D&I. If some of our young readers are encouraged to engage or persevere in their work in biocatalysis this will certainly be our more precious reward. |
| enzyme kinetics textbook: Numerical Methods for the Life Scientist Heino Prinz, 2011-08-06 Enzyme kinetics, binding kinetics and pharmacological dose-response curves are currently analyzed by a few standard methods. Some of these, like Michaelis-Menten enzyme kinetics, use plausible approximations, others, like Hill equations for dose-response curves, are outdated. Calculating realistic reaction schemes requires numerical mathematical routines which usually are not covered in the curricula of life science. This textbook will give a step-by-step introduction to numerical solutions of non-linear and differential equations. It will be accompanied with a set of programs to calculate any reaction scheme on any personal computer. Typical examples from analytical biochemistry and pharmacology can be used as versatile templates. When a reaction scheme is applied for data fitting, the resulting parameters may not be unique. Correlation of parameters will be discussed and simplification strategies will be offered. |
| enzyme kinetics textbook: Enzyme Kinetics for Systems Biology Ambrosius Publishing, 2011-03-30 This book introduces fundamental concepts in kinetics that relate to system biology. The text is suitable for junior/senior undergraduates and graduates who need access to information relevant to modeling biochemical pathways. |
| enzyme kinetics textbook: A Textbook of Physical Chemistry – Volume 1 Mandeep Dalal, 2018-01-01 An advanced-level textbook of physical chemistry for the graduate (B.Sc) and postgraduate (M.Sc) students of Indian and foreign universities. This book is a part of four volume series, entitled A Textbook of Physical Chemistry – Volume I, II, III, IV. CONTENTS: Chapter 1. Quantum Mechanics – I: Postulates of quantum mechanics; Derivation of Schrodinger wave equation; Max-Born interpretation of wave functions; The Heisenberg’s uncertainty principle; Quantum mechanical operators and their commutation relations; Hermitian operators (elementary ideas, quantum mechanical operator for linear momentum, angular momentum and energy as Hermition operator); The average value of the square of Hermitian operators; Commuting operators and uncertainty principle(x & p; E & t); Schrodinger wave equation for a particle in one dimensional box; Evaluation of average position, average momentum and determination of uncertainty in position and momentum and hence Heisenberg’s uncertainty principle; Pictorial representation of the wave equation of a particle in one dimensional box and its influence on the kinetic energy of the particle in each successive quantum level; Lowest energy of the particle. Chapter 2. Thermodynamics – I: Brief resume of first and second Law of thermodynamics; Entropy changes in reversible and irreversible processes; Variation of entropy with temperature, pressure and volume; Entropy concept as a measure of unavailable energy and criteria for the spontaneity of reaction; Free energy, enthalpy functions and their significance, criteria for spontaneity of a process; Partial molar quantities (free energy, volume, heat concept); Gibb’s-Duhem equation. Chapter 3. Chemical Dynamics – I: Effect of temperature on reaction rates; Rate law for opposing reactions of Ist order and IInd order; Rate law for consecutive & parallel reactions of Ist order reactions; Collision theory of reaction rates and its limitations; Steric factor; Activated complex theory; Ionic reactions: single and double sphere models; Influence of solvent and ionic strength; The comparison of collision and activated complex theory. Chapter 4. Electrochemistry – I: Ion-Ion Interactions: The Debye-Huckel theory of ion- ion interactions; Potential and excess charge density as a function of distance from the central ion; Debye Huckel reciprocal length; Ionic cloud and its contribution to the total potential; Debye - Huckel limiting law of activity coefficients and its limitations; Ion-size effect on potential; Ion-size parameter and the theoretical mean-activity coefficient in the case of ionic clouds with finite-sized ions; Debye - Huckel-Onsager treatment for aqueous solutions and its limitations; Debye-Huckel-Onsager theory for non-aqueous solutions; The solvent effect on the mobality at infinite dilution; Equivalent conductivity (Λ) vs. concentration c 1/2 as a function of the solvent; Effect of ion association upon conductivity (Debye- Huckel - Bjerrum equation). Chapter 5. Quantum Mechanics – II: Schrodinger wave equation for a particle in a three dimensional box; The concept of degeneracy among energy levels for a particle in three dimensional box; Schrodinger wave equation for a linear harmonic oscillator & its solution by polynomial method; Zero point energy of a particle possessing harmonic motion and its consequence; Schrodinger wave equation for three dimensional Rigid rotator; Energy of rigid rotator; Space quantization; Schrodinger wave equation for hydrogen atom, separation of variable in polar spherical coordinates and its solution; Principle, azimuthal and magnetic quantum numbers and the magnitude of their values; Probability distribution function; Radial distribution function; Shape of atomic orbitals (s,p & d). Chapter 6. Thermodynamics – II: Classius-Clayperon