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| centrifugation in biotechnology: Centrifugal Separations in Biotechnology Wallace Woon-Fong Leung, 2020-03-13 Centrifugal Separations in Biotechnology, Second Edition, is the only book on the market devoted to centrifugal separation in biotechnology. Key topics covered include a full introduction to centrifugation, sedimentation and separation; detailed coverage of centrifuge types, including batch and semi-batch centrifuges, disk-stack and tubular decanter centrifuges; methods for increasing solids concentration; laboratory and pilot testing of centrifuges; selection and sizing centrifuges; scale-up of equipment, performance prediction and analysis of test results using numerical simulation. Centrifugal Separations in Biotechnology, Second Edition, provides guidance on troubleshooting and optimizing centrifuges, and then goes on to explore the commercial applications of centrifuges in biotechnology. It gives detailed process information and data to assist in the development of particular processes from existing systems. It is of value to professionals in the chemical, bioprocess, and biotech sectors, and all those concerned with bioseparation, bioprocessing, unit-operations and process engineering. - Provides a comprehensive guide to centrifuges, their optimal development, and their operation in the biotechnology industry - Updated throughout based on developments in industrial applications and advances in our understanding of centrifugal separations in biotechnology - Discusses applications for the separation of proteins, DNA, mitochondria, ribosomes, lysosomes and other cellular elements - Includes new sections on use of optimal polymer dosage in waste treatment, new centrifuge designs for applications in algae processing, biopharma, and more |
| centrifugation in biotechnology: Biological Centrifugation Dr John Graham, 2020-12-18 An important introduction to the use of the centrifuge in the biology laboratory, Biological Centrifugation is also useful for more experienced workers. The book describes the background and the principles behind centrifugation, including sedimentation theory. The book also considers the different types of centrifuge and other centrifuge hardware available, density gradient media and gradient technology. Although aimed primarily at the novice, this title also provides information to allow more experienced workers to modify and update existing techniques. |
| centrifugation in biotechnology: Calculations for Molecular Biology and Biotechnology Frank H. Stephenson, 2010-07-30 Calculations for Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory, Second Edition, provides an introduction to the myriad of laboratory calculations used in molecular biology and biotechnology. The book begins by discussing the use of scientific notation and metric prefixes, which require the use of exponents and an understanding of significant digits. It explains the mathematics involved in making solutions; the characteristics of cell growth; the multiplicity of infection; and the quantification of nucleic acids. It includes chapters that deal with the mathematics involved in the use of radioisotopes in nucleic acid research; the synthesis of oligonucleotides; the polymerase chain reaction (PCR) method; and the development of recombinant DNA technology. Protein quantification and the assessment of protein activity are also discussed, along with the centrifugation method and applications of PCR in forensics and paternity testing. - Topics range from basic scientific notations to complex subjects like nucleic acid chemistry and recombinant DNA technology - Each chapter includes a brief explanation of the concept and covers necessary definitions, theory and rationale for each type of calculation - Recent applications of the procedures and computations in clinical, academic, industrial and basic research laboratories are cited throughout the text New to this Edition: - Updated and increased coverage of real time PCR and the mathematics used to measure gene expression - More sample problems in every chapter for readers to practice concepts |
| centrifugation in biotechnology: Modern Analytical Ultracentrifugation Todd M. Schuster, Thomas M. Laue, 2012-12-06 There are numerous examples in the history of science when the parallel develop ments of two or more disciplines, methodologies, technologies or theoretical in sights have converged to produce significant scientific advances. The decades following the 1950s have produced several such significant advances, as a result of a convergence of developments in molecular biology and in solid state-based electronics instrumentation. Since one of these areas of significant advancement, analytical ultracentrifu gation, has been undergoing a renaissance, we thought it would be a useful activity to call upon a group of researchers who have been developing either the experi mental or theoretical aspects of the methodology and gather in one place a group of articles summarizing the current status of the field. The success of recombinant DNA methodologies at producing biologically active macromolecules of commer cial interest has evoked interests in mechanisms of function. Pursuit of the related questions has emphasized the importance of studies of macromolecular binding and interaction. Several contributions to this volume remind us that analytical ultra centrifugation is rigorously based on solid thermodynamic theory and, as such, is fully capable of providing comprehensive quantitative descriptions of molecular interactions in solution. Furthermore, a number of the chapters provide examples, along with innovative methods for carrying out these characterizations. The past decade has seen several developments that reflect the rebirth of interest in analytical ultracentrifugation. |
| centrifugation in biotechnology: Molecular Biology of the Cell , 2002 |
| centrifugation in biotechnology: Biosafety in Industrial Biotechnology P. Hambleton, T. Salusbury, 1994-09-30 As an industry, biotechnology may be likened to the Hymn Book, being both ancient and modern. Whereas activities such as baking, brewing, the fermenting of foods date from our earliest attempts to control and utilise the environment, the application of recombinant DNA technology is recognised as being at the forefront of novel industrial development. Perhaps because of its association with processing foodstuffs together with the benefits derived from applications in the early organic chemistry and pharmaceutical industries, biotechnology has been regarded as being inherently safe. Yet unlike other modern industries, such as chemical and nuclear, where regulation has followed from incidents or accidents, modern biotechnology has been subject to close scrutiny and regulation almost from its inception. The process of regulation itself is somewhat unusual in that it was initially self-imposed by the very scientists who developed the fundamental techniques of recombinant DNA technology. They recognised the signific ance of their development but were concerned of the effects on humans and the environment of uncontrolled application of the new, powerful technology. Concern about the possible consequences of genetic manipula tion has undoubtedly been the driving force behind the regulations that are now in place in many parts of the world and which are the subject of this book. Safety issues in the biotechnology industry can be categorised under three headings: worker, environmental and consumer (product) safety. |
| centrifugation in biotechnology: Centrifugal Separations in Biotechnology Wallace Woon-Fong Leung, 2007-08-16 This book is the first devoted to centrifugal separation in biotechnology. It is of value toprofessionals in the chemical, bioprocess, and biotech sectors, and all those concerned with bioseparation, bioprocessing, unit-operations and process engineering.Key topics covered include a full introduction to centrifugation, sedimentation and separation; detailed coverage of centrifuge types, including batch and semi-batch centrifuges, disk-stack and tubular decanter centrifuges; methods for increasing solids concentration; laboratory and pilot testing of centrifuges; selection and sizing centrifuges; scale-up of equipment, performance prediction and analysis of test results using numerical simulation. - A comprehensive guide to centrifuges, their optimal development and operation in the biotechnology industry - Applications for the separation of proteins, DNA, mitochondria, ribosomes, lysosomes and other cellular elements - Provides detailed process information and data to assist in the development of particular processes from existing systems - Explores the commercial applications of centrifuges in biotechnology - Guidance on troubleshooting and optimizing centrifuges |
| centrifugation in biotechnology: Biotechnology: Plant biotechnology, animal cell culture, immunobiotechnology Jack G. Chirikjian, Edward Clement Kisailus, 1995 Vol. I: The work presented in these two volumes is the collaborative effort of over twenty undergraduate science faculty, whose common goal was to develop a text of unique and flexible laboratory activities focusing on the theory and practice of biotechnology for undergraduate students. The books are designed to provide flexibility for easy integration into any course in the life sciences with an experimental emphasis. |
| centrifugation in biotechnology: Pharmaceutical Biotechnology Daan J. A. Crommelin, Robert D. Sindelar, Bernd Meibohm, 2013-10-22 This introductory text explains both the basic science and the applications of biotechnology-derived pharmaceuticals, with special emphasis on their clinical use. It serves as a complete one-stop source for undergraduate/graduate pharmacists, pharmaceutical science students, and for those in the pharmaceutical industry. The Fourth Edition will completely update the previous edition, and will also include additional coverage on the newer approaches such as oligonucleotides, siRNA, gene therapy and nanotech. |
| centrifugation in biotechnology: Basic Separation Techniques in Biochemistry R O Okotore, 1998 Basic Separation Techniques In Biochemistry Provides Information On The Basic Separation Techniques Most Commonly Employed In Biochemical Research.The Basic Principles And Applications Of The Routine Methods For The Fractionation Of Subcellular Macromolecules Have Been Discussed In Simple And Comprehensive Manner.The Methodology Of Each Technique Is Presented In A Precise And Concise Way For Meaningful Understanding To A Beginner Student. The Book Is In Eight Chapters, Each With Statement Of Objectives. The Book Will Prove Of Value To Undergraduate Students Of Biochemistry, Chemistry And Biology As Supplementary Reading Text To More Advanced Texts In Laboratory Techniques. |
| centrifugation in biotechnology: Handbook of Pharmaceutical Biotechnology Shayne Cox Gad, 2007-05-23 A practical overview of a full rangeof approaches to discovering, selecting, and producing biotechnology-derived drugs The Handbook of Pharmaceutical Biotechnology helps pharmaceutical scientists develop biotech drugs through a comprehensive framework that spans the process from discovery, development, and manufacturing through validation and registration. With chapters written by leading practitioners in their specialty areas, this reference: Provides an overview of biotechnology used in the drug development process Covers extensive applications, plus regulations and validation methods Features fifty chapters covering all the major approaches to the challenge of identifying, producing, and formulating new biologically derived therapeutics With its unparalleled breadth of topics and approaches, this handbook is a core reference for pharmaceutical scientists, including development researchers, toxicologists, biochemists, molecular biologists, cell biologists, immunologists, and formulation chemists. It is also a great resource for quality assurance/assessment/control managers, biotechnology technicians, and others in the biotech industry. |
| centrifugation in biotechnology: Introduction to Instrumentation in Life Sciences Prakash Singh Bisen, Anjana Sharma, 2012-09-26 Instrumentation is central to the study of physiology and genetics in living organisms, especially at the molecular level. Numerous techniques have been developed to address this in various biological disciplines, creating a need to understand the physical principles involved in the operation of research instruments and the parameters required in u |