equation; Law of mass action and its thermodynamic derivation; Third law of thermodynamics (Nernest heat theorem, determination of absolute entropy, unattainability of absolute zero) and its limitation; Phase diagram for two completely miscible components systems; Eutectic systems, Calculation of eutectic point; Systems forming solid compounds Ax By with congruent and incongruent melting points; Phase diagram and thermodynamic treatment of solid solutions. Chapter 7. Chemical Dynamics – II: Chain reactions: hydrogen-bromine reaction, pyrolysis of acetaldehyde, decomposition of ethane; Photochemical reactions (hydrogen - bromine & hydrogen -chlorine reactions); General treatment of chain reactions (ortho-para hydrogen conversion and hydrogen - bromine reactions); Apparent activation energy of chain reactions, Chain length; Rice-Herzfeld mechanism of organic molecules decomposition(acetaldehyde); Branching chain reactions and explosions ( H2-O2 reaction); Kinetics of (one intermediate) enzymatic reaction : Michaelis-Menton treatment; Evaluation of Michaelis 's constant for enzyme-substrate binding by Lineweaver-Burk plot and Eadie-Hofstae methods; Competitive and non-competitive inhibition. Chapter 8. Electrochemistry – II: Ion Transport in Solutions: Ionic movement under the influence of an electric field; Mobility of ions; Ionic drift velocity and its relation with current density; Einstein relation between the absolute mobility and diffusion coefficient; The Stokes- Einstein relation; The Nernst -Einstein equation; Walden’s rule; The Rate-process approach to ionic migration; The Rate process equation for equivalent conductivity; Total driving force for ionic transport, Nernst - Planck Flux equation; Ionic drift and diffusion potential; the Onsager phenomenological equations; The basic equation for the diffusion; Planck-Henderson equation for the diffusion potential. |
| enzyme kinetics textbook: Bioprocess Engineering Shijie Liu, 2012-11-07 Bioprocess Engineering involves the design and development of equipment and processes for the manufacturing of products such as food, feed, pharmaceuticals, nutraceuticals, chemicals, and polymers and paper from biological materials. It also deals with studying various biotechnological processes. Bioprocess Kinetics and Systems Engineering first of its kind contains systematic and comprehensive content on bioprocess kinetics, bioprocess systems, sustainability and reaction engineering. Dr. Shijie Liu reviews the relevant fundamentals of chemical kinetics-including batch and continuous reactors, biochemistry, microbiology, molecular biology, reaction engineering, and bioprocess systems engineering- introducing key principles that enable bioprocess engineers to engage in the analysis, optimization, design and consistent control over biological and chemical transformations. The quantitative treatment of bioprocesses is the central theme of this book, while more advanced techniques and applications are covered with some depth. Many theoretical derivations and simplifications are used to demonstrate how empirical kinetic models are applicable to complicated bioprocess systems. Contains extensive illustrative drawings which make the understanding of the subject easy Contains worked examples of the various process parameters, their significance and their specific practical use Provides the theory of bioprocess kinetics from simple concepts to complex metabolic pathways Incorporates sustainability concepts into the various bioprocesses |
| enzyme kinetics textbook: Essentials of Biochemistry Herbert J. Fromm, Mark Hargrove, 2011-12-23 This textbook, Essentials of Biochemistry is aimed at chemistry and biochemistry undergraduate students and first year biochemistry graduate students. It incorporates the lectures of the authors given to students with a strong chemistry background. An emphasis is placed on metabolism and reaction mechanisms and how they are studied. As the title of the book implies, the text lays the basis for an understanding of the fundamentals of biochemistry. |
| enzyme kinetics textbook: An Introduction to Chemical Kinetics Michel Soustelle, 2013-02-07 This book is a progressive presentation of kinetics of the chemical reactions. It provides complete coverage of the domain of chemical kinetics, which is necessary for the various future users in the fields of Chemistry, Physical Chemistry, Materials Science, Chemical Engineering, Macromolecular Chemistry and Combustion. It will help them to understand the most sophisticated knowledge of their future job area. Over 15 chapters, this book present the fundamentals of chemical kinetics, its relations with reaction mechanisms and kinetic properties. Two chapters are then devoted to experimental results and how to calculate the kinetic laws in both homogeneous and heterogeneous systems. The following two chapters describe the main approximation modes to calculate these laws. Three chapters are devoted to elementary steps with the various classes, the principles used to write them and their modeling using the theory of the activated complex in gas and condensed phases. Three chapters are devoted to the particular areas of chemical reactions, chain reactions, catalysis and the stoichiometric heterogeneous reactions. Finally the non-steady-state processes of combustion and explosion are treated in the final chapter. |