| centrifugation in biotechnology: The Impact of Food Bioactives on Health Kitty Verhoeckx, Paul Cotter, Iván López-Expósito, Charlotte Kleiveland, Tor Lea, Alan Mackie, Teresa Requena, Dominika Swiatecka, Harry Wichers, 2015-04-29 “Infogest” (Improving Health Properties of Food by Sharing our Knowledge on the Digestive Process) is an EU COST action/network in the domain of Food and Agriculture that will last for 4 years from April 4, 2011. Infogest aims at building an open international network of institutes undertaking multidisciplinary basic research on food digestion gathering scientists from different origins (food scientists, gut physiologists, nutritionists...). The network gathers 70 partners from academia, corresponding to a total of 29 countries. The three main scientific goals are: Identify the beneficial food components released in the gut during digestion; Support the effect of beneficial food components on human health; Promote harmonization of currently used digestion models Infogest meetings highlighted the need for a publication that would provide researchers with an insight into the advantages and disadvantages associated with the use of respective in vitro and ex vivo assays to evaluate the effects of foods and food bioactives on health. Such assays are particularly important in situations where a large number of foods/bioactives need to be screened rapidly and in a cost effective manner in order to ultimately identify lead foods/bioactives that can be the subject of in vivo assays. The book is an asset to researchers wishing to study the health benefits of their foods and food bioactives of interest and highlights which in vitro/ex vivo assays are of greatest relevance to their goals, what sort of outputs/data can be generated and, as noted above, highlight the strengths and weaknesses of the various assays. It is also an important resource for undergraduate students in the ‘food and health’ arena. |
| centrifugation in biotechnology: Subcellular Fractionation Paul R. Pryor, 2015 Eukaryotic cells are remarkably complex structures, containing a vast repertoire of macromolecules, organelles, and other compartments that orchestrate the tasks required for life. For in-depth studies of their function and composition, reliable methods for the isolation of specific subcellular structures are often required. This laboratory manual provides step-by-step protocols for the extraction of subcellular components from animal tissues, yeasts, plants, and cultured cells. Each chapter focuses on a particular eukaryotic organelle, vesicle, membrane, or macromolecular complex. Strategies for breaking cells while maintaining the structural and functional integrity of the component of interest, enriching for that component based on its physical and biochemical characteristics, and monitoring and ensuring the success of the purification procedure are provided. The contributors describe both traditional approaches (e.g., density gradient centrifugation) and innovative techniques (e.g., the use of SPIONs) for isolating subcellular constituents. This manual is therefore an essential laboratory resource for all cell biologists seeking a comprehensive collection of dependable subcellular fractionation methods. |
| centrifugation in biotechnology: Compendium of Biomedical Instrumentation Raghbir Singh Khandpur, 2019-11-18 An essential reference filled with 400 of today's current biomedical instruments and devices Designed mainly for the active bio-medical equipment technologists involved in hands-on functions like managing these technologies by way of their usage, operation & maintenance and those engaged in advancing measurement techniques through research and development, this book covers almost the entire range of instruments and devices used for diagnosis, imaging, analysis, and therapy in the medical field. Compiling 400 instruments in alphabetical order, it provides comprehensive information on each instrument in a lucid style. Each description in Compendium of Biomedical Instrumentation covers four aspects: purpose of the instrument; principle of operation, which covers physics, engineering, electronics, and data processing; brief specifications; and major applications. Devices listed range from the accelerometer, ballistocardiograph, microscopes, lasers, and electrocardiograph to gamma counter, hyperthermia system, microtome, positron emission tomography, uroflowmeter, and many more. Covers almost the entire range of medical instruments and devices which are generally available in hospitals, medical institutes at tertiary, secondary, and peripheral level facilities Presents broad areas of applications of medical instruments/technology, including specialized equipment for various medical specialties, fully illustrated with figures & photographs Contains exhaustive description on state of the art instruments and also includes some generation old legacy instruments which are still in use in some medical facilities. Compendium of Biomedical Instrumentation is a must-have resource for professionals and undergraduate and graduate students in biomedical engineering, as well as for clinical engineers and bio-medical equipment technicians. |
| centrifugation in biotechnology: Downstream Processing of Proteins Mohamed A. Desai, 2008-02-05 Considerable effort and time is allocated to introducing cell culture and fermentation technology to undergraduate students in academia, generally through a range of courses in industrial biotechnology and related disciplines. Similarly, a large number of textbooks are available to describe the appli- tions of these technologies in industry. However, there has been a general lack of appreciation of the significant developments in downstream processing and isolation technology, the need for which is largely driven by the stringent re- latory requirements for purity and quality of injectable biopharmaceuticals. This is particularly reflected by the general absence of coverage of this s- ject in many biotechnology and related courses in educational institutions. For a considerable while I have felt that there is increasing need for an introductory text to various aspects of downstream processing, particularly with respect to the needs of the biopharmaceutical and biotechnology ind- try. Although there are numerous texts that cover various aspects of protein purification techniques in isolation, there is a need for a work that covers the broad range of isolation technology in an industrial setting. It is anticipated that Downstream Processing of Proteins: Methods and Protocols will play a small part in filling this gap and thus prove a useful contribution to the field. It is also designed to encourage educational strategists to broaden the coverage of these topics in industrial biotechnology courses by including accounts of this important and rapidly developing element of the industrial process. |
| centrifugation in biotechnology: Isolation and Purification of Proteins Rajni Hatti-Kaul, Bo Mattiasson, 2003-02-05 This publication details the isolation of proteins from biological materials, techniques for solid-liquid separation, concentration, crystallization, chromatography, scale-up, process monitoring, product formulation, and regulatory and commercial considerations in protein production. The authors discuss the release of protein from a biological host, selectivity in affinity chromatography, precipitation of proteins (both non-specific and specific), extraction for rapid protein isolation, adsorption as an initial step for the capture of proteins, scale-up and commercial production of recombinant proteins, and process monitoring in downstream processing. |
| centrifugation in biotechnology: Comprehensive Sampling and Sample Preparation Josep M. Bayona, Paola Dugo, X. Chris Le, Hian Kee Lee, Xing-Fang Li, Heather Lord, 2012-12-31 Comprehensive Sampling and Sample Preparation is a complete treatment of the theory and methodology of sampling in all physical phases and the theory of sample preparation for all major extraction techniques. It is the perfect starting point for researchers and students to design and implement their experiments and support those experiments with quality-reviewed background information. In its four volumes, fundamentals of sampling and sample preparation are reinforced through broad and detailed sections dealing with Biological and Medical, Environmental and Forensic, and Food and Beverage applications. The contributions are organized to reflect the way in which analytical chemists approach a problem. It is intended for a broad audience of analytical chemists, both educators and practitioners of the art and can assist in the preparation of courses as well in the selection of sampling and sample preparation techniques to address the challenges at hand. Above all, it is designed to be helpful in learning more about these topics, as well as to encourage an interest in sampling and sample preparation by outlining the present practice of the technology and by indicating research opportunities. Sampling and Sample preparation is a large and well-defined field in Analytical Chemistry, relevant for many application areas such as medicine, environmental science, biochemistry, pharmacology, geology, and food science. This work covers all these aspects and will be extremely useful to researchers and students, who can use it as a starting point to design and implement their experiments and for quality-reviewed background information There are limited resources that Educators can use to effectively teach the fundamental aspects of modern sample preparation technology. Comprehensive Sampling and Sample Preparation addresses this need, but focuses on the common principles of new developments in extraction technologies rather than the differences between techniques thus facilitating a more thorough understanding Provides a complete overview of the field. Not only will help to save time, it will also help to make correct assessments and avoid costly mistakes in sampling in the process Sample and sample preparation are integral parts of the analytical process but are often less considered and sometimes even completely disregarded in the available literature. To fill this gap, leading scientists have contributed 130 chapters, organized in 4 volumes, covering all modern aspects of sampling and liquid, solid phase and membrane extractions, as well as the challenges associated with different types of matrices in relevant application areas |
| centrifugation in biotechnology: Methods of Molecular Analysis in the Life Sciences Andreas Hofmann, Anne Simon, Tanja Grkovic, Malcolm Jones, 2014-06-19 An accessible overview of the most popular and cutting-edge methods for studying the properties of molecules and their interactions. |
| centrifugation in biotechnology: Analytical Techniques in Biochemistry Mahin Basha, 2020 This book provides a comprehensive overview of the major biochemical analytical techniques, with detailed descriptions of the instrumentation and applications. The contributions, which each focus on a specific technique, are based on a thorough review and analysis of the current literature as well as the authors' experiences in the lab. Divided into nine parts, the book provides insights into basic separation techniques like sedimentation, filtration and centrifugation, as well as analytical techniques such as spectrophotometry, chromatography, electrophoresis, immuno-techniques, radioactivity and microscopy. . |
| centrifugation in biotechnology: Current Developments in Biotechnology and Bioengineering Ashok Pandey, Jose Antonio Couto Teixeira, 2016-09-19 Current Developments in Biotechnology and Bioengineering: Foundations of Biotechnology and Bioengineering is a package of nine books that compile the latest ideas from across the entire arena of biotechnology and bioengineering. This volume focuses on the underlying principles of biochemistry, microbiology, fermentation technology, and chemical engineering as interdisciplinary themes, constructing the foundation of biotechnology and bioengineering. - Provides state-of-art information on basics and fundamental principles of biotechnology and bioengineering - Supports the education and understanding of biotechnology education and R&D - Contains advanced content for researchers engaged in bioengineering research |