| enzyme kinetics textbook: Fast Reactions Kenneth Kustin, 1969 Chemical relaxation. Electrochemistry. Rapid mexing. Irradiation. |
| enzyme kinetics textbook: Catalysis in Chemistry and Enzymology William P. Jencks, 1987-01-01 Exceptionally clear coverage of mechanisms for catalysis, forces in aqueous solution, carbonyl- and acyl-group reactions, practical kinetics, more. |
| enzyme kinetics textbook: Enzyme Kinetics and Mechanism Paul F. Cook, W. W. Cleland, 2007-03-06 Enzyme Kinetics and Mechanism is a comprehensive textbook on steady-state enzyme kinetics. Organized according to the experimental process, the text covers kinetic mechanism, relative rates of steps along the reaction pathway, and chemical mechanism—including acid-base chemistry and transition state structure. Practical examples taken from the literature demonstrate theory throughout. The book also features numerous general experimental protocols and how-to explanations for interpreting kinetic data. Written in clear, accessible language, the book will enable graduate students well-versed in biochemistry to understand and describe data at the fundamental level. Enzymologists and molecular biologists will find the text a useful reference. |
| enzyme kinetics textbook: Principles of Chemical Kinetics Gorden Hammes, 2012-12-02 Principles of Chemical Kinetics is devoted to the principles and applications of chemical kinetics. The phenomenology and commonly used theories of chemical kinetics are presented in a critical manner, with particular emphasis on collision dynamics. How and what mechanistic information can be obtained from various experimental approaches is stressed throughout this book. Comprised of nine chapters, this text opens with an overview of reaction rates and their empirical analysis, along with theories of chemical kinetics. The following chapters consider reactions and unimolecular decompositions in the gas phase; chemical reactions in molecular beams; and energy transfer and partitioning in chemical reactions. Kinetics in liquid solutions and fast reactions in liquids are also described. The final chapter looks at the kinetics of enzymes, with particular reference to steady state and transient state kinetics, the pH and temperature dependence of kinetic parameters, and the mechanism underlying enzymatic action. This monograph is intended for students with a general college background in chemistry, physics, and mathematics, and with a typical undergraduate course in physical chemistry. |
| enzyme kinetics textbook: Enzymology T. Devasena, 2010 Enzymology is designed as a full-fledge textbook for the undergraduate engineering students of Biotechnology and Chemical Engineering. In addition, this book would also serve as an invaluable reference for students who are pursuing their graduate and postgraduate degree programs in Biotechnology, and all other life sciences programs that offer a course on Enzymes. The book covers all the fundamental and inevitable concepts like Enzyme Kinetics, Enzyme Inhibition, Enzyme Activity Regulation and proceeds in to discussion of applications of enzymes in various domains including Molecular Biology, Cloning and Genetic Engineering. A separate chapter has been devoted to the study of Enzyme Engineering and Technology, which the engineering students would find useful. Comprehensive in its coverage of topics, the book is rich in features like illustrations supporting the theoretical discussion, chapter-end summary, glossary of important terms and review questions to reinforce the learning. Numerical problems too have been provided in all the relevant chapters. |
| enzyme kinetics textbook: Fundamentals of Protein Structure and Function Engelbert Buxbaum, 2015-11-27 This book serves as an introduction to protein structure and function. Starting with their makeup from simple building blocks, called amino acids, the 3-dimensional structure of proteins is explained. This leads to a discussion how misfolding of proteins causes diseases like cancer, various encephalopathies, or diabetes. Enzymology and modern concepts of enzyme kinetics are then introduced, taking into account the physiological, pharmacological and medical significance of this often neglected topic. This is followed by thorough coverage of hæmoglobin and myoglobin, immunoproteins, motor proteins and movement, cell-cell interactions, molecular chaperones and chaperonins, transport of proteins to various cell compartments and solute transport across biological membranes. Proteins in the laboratory are also covered, including a detailed description of the purification and determination of proteins, as well as their characterisation for size and shape, structure and molecular interactions. The book emphasises the link between protein structure, physiological function and medical significance. This book can be used for graduate and advanced undergraduate classes covering protein structure and function and as an introductory text for researchers in protein biochemistry, molecular and cell biology, chemistry, biophysics, biomedicine and related courses. About the author: Dr. Buxbaum is a biochemist with interest in enzymology and protein science. He has been working on the biochemistry of membrane transport proteins for nearly thirty years and has taught courses in biochemistry and biomedicine at several universities. |