| centrifugation in biotechnology: Purification of Biotechnological Products Adalberto Pessoa Jr, Beatriz Vahan Kilikian, Paul Long, 2024-05-23 This outstanding text focuses on providing professionals and students working in the pharmaceutical and biotechnology field with the background necessary for developing of a product or process and with the necessary rigor required by federal regulatory agencies in the pharmaceutical industry. The material will enable teachers, lecturers and professors in biotechnology to prepare courses on basic concepts and applications for the purification of biotechnological products of industrial interest. These can be applied in practice, for example, with projects on purification development on an industrial scale or useful unit operations for the development of bioproducts of commercial interest. Features: Purification and development of new bioproducts and improvement of those being produced Provides a background and concepts on the purification of biomolecules and with an industrial perspective It allows professionals to understand the entire process of developing a biopharmaceutical or bio-food, from bench to industry in biotechnology; one of the fastest-growing sectors of the economy It promotes the dissemination of information in a didactic way which is of paramount importance for interdisciplinary fields It enables the reader to follow step-by-step stages of the development of a new biopharmaceutical, and allows the optimization of existing processes |
| centrifugation in biotechnology: Bioseparations Paul A. Belter, E. L. Cussler, Wei-Shou Hu, 1988-02-22 Offers a concise introduction to the separation and purification of biochemicals. Bridges two scientific cultures, providing an introduction to bioseparations for scientists with no background in engineering and for engineers with little grounding in biology. The authors supplement the ideas by simple worked examples, making the techniques of bioseparations easy to learn. Discusses removal of insolubles, product isolation, purification and polishing. |
| centrifugation in biotechnology: Drug and Pharmaceutical Biotechnology Mr. Rohit Manglik, 2024-07-04 EduGorilla Publication is a trusted name in the education sector, committed to empowering learners with high-quality study materials and resources. Specializing in competitive exams and academic support, EduGorilla provides comprehensive and well-structured content tailored to meet the needs of students across various streams and levels. |
| centrifugation in biotechnology: Biotechnology David P. Clark, Nanette J. Pazdernik, 2009 Unlike most textbooks on this subject, Biotechnology approaches modern biotechnology from a molecular basis. Using straightforward, less technical jargon, Clark and Pazdernik introduce students to the topics and walk them through the process as it evolves into a more specific detailed principle. This up-to-date text covers a wide realm of topics that are encountered in current media and movies. One of the chapters covers the burgeoning field of nanobiotechnology, stimulating the student to think about biotechnology from a new and much smaller point of view. Another chapter explains the real biotechnology behind crime scene investigations portrayed so dramatically on the hit show CSI. In addition, students will learn about the biotechnology behind making vaccines, genetically-modified plants, stem-cell research, gene therapy, and aging, among many other topics that are part of mainstream media coverage. Students will also learn the molecular basis for many viral diseases, cancer, and bacterial diseases that are bound to affect them or other family members. Finally, the text includes a very thought-provoking chapter on the bioethics of these new advances and applications of today's world of biotechnology, which stimulates the student to think rather than memorize.--BOOK JACKET. |
| centrifugation in biotechnology: Laboratory Manual for Biotechnology and Laboratory Science Lisa A. Seidman, Mary Ellen Kraus, Diana Lietzke Brandner, Jeanette Mowery, 2022-12-23 Provides the basic laboratory skills and knowledge to pursue a career in biotechnology. Written by four biotechnology instructors with over 20 years of teaching experience, it incorporates instruction, exercises, and laboratory activities that the authors have been using and perfecting for years. These exercises and activities help students understand the fundamentals of working in a biotechnology laboratory. Building skills through an organized and systematic presentation of materials, procedures, and tasks, the manual explores overarching themes that relate to all biotechnology workplaces including forensic, clinical, quality control, environmental, and other testing laboratories. Features: Provides clear instructions and step-by-step exercises to make learning the material easier for students (There are Lab Notes for Instructors in the Support Material (see tab below) Emphasizes fundamental laboratory skills that prepare students for the industry Builds students’ skills through an organized and systematic presentation of materials, procedures, and tasks Updates reflect recent innovations and regulatory requirements to ensure students stay up to date Supplies skills suitable for careers in forensic, clinical, quality control, environmental, and other testing laboratories |
| centrifugation in biotechnology: Centrifugal Separations in Molecular and Cell Biology G.D. Birnie, D. Rickwood, 2014-06-28 Centrifugal Separations in Molecular and Cell Biology focuses on the application of modern centrifugation technology in molecular and cell biology, including the separation and fractionation of biological particles by centrifugation on the preparative and analytical scales. The selection first covers the principles and practices of centrifugation and the bases of centrifugal separations. Discussions focus on the basic concepts of sedimentation theory, centrifugation methods, designing centrifugation experiments, care of centrifuges and rotors, and statistical estimation of molecular parameters. The book also ponders on the practical aspects of rate-zonal centrifugation, including gradient materials, density and viscosity of glycerol solutions, and resolution and gradient shape. The publication examines fractionations in zonal rotors and the quantitative aspects of rate-zonal centrifugation. The text then reviews isopycnic centrifugation in ionic media and analytical centrifugation. Topics include separation by isopycnic banding, large-scale preparative procedures, and density-gradient solutes. The selection is a valuable reference for readers interested in centrifugation technology. |
| centrifugation in biotechnology: Process Scale Purification of Antibodies Uwe Gottschalk, 2011-09-20 Traditional column chromatography dominates current purification technology, and many of the productivity gains that have been achieved have relied on upscaling such devices. However, this comes with a cost penalty and the pharmaceutical industry has reached the point at which further upscaling becomes economically unsupportable. This book offers a broad-based reassessment of old and new purification methods, incorporating an analysis of innovative new trends in purification. The book has wide coverage of different antibody purification strategies and brings together top-tier experts to address problems in process-scale antibody purification. |
| centrifugation in biotechnology: Preparative Centrifugation David Rickwood, 1992 While the theoretical basis of centrifugal separations remains the same, recent years have seen dramatic changes in both the design of centrifuges and the range of people that use them. This book reflects these changing uses of preparative centrifuges by providing detailed protocols covering all of the different types of separation from DNA to cells. Guidelines are also given to help the reader devise new types of separation protocols. The book includes program source code for calculation and simulation programs that are invaluable for quantitative centrifugation methods. In addition, this volume contains extensive appendices of valuable data that are required by everyone using centrifuges as part of their research work. Preparative Centrifugation: A Practical Approach contains a wealth of guidelines, protocols, and practical advice that will be of direct use to experienced researchers and novices alike in virtually every area of biological research. |
| centrifugation in biotechnology: Biotechnology Handbook NIIR Board, 2003-08-09 Biotechnology is a field of applied biology that involves the use of living organisms and bioprocesses in engineering, technology, medicine and other fields requiring bio products. Biotechnology also utilizes these products for manufacturing purpose. Modern use of similar terms includes genetic engineering as well as cell and tissue culture technologies. Biotechnology draws on the pure biological sciences and in many instances is also dependent on knowledge and methods from outside the sphere of biology. Conversely, modern biological sciences are intimately entwined and dependent on the methods developed through biotechnology and what is commonly thought of as the life sciences industry. It has a major application in modern brewing technology which includes the production of whisky, traditional fermented soybean foods bacterial biomass, cheese starters, cheese technology, L glutamic acid fermentation etc. Biotechnology and cell molecular biology have developed and emerged in to a major discipline during last two decades. Biotechnology is also used to recycle, treat waste, microbial treatment and utilization a waste. The growing global demand for biotechnology products, India has rich biodiversity that drives its clinical trials industry and forms a strong base for pharmaceutical research. In recent years, the worldwide biotechnology based products market has grown at an annual average rate of 15%. This book majorly deals with introduction to basic biotechnology, downstream processing in biotechnology, modern brewing technology, industrial chemicals, biochemical and fuels, microbial flavours and fragrances, biodegradation of non cellulosic wastes for environmental conservation and fuel production, landfills for treatment of solid wastes etc. This book also consists of addresses of machinery suppliers, addresses of chemical suppliers, list of universities, conducting Biotechnology courses in the directory section. This is a unique book, concise, up to date resource offering an innovative, adoptive and valuable presentation of the subject. It covers all important biotechnological topics of industrial and academic interests. This book will be very use full for industry people, students, and libraries and for those who want to venture in to manufacturing of biotechnological products. 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| centrifugation in biotechnology: Downstream Process Technology: A New Horizon In Biotechnology Krishna Kant Prasad, Nooralabettu Krishna Prasad, 2010-01-30 Today, biochemical process industry demands fast and economic processes for the partitioning and purification of biomolecules that give high yield and high purity of the product. An integral and cost intensive part of these processes is associated with downstream processing for product isolation and purification. The aim of this comprehensive text is to provide an insightful overview of the whole aspects of downstream processing for biochemical product recovery. Intended for undergraduate and postgraduate students of biotechnology and chemical engineering, this self-contained text includes the chapters based on the recent developments in the industry and academics. It covers the importance of the downstream processing in terms of its relevancy to modern days ever-changing consumer needs, process design criteria relevance to set objectives, and physicochemical factors that help to formulate the strategy to develop a configuration among the raw material, methodology and instruments. This overview is followed by different downstream processing steps. The text concludes with the discussion on stabilization of the product to improve the shelf life of the product. Key Features Includes detailed biological, mathematical, chemical and physical aspects of downstream processing. Distinguishes downstream processing from analytical bioseparation. Contains numerous illustrations and solved problems. |
| centrifugation in biotechnology: Analytical Ultracentrifugation in Biochemistry and Polymer Science Stephen E. Harding, Arthur J. Rowe, John C. Horton, 1992 This is the first book of its kind to appear for nearly two decades and gives as comprehensive a coverage as is possible of the present state-of-the-art. |