| enzyme kinetics textbook: How Enzymes Work Haruo Suzuki, 2019-08-22 The first edition of this book covered the basic treatment of the enzyme reaction using the overall reaction kinetics and stopped-flow method, the general properties of protein and cofactors, the control of enzyme reaction, and the preparation of enzyme protein. These topics are the basis of enzyme research and thus suitable for the beginner in the field. The second edition presents the cofactors produced via the post-translational modification of the enzyme’s active site. These cofactors expand the function of enzymes and open a new research field. The carbonyl reagent phenylhydrazine and related compounds have been useful in finding some of the newly discovered cofactors and thus have been discussed in this edition. The topic of the control of enzyme activity through the channel of substrates and products in polyfunctional enzymes has also been expanded in this book. |
| enzyme kinetics textbook: Handbook of Biosensors and Biosensor Kinetics Ajit Sadana, Neeti Sadana, 2010-08-26 Biosensors are essential to an ever-expanding range of applications, including healthcare; drug design; detection of biological, chemical, and toxic agents; environmental monitoring; biotechnology; aviation; physics; oceanography; and the protection of civilian and engineering infrastructures. This book, like the previous five books on biosensors by this author (and one by the co-author), addresses the neglected areas of analyte-receptor binding and dissociation kinetics occurring on biosensor surfaces. Topics are covered in a comprehensive fashion, with homogeneous presentation for the benefit of the reader. The contributors address the economic aspects of biosensors and incorporate coverage of biosensor fabrication and nanobiosensors, among other topics. The comments, comparison, and discussion presented provides a better perspective of where the field of biosensors is heading. - Serves as a comprehensive resource on biosensor analysis - Examines timely topics such as biosensor fabrication and nanobiosensors - Covers economic aspects and medical applications (e.g., the role of analytes in controlling diabetes) |
| enzyme kinetics textbook: Biocatalysts and Enzyme Technology Klaus Buchholz, Volker Kasche, Uwe Theo Bornscheuer, 2012-12-21 This second edition of a bestselling textbook offers an instructive and comprehensive overview of our current knowledge of biocatalysis and enzyme technology. The book now contains about 40% more printed content. Three chapters are completely new, while the others have been thoroughly updated, and a section with problems and solutions as well as new case studies have been added. Following an introduction to the history of enzyme applications, the text goes on to cover in depth enzyme mechanisms and kinetics, production, recovery, characterization and design by protein engineering. The authors treat a broad range of applications of soluble and immobilized biocatalysts, including wholecell systems, the use of non-aqueous reaction systems, applications in organic synthesis, bioreactor design and reaction engineering. Methods to estimate the sustainability, important internet resources and their evaluation, and legislation concerning the use of biocatalysts are also covered. |
| enzyme kinetics textbook: Allosteric Regulatory Enzymes Thomas W. Traut, 2007-09-11 All enzymes are remarkable since they have the ability to increase the rate of a chemical reaction, often by more than a billion-fold. Allosteric enzymes are even more amazing because the have the additional ability to change their rate in response to cellular activators or inhibitors. This enables them to control the pathway in which they are the regulatory enzyme. Since the effector molecules represent the current status of the cell for a given metabolic pathway, this results in very responsive and balanced metabolic states, and makes it possible for cells and organisms to be appropriately dynamic, and responsive, in a changing environment. This book provides a logical introduction to the limits for enzyme function as dictated by the factors that are limits for life. This book presents a complete description of all the mechanisms used for changing enzyme acticity. Eight enzymes are used as model systems after extensive study of their mechanisms. Wherever possible, the human form of the enzyme is used to illustrate the regulatory features. |
| enzyme kinetics textbook: Practical Enzymology Hans Bisswanger, 2013-06-21 The second edition of the perfect companion for practical course work in enzymology. Now with an improved selection of enzymatic assays based on key metabolic reactions, this book provides the detailed background to the enzymatic reaction and the enzyme per se. Particular emphasis is placed on troubleshooting with the described methods, and, in addition, the new four-color layout throughout features detailed protocols highlighted for easy recognition. www.wiley-vch.de/home/enzymology: A companion website provides animations for all figures together with supplementary material, for deeper understanding of the partially abstract matter. |