| centrifugation in biotechnology: Bioluminescence: Fundamentals and Applications in Biotechnology - Volume 3 Gérald Thouand, Robert Marks, 2016-01-12 This book review series presents current trends in modern biotechnology. The aim is to cover all aspects of this interdisciplinary technology where knowledge, methods and expertise are required from chemistry, biochemistry, microbiology, genetics, chemical engineering and computer science. Volumes are organized topically and provide a comprehensive discussion of developments in the respective field over the past 3-5 years. The series also discusses new discoveries and applications. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification. In general, special volumes are edited by well-known guest editors. The series editor and publisher will however always be pleased to receive suggestions and supplementary information. Manuscripts are accepted in English. /div |
| centrifugation in biotechnology: Process Scale Purification of Antibodies Uwe Gottschalk, 2017-03-02 Promoting a continued and much-needed renaissance in biopharmaceutical manufacturing, this book covers the different strategies and assembles top-tier technology experts to address the challenges of antibody purification. • Updates existing topics and adds new ones that include purification of antibodies produced in novel production systems, novel separation technologies, novel antibody formats and alternative scaffolds, and strategies for ton-scale manufacturing • Presents new and updated discussions of different purification technologies, focusing on how they can address the capacity crunch in antibody purification • Emphasizes antibodies and innovative chromatography methods for processing |
| centrifugation in biotechnology: Centrifugation David Rickwood, T. Ford, J. Steensgaard, 1994-06-14 Centrifugation is at the center of most fractionation methods in a wide range of disciplines. This volume provides the core data required by researchers carrying out any centrifugal separation, whether working with cells, organelles or macromolecules. |
| centrifugation in biotechnology: Risk Management Applications in Pharmaceutical and Biopharmaceutical Manufacturing Hamid Mollah, Harold Baseman, Mike Long, 2013-03-18 Sets forth tested and proven risk management practices in drug manufacturing Risk management is essential for safe and efficient pharmaceutical and biopharmaceutical manufacturing, control, and distribution. With this book as their guide, readers involved in all facets of drug manufacturing have a single, expertly written, and organized resource to guide them through all facets of risk management and analysis. It sets forth a solid foundation in risk management concepts and then explains how these concepts are applied to drug manufacturing. Risk Management Applications in Pharmaceutical and Biopharmaceutical Manufacturing features contributions from leading international experts in risk management and drug manufacturing. These contributions reflect the latest research, practices, and industry standards as well as the authors' firsthand experience. Readers can turn to the book for: Basic foundation of risk management principles, practices, and applications Tested and proven tools and methods for managing risk in pharmaceutical and biopharmaceutical product manufacturing processes Recent FDA guidelines, EU regulations, and international standards governing the application of risk management to drug manufacturing Case studies and detailed examples demonstrating the use and results of applying risk management principles to drug product manufacturing Bibliography and extensive references leading to the literature and helpful resources in the field With its unique focus on the application of risk management to biopharmaceutical and pharmaceutical manufacturing, this book is an essential resource for pharmaceutical and process engineers as well as safety and compliance professionals involved in drug manufacturing. |
| centrifugation in biotechnology: Comprehensive Biotechnology , 2019-07-17 Comprehensive Biotechnology, Third Edition, Six Volume Set unifies, in a single source, a huge amount of information in this growing field. The book covers scientific fundamentals, along with engineering considerations and applications in industry, agriculture, medicine, the environment and socio-economics, including the related government regulatory overviews. This new edition builds on the solid basis provided by previous editions, incorporating all recent advances in the field since the second edition was published in 2011. Offers researchers a one-stop shop for information on the subject of biotechnology Provides in-depth treatment of relevant topics from recognized authorities, including the contributions of a Nobel laureate Presents the perspective of researchers in different fields, such as biochemistry, agriculture, engineering, biomedicine and environmental science |
| centrifugation in biotechnology: Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology Keith Wilson, Andreas Hofmann, John M. Walker, Samuel Clokie, 2018-04-19 A major update of a best-selling textbook that introduces students to the key experimental and analytical techniques underpinning life science research. |
| centrifugation in biotechnology: Biotechnology for Pharmaceutical Sciences Ana Catarina Silva, 2024-06-03 This book provides a compact and straightforward overview of the main concepts and applications of Pharmaceutical Biotechnology. The author collates lecture notes on Pharmaceutical Biotechnology to introduce the topic to graduate students in the fields of Pharmaceutical Sciences, Biochemistry, Biotechnology and Industrial Biotechnology, Microbiology, and Medicinal Chemistry. The book starts with an overview of the biotechnological processes needed to develop biological and biosimilar medicines. Next, the author addresses the development and use of advanced therapy medicinal products (ATMPs), including topics such as cell and gene therapies, regenerative medicine and the regulatory issues of biological medicines and ATMPs. Finally, the author explores the limitations of administering biopharmaceuticals, discussing protein and nucleic acid stability issues, potential routes of administration and strategies for improving the bioavailability of biologics and ATMPs. This book captures the latest developments in the field and offers a practical perspective on the topic, serving as a valuable introduction resource not only for graduate students but also for researchers. The basis of the English translation of this book, originally in Portuguese, was facilitated by artificial intelligence. The content was later revised by the author for accuracy. |
| centrifugation in biotechnology: Phycobiotechnology Jeyabalan Sangeetha, Devarajan Thangadurai, Saniyasi Elumalai, Shivasharana Chandrabanda Thimmappa, 2021-03-01 Named #1 of 15 Best New Biotechnology Books to Read in 2021 by BookAuthority. This volume explores and explains the vast uses and benefits of algae as food, feed, and fuel. It covers the most advanced applications of algae in the food and feed industries and for environmental sustainability. With chapters written by experts and which were extensively reviewed by many well-known subject experts and professionals, Phycobiotechnology: Biodiversity and Biotechnology of Algae and Algal Products for Food, Feed, and Fuel provides an abundance of valuable information. Algae are a genetically diverse group of organisms with a wide range of physiological and biochemical characteristics that have unique capabilities in the fields of agriculture, pharmaceuticals, industry, and environment. Algae hold the potential to become the planet’s next major source of energy and a vital part of the solution for climate change and dependence on fossil fuels. Many varieties of algae are also known to be an abundant source of vitamins, minerals, and other nutrients that can boost the human immune system. |
Centrifugation - Wikipedia
Centrifugation is a mechanical process which involves the use of the centrifugal force to separate particles from a solution according to their size, shape, density, medium viscosity and rotor …
Centrifuge: Principle, Parts, Types, and Applications - Microbe Notes
Nov 5, 2024 · Centrifugation is a term used to describe a method of separating mixtures using spinning and centrifugal force. Several characteristics can separate particles during …
Centrifugation - Lab Tests Guide
Sep 1, 2023 · Centrifugation is a laboratory technique that involves the separation of particles from a liquid or suspension based on their density and size differences using the application of …
Centrifugation: Lab Techniques, Examples and Types | Corning
Centrifugation is a cornerstone lab technique in various scientific fields which revolutionizes the separation and examination of diverse biological samples. This technique employs high-speed …
Centrifugation: Definition, Principle, Types, and 3 Reliable ...
Apr 26, 2023 · By rapidly spinning molecules in solution around an axis (in a centrifuge rotor), centrifugation is a technique for separating molecules with various densities. The mechanical …
Basics: Centrifugal Force & Centrifuge Type - Eppendorf US
Jul 22, 2019 · What is a centrifuge, and how does a centrifuge work? Centrifugation is a technique that helps to separate mixtures by applying centrifugal force. A centrifuge is a device, …
Centrifugation - an overview | ScienceDirect Topics
Centrifugation is a mechanical process that utilizes an applied centrifugal force field to separate the components of a mixture according to density and/or particle size. The principles that …
Centrifuge: Principle, Types, Application, Limitation - Science Info
Feb 8, 2024 · Centrifugation relies on the behavior of particles in a solution, where those with higher density settle and those with lower density rise. The process utilizes a powerful …
What Centrifugation Is and Why It's Used - ThoughtCo
Centrifugation uses spinning to separate substances based on their density. Centrifuges have many uses, from medical labs to amusement park rides. Different types of centrifuges work …
Principle, Parts, Types, Applications - Biology Notes Online
Feb 13, 2025 · Centrifugation is a process that uses centrifugal force to separate substances of different densities or to separate particles of different sizes in a mixture. It is commonly used in …
Centrifugation - Wikipedia
Centrifugation is a mechanical process which involves the use of the centrifugal force to separate particles from a solution according to their size, shape, density, medium viscosity and rotor …
Centrifuge: Principle, Parts, Types, and Applications - Microbe Notes
Nov 5, 2024 · Centrifugation is a term used to describe a method of separating mixtures using spinning and centrifugal force. Several characteristics can separate particles during …
Centrifugation - Lab Tests Guide
Sep 1, 2023 · Centrifugation is a laboratory technique that involves the separation of particles from a liquid or suspension based on their density and size differences using the application of …
Centrifugation: Lab Techniques, Examples and Types | Corning
Centrifugation is a cornerstone lab technique in various scientific fields which revolutionizes the separation and examination of diverse biological samples. This technique employs high-speed …
Centrifugation: Definition, Principle, Types, and 3 Reliable ...
Apr 26, 2023 · By rapidly spinning molecules in solution around an axis (in a centrifuge rotor), centrifugation is a technique for separating molecules with various densities. The mechanical …
Basics: Centrifugal Force & Centrifuge Type - Eppendorf US
Jul 22, 2019 · What is a centrifuge, and how does a centrifuge work? Centrifugation is a technique that helps to separate mixtures by applying centrifugal force. A centrifuge is a device, generally …
Centrifugation - an overview | ScienceDirect Topics
Centrifugation is a mechanical process that utilizes an applied centrifugal force field to separate the components of a mixture according to density and/or particle size. The principles that govern …
Centrifuge: Principle, Types, Application, Limitation - Science Info
Feb 8, 2024 · Centrifugation relies on the behavior of particles in a solution, where those with higher density settle and those with lower density rise. The process utilizes a powerful …
What Centrifugation Is and Why It's Used - ThoughtCo
Centrifugation uses spinning to separate substances based on their density. Centrifuges have many uses, from medical labs to amusement park rides. Different types of centrifuges work based on …
Principle, Parts, Types, Applications - Biology Notes Online
Feb 13, 2025 · Centrifugation is a process that uses centrifugal force to separate substances of different densities or to separate particles of different sizes in a mixture. It is commonly used in …