| enzyme kinetics textbook: Introduction to Modeling Biological Cellular Control Systems Weijiu Liu, 2012-04-26 This textbook contains the essential knowledge in modeling, simulation, analysis, and applications in dealing with biological cellular control systems. In particular, the book shows how to use the law of mass balance and the law of mass action to derive an enzyme kinetic model - the Michaelis-Menten function or the Hill function, how to use a current-voltage relation, Nernst potential equilibrium equation, and Hodgkin and Huxley's models to model an ionic channel or pump, and how to use the law of mass balance to integrate these enzyme or channel models into a complete feedback control system. The book also illustrates how to use data to estimate parameters in a model, how to use MATLAB to solve a model numerically, how to do computer simulations, and how to provide model predictions. Furthermore, the book demonstrates how to conduct a stability and sensitivity analysis on a model. |
| enzyme kinetics textbook: Enzyme Technology : Pacemaker of Biotechnology Nooralabettu Krishna Prasad, 2011-04 Keeping in view the well-established place of enzymes in the field of biotechnology and the recent development in biotech industries, this comprehensive and well-written textbook presents the fundamental concepts of enzyme technology, emphasizing the practical and economic aspects of enzyme usage. Beginning with an overview of enzymes giving insights into the physicochemical properties, classifications, sources, mechanisms and characteristics of enzymes, the text discusses the enzyme kinetics in detail. It furnishes a great deal of information on potential of enzymes for their commercial exploitation. The text then goes on to describe the biotechnical significance of enzymes with their applications in the fields of food and pharmaceutical industries. The text is supported by a large number of solved examples and illustrative diagrams. Primarily designed for undergraduate and postgraduate students of biotechnology and biochemical engineering, the book will also be useful to professionals, researchers and entrepreneurs. KEY FEATURES: Written in an easy-to-understand style. Provides simple, clear and authoritative guide to the principles and scope of enzymes in biotechnology. Includes chapter-end review questions based on recently appeared university question papers. |
| enzyme kinetics textbook: Evaluation of Enzyme Inhibitors in Drug Discovery Robert A. Copeland, 2005-04-01 Vital information for discovering and optimizing new drugs Understanding the data and the experimental details that support it has always been at the heart of good science and the assumption challenging process that leads from good science to drug discovery. This book helps medicinal chemists and pharmacologists to do exactly that in the realm of enzyme inhibitors. -Paul S. Anderson, PhD This publication provides readers with a thorough understanding of enzyme-inhibitor evaluation to assist them in their efforts to discover and optimize novel drug therapies. Key topics such as competitive, noncompetitive, and uncompetitive inhibition, slow binding, tight binding, and the use of Hill coefficients to study reaction stoichiometry are all presented. Examples of key concepts are presented with an emphasis on clinical relevance and practical applications. Targeted to medicinal chemists and pharmacologists, Evaluation of Enzyme Inhibitors in Drug Discovery focuses on the questions that they need to address: * What opportunities for inhibitor interactions with enzyme targets arise from consideration of the catalytic reaction mechanism? * How are inhibitors evaluated for potency, selectivity, and mode of action? * What are the advantages and disadvantages of specific inhibition modalities with respect to efficacy in vivo? * What information do medicinal chemists and pharmacologists need from their biochemistry and enzymology colleagues to effectively pursue lead optimization? Beginning with a discussion of the advantages of enzymes as targets for drug discovery, the publication then explores the reaction mechanisms of enzyme catalysis and the types of interactions that can occur between enzymes and inhibitory molecules that lend themselves to therapeutic use. Next are discussions of mechanistic issues that must be considered when designing enzyme assays for compound library screening and for lead optimization efforts. Finally, the publication delves into special forms of inhibition that are commonly encountered in drug discovery efforts, but can be easily overlooked or misinterpreted. This publication is designed to provide students with a solid foundation in enzymology and its role in drug discovery. Medicinal chemists and pharmacologists can refer to individual chapters as specific issues arise during the course of their ongoing drug discovery efforts. |
Enzyme - Wikipedia
An enzyme (/ ˈ ɛ n z aɪ m /) is a protein that acts as a biological catalyst by accelerating chemical reactions. The molecules upon which enzymes may act are called substrates, and the enzyme …
Enzyme | Definition, Mechanisms, & Nomenclature | Britannica
May 20, 2025 · Enzyme, a catalyst that regulates the rate at which chemical reactions proceed in living organisms without itself being altered in the process. Most critically, enzymes catalyze all …
Enzymes: Function, definition, and examples - Medical News Today
Dec 8, 2023 · Enzymes provide help with facilitating chemical reactions within each cell. Since they are not destroyed during the process, a cell can reuse each enzyme repeatedly. This article...
Enzymes: What Are Enzymes, Pancreas, Digestion & Liver Function
May 12, 2021 · Enzymes are proteins that help speed up chemical reactions in our bodies. Enzymes are essential for digestion, liver function and much more. Too much or too little of a certain …
Enzymes: principles and biotechnological applications - PMC
Enzymes are biological catalysts (also known as biocatalysts) that speed up biochemical reactions in living organisms. They can also be extracted from cells and then used to catalyse a wide range …
Enzyme: Definition, Types, Structure, Functions, & Diagram
Nov 11, 2021 · Enzymes are protein macromolecules that are necessary to initiate or speed up the rate of chemical reactions in the bodies of living organisms. The molecules on which enzymes act …
Enzyme - National Human Genome Research Institute
4 days ago · An enzyme is a biological catalyst and is almost always a protein. It speeds up the rate of a specific chemical reaction in the cell. The enzyme is not destroyed during the reaction and is …
Enzymes - Definition, Examples, Function - Science Notes and …
Mar 25, 2025 · Enzymes are specialized proteins (and in some cases RNA molecules) that act as catalysts in living organisms. They speed up the chemical reactions required for life by lowering …
What Are Enzymes? - BYJU'S
“Enzymes can be defined as biological polymers that catalyze biochemical reactions.” The majority of enzymes are proteins with catalytic capabilities crucial to perform different processes. …
Enzymes: What They Are and How They Work - Verywell Health
Oct 22, 2024 · Enzymes are substances in the body that cause and speed up crucial chemical reactions. Enzymes’ function is to help trigger bodily processes ranging from digestion to blood …
Enzyme - Wikipedia
An enzyme (/ ˈ ɛ n z aɪ m /) is a protein that acts as a biological catalyst by accelerating chemical reactions. The molecules upon which enzymes may act are called substrates, and the enzyme …
Enzyme | Definition, Mechanisms, & Nomenclature | Britannica
May 20, 2025 · Enzyme, a catalyst that regulates the rate at which chemical reactions proceed in living organisms without itself being altered in the process. Most critically, enzymes catalyze all …
Enzymes: Function, definition, and examples - Medical News Today
Dec 8, 2023 · Enzymes provide help with facilitating chemical reactions within each cell. Since they are not destroyed during the process, a cell can reuse each enzyme repeatedly. This …
Enzymes: What Are Enzymes, Pancreas, Digestion & Liver Function
May 12, 2021 · Enzymes are proteins that help speed up chemical reactions in our bodies. Enzymes are essential for digestion, liver function and much more. Too much or too little of a …
Enzymes: principles and biotechnological applications - PMC
Enzymes are biological catalysts (also known as biocatalysts) that speed up biochemical reactions in living organisms. They can also be extracted from cells and then used to catalyse …
Enzyme: Definition, Types, Structure, Functions, & Diagram
Nov 11, 2021 · Enzymes are protein macromolecules that are necessary to initiate or speed up the rate of chemical reactions in the bodies of living organisms. The molecules on which enzymes …
Enzyme - National Human Genome Research Institute
4 days ago · An enzyme is a biological catalyst and is almost always a protein. It speeds up the rate of a specific chemical reaction in the cell. The enzyme is not destroyed during the reaction …
Enzymes - Definition, Examples, Function - Science Notes and …
Mar 25, 2025 · Enzymes are specialized proteins (and in some cases RNA molecules) that act as catalysts in living organisms. They speed up the chemical reactions required for life by …
What Are Enzymes? - BYJU'S
“Enzymes can be defined as biological polymers that catalyze biochemical reactions.” The majority of enzymes are proteins with catalytic capabilities crucial to perform different …
Enzymes: What They Are and How They Work - Verywell Health
Oct 22, 2024 · Enzymes are substances in the body that cause and speed up crucial chemical reactions. Enzymes’ function is to help trigger bodily processes ranging from digestion to blood …
